YKL185W / ASH1

Zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL185W / ASH1

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.15|P-value:8.23E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:2.68|P-value:6.56E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-2.79|P-value:4.11E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.40|P-value:2.28E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:2.65|P-value:7.54E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:2.59|P-value:9.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:4.41|P-value:6.31E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:2.71|P-value:5.76E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.45|P-value:1.75E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.51|P-value:1.31E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.00|P-value:1.62E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.10|P-value:1.05E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.41|P-value:5.97E-8 ID:SGTC_518|Compound:1021-0119|FD-Score:-2.79|P-value:4.08E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:2.60|P-value:9.27E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-2.59|P-value:9.56E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:2.77|P-value:4.53E-4 ID:SGTC_917|Compound:harmaline|FD-Score:2.69|P-value:6.38E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.25|P-value:5.02E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.20|P-value:6.38E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.39|P-value:4.86E-11 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.36|P-value:6.39E-11 ID:SGTC_1718|Compound:roemerine|FD-Score:4.59|P-value:1.93E-8 ID:SGTC_1754|Compound:st042065|FD-Score:4.09|P-value:4.91E-7 ID:SGTC_1818|Compound:st052136|FD-Score:2.84|P-value:3.31E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-2.66|P-value:6.99E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-2.61|P-value:8.74E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.49|P-value:1.44E-5 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:2.61|P-value:8.86E-4 ID:SGTC_2141|Compound:5621638|FD-Score:2.70|P-value:6.03E-4 ID:SGTC_2164|Compound:5685496|FD-Score:2.77|P-value:4.51E-4 ID:SGTC_2308|Compound:7584454|FD-Score:-3.01|P-value:1.55E-4 ID:SGTC_2440|Compound:5628896|FD-Score:5.01|P-value:9.64E-10 ID:SGTC_2442|Compound:5158906|FD-Score:3.53|P-value:1.20E-5 ID:SGTC_2443|Compound:5493264|FD-Score:-3.57|P-value:9.21E-6 ID:SGTC_2445|Compound:5742597|FD-Score:-2.88|P-value:2.73E-4 ID:SGTC_2469|Compound:5655019|FD-Score:-2.91|P-value:2.45E-4 ID:SGTC_2474|Compound:5321570|FD-Score:-2.68|P-value:6.49E-4 ID:SGTC_2477|Compound:5967686|FD-Score:-2.82|P-value:3.52E-4 ID:SGTC_2484|Compound:5809818|FD-Score:2.89|P-value:2.62E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.52|P-value:1.23E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.98|P-value:1.75E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:3.51|P-value:1.28E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-3.03|P-value:1.37E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.49|P-value:1.41E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.11|P-value:9.32E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:3.24|P-value:5.12E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:-3.18|P-value:6.73E-5 ID:SGTC_2808|Compound:7946468|FD-Score:2.93|P-value:2.24E-4 ID:SGTC_2930|Compound:9013230|FD-Score:2.65|P-value:7.60E-4 ID:SGTC_2937|Compound:9044753|FD-Score:-3.47|P-value:1.60E-5 ID:SGTC_2987|Compound:9018338|FD-Score:4.25|P-value:1.72E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.24|P-value:4.97E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-2.76|P-value:4.60E-4 ID:SGTC_3231|Compound:9132313|FD-Score:-2.62|P-value:8.23E-4 ID:SGTC_3304|Compound:9125678|FD-Score:2.74|P-value:5.07E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.15|P-value:8.23E-5 ID:SGTC_101|Compound:0490-5701|FD-Score:2.68|P-value:6.56E-4 ID:SGTC_140|Compound:0062-0056|FD-Score:-2.79|P-value:4.11E-4 ID:SGTC_264|Compound:promethazine|FD-Score:-3.40|P-value:2.28E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:2.65|P-value:7.54E-4 ID:SGTC_369|Compound:0957-0393|FD-Score:2.59|P-value:9.76E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:4.41|P-value:6.31E-8 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:2.71|P-value:5.76E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.45|P-value:1.75E-5 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:3.51|P-value:1.31E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.00|P-value:1.62E-4 ID:SGTC_480|Compound:am 92016|FD-Score:3.10|P-value:1.05E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:-4.41|P-value:5.97E-8 ID:SGTC_518|Compound:1021-0119|FD-Score:-2.79|P-value:4.08E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:2.60|P-value:9.27E-4 ID:SGTC_804|Compound:1595-0054|FD-Score:-2.59|P-value:9.56E-4 ID:SGTC_853|Compound:0419-0306|FD-Score:2.77|P-value:4.53E-4 ID:SGTC_917|Compound:harmaline|FD-Score:2.69|P-value:6.38E-4 ID:SGTC_1132|Compound:3456-3614|FD-Score:3.25|P-value:5.02E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.20|P-value:6.38E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.39|P-value:4.86E-11 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.36|P-value:6.39E-11 ID:SGTC_1718|Compound:roemerine|FD-Score:4.59|P-value:1.93E-8 ID:SGTC_1754|Compound:st042065|FD-Score:4.09|P-value:4.91E-7 ID:SGTC_1818|Compound:st052136|FD-Score:2.84|P-value:3.31E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-2.66|P-value:6.99E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-2.61|P-value:8.74E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.49|P-value:1.44E-5 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:2.61|P-value:8.86E-4 ID:SGTC_2141|Compound:5621638|FD-Score:2.70|P-value:6.03E-4 ID:SGTC_2164|Compound:5685496|FD-Score:2.77|P-value:4.51E-4 ID:SGTC_2308|Compound:7584454|FD-Score:-3.01|P-value:1.55E-4 ID:SGTC_2440|Compound:5628896|FD-Score:5.01|P-value:9.64E-10 ID:SGTC_2442|Compound:5158906|FD-Score:3.53|P-value:1.20E-5 ID:SGTC_2443|Compound:5493264|FD-Score:-3.57|P-value:9.21E-6 ID:SGTC_2445|Compound:5742597|FD-Score:-2.88|P-value:2.73E-4 ID:SGTC_2469|Compound:5655019|FD-Score:-2.91|P-value:2.45E-4 ID:SGTC_2474|Compound:5321570|FD-Score:-2.68|P-value:6.49E-4 ID:SGTC_2477|Compound:5967686|FD-Score:-2.82|P-value:3.52E-4 ID:SGTC_2484|Compound:5809818|FD-Score:2.89|P-value:2.62E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:3.52|P-value:1.23E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:-2.98|P-value:1.75E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:3.51|P-value:1.28E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-3.03|P-value:1.37E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.49|P-value:1.41E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:-3.11|P-value:9.32E-5 ID:SGTC_2761|Compound:levamisole|FD-Score:3.24|P-value:5.12E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:-3.18|P-value:6.73E-5 ID:SGTC_2808|Compound:7946468|FD-Score:2.93|P-value:2.24E-4 ID:SGTC_2930|Compound:9013230|FD-Score:2.65|P-value:7.60E-4 ID:SGTC_2937|Compound:9044753|FD-Score:-3.47|P-value:1.60E-5 ID:SGTC_2987|Compound:9018338|FD-Score:4.25|P-value:1.72E-7 ID:SGTC_3142|Compound:9086498|FD-Score:-3.24|P-value:4.97E-5 ID:SGTC_3199|Compound:9111318|FD-Score:-2.76|P-value:4.60E-4 ID:SGTC_3231|Compound:9132313|FD-Score:-2.62|P-value:8.23E-4 ID:SGTC_3304|Compound:9125678|FD-Score:2.74|P-value:5.07E-4

Top fitness defect scores for YKL185W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2440 5628896 5.01 157.8 µM 9.64E-10 10 2.72E-7
2 SGTC_1718 roemerine 4.59 11.9 µM 1.93E-8 redox potentiating 28 2.24E-6
3 SGTC_373 0844-0104 4.41 25.9 µM 6.31E-8 ERAD & cell cycle 38 5.17E-6
4 SGTC_2987 9018338 4.25 71.4 µM 1.72E-7 RPP1 & pyrimidine depletion 9 1.05E-5
5 SGTC_1754 st042065 4.09 16.1 µM 4.91E-7 NEO1-PIK1 168 2.20E-5
6 SGTC_2442 5158906 3.53 48.5 µM 1.20E-5 33 2.11E-4
7 SGTC_2584 androsterone 3.52 48.7 µM 1.23E-5 cell wall 156 2.15E-4
8 SGTC_2686 alizarin 3.51 95.4 µM 1.28E-5 RPP1 & pyrimidine depletion 39 2.21E-4
9 SGTC_474 dl-dihydrosphingosine 3.51 5.0 µM 1.31E-5 42 2.24E-4
10 SGTC_2757 clomiphene 3.49 4.2 µM 1.41E-5 fatty acid desaturase (OLE1) 61 2.37E-4
11 SGTC_2036 5160441 3.49 105.0 µM 1.44E-5 NEO1 97 2.40E-4
12 SGTC_457 grayanotoxin iii 3.45 135.0 µM 1.75E-5 96 2.77E-4
13 SGTC_1132 3456-3614 3.25 2.5 µM 5.02E-5 tubulin folding & SWR complex 92 5.83E-4
14 SGTC_2761 levamisole 3.24 97.9 µM 5.12E-5 39 5.92E-4
15 SGTC_1312 1218-2183 3.20 165.0 µM 6.38E-5 19 6.92E-4
16 SGTC_65 0798-0905 3.15 35.0 µM 8.23E-5 calcium & mitochondrial duress 147 8.30E-4
17 SGTC_480 am 92016 3.10 50.0 µM 1.05E-4 47 9.84E-4
18 SGTC_475 α-linolenic acid 3.00 10.0 µM 1.62E-4 excess fatty acid 58 0.00134
19 SGTC_2808 7946468 2.93 45.5 µM 2.24E-4 82 0.00170
20 SGTC_2484 5809818 2.89 12.0 µM 2.62E-4 58 0.00190

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.269 1.16E-56 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress
0.268 3.26E-56 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.263 2.21E-54 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.253 4.14E-50 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.251 2.88E-49 YFL014W HSP12 hom Plasma membrane protein involved in maintaining membrane organization in stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
0.247 8.12E-48 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.247 1.32E-47 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.242 9.64E-46 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.238 2.44E-44 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.234 5.15E-43 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.228 6.46E-41 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.227 1.43E-40 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.227 2.78E-40 YCL026C-A FRM2 hom Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
0.224 1.51E-39 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.217 3.74E-37 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus