YKL194C / MST1

Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(XXX)Q2 in vitro

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL194C / MST1

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.88|P-value:6.24E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.16|P-value:1.44E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:4.22|P-value:2.20E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:8.60|P-value:3.89E-13 ID:SGTC_149|Compound:3970-0822|FD-Score:6.37|P-value:5.60E-8 ID:SGTC_150|Compound:3970-0969|FD-Score:3.88|P-value:6.08E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-7.82|P-value:3.68E-11 ID:SGTC_157|Compound:1348-1695|FD-Score:5.03|P-value:1.39E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.79|P-value:3.29E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:5.96|P-value:3.48E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:29.30|P-value:2.38E-131 ID:SGTC_275|Compound:trichlorophene|FD-Score:11.70|P-value:1.15E-22 ID:SGTC_310|Compound:1273-0058|FD-Score:4.05|P-value:3.75E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.46|P-value:1.03E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:36.90|P-value:3.54E-207 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.85|P-value:6.81E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.94|P-value:5.23E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-5.64|P-value:1.33E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:4.44|P-value:1.09E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.31|P-value:1.67E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-4.04|P-value:3.89E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:5.44|P-value:2.93E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:-4.65|P-value:5.39E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:-4.88|P-value:2.43E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:5.35|P-value:4.17E-6 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.28|P-value:1.85E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:7.74|P-value:5.69E-11 ID:SGTC_1635|Compound:st007035|FD-Score:29.00|P-value:2.12E-129 ID:SGTC_1658|Compound:st012944|FD-Score:-4.16|P-value:2.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-5.15|P-value:9.03E-6 ID:SGTC_1727|Compound:st034309|FD-Score:4.17|P-value:2.59E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-5.19|P-value:7.74E-6 ID:SGTC_1742|Compound:st038235|FD-Score:5.71|P-value:9.82E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-4.66|P-value:5.19E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.54|P-value:7.90E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.09|P-value:3.31E-4 ID:SGTC_1882|Compound:5615643|FD-Score:3.73|P-value:9.59E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.64|P-value:1.31E-6 ID:SGTC_2050|Compound:5263056|FD-Score:-4.26|P-value:1.96E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.05|P-value:3.75E-4 ID:SGTC_2113|Compound:5491891|FD-Score:4.29|P-value:1.75E-4 ID:SGTC_2369|Compound:9071472|FD-Score:6.55|P-value:2.50E-8 ID:SGTC_2472|Compound:5377652|FD-Score:4.96|P-value:1.79E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.01|P-value:4.23E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.02|P-value:4.06E-4 ID:SGTC_2693|Compound:st077581|FD-Score:4.83|P-value:2.89E-5 ID:SGTC_2702|Compound:st077768|FD-Score:3.93|P-value:5.38E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-5.04|P-value:1.37E-5 ID:SGTC_2810|Compound:7959871|FD-Score:-7.26|P-value:7.63E-10 ID:SGTC_2811|Compound:7967822|FD-Score:6.60|P-value:1.98E-8 ID:SGTC_2815|Compound:7968870|FD-Score:-5.42|P-value:3.18E-6 ID:SGTC_2836|Compound:9010338|FD-Score:-5.59|P-value:1.65E-6 ID:SGTC_2905|Compound:7799065|FD-Score:-4.07|P-value:3.49E-4 ID:SGTC_2960|Compound:9079389|FD-Score:4.31|P-value:1.65E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-4.82|P-value:3.04E-5 ID:SGTC_2980|Compound:9014342|FD-Score:8.32|P-value:2.17E-12 ID:SGTC_2986|Compound:9022445|FD-Score:-4.70|P-value:4.54E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.78|P-value:8.27E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.77|P-value:3.57E-5 ID:SGTC_3129|Compound:9127257|FD-Score:4.08|P-value:3.40E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.77|P-value:8.35E-4 ID:SGTC_3218|Compound:9131285|FD-Score:8.14|P-value:6.06E-12 ID:SGTC_3286|Compound:9117135|FD-Score:-3.75|P-value:9.06E-4 ID:SGTC_3332|Compound:9141069|FD-Score:3.77|P-value:8.34E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:3.88|P-value:6.24E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.16|P-value:1.44E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:4.22|P-value:2.20E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:8.60|P-value:3.89E-13 ID:SGTC_149|Compound:3970-0822|FD-Score:6.37|P-value:5.60E-8 ID:SGTC_150|Compound:3970-0969|FD-Score:3.88|P-value:6.08E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:-7.82|P-value:3.68E-11 ID:SGTC_157|Compound:1348-1695|FD-Score:5.03|P-value:1.39E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.79|P-value:3.29E-5 ID:SGTC_173|Compound:1273-0061|FD-Score:5.96|P-value:3.48E-7 ID:SGTC_236|Compound:1509-0011|FD-Score:29.30|P-value:2.38E-131 ID:SGTC_275|Compound:trichlorophene|FD-Score:11.70|P-value:1.15E-22 ID:SGTC_310|Compound:1273-0058|FD-Score:4.05|P-value:3.75E-4 ID:SGTC_356|Compound:1634-0012|FD-Score:4.46|P-value:1.03E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:36.90|P-value:3.54E-207 ID:SGTC_416|Compound:estradiol valerate|FD-Score:3.85|P-value:6.81E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.94|P-value:5.23E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:-5.64|P-value:1.33E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:4.44|P-value:1.09E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:-4.31|P-value:1.67E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:-4.04|P-value:3.89E-4 ID:SGTC_908|Compound:3448-7491|FD-Score:5.44|P-value:2.93E-6 ID:SGTC_930|Compound:2810-3379|FD-Score:-4.65|P-value:5.39E-5 ID:SGTC_932|Compound:2857-0803|FD-Score:-4.88|P-value:2.43E-5 ID:SGTC_1122|Compound:capsaicin|FD-Score:5.35|P-value:4.17E-6 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.28|P-value:1.85E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:7.74|P-value:5.69E-11 ID:SGTC_1635|Compound:st007035|FD-Score:29.00|P-value:2.12E-129 ID:SGTC_1658|Compound:st012944|FD-Score:-4.16|P-value:2.67E-4 ID:SGTC_1707|Compound:st032323|FD-Score:-5.15|P-value:9.03E-6 ID:SGTC_1727|Compound:st034309|FD-Score:4.17|P-value:2.59E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-5.19|P-value:7.74E-6 ID:SGTC_1742|Compound:st038235|FD-Score:5.71|P-value:9.82E-7 ID:SGTC_1754|Compound:st042065|FD-Score:-4.66|P-value:5.19E-5 ID:SGTC_1787|Compound:fluconazole|FD-Score:-4.54|P-value:7.90E-5 ID:SGTC_1800|Compound:st050079|FD-Score:-4.09|P-value:3.31E-4 ID:SGTC_1882|Compound:5615643|FD-Score:3.73|P-value:9.59E-4 ID:SGTC_1883|Compound:5-benzylidenerhodanine|FD-Score:5.64|P-value:1.31E-6 ID:SGTC_2050|Compound:5263056|FD-Score:-4.26|P-value:1.96E-4 ID:SGTC_2075|Compound:5212009|FD-Score:4.05|P-value:3.75E-4 ID:SGTC_2113|Compound:5491891|FD-Score:4.29|P-value:1.75E-4 ID:SGTC_2369|Compound:9071472|FD-Score:6.55|P-value:2.50E-8 ID:SGTC_2472|Compound:5377652|FD-Score:4.96|P-value:1.79E-5 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:4.01|P-value:4.23E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.02|P-value:4.06E-4 ID:SGTC_2693|Compound:st077581|FD-Score:4.83|P-value:2.89E-5 ID:SGTC_2702|Compound:st077768|FD-Score:3.93|P-value:5.38E-4 ID:SGTC_2804|Compound:7954240|FD-Score:-5.04|P-value:1.37E-5 ID:SGTC_2810|Compound:7959871|FD-Score:-7.26|P-value:7.63E-10 ID:SGTC_2811|Compound:7967822|FD-Score:6.60|P-value:1.98E-8 ID:SGTC_2815|Compound:7968870|FD-Score:-5.42|P-value:3.18E-6 ID:SGTC_2836|Compound:9010338|FD-Score:-5.59|P-value:1.65E-6 ID:SGTC_2905|Compound:7799065|FD-Score:-4.07|P-value:3.49E-4 ID:SGTC_2960|Compound:9079389|FD-Score:4.31|P-value:1.65E-4 ID:SGTC_2977|Compound:9011197|FD-Score:-4.82|P-value:3.04E-5 ID:SGTC_2980|Compound:9014342|FD-Score:8.32|P-value:2.17E-12 ID:SGTC_2986|Compound:9022445|FD-Score:-4.70|P-value:4.54E-5 ID:SGTC_2989|Compound:9073649|FD-Score:3.78|P-value:8.27E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-4.77|P-value:3.57E-5 ID:SGTC_3129|Compound:9127257|FD-Score:4.08|P-value:3.40E-4 ID:SGTC_3178|Compound:9107506|FD-Score:3.77|P-value:8.35E-4 ID:SGTC_3218|Compound:9131285|FD-Score:8.14|P-value:6.06E-12 ID:SGTC_3286|Compound:9117135|FD-Score:-3.75|P-value:9.06E-4 ID:SGTC_3332|Compound:9141069|FD-Score:3.77|P-value:8.34E-4

Top fitness defect scores for YKL194C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_386 1509-0011 36.90 3.1 µM 3.54E-207 2 8.35E-298
2 SGTC_236 1509-0011 29.30 5.3 µM 2.38E-131 17 1.88E-188
3 SGTC_1635 st007035 29.00 42.4 µM 2.12E-129 8 1.21E-185
4 SGTC_275 trichlorophene 11.70 5.7 µM 1.15E-22 mitochondrial stress 25 7.76E-32
5 SGTC_134 0269-0018 8.60 6.9 µM 3.89E-13 53 3.82E-18
6 SGTC_2980 9014342 8.32 25.3 µM 2.17E-12 8 4.49E-17
7 SGTC_3218 9131285 8.14 49.5 µM 6.06E-12 endomembrane recycling 8 1.96E-16
8 SGTC_1528 arabinosylhypoxanthine 7.74 74.6 µM 5.69E-11 3 4.86E-15
9 SGTC_2811 7967822 6.60 32.5 µM 1.98E-8 2 2.10E-11
10 SGTC_2369 9071472 6.55 200.0 µM 2.50E-8 1 2.94E-11
11 SGTC_149 3970-0822 6.37 46.1 µM 5.60E-8 tubulin folding & SWR complex 31 9.33E-11
12 SGTC_70 0141-0289 6.16 43.3 µM 1.44E-7 mitochondrial stress 77 3.60E-10
13 SGTC_173 1273-0061 5.96 2.7 µM 3.48E-7 mitochondrial stress 108 1.27E-9
14 SGTC_1742 st038235 5.71 28.9 µM 9.82E-7 mitochondrial stress 52 5.56E-9
15 SGTC_1883 5-benzylidenerhodanine 5.64 20.0 µM 1.31E-6 TRP & mitochondrial translation 21 8.43E-9
16 SGTC_908 3448-7491 5.44 47.7 µM 2.93E-6 mitochondrial stress 61 2.64E-8
17 SGTC_1122 capsaicin 5.35 818.6 nM 4.17E-6 8 4.37E-8
18 SGTC_157 1348-1695 5.03 65.8 µM 1.39E-5 iron homeostasis 33 2.42E-7
19 SGTC_2472 5377652 4.96 49.4 µM 1.79E-5 TRP & mitochondrial translation 32 3.48E-7
20 SGTC_2693 st077581 4.83 40.7 µM 2.89E-5 15 6.85E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.706 0 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.705 0 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.680 0 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.652 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.644 0 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.641 0 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.634 0 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.631 0 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.628 0 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.623 0 YDR231C COX20 hom Mitochondrial inner membrane protein, required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
0.621 0 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.619 0 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.616 0 YDR518W EUG1 hom Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
0.613 0 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.609 0 YNL213C RRG9 hom Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies