YKL195W / MIA40

Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL195W / MIA40

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:11.60|P-value:4.61E-23 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.62|P-value:9.72E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.68|P-value:9.26E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.59|P-value:1.06E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:4.00|P-value:3.55E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.65|P-value:4.06E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:5.61|P-value:1.00E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:4.87|P-value:1.87E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:5.22|P-value:4.89E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:8.35|P-value:6.80E-13 ID:SGTC_123|Compound:0083-0120|FD-Score:5.13|P-value:6.96E-6 ID:SGTC_124|Compound:0136-0217|FD-Score:7.16|P-value:6.24E-10 ID:SGTC_131|Compound:0141-0277|FD-Score:4.53|P-value:6.26E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:4.16|P-value:2.12E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.34|P-value:3.00E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:11.00|P-value:5.10E-21 ID:SGTC_188|Compound:3229-0966|FD-Score:5.21|P-value:5.09E-6 ID:SGTC_208|Compound:k072-0098|FD-Score:5.35|P-value:2.92E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:8.17|P-value:2.07E-12 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.68|P-value:7.28E-7 ID:SGTC_393|Compound:acivicin|FD-Score:4.10|P-value:2.62E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:5.28|P-value:3.79E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:5.44|P-value:2.01E-6 ID:SGTC_630|Compound:4356-0227|FD-Score:3.69|P-value:8.95E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:3.73|P-value:7.91E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:5.24|P-value:4.53E-6 ID:SGTC_818|Compound:4072-2707|FD-Score:3.79|P-value:6.59E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:6.12|P-value:1.03E-7 ID:SGTC_850|Compound:0388-0143|FD-Score:4.77|P-value:2.64E-5 ID:SGTC_853|Compound:0419-0306|FD-Score:4.01|P-value:3.46E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.66|P-value:3.88E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.15|P-value:6.31E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:5.01|P-value:1.10E-5 ID:SGTC_1098|Compound:clofazimine|FD-Score:5.31|P-value:3.44E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.31|P-value:3.34E-6 ID:SGTC_1131|Compound:3454-3128|FD-Score:7.75|P-value:2.34E-11 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.58|P-value:5.27E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.92|P-value:2.61E-7 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.73|P-value:3.11E-5 ID:SGTC_1453|Compound:4237-0541|FD-Score:5.11|P-value:7.36E-6 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.67|P-value:3.77E-5 ID:SGTC_1516|Compound:st013856|FD-Score:5.78|P-value:4.78E-7 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:4.76|P-value:2.70E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.14|P-value:2.28E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.64|P-value:4.21E-5 ID:SGTC_1781|Compound:st038725|FD-Score:3.84|P-value:5.78E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:5.15|P-value:6.37E-6 ID:SGTC_1831|Compound:st055328|FD-Score:8.24|P-value:1.32E-12 ID:SGTC_1898|Compound:st060199|FD-Score:3.71|P-value:8.49E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.81|P-value:6.24E-4 ID:SGTC_2066|Compound:5222451|FD-Score:4.22|P-value:1.76E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.37|P-value:1.08E-4 ID:SGTC_2225|Compound:6654152|FD-Score:10.40|P-value:6.11E-19 ID:SGTC_2233|Compound:sulbentine|FD-Score:7.12|P-value:7.70E-10 ID:SGTC_2336|Compound:9001819|FD-Score:8.45|P-value:3.68E-13 ID:SGTC_2344|Compound:7990960|FD-Score:7.90|P-value:1.03E-11 ID:SGTC_2430|Compound:5571206|FD-Score:7.79|P-value:1.86E-11 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.69|P-value:3.59E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:5.02|P-value:1.05E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.85|P-value:2.01E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.73|P-value:7.92E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.68|P-value:9.31E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.92|P-value:4.54E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.41|P-value:9.32E-5 ID:SGTC_2799|Compound:7812892|FD-Score:9.22|P-value:2.42E-15 ID:SGTC_2921|Compound:7974240|FD-Score:5.33|P-value:3.10E-6 ID:SGTC_2930|Compound:9013230|FD-Score:3.66|P-value:9.80E-4 ID:SGTC_2962|Compound:9082311|FD-Score:8.36|P-value:6.43E-13 ID:SGTC_2963|Compound:9082507|FD-Score:4.08|P-value:2.73E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.89|P-value:4.94E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.84|P-value:5.76E-4 ID:SGTC_3317|Compound:9138495|FD-Score:8.67|P-value:8.84E-14 ID:SGTC_16|Compound:erodoxin|FD-Score:11.60|P-value:4.61E-23 ID:SGTC_23|Compound:0213-0011|FD-Score:-3.62|P-value:9.72E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.68|P-value:9.26E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.59|P-value:1.06E-6 ID:SGTC_69|Compound:0096-0274|FD-Score:4.00|P-value:3.55E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.65|P-value:4.06E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:5.61|P-value:1.00E-6 ID:SGTC_115|Compound:0527-0192|FD-Score:4.87|P-value:1.87E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:5.22|P-value:4.89E-6 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:8.35|P-value:6.80E-13 ID:SGTC_123|Compound:0083-0120|FD-Score:5.13|P-value:6.96E-6 ID:SGTC_124|Compound:0136-0217|FD-Score:7.16|P-value:6.24E-10 ID:SGTC_131|Compound:0141-0277|FD-Score:4.53|P-value:6.26E-5 ID:SGTC_148|Compound:3253-0744|FD-Score:4.16|P-value:2.12E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:5.34|P-value:3.00E-6 ID:SGTC_177|Compound:1327-0068|FD-Score:11.00|P-value:5.10E-21 ID:SGTC_188|Compound:3229-0966|FD-Score:5.21|P-value:5.09E-6 ID:SGTC_208|Compound:k072-0098|FD-Score:5.35|P-value:2.92E-6 ID:SGTC_210|Compound:3551-0445|FD-Score:8.17|P-value:2.07E-12 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.68|P-value:7.28E-7 ID:SGTC_393|Compound:acivicin|FD-Score:4.10|P-value:2.62E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:5.28|P-value:3.79E-6 ID:SGTC_553|Compound:k007-0256|FD-Score:5.44|P-value:2.01E-6 ID:SGTC_630|Compound:4356-0227|FD-Score:3.69|P-value:8.95E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:3.73|P-value:7.91E-4 ID:SGTC_745|Compound:0469-0705|FD-Score:5.24|P-value:4.53E-6 ID:SGTC_818|Compound:4072-2707|FD-Score:3.79|P-value:6.59E-4 ID:SGTC_842|Compound:0271-0008|FD-Score:6.12|P-value:1.03E-7 ID:SGTC_850|Compound:0388-0143|FD-Score:4.77|P-value:2.64E-5 ID:SGTC_853|Compound:0419-0306|FD-Score:4.01|P-value:3.46E-4 ID:SGTC_966|Compound:1300-0360|FD-Score:4.66|P-value:3.88E-5 ID:SGTC_1044|Compound:1222-0021|FD-Score:5.15|P-value:6.31E-6 ID:SGTC_1057|Compound:3448-5381|FD-Score:5.01|P-value:1.10E-5 ID:SGTC_1098|Compound:clofazimine|FD-Score:5.31|P-value:3.44E-6 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.31|P-value:3.34E-6 ID:SGTC_1131|Compound:3454-3128|FD-Score:7.75|P-value:2.34E-11 ID:SGTC_1147|Compound:3809-1045|FD-Score:4.58|P-value:5.27E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:5.92|P-value:2.61E-7 ID:SGTC_1371|Compound:2421-0008|FD-Score:4.73|P-value:3.11E-5 ID:SGTC_1453|Compound:4237-0541|FD-Score:5.11|P-value:7.36E-6 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.67|P-value:3.77E-5 ID:SGTC_1516|Compound:st013856|FD-Score:5.78|P-value:4.78E-7 ID:SGTC_1552|Compound:6-methylflavone|FD-Score:4.76|P-value:2.70E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.14|P-value:2.28E-4 ID:SGTC_1765|Compound:st044815|FD-Score:4.64|P-value:4.21E-5 ID:SGTC_1781|Compound:st038725|FD-Score:3.84|P-value:5.78E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:5.15|P-value:6.37E-6 ID:SGTC_1831|Compound:st055328|FD-Score:8.24|P-value:1.32E-12 ID:SGTC_1898|Compound:st060199|FD-Score:3.71|P-value:8.49E-4 ID:SGTC_1925|Compound:st057770|FD-Score:3.81|P-value:6.24E-4 ID:SGTC_2066|Compound:5222451|FD-Score:4.22|P-value:1.76E-4 ID:SGTC_2124|Compound:5246320|FD-Score:4.37|P-value:1.08E-4 ID:SGTC_2225|Compound:6654152|FD-Score:10.40|P-value:6.11E-19 ID:SGTC_2233|Compound:sulbentine|FD-Score:7.12|P-value:7.70E-10 ID:SGTC_2336|Compound:9001819|FD-Score:8.45|P-value:3.68E-13 ID:SGTC_2344|Compound:7990960|FD-Score:7.90|P-value:1.03E-11 ID:SGTC_2430|Compound:5571206|FD-Score:7.79|P-value:1.86E-11 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:4.69|P-value:3.59E-5 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:5.02|P-value:1.05E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.85|P-value:2.01E-5 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.73|P-value:7.92E-4 ID:SGTC_2696|Compound:st077691|FD-Score:3.68|P-value:9.31E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.92|P-value:4.54E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.41|P-value:9.32E-5 ID:SGTC_2799|Compound:7812892|FD-Score:9.22|P-value:2.42E-15 ID:SGTC_2921|Compound:7974240|FD-Score:5.33|P-value:3.10E-6 ID:SGTC_2930|Compound:9013230|FD-Score:3.66|P-value:9.80E-4 ID:SGTC_2962|Compound:9082311|FD-Score:8.36|P-value:6.43E-13 ID:SGTC_2963|Compound:9082507|FD-Score:4.08|P-value:2.73E-4 ID:SGTC_2968|Compound:9090663|FD-Score:3.89|P-value:4.94E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.84|P-value:5.76E-4 ID:SGTC_3317|Compound:9138495|FD-Score:8.67|P-value:8.84E-14

Top fitness defect scores for YKL195W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_16 erodoxin 11.60 22.8 µM 4.61E-23 unfolded protein response 3 3.31E-31 1.52
2 SGTC_177 1327-0068 11.00 8.4 µM 5.10E-21 4 2.18E-28 2.25
3 SGTC_2225 6654152 10.40 15.4 µM 6.11E-19 9 1.60E-25 1.16
4 SGTC_2799 7812892 9.22 81.8 µM 2.42E-15 superoxide 1 1.45E-20 0.93
5 SGTC_3317 9138495 8.67 8.8 µM 8.84E-14 3 2.07E-18 1.63
6 SGTC_2336 9001819 8.45 198.7 µM 3.68E-13 heme biosynthesis & mitochondrial translocase 14 1.48E-17 0.51
7 SGTC_2962 9082311 8.36 13.2 µM 6.43E-13 1 3.18E-17 1.29
8 SGTC_120 rhodamine 6G 8.35 52.3 µM 6.80E-13 mitochondrial processes 2 3.44E-17 1.31
9 SGTC_1831 st055328 8.24 12.0 µM 1.32E-12 heme biosynthesis & mitochondrial translocase 3 8.61E-17 0.87
10 SGTC_210 3551-0445 8.17 56.8 µM 2.07E-12 3 1.59E-16 0.29
11 SGTC_2344 7990960 7.90 77.0 µM 1.03E-11 12 1.44E-15 0.62
12 SGTC_2430 5571206 7.79 200.0 µM 1.86E-11 4 3.26E-15 0.13
13 SGTC_1131 3454-3128 7.75 32.3 µM 2.34E-11 1 4.50E-15 1.04
14 SGTC_124 0136-0217 7.16 7.1 µM 6.24E-10 mitochondrial stress 4 4.11E-13 0.32
15 SGTC_2233 sulbentine 7.12 40.4 µM 7.70E-10 20 5.48E-13 0.77
16 SGTC_842 0271-0008 6.12 11.1 µM 1.03E-7 heme biosynthesis & mitochondrial translocase 6 4.60E-10 0.79
17 SGTC_1310 1188-0297 5.92 994.0 nM 2.61E-7 azole & statin 7 1.64E-9 0.58
18 SGTC_1516 st013856 5.78 110.0 µM 4.78E-7 2 3.76E-9 1.43
19 SGTC_391 gliotoxin 5.68 8.3 µM 7.28E-7 1 6.70E-9 1.56
20 SGTC_87 0100-0052 5.61 35.5 µM 1.00E-6 9 1.04E-8 0.48

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.438 2.06E-157 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.357 3.26E-101 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.333 2.44E-87 YEL034W HYP2 het Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.304 1.30E-72 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.280 2.76E-61 YLR355C ILV5 het Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
0.274 1.04E-58 YBL105C PKC1 het Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.267 6.43E-56 YGR029W ERV1 het Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
0.264 2.93E-54 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.251 1.92E-49 YML091C RPM2 het Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
0.239 1.27E-44 YJL031C BET4 het Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.233 1.69E-42 YJR049C UTR1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.209 1.68E-34 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.205 3.27E-33 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.205 4.03E-33 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.204 7.78E-33 YKR008W RSC4 het Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p