HIPHOP chemogenomics database

YKL195W / MIA40

Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p

Zygosity: Heterozygous strain

fixedexpanded
Profile for YKL195W / MIA40

Click on Significant Values for Screen Details

Top fitness defect scores for YKL195W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_16	erodoxin	11.60	22.8 µM	4.61E-23	unfolded protein response	3	3.31E-31	1.52
2	SGTC_177	1327-0068	11.00	8.4 µM	5.10E-21		4	2.18E-28	2.25
3	SGTC_2225	6654152	10.40	15.4 µM	6.11E-19		9	1.60E-25	1.16
4	SGTC_2799	7812892	9.22	81.8 µM	2.42E-15	superoxide	1	1.45E-20	0.93
5	SGTC_3317	9138495	8.67	8.8 µM	8.84E-14		3	2.07E-18	1.63
6	SGTC_2336	9001819	8.45	198.7 µM	3.68E-13	heme biosynthesis & mitochondrial translocase	14	1.48E-17	0.51
7	SGTC_2962	9082311	8.36	13.2 µM	6.43E-13		1	3.18E-17	1.29
8	SGTC_120	rhodamine 6G	8.35	52.3 µM	6.80E-13	mitochondrial processes	2	3.44E-17	1.31
9	SGTC_1831	st055328	8.24	12.0 µM	1.32E-12	heme biosynthesis & mitochondrial translocase	3	8.61E-17	0.87
10	SGTC_210	3551-0445	8.17	56.8 µM	2.07E-12		3	1.59E-16	0.29
11	SGTC_2344	7990960	7.90	77.0 µM	1.03E-11		12	1.44E-15	0.62
12	SGTC_2430	5571206	7.79	200.0 µM	1.86E-11		4	3.26E-15	0.13
13	SGTC_1131	3454-3128	7.75	32.3 µM	2.34E-11		1	4.50E-15	1.04
14	SGTC_124	0136-0217	7.16	7.1 µM	6.24E-10	mitochondrial stress	4	4.11E-13	0.32
15	SGTC_2233	sulbentine	7.12	40.4 µM	7.70E-10		20	5.48E-13	0.77
16	SGTC_842	0271-0008	6.12	11.1 µM	1.03E-7	heme biosynthesis & mitochondrial translocase	6	4.60E-10	0.79
17	SGTC_1310	1188-0297	5.92	994.0 nM	2.61E-7	azole & statin	7	1.64E-9	0.58
18	SGTC_1516	st013856	5.78	110.0 µM	4.78E-7		2	3.76E-9	1.43
19	SGTC_391	gliotoxin	5.68	8.3 µM	7.28E-7		1	6.70E-9	1.56
20	SGTC_87	0100-0052	5.61	35.5 µM	1.00E-6		9	1.04E-8	0.48

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.438	2.06E-157	YBR192W	RIM2	het	Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.357	3.26E-101	YDL205C	HEM3	het	Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.333	2.44E-87	YEL034W	HYP2	het	Translation elongation factor eIF-5A; may function in translation initiation; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has a paralog, ANB1, that arose from the whole genome duplication
0.304	1.30E-72	YDR047W	HEM12	het	Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.280	2.76E-61	YLR355C	ILV5	het	Bifunctional acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
0.274	1.04E-58	YBL105C	PKC1	het	Protein serine/threonine kinase essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
0.267	6.43E-56	YGR029W	ERV1	het	Flavin-linked sulfhydryl oxidase of the mitochondrial intermembrane space (IMS), oxidizes Mia40p as part of a disulfide relay system that promotes IMS retention of imported proteins; ortholog of human hepatopoietin (ALR)
0.264	2.93E-54	YMR203W	TOM40	het	Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.251	1.92E-49	YML091C	RPM2	het	Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
0.239	1.27E-44	YJL031C	BET4	het	Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.233	1.69E-42	YJR049C	UTR1	hom	ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
0.209	1.68E-34	YER021W	RPN3	het	Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.205	3.27E-33	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.205	4.03E-33	YLR033W	RSC58	het	Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.204	7.78E-33	YKR008W	RSC4	het	Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p