YKL201C / MNN4

Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; expression increases in late-logarithmic and stationary growth phases; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL201C / MNN4

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:4.82|P-value:4.63E-7 ID:SGTC_614|Compound:0462-0418|FD-Score:-8.69|P-value:4.65E-19 ID:SGTC_801|Compound:0866-0028|FD-Score:-4.37|P-value:4.36E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.39|P-value:2.80E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.04|P-value:9.80E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.20|P-value:9.47E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.06|P-value:8.97E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.11|P-value:7.76E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.14|P-value:6.80E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.94|P-value:3.10E-5 ID:SGTC_2237|Compound:6667747|FD-Score:-4.20|P-value:9.54E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-6.19|P-value:1.49E-10 ID:SGTC_2244|Compound:6635614|FD-Score:3.87|P-value:4.02E-5 ID:SGTC_2262|Compound:9014885|FD-Score:-3.32|P-value:3.63E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.95|P-value:2.82E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-4.34|P-value:5.09E-6 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.04|P-value:9.87E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.05|P-value:9.53E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.04|P-value:9.64E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.02|P-value:2.11E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.19|P-value:5.84E-4 ID:SGTC_2924|Compound:9010168|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.75|P-value:6.62E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.15|P-value:1.20E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.19|P-value:5.86E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.32|P-value:5.30E-6 ID:SGTC_3256|Compound:9138902|FD-Score:3.04|P-value:9.77E-4 ID:SGTC_3327|Compound:9140648|FD-Score:-3.58|P-value:1.34E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:4.82|P-value:4.63E-7 ID:SGTC_614|Compound:0462-0418|FD-Score:-8.69|P-value:4.65E-19 ID:SGTC_801|Compound:0866-0028|FD-Score:-4.37|P-value:4.36E-6 ID:SGTC_1059|Compound:3448-6867|FD-Score:3.39|P-value:2.80E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.04|P-value:9.80E-4 ID:SGTC_1378|Compound:2093-0007|FD-Score:4.20|P-value:9.47E-6 ID:SGTC_1730|Compound:st033808|FD-Score:3.06|P-value:8.97E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.11|P-value:7.76E-4 ID:SGTC_1795|Compound:st049545|FD-Score:3.14|P-value:6.80E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.94|P-value:3.10E-5 ID:SGTC_2237|Compound:6667747|FD-Score:-4.20|P-value:9.54E-6 ID:SGTC_2239|Compound:6685329|FD-Score:-6.19|P-value:1.49E-10 ID:SGTC_2244|Compound:6635614|FD-Score:3.87|P-value:4.02E-5 ID:SGTC_2262|Compound:9014885|FD-Score:-3.32|P-value:3.63E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.95|P-value:2.82E-5 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-4.34|P-value:5.09E-6 ID:SGTC_2545|Compound:solidagenone|FD-Score:-3.04|P-value:9.87E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:3.05|P-value:9.53E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.04|P-value:9.64E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.02|P-value:2.11E-5 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.19|P-value:5.84E-4 ID:SGTC_2924|Compound:9010168|FD-Score:3.64|P-value:1.06E-4 ID:SGTC_2961|Compound:9083290|FD-Score:3.75|P-value:6.62E-5 ID:SGTC_3206|Compound:9114350|FD-Score:4.15|P-value:1.20E-5 ID:SGTC_3209|Compound:9113616|FD-Score:3.19|P-value:5.86E-4 ID:SGTC_3212|Compound:9128307|FD-Score:4.32|P-value:5.30E-6 ID:SGTC_3256|Compound:9138902|FD-Score:3.04|P-value:9.77E-4 ID:SGTC_3327|Compound:9140648|FD-Score:-3.58|P-value:1.34E-4

Top fitness defect scores for YKL201C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_6 0844-0013 4.82 19.8 µM 4.63E-7 45 7.30E-7
2 SGTC_3212 9128307 4.32 49.5 µM 5.30E-6 34 7.69E-6
3 SGTC_1378 2093-0007 4.20 380.0 nM 9.47E-6 19 1.35E-5
4 SGTC_3206 9114350 4.15 49.5 µM 1.20E-5 13 1.70E-5
5 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.95 3.7 µM 2.82E-5 84 3.85E-5
6 SGTC_2244 6635614 3.87 200.0 µM 4.02E-5 5 5.43E-5
7 SGTC_2961 9083290 3.75 32.5 µM 6.62E-5 23 8.80E-5
8 SGTC_2924 9010168 3.64 62.8 µM 1.06E-4 cell wall signaling 50 1.39E-4
9 SGTC_1059 3448-6867 3.39 171.0 µM 2.80E-4 25 3.54E-4
10 SGTC_3209 9113616 3.19 49.5 µM 5.86E-4 65 7.23E-4
11 SGTC_1795 st049545 3.14 62.3 µM 6.80E-4 TSC3-RPN4 33 8.34E-4
12 SGTC_1783 st039459 3.11 87.5 µM 7.76E-4 Golgi 53 9.49E-4
13 SGTC_1730 st033808 3.06 45.1 µM 8.97E-4 93 0.00109
14 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 3.05 100.0 µM 9.53E-4 71 0.00116
15 SGTC_2687 chenodiol 3.04 100.0 µM 9.64E-4 66 0.00117
16 SGTC_3256 9138902 3.04 49.5 µM 9.77E-4 PDR1 8 0.00119
17 SGTC_1061 3448-9325 3.04 110.0 µM 9.80E-4 54 0.00119
18 SGTC_2282 7944268 2.98 200.0 µM 0.00119 RPP1 & pyrimidine depletion 89 0.00144
19 SGTC_1831 st055328 2.93 12.0 µM 0.00144 heme biosynthesis & mitochondrial translocase 74 0.00172
20 SGTC_1141 3253-0389 2.88 52.6 µM 0.00169 39 0.00202

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.219 1.18E-37 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.208 2.94E-34 YMR019W STB4 hom Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
0.177 3.90E-25 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.177 4.18E-25 YLR334C_d YLR334C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here
0.172 9.46E-24 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.163 2.90E-21 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.154 2.63E-19 YKL171W NNK1 hom Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
0.150 2.02E-18 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.142 1.80E-16 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.142 1.94E-16 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.135 4.30E-15 YOR093C_p YOR093C_p hom Putative protein of unknown function; deletion causes sensitivity to unfolded protein response-inducing agents
0.132 1.98E-14 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.131 2.36E-14 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.131 2.40E-14 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.131 2.54E-14 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS