YKL204W / EAP1

eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL204W / EAP1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.81|P-value:7.71E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.90|P-value:5.36E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:4.28|P-value:6.05E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.51|P-value:8.32E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:4.12|P-value:1.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.84|P-value:2.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.41|P-value:3.75E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:-4.65|P-value:1.27E-5 ID:SGTC_694|Compound:1315-0438|FD-Score:3.51|P-value:8.33E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.56|P-value:7.03E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.15|P-value:1.30E-8 ID:SGTC_927|Compound:2425-1185|FD-Score:4.76|P-value:9.49E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:-4.14|P-value:8.72E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.68|P-value:4.25E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-5.67|P-value:1.41E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:-7.54|P-value:4.57E-12 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.64|P-value:4.88E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.11|P-value:1.15E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-5.28|P-value:8.79E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:-4.52|P-value:2.12E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:5.72|P-value:1.37E-7 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.65|P-value:5.30E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.52|P-value:8.07E-4 ID:SGTC_1664|Compound:st014217|FD-Score:4.55|P-value:2.24E-5 ID:SGTC_1674|Compound:st016616|FD-Score:3.49|P-value:8.84E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.94|P-value:2.02E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.45|P-value:9.79E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.71|P-value:1.17E-5 ID:SGTC_1895|Compound:st058434|FD-Score:4.20|P-value:8.15E-5 ID:SGTC_1906|Compound:st060206|FD-Score:4.52|P-value:2.50E-5 ID:SGTC_1909|Compound:st060837|FD-Score:3.61|P-value:6.10E-4 ID:SGTC_1924|Compound:st058459|FD-Score:-3.45|P-value:8.78E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.60|P-value:5.49E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.51|P-value:8.36E-4 ID:SGTC_2289|Compound:7946404|FD-Score:4.78|P-value:8.95E-6 ID:SGTC_2292|Compound:7671323|FD-Score:4.10|P-value:1.16E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.95|P-value:3.66E-6 ID:SGTC_2486|Compound:5283202|FD-Score:3.68|P-value:4.78E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.67|P-value:4.99E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:5.28|P-value:1.07E-6 ID:SGTC_2720|Compound:fluspirilene|FD-Score:4.23|P-value:7.28E-5 ID:SGTC_2740|Compound:felodipine|FD-Score:-5.36|P-value:6.07E-7 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-4.32|P-value:4.46E-5 ID:SGTC_3041|Compound:9091929|FD-Score:5.09|P-value:2.37E-6 ID:SGTC_3105|Compound:9121040|FD-Score:-4.53|P-value:2.06E-5 ID:SGTC_3138|Compound:9097216|FD-Score:4.54|P-value:2.30E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:4.81|P-value:7.71E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.90|P-value:5.36E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:4.28|P-value:6.05E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.51|P-value:8.32E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:4.12|P-value:1.09E-4 ID:SGTC_365|Compound:0121-0052|FD-Score:-3.84|P-value:2.49E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:4.41|P-value:3.75E-5 ID:SGTC_683|Compound:panaxydol|FD-Score:-4.65|P-value:1.27E-5 ID:SGTC_694|Compound:1315-0438|FD-Score:3.51|P-value:8.33E-4 ID:SGTC_776|Compound:4476-4378|FD-Score:3.56|P-value:7.03E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-6.15|P-value:1.30E-8 ID:SGTC_927|Compound:2425-1185|FD-Score:4.76|P-value:9.49E-6 ID:SGTC_934|Compound:2950-0684|FD-Score:-4.14|P-value:8.72E-5 ID:SGTC_951|Compound:1171-0579|FD-Score:-3.68|P-value:4.25E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-5.67|P-value:1.41E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:-7.54|P-value:4.57E-12 ID:SGTC_1126|Compound:isoteolin|FD-Score:-3.64|P-value:4.88E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:4.11|P-value:1.15E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-5.28|P-value:8.79E-7 ID:SGTC_1259|Compound:0671-0132|FD-Score:-4.52|P-value:2.12E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:5.72|P-value:1.37E-7 ID:SGTC_1384|Compound:3089-0840|FD-Score:3.65|P-value:5.30E-4 ID:SGTC_1534|Compound:6-azauridine|FD-Score:3.52|P-value:8.07E-4 ID:SGTC_1664|Compound:st014217|FD-Score:4.55|P-value:2.24E-5 ID:SGTC_1674|Compound:st016616|FD-Score:3.49|P-value:8.84E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.94|P-value:2.02E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.45|P-value:9.79E-4 ID:SGTC_1724|Compound:st037281|FD-Score:4.71|P-value:1.17E-5 ID:SGTC_1895|Compound:st058434|FD-Score:4.20|P-value:8.15E-5 ID:SGTC_1906|Compound:st060206|FD-Score:4.52|P-value:2.50E-5 ID:SGTC_1909|Compound:st060837|FD-Score:3.61|P-value:6.10E-4 ID:SGTC_1924|Compound:st058459|FD-Score:-3.45|P-value:8.78E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-3.60|P-value:5.49E-4 ID:SGTC_2225|Compound:6654152|FD-Score:3.51|P-value:8.36E-4 ID:SGTC_2289|Compound:7946404|FD-Score:4.78|P-value:8.95E-6 ID:SGTC_2292|Compound:7671323|FD-Score:4.10|P-value:1.16E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-4.95|P-value:3.66E-6 ID:SGTC_2486|Compound:5283202|FD-Score:3.68|P-value:4.78E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:3.67|P-value:4.99E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:5.28|P-value:1.07E-6 ID:SGTC_2720|Compound:fluspirilene|FD-Score:4.23|P-value:7.28E-5 ID:SGTC_2740|Compound:felodipine|FD-Score:-5.36|P-value:6.07E-7 ID:SGTC_2770|Compound:toltrazuril|FD-Score:-4.32|P-value:4.46E-5 ID:SGTC_3041|Compound:9091929|FD-Score:5.09|P-value:2.37E-6 ID:SGTC_3105|Compound:9121040|FD-Score:-4.53|P-value:2.06E-5 ID:SGTC_3138|Compound:9097216|FD-Score:4.54|P-value:2.30E-5

Top fitness defect scores for YKL204W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1370 2001-0092 5.72 44.6 µM 1.37E-7 8 5.29E-9
2 SGTC_2595 hexamethylquercetagetin 5.28 100.0 µM 1.07E-6 9 6.55E-8
3 SGTC_3041 9091929 5.09 49.5 µM 2.37E-6 redox potentiating 9 1.75E-7
4 SGTC_138 0850-0259 4.90 40.9 µM 5.36E-6 cell wall 145 4.73E-7
5 SGTC_58 0957-0393 4.81 9.0 µM 7.71E-6 cell wall 303 7.39E-7
6 SGTC_2289 7946404 4.78 165.1 µM 8.95E-6 64 8.87E-7
7 SGTC_927 2425-1185 4.76 123.0 µM 9.49E-6 12 9.53E-7
8 SGTC_1724 st037281 4.71 8.3 µM 1.17E-5 23 1.23E-6
9 SGTC_1664 st014217 4.55 9.0 µM 2.24E-5 60S ribosome export 6 2.72E-6
10 SGTC_3138 9097216 4.54 49.5 µM 2.30E-5 9 2.82E-6
11 SGTC_1906 st060206 4.52 94.7 µM 2.50E-5 12 3.11E-6
12 SGTC_568 1193-0097 4.41 7.5 µM 3.75E-5 ERAD & cell cycle 119 5.12E-6
13 SGTC_310 1273-0058 4.28 1.1 µM 6.05E-5 mitochondrial stress 225 9.20E-6
14 SGTC_2720 fluspirilene 4.23 36.9 µM 7.28E-5 ERG2 9 1.15E-5
15 SGTC_1895 st058434 4.20 18.3 µM 8.15E-5 14 1.32E-5
16 SGTC_358 0075-0058 4.12 9.4 µM 1.09E-4 54 1.90E-5
17 SGTC_1156 4092-0367 4.11 4.9 µM 1.15E-4 17 2.01E-5
18 SGTC_2292 7671323 4.10 168.1 µM 1.16E-4 RNA processing & uracil transport 28 2.03E-5
19 SGTC_1691 st019933 3.94 84.6 µM 2.02E-4 13 4.02E-5
20 SGTC_2486 5283202 3.68 22.4 µM 4.78E-4 42 1.15E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.71E-14 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.129 5.16E-14 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.118 7.75E-12 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.118 8.16E-12 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.115 2.76E-11 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.114 3.12E-11 YNL016W PUB1 hom Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds mRNA and is required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; protein abundance increases in response to DNA replication stress
0.114 4.13E-11 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.110 1.64E-10 YNL225C CNM67 hom Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
0.106 6.24E-10 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.106 6.96E-10 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.105 1.16E-9 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.103 1.92E-9 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.103 2.36E-9 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.102 2.62E-9 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.102 2.74E-9 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication