YKL208W / CBT1

Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL208W / CBT1

Click on Significant Values for Screen Details ID:SGTC_26|Compound:0302-0163|FD-Score:-4.44|P-value:3.99E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:15.80|P-value:2.44E-57 ID:SGTC_386|Compound:1509-0011|FD-Score:5.69|P-value:5.28E-9 ID:SGTC_427|Compound:1354-0053|FD-Score:3.79|P-value:6.94E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.26|P-value:5.18E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:4.53|P-value:2.58E-6 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.02|P-value:2.63E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.53|P-value:1.88E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.28|P-value:4.89E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:-3.68|P-value:1.05E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.23|P-value:5.70E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:6.90|P-value:1.90E-12 ID:SGTC_935|Compound:2996-0153|FD-Score:4.98|P-value:2.76E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:5.05|P-value:1.91E-7 ID:SGTC_964|Compound:1287-0107|FD-Score:4.43|P-value:4.19E-6 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.47|P-value:2.38E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.59|P-value:1.50E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.60|P-value:1.86E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.14|P-value:7.81E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.98|P-value:3.11E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.20|P-value:6.36E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.09|P-value:9.47E-4 ID:SGTC_1635|Compound:st007035|FD-Score:9.38|P-value:1.81E-21 ID:SGTC_1714|Compound:st033236|FD-Score:3.23|P-value:5.82E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-4.24|P-value:1.01E-5 ID:SGTC_1850|Compound:st056238|FD-Score:-3.48|P-value:2.34E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.86|P-value:5.08E-5 ID:SGTC_2020|Compound:4017018|FD-Score:3.27|P-value:5.02E-4 ID:SGTC_2681|Compound:menadione|FD-Score:-3.58|P-value:1.57E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.47|P-value:2.41E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.51|P-value:2.04E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.57|P-value:1.63E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.35|P-value:3.80E-4 ID:SGTC_2905|Compound:7799065|FD-Score:3.47|P-value:2.39E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-3.79|P-value:6.88E-5 ID:SGTC_2952|Compound:9073731|FD-Score:-3.53|P-value:1.89E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.60|P-value:1.88E-6 ID:SGTC_3071|Compound:9117417|FD-Score:3.18|P-value:6.80E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-4.29|P-value:7.93E-6 ID:SGTC_3281|Compound:9140613|FD-Score:-4.58|P-value:2.03E-6 ID:SGTC_3290|Compound:9121031|FD-Score:3.07|P-value:9.90E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.94|P-value:3.70E-5 ID:SGTC_26|Compound:0302-0163|FD-Score:-4.44|P-value:3.99E-6 ID:SGTC_236|Compound:1509-0011|FD-Score:15.80|P-value:2.44E-57 ID:SGTC_386|Compound:1509-0011|FD-Score:5.69|P-value:5.28E-9 ID:SGTC_427|Compound:1354-0053|FD-Score:3.79|P-value:6.94E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.26|P-value:5.18E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:4.53|P-value:2.58E-6 ID:SGTC_742|Compound:0kpi-0133|FD-Score:4.02|P-value:2.63E-5 ID:SGTC_744|Compound:1594-0068|FD-Score:3.53|P-value:1.88E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:-3.28|P-value:4.89E-4 ID:SGTC_856|Compound:0433-0016|FD-Score:-3.68|P-value:1.05E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.23|P-value:5.70E-4 ID:SGTC_931|Compound:2812-0076|FD-Score:6.90|P-value:1.90E-12 ID:SGTC_935|Compound:2996-0153|FD-Score:4.98|P-value:2.76E-7 ID:SGTC_936|Compound:3005-4620|FD-Score:5.05|P-value:1.91E-7 ID:SGTC_964|Compound:1287-0107|FD-Score:4.43|P-value:4.19E-6 ID:SGTC_1114|Compound:scopoletin|FD-Score:3.47|P-value:2.38E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:3.59|P-value:1.50E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:4.60|P-value:1.86E-6 ID:SGTC_1375|Compound:2910-0797|FD-Score:-3.14|P-value:7.81E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.98|P-value:3.11E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.20|P-value:6.36E-4 ID:SGTC_1503|Compound:4585-0012|FD-Score:3.09|P-value:9.47E-4 ID:SGTC_1635|Compound:st007035|FD-Score:9.38|P-value:1.81E-21 ID:SGTC_1714|Compound:st033236|FD-Score:3.23|P-value:5.82E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-4.24|P-value:1.01E-5 ID:SGTC_1850|Compound:st056238|FD-Score:-3.48|P-value:2.34E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.86|P-value:5.08E-5 ID:SGTC_2020|Compound:4017018|FD-Score:3.27|P-value:5.02E-4 ID:SGTC_2681|Compound:menadione|FD-Score:-3.58|P-value:1.57E-4 ID:SGTC_2700|Compound:st077718|FD-Score:3.47|P-value:2.41E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.51|P-value:2.04E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:3.57|P-value:1.63E-4 ID:SGTC_2832|Compound:7992762|FD-Score:-3.35|P-value:3.80E-4 ID:SGTC_2905|Compound:7799065|FD-Score:3.47|P-value:2.39E-4 ID:SGTC_2927|Compound:9011802|FD-Score:-3.79|P-value:6.88E-5 ID:SGTC_2952|Compound:9073731|FD-Score:-3.53|P-value:1.89E-4 ID:SGTC_2971|Compound:9092186|FD-Score:4.60|P-value:1.88E-6 ID:SGTC_3071|Compound:9117417|FD-Score:3.18|P-value:6.80E-4 ID:SGTC_3270|Compound:9137563|FD-Score:-4.29|P-value:7.93E-6 ID:SGTC_3281|Compound:9140613|FD-Score:-4.58|P-value:2.03E-6 ID:SGTC_3290|Compound:9121031|FD-Score:3.07|P-value:9.90E-4 ID:SGTC_3299|Compound:9121982|FD-Score:3.94|P-value:3.70E-5

Top fitness defect scores for YKL208W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 15.80 5.3 µM 2.44E-57 88 1.19E-56
2 SGTC_1635 st007035 9.38 42.4 µM 1.81E-21 81 3.16E-21
3 SGTC_931 2812-0076 6.90 566.0 µM 1.90E-12 1 2.57E-12
4 SGTC_386 1509-0011 5.69 3.1 µM 5.28E-9 94 6.50E-9
5 SGTC_936 3005-4620 5.05 401.0 µM 1.91E-7 9 2.25E-7
6 SGTC_935 2996-0153 4.98 430.0 µM 2.76E-7 PDR1 8 3.23E-7
7 SGTC_1263 0774-1208 4.60 64.8 µM 1.86E-6 30 2.14E-6
8 SGTC_2971 9092186 4.60 9.9 µM 1.88E-6 ergosterol biosynthesis 18 2.15E-6
9 SGTC_607 4491-0986 4.53 66.8 µM 2.58E-6 heme requiring 14 2.95E-6
10 SGTC_964 1287-0107 4.43 19.4 µM 4.19E-6 cell wall 27 4.76E-6
11 SGTC_742 0kpi-0133 4.02 140.0 µM 2.63E-5 ERG2 22 2.92E-5
12 SGTC_1424 4048-1941 3.98 275.0 µM 3.11E-5 24 3.44E-5
13 SGTC_3299 9121982 3.94 43.1 µM 3.70E-5 29 4.09E-5
14 SGTC_2002 4023503 3.86 131.0 µM 5.08E-5 mitochondrial processes 34 5.60E-5
15 SGTC_427 1354-0053 3.79 47.3 µM 6.94E-5 27 7.62E-5
16 SGTC_1134 3477-0009 3.59 42.5 µM 1.50E-4 57 1.63E-4
17 SGTC_2721 ifenprodil 3.57 61.4 µM 1.63E-4 plasma membrane duress 31 1.78E-4
18 SGTC_744 1594-0068 3.53 283.0 µM 1.88E-4 amide catabolism 41 2.04E-4
19 SGTC_1114 scopoletin 3.47 1.7 µM 2.38E-4 30 2.58E-4
20 SGTC_2905 7799065 3.47 20.1 µM 2.39E-4 24 2.59E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.567 1.39E-284 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.543 9.72E-256 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.505 1.90E-216 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.502 3.69E-213 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.501 1.44E-212 YHR050W-A_p YHR050W-A_p hom Protein of unknown function; identified by expression profiling and mass spectrometry
0.499 3.50E-210 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.495 1.96E-206 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.493 1.82E-204 YMR035W IMP2 hom Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.489 1.39E-200 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.488 5.86E-200 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.488 1.50E-199 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.487 5.86E-199 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.471 1.94E-184 YOR065W CYT1 hom Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.467 2.85E-181 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.467 4.61E-181 YGL237C HAP2 hom Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding