HIPHOP chemogenomics database

YKL208W / CBT1

Protein involved in 5' end processing of mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p

Zygosity: Homozygous strain

fixedexpanded
Profile for YKL208W / CBT1

Click on Significant Values for Screen Details

Top fitness defect scores for YKL208W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_236	1509-0011	15.80	5.3 µM	2.44E-57		88	1.19E-56
2	SGTC_1635	st007035	9.38	42.4 µM	1.81E-21		81	3.16E-21
3	SGTC_931	2812-0076	6.90	566.0 µM	1.90E-12		1	2.57E-12
4	SGTC_386	1509-0011	5.69	3.1 µM	5.28E-9		94	6.50E-9
5	SGTC_936	3005-4620	5.05	401.0 µM	1.91E-7		9	2.25E-7
6	SGTC_935	2996-0153	4.98	430.0 µM	2.76E-7	PDR1	8	3.23E-7
7	SGTC_1263	0774-1208	4.60	64.8 µM	1.86E-6		30	2.14E-6
8	SGTC_2971	9092186	4.60	9.9 µM	1.88E-6	ergosterol biosynthesis	18	2.15E-6
9	SGTC_607	4491-0986	4.53	66.8 µM	2.58E-6	heme requiring	14	2.95E-6
10	SGTC_964	1287-0107	4.43	19.4 µM	4.19E-6	cell wall	27	4.76E-6
11	SGTC_742	0kpi-0133	4.02	140.0 µM	2.63E-5	ERG2	22	2.92E-5
12	SGTC_1424	4048-1941	3.98	275.0 µM	3.11E-5		24	3.44E-5
13	SGTC_3299	9121982	3.94	43.1 µM	3.70E-5		29	4.09E-5
14	SGTC_2002	4023503	3.86	131.0 µM	5.08E-5	mitochondrial processes	34	5.60E-5
15	SGTC_427	1354-0053	3.79	47.3 µM	6.94E-5		27	7.62E-5
16	SGTC_1134	3477-0009	3.59	42.5 µM	1.50E-4		57	1.63E-4
17	SGTC_2721	ifenprodil	3.57	61.4 µM	1.63E-4	plasma membrane duress	31	1.78E-4
18	SGTC_744	1594-0068	3.53	283.0 µM	1.88E-4	amide catabolism	41	2.04E-4
19	SGTC_1114	scopoletin	3.47	1.7 µM	2.38E-4		30	2.58E-4
20	SGTC_2905	7799065	3.47	20.1 µM	2.39E-4		24	2.59E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.567	1.39E-284	YJL166W	QCR8	hom	Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.543	9.72E-256	YDR230W_d	YDR230W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.505	1.90E-216	YPL215W	CBP3	hom	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.502	3.69E-213	YJR120W	YJR120W	hom	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
0.501	1.44E-212	YHR050W-A_p	YHR050W-A_p	hom	Protein of unknown function; identified by expression profiling and mass spectrometry
0.499	3.50E-210	YHR116W	COX23	hom	Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.495	1.96E-206	YPL013C	MRPS16	hom	Mitochondrial ribosomal protein of the small subunit
0.493	1.82E-204	YMR035W	IMP2	hom	Catalytic subunit of the mitochondrial inner membrane peptidase complex, required for maturation of mitochondrial proteins of the intermembrane space; complex contains Imp1p and Imp2p (both catalytic subunits), and Som1p
0.489	1.39E-200	YOR330C	MIP1	hom	Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.488	5.86E-200	YDR375C	BCS1	hom	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.488	1.50E-199	YEL059C-A	SOM1	hom	Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.487	5.86E-199	YMR151W_d	YIM2_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.471	1.94E-184	YOR065W	CYT1	hom	Cytochrome c1, component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
0.467	2.85E-181	YPL132W	COX11	hom	Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.467	4.61E-181	YGL237C	HAP2	hom	Subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding