YKL210W / UBA1

Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKL210W / UBA1

Click on Significant Values for Screen Details ID:SGTC_29|Compound:0299-0004|FD-Score:-3.40|P-value:2.75E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.24|P-value:8.86E-6 ID:SGTC_90|Compound:0870-0863|FD-Score:3.57|P-value:1.50E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-4.81|P-value:5.05E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:5.33|P-value:3.40E-8 ID:SGTC_266|Compound:formosanin C|FD-Score:3.34|P-value:3.66E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.29|P-value:4.30E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.07|P-value:9.00E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.85|P-value:4.86E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.40|P-value:2.94E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.19|P-value:5.82E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:9.03|P-value:2.76E-20 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.70|P-value:8.90E-5 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.40|P-value:2.86E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.72|P-value:8.89E-7 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.72|P-value:7.78E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.30|P-value:4.12E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.23|P-value:5.34E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.25|P-value:5.05E-4 ID:SGTC_1761|Compound:st044296|FD-Score:3.45|P-value:2.42E-4 ID:SGTC_1884|Compound:5633444|FD-Score:6.25|P-value:1.24E-10 ID:SGTC_2075|Compound:5212009|FD-Score:3.18|P-value:6.54E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.14|P-value:7.07E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.13|P-value:7.59E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.38|P-value:4.69E-6 ID:SGTC_2264|Compound:7948757|FD-Score:4.77|P-value:6.92E-7 ID:SGTC_2271|Compound:7849410|FD-Score:-3.97|P-value:2.68E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-3.15|P-value:6.64E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.22|P-value:5.68E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-3.15|P-value:6.71E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.35|P-value:3.46E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.73|P-value:7.86E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:4.05|P-value:2.06E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.80|P-value:5.70E-7 ID:SGTC_2812|Compound:7970281|FD-Score:3.84|P-value:5.14E-5 ID:SGTC_2873|Compound:9040596|FD-Score:3.20|P-value:6.09E-4 ID:SGTC_2902|Compound:5787006|FD-Score:4.71|P-value:8.99E-7 ID:SGTC_2908|Compound:7930301|FD-Score:-4.21|P-value:9.25E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.37|P-value:3.28E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.48|P-value:2.16E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.98|P-value:2.77E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.26|P-value:7.92E-6 ID:SGTC_3088|Compound:9117578|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.36|P-value:3.30E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.17|P-value:6.67E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.63|P-value:1.18E-4 ID:SGTC_3197|Compound:9113331|FD-Score:4.59|P-value:1.65E-6 ID:SGTC_3246|Compound:9135460|FD-Score:3.36|P-value:3.39E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.40|P-value:2.93E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.30|P-value:3.90E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-4.00|P-value:2.32E-5 ID:SGTC_3303|Compound:9125618|FD-Score:4.34|P-value:5.51E-6 ID:SGTC_29|Compound:0299-0004|FD-Score:-3.40|P-value:2.75E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.24|P-value:8.86E-6 ID:SGTC_90|Compound:0870-0863|FD-Score:3.57|P-value:1.50E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:-4.81|P-value:5.05E-7 ID:SGTC_168|Compound:3970-0862|FD-Score:5.33|P-value:3.40E-8 ID:SGTC_266|Compound:formosanin C|FD-Score:3.34|P-value:3.66E-4 ID:SGTC_333|Compound:3486-0131|FD-Score:3.29|P-value:4.30E-4 ID:SGTC_490|Compound:phenamil|FD-Score:-3.07|P-value:9.00E-4 ID:SGTC_533|Compound:1222-0222|FD-Score:3.85|P-value:4.86E-5 ID:SGTC_865|Compound:0488-0499|FD-Score:3.40|P-value:2.94E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:-3.19|P-value:5.82E-4 ID:SGTC_932|Compound:2857-0803|FD-Score:9.03|P-value:2.76E-20 ID:SGTC_1055|Compound:3448-0672|FD-Score:3.70|P-value:8.90E-5 ID:SGTC_1223|Compound:0764-0573|FD-Score:3.40|P-value:2.86E-4 ID:SGTC_1299|Compound:1079-1439|FD-Score:3.63|P-value:1.19E-4 ID:SGTC_1304|Compound:1165-0502|FD-Score:4.72|P-value:8.89E-7 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.72|P-value:7.78E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:3.30|P-value:4.12E-4 ID:SGTC_1735|Compound:st034310|FD-Score:3.23|P-value:5.34E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.25|P-value:5.05E-4 ID:SGTC_1761|Compound:st044296|FD-Score:3.45|P-value:2.42E-4 ID:SGTC_1884|Compound:5633444|FD-Score:6.25|P-value:1.24E-10 ID:SGTC_2075|Compound:5212009|FD-Score:3.18|P-value:6.54E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.14|P-value:7.07E-4 ID:SGTC_2172|Compound:5754775|FD-Score:3.13|P-value:7.59E-4 ID:SGTC_2233|Compound:sulbentine|FD-Score:4.38|P-value:4.69E-6 ID:SGTC_2264|Compound:7948757|FD-Score:4.77|P-value:6.92E-7 ID:SGTC_2271|Compound:7849410|FD-Score:-3.97|P-value:2.68E-5 ID:SGTC_2272|Compound:7805186|FD-Score:-3.15|P-value:6.64E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.22|P-value:5.68E-4 ID:SGTC_2481|Compound:5478948|FD-Score:-3.15|P-value:6.71E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.35|P-value:3.46E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:3.73|P-value:7.86E-5 ID:SGTC_2725|Compound:haloperidol|FD-Score:4.05|P-value:2.06E-5 ID:SGTC_2806|Compound:7952793|FD-Score:4.80|P-value:5.70E-7 ID:SGTC_2812|Compound:7970281|FD-Score:3.84|P-value:5.14E-5 ID:SGTC_2873|Compound:9040596|FD-Score:3.20|P-value:6.09E-4 ID:SGTC_2902|Compound:5787006|FD-Score:4.71|P-value:8.99E-7 ID:SGTC_2908|Compound:7930301|FD-Score:-4.21|P-value:9.25E-6 ID:SGTC_2910|Compound:7948405|FD-Score:3.37|P-value:3.28E-4 ID:SGTC_2959|Compound:9079155|FD-Score:3.48|P-value:2.16E-4 ID:SGTC_2963|Compound:9082507|FD-Score:3.98|P-value:2.77E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:4.26|P-value:7.92E-6 ID:SGTC_3088|Compound:9117578|FD-Score:3.57|P-value:1.53E-4 ID:SGTC_3138|Compound:9097216|FD-Score:3.36|P-value:3.30E-4 ID:SGTC_3159|Compound:9100096|FD-Score:3.17|P-value:6.67E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.63|P-value:1.18E-4 ID:SGTC_3197|Compound:9113331|FD-Score:4.59|P-value:1.65E-6 ID:SGTC_3246|Compound:9135460|FD-Score:3.36|P-value:3.39E-4 ID:SGTC_3294|Compound:9122953|FD-Score:3.40|P-value:2.93E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.30|P-value:3.90E-4 ID:SGTC_3302|Compound:9125395|FD-Score:-4.00|P-value:2.32E-5 ID:SGTC_3303|Compound:9125618|FD-Score:4.34|P-value:5.51E-6

Top fitness defect scores for YKL210W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_932 2857-0803 9.03 154.0 µM 2.76E-20 1 8.64E-20 2.30
2 SGTC_1884 5633444 6.25 25.0 µM 1.24E-10 redox potentiating 6 2.11E-10 0.70
3 SGTC_168 3970-0862 5.33 530.5 µM 3.40E-8 25 5.00E-8 0.09
4 SGTC_2806 7952793 4.80 58.4 µM 5.70E-7 2 7.78E-7 0.56
5 SGTC_2264 7948757 4.77 110.8 µM 6.92E-7 1 9.39E-7 0.69
6 SGTC_1304 1165-0502 4.72 251.0 µM 8.89E-7 2 1.20E-6 0.36
7 SGTC_2902 5787006 4.71 60.5 µM 8.99E-7 Golgi 2 1.21E-6 0.72
8 SGTC_3197 9113331 4.59 49.5 µM 1.65E-6 6 2.19E-6 0.20
9 SGTC_2233 sulbentine 4.38 40.4 µM 4.69E-6 32 6.06E-6 0.12
10 SGTC_3303 9125618 4.34 40.6 µM 5.51E-6 5 7.09E-6 0.13
11 SGTC_3059 1327-0034 4.26 3.0 µM 7.92E-6 10 1.01E-5 0.26
12 SGTC_77 0828-0286 4.24 12.5 µM 8.86E-6 TSC3-RPN4 5 1.13E-5 0.02
13 SGTC_2725 haloperidol 4.05 53.2 µM 2.06E-5 plasma membrane duress 16 2.56E-5 0.16
14 SGTC_2963 9082507 3.98 9.0 µM 2.77E-5 8 3.42E-5 0.23
15 SGTC_533 1222-0222 3.85 6.7 µM 4.86E-5 PDR1 14 5.92E-5 0.32
16 SGTC_2812 7970281 3.84 58.4 µM 5.14E-5 10 6.25E-5 0.04
17 SGTC_2607 solasodine 3.73 47.4 µM 7.86E-5 16 9.44E-5 0.00
18 SGTC_1055 3448-0672 3.70 86.0 µM 8.90E-5 16 1.07E-4 0.05
19 SGTC_3188 9110789 3.63 49.5 µM 1.18E-4 iron homeostasis 12 1.40E-4 0.13
20 SGTC_1299 1079-1439 3.63 3.7 µM 1.19E-4 TSC3-RPN4 6 1.42E-4 0.19

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 4.46E-11 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.113 5.47E-11 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.108 3.37E-10 YOR350C MNE1 hom Mitochondrial matrix protein involved in splicing Group I aI5-beta intron from COX1 mRNA
0.098 1.30E-8 YPR060C ARO7 hom Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
0.097 2.11E-8 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.095 3.57E-8 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.092 9.12E-8 YHR025W THR1 hom Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.092 1.04E-7 YHL011C PRS3 hom 5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
0.090 1.79E-7 YLR191W PEX13 hom Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p
0.087 4.71E-7 YMR126C_p DLT1_p hom Protein of unknown function, mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
0.084 1.01E-6 YLL038C ENT4 hom Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.083 1.73E-6 YDR176W NGG1 hom Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
0.082 1.89E-6 YDR432W NPL3 hom RNA-binding protein; promotes elongation, regulates termination, and carries poly(A) mRNA from nucleus to cytoplasm; has a role in repressing translation initiation by binding eIF4G; required for pre-mRNA splicing; dissociation from mRNAs promoted by Mtr10p; phosphorylated by Sky1p in the cytoplasm; protein abundance increases in response to DNA replication stress
0.080 3.24E-6 YCL046W_d YCL046W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
0.078 5.85E-6 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication