YKL213C / DOA1

WD repeat protein required for ubiquitin-mediated protein degradation; forms a complex with Cdc48p; plays a role in controlling cellular ubiquitin concentration; also promotes efficient NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL213C / DOA1

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.99|P-value:3.31E-5 ID:SGTC_51|Compound:1013-0126|FD-Score:3.49|P-value:2.31E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.45|P-value:6.69E-14 ID:SGTC_103|Compound:0844-0104|FD-Score:8.93|P-value:4.00E-19 ID:SGTC_173|Compound:1273-0061|FD-Score:3.63|P-value:1.38E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:-3.73|P-value:1.48E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.99|P-value:5.41E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:4.69|P-value:1.45E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.06|P-value:4.11E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:4.90|P-value:5.20E-7 ID:SGTC_513|Compound:4245-1575|FD-Score:10.90|P-value:2.26E-27 ID:SGTC_568|Compound:1193-0097|FD-Score:4.35|P-value:6.92E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:3.35|P-value:3.86E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:17.70|P-value:6.03E-69 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.52|P-value:3.27E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:3.12|P-value:8.66E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:5.55E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:-3.42|P-value:4.67E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.00|P-value:3.21E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.23|P-value:5.94E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:-3.30|P-value:7.04E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.27|P-value:5.21E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.55|P-value:1.87E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-4.46|P-value:7.43E-6 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.09|P-value:9.39E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-3.87|P-value:8.58E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:3.34|P-value:4.03E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.97|P-value:5.95E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.45|P-value:4.39E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.89|P-value:4.91E-5 ID:SGTC_2007|Compound:4011539|FD-Score:4.69|P-value:1.40E-6 ID:SGTC_2030|Compound:5175110|FD-Score:5.85|P-value:2.95E-9 ID:SGTC_2053|Compound:5265679|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.52|P-value:3.12E-6 ID:SGTC_2099|Compound:5377090|FD-Score:3.25|P-value:5.51E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.69|P-value:1.09E-4 ID:SGTC_2147|Compound:5545932|FD-Score:-3.92|P-value:7.12E-5 ID:SGTC_2148|Compound:5546263|FD-Score:-4.45|P-value:7.78E-6 ID:SGTC_2150|Compound:5556613|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2167|Compound:5876490|FD-Score:4.62|P-value:1.95E-6 ID:SGTC_2292|Compound:7671323|FD-Score:3.37|P-value:3.57E-4 ID:SGTC_2295|Compound:7971645|FD-Score:4.75|P-value:1.06E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.18|P-value:7.05E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.50|P-value:4.94E-11 ID:SGTC_2469|Compound:5655019|FD-Score:4.05|P-value:2.59E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.24|P-value:5.76E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.31|P-value:4.52E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.10|P-value:1.55E-33 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.56|P-value:2.69E-6 ID:SGTC_2601|Compound:securinine|FD-Score:8.70|P-value:2.96E-18 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.17|P-value:1.53E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.97|P-value:3.56E-5 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.70|P-value:1.06E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.52|P-value:2.07E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.26|P-value:5.35E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.26|P-value:5.28E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.36|P-value:5.76E-4 ID:SGTC_2982|Compound:9019404|FD-Score:-3.59|P-value:2.52E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.32|P-value:6.62E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.30|P-value:4.67E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.46|P-value:2.61E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.99|P-value:3.31E-5 ID:SGTC_51|Compound:1013-0126|FD-Score:3.49|P-value:2.31E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:7.45|P-value:6.69E-14 ID:SGTC_103|Compound:0844-0104|FD-Score:8.93|P-value:4.00E-19 ID:SGTC_173|Compound:1273-0061|FD-Score:3.63|P-value:1.38E-4 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:-3.73|P-value:1.48E-4 ID:SGTC_407|Compound:eburnamonine|FD-Score:-3.99|P-value:5.41E-5 ID:SGTC_424|Compound:splitomicin|FD-Score:4.69|P-value:1.45E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:-4.06|P-value:4.11E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:4.90|P-value:5.20E-7 ID:SGTC_513|Compound:4245-1575|FD-Score:10.90|P-value:2.26E-27 ID:SGTC_568|Compound:1193-0097|FD-Score:4.35|P-value:6.92E-6 ID:SGTC_679|Compound:0422-0091|FD-Score:3.35|P-value:3.86E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:17.70|P-value:6.03E-69 ID:SGTC_892|Compound:0929-0076|FD-Score:-3.52|P-value:3.27E-4 ID:SGTC_898|Compound:1000-0849|FD-Score:3.12|P-value:8.66E-4 ID:SGTC_1035|Compound:k081-0041|FD-Score:3.25|P-value:5.55E-4 ID:SGTC_1158|Compound:4092-0494|FD-Score:-3.42|P-value:4.67E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.00|P-value:3.21E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.23|P-value:5.94E-4 ID:SGTC_1277|Compound:0873-0036|FD-Score:-3.30|P-value:7.04E-4 ID:SGTC_1382|Compound:3002-2117|FD-Score:3.27|P-value:5.21E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.55|P-value:1.87E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:-4.46|P-value:7.43E-6 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.09|P-value:9.39E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-3.87|P-value:8.58E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:3.34|P-value:4.03E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.97|P-value:5.95E-5 ID:SGTC_1750|Compound:st081859|FD-Score:4.45|P-value:4.39E-6 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.89|P-value:4.91E-5 ID:SGTC_2007|Compound:4011539|FD-Score:4.69|P-value:1.40E-6 ID:SGTC_2030|Compound:5175110|FD-Score:5.85|P-value:2.95E-9 ID:SGTC_2053|Compound:5265679|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.52|P-value:3.12E-6 ID:SGTC_2099|Compound:5377090|FD-Score:3.25|P-value:5.51E-4 ID:SGTC_2109|Compound:5483026|FD-Score:3.69|P-value:1.09E-4 ID:SGTC_2147|Compound:5545932|FD-Score:-3.92|P-value:7.12E-5 ID:SGTC_2148|Compound:5546263|FD-Score:-4.45|P-value:7.78E-6 ID:SGTC_2150|Compound:5556613|FD-Score:-3.76|P-value:1.35E-4 ID:SGTC_2167|Compound:5876490|FD-Score:4.62|P-value:1.95E-6 ID:SGTC_2292|Compound:7671323|FD-Score:3.37|P-value:3.57E-4 ID:SGTC_2295|Compound:7971645|FD-Score:4.75|P-value:1.06E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.18|P-value:7.05E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_2399|Compound:5884133|FD-Score:6.50|P-value:4.94E-11 ID:SGTC_2469|Compound:5655019|FD-Score:4.05|P-value:2.59E-5 ID:SGTC_2532|Compound:xanthone|FD-Score:3.24|P-value:5.76E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.31|P-value:4.52E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:12.10|P-value:1.55E-33 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.56|P-value:2.69E-6 ID:SGTC_2601|Compound:securinine|FD-Score:8.70|P-value:2.96E-18 ID:SGTC_2679|Compound:rapamycin|FD-Score:4.17|P-value:1.53E-5 ID:SGTC_2690|Compound:usnic acid|FD-Score:3.97|P-value:3.56E-5 ID:SGTC_2718|Compound:lomofungin|FD-Score:3.70|P-value:1.06E-4 ID:SGTC_2799|Compound:7812892|FD-Score:3.52|P-value:2.07E-4 ID:SGTC_2814|Compound:7977482|FD-Score:3.26|P-value:5.35E-4 ID:SGTC_2846|Compound:9008642|FD-Score:3.26|P-value:5.28E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-3.36|P-value:5.76E-4 ID:SGTC_2982|Compound:9019404|FD-Score:-3.59|P-value:2.52E-4 ID:SGTC_3081|Compound:9117243|FD-Score:-3.32|P-value:6.62E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.30|P-value:4.67E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.46|P-value:2.61E-4

Top fitness defect scores for YKL213C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_690 glyoxal 17.70 20.3 µM 6.03E-69 2 1.71E-70
2 SGTC_2582 agaric acid 12.10 100.0 µM 1.55E-33 redox potentiating 39 3.65E-34
3 SGTC_513 4245-1575 10.90 41.7 µM 2.26E-27 unfolded protein response 12 7.53E-28
4 SGTC_103 0844-0104 8.93 51.8 µM 4.00E-19 ERAD & cell cycle 38 2.09E-19
5 SGTC_2601 securinine 8.70 100.0 µM 2.96E-18 1 1.62E-18
6 SGTC_58 0957-0393 7.45 9.0 µM 6.69E-14 cell wall 146 4.58E-14
7 SGTC_2399 5884133 6.50 200.0 µM 4.94E-11 cell wall signaling 25 3.89E-11
8 SGTC_2030 5175110 5.85 200.0 µM 2.95E-9 Golgi 5 2.52E-9
9 SGTC_431 k089-0097 4.90 107.0 µM 5.20E-7 iron homeostasis 15 4.89E-7
10 SGTC_2295 7971645 4.75 200.0 µM 1.06E-6 12 1.01E-6
11 SGTC_2007 4011539 4.69 71.8 µM 1.40E-6 cell wall signaling 39 1.34E-6
12 SGTC_424 splitomicin 4.69 100.0 mM 1.45E-6 9 1.38E-6
13 SGTC_2167 5876490 4.62 200.0 µM 1.95E-6 14 1.88E-6
14 SGTC_2599 2',3-dihydroxy-4,4',6'-trimethoxychalcone 4.56 100.0 µM 2.69E-6 21 2.60E-6
15 SGTC_2060 5238662 4.52 140.0 µM 3.12E-6 18 3.02E-6
16 SGTC_1750 st081859 4.45 2.4 µM 4.39E-6 endomembrane recycling 155 4.27E-6
17 SGTC_568 1193-0097 4.35 7.5 µM 6.92E-6 ERAD & cell cycle 124 6.79E-6
18 SGTC_2679 rapamycin 4.17 1.0 nM 1.53E-5 129 1.52E-5
19 SGTC_2469 5655019 4.05 90.8 µM 2.59E-5 21 2.59E-5
20 SGTC_1161 k007-0610 4.00 110.0 µM 3.21E-5 ERAD & cell cycle 119 3.23E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.295 2.31E-68 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.281 1.60E-61 YLR027C AAT2 hom Cytosolic aspartate aminotransferase, involved in nitrogen metabolism; localizes to peroxisomes in oleate-grown cells
0.270 9.84E-57 YBL024W NCL1 hom S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase, methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
0.258 4.31E-52 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.255 8.62E-51 YLR113W HOG1 hom Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNA Pol II in response to osmotic shock; acts via three independent osmosensors; mitophagy-specific regulator; mediates the recruitment and activation of RNA Pol II at Hot1p-dependent promoters; localization regulated by Ptp2p and Ptp3p; protein abundance increases in response to DNA replication stress
0.247 1.41E-47 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.230 2.99E-41 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.225 1.23E-39 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.222 1.95E-38 YPL179W PPQ1 hom Putative protein serine/threonine phosphatase; null mutation enhances efficiency of translational suppressors
0.221 2.74E-38 YML016C PPZ1 hom Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
0.215 2.05E-36 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.213 1.34E-35 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.212 4.02E-35 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.211 4.73E-35 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.210 1.54E-34 YOR364W_d YOR364W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C