YKL214C / YRA2

Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL214C / YRA2

Click on Significant Values for Screen Details ID:SGTC_107|Compound:0358-0022|FD-Score:-2.86|P-value:7.16E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:2.80|P-value:9.17E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.19|P-value:1.98E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.11|P-value:2.79E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.29|P-value:1.30E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-2.93|P-value:5.52E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.79|P-value:9.52E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.56|P-value:3.76E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:2.91|P-value:6.08E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.06|P-value:3.41E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:2.80|P-value:9.19E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.25|P-value:1.52E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.00|P-value:4.34E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.25|P-value:1.53E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.30|P-value:1.20E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.47|P-value:3.42E-7 ID:SGTC_778|Compound:3456-2434|FD-Score:3.28|P-value:1.34E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.71|P-value:1.88E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.50|P-value:5.03E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.83|P-value:8.19E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.30|P-value:1.25E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.03|P-value:3.60E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.16|P-value:2.17E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.28|P-value:1.33E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.24|P-value:1.61E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-2.92|P-value:5.77E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.81|P-value:8.91E-4 ID:SGTC_1830|Compound:st055982|FD-Score:4.73|P-value:7.33E-8 ID:SGTC_2160|Compound:5660466|FD-Score:3.14|P-value:2.41E-4 ID:SGTC_2211|Compound:7117706|FD-Score:2.97|P-value:4.91E-4 ID:SGTC_2255|Compound:7941087|FD-Score:5.68|P-value:1.39E-10 ID:SGTC_2307|Compound:7538926|FD-Score:-3.05|P-value:3.43E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-3.65|P-value:2.40E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.65|P-value:1.16E-7 ID:SGTC_2332|Compound:7982454|FD-Score:-2.86|P-value:7.08E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.56|P-value:3.78E-5 ID:SGTC_2396|Compound:5342950|FD-Score:3.01|P-value:4.21E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.21|P-value:1.43E-6 ID:SGTC_2439|Compound:5492030|FD-Score:3.67|P-value:2.27E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.09|P-value:3.01E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-2.79|P-value:9.52E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.48|P-value:5.56E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.50|P-value:2.88E-7 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.00|P-value:4.36E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.76|P-value:1.46E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.81|P-value:1.17E-5 ID:SGTC_2815|Compound:7968870|FD-Score:7.98|P-value:3.64E-19 ID:SGTC_2974|Compound:9094440|FD-Score:-3.02|P-value:3.78E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.55|P-value:3.93E-5 ID:SGTC_3000|Compound:9075062|FD-Score:-3.24|P-value:1.51E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-4.29|P-value:8.64E-7 ID:SGTC_3101|Compound:9120531|FD-Score:-3.48|P-value:5.40E-5 ID:SGTC_3230|Compound:9131665|FD-Score:2.99|P-value:4.46E-4 ID:SGTC_107|Compound:0358-0022|FD-Score:-2.86|P-value:7.16E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:2.80|P-value:9.17E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.19|P-value:1.98E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:3.11|P-value:2.79E-4 ID:SGTC_222|Compound:nsc-310342|FD-Score:3.29|P-value:1.30E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-2.93|P-value:5.52E-4 ID:SGTC_380|Compound:3970-0968|FD-Score:-2.79|P-value:9.52E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:3.56|P-value:3.76E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:2.91|P-value:6.08E-4 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.06|P-value:3.41E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:2.80|P-value:9.19E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:3.25|P-value:1.52E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.00|P-value:4.34E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.25|P-value:1.53E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.30|P-value:1.20E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:4.47|P-value:3.42E-7 ID:SGTC_778|Compound:3456-2434|FD-Score:3.28|P-value:1.34E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:3.71|P-value:1.88E-5 ID:SGTC_918|Compound:glabranin|FD-Score:3.50|P-value:5.03E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:2.83|P-value:8.19E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:3.30|P-value:1.25E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:-4.03|P-value:3.60E-6 ID:SGTC_1319|Compound:1306-0155|FD-Score:-3.16|P-value:2.17E-4 ID:SGTC_1588|Compound:farnesol|FD-Score:3.28|P-value:1.33E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.24|P-value:1.61E-4 ID:SGTC_1721|Compound:st082152|FD-Score:-2.92|P-value:5.77E-4 ID:SGTC_1779|Compound:st039427|FD-Score:2.81|P-value:8.91E-4 ID:SGTC_1830|Compound:st055982|FD-Score:4.73|P-value:7.33E-8 ID:SGTC_2160|Compound:5660466|FD-Score:3.14|P-value:2.41E-4 ID:SGTC_2211|Compound:7117706|FD-Score:2.97|P-value:4.91E-4 ID:SGTC_2255|Compound:7941087|FD-Score:5.68|P-value:1.39E-10 ID:SGTC_2307|Compound:7538926|FD-Score:-3.05|P-value:3.43E-4 ID:SGTC_2314|Compound:7727897|FD-Score:-3.65|P-value:2.40E-5 ID:SGTC_2331|Compound:7980721|FD-Score:4.65|P-value:1.16E-7 ID:SGTC_2332|Compound:7982454|FD-Score:-2.86|P-value:7.08E-4 ID:SGTC_2346|Compound:9031566|FD-Score:3.56|P-value:3.78E-5 ID:SGTC_2396|Compound:5342950|FD-Score:3.01|P-value:4.21E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.21|P-value:1.43E-6 ID:SGTC_2439|Compound:5492030|FD-Score:3.67|P-value:2.27E-5 ID:SGTC_2445|Compound:5742597|FD-Score:3.09|P-value:3.01E-4 ID:SGTC_2556|Compound:citropten|FD-Score:-2.79|P-value:9.52E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.48|P-value:5.56E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.50|P-value:2.88E-7 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.00|P-value:4.36E-4 ID:SGTC_2741|Compound:miltefosine|FD-Score:3.76|P-value:1.46E-5 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.81|P-value:1.17E-5 ID:SGTC_2815|Compound:7968870|FD-Score:7.98|P-value:3.64E-19 ID:SGTC_2974|Compound:9094440|FD-Score:-3.02|P-value:3.78E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.55|P-value:3.93E-5 ID:SGTC_3000|Compound:9075062|FD-Score:-3.24|P-value:1.51E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-4.29|P-value:8.64E-7 ID:SGTC_3101|Compound:9120531|FD-Score:-3.48|P-value:5.40E-5 ID:SGTC_3230|Compound:9131665|FD-Score:2.99|P-value:4.46E-4

Top fitness defect scores for YKL214C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2815 7968870 7.98 71.4 µM 3.64E-19 2 7.46E-16
2 SGTC_2255 7941087 5.68 197.4 µM 1.39E-10 12 6.89E-9
3 SGTC_1830 st055982 4.73 50.3 µM 7.33E-8 7 1.13E-6
4 SGTC_2331 7980721 4.65 200.0 µM 1.16E-7 9 1.64E-6
5 SGTC_2640 pentamidine 4.50 10.4 µM 2.88E-7 mitochondrial processes 20 3.44E-6
6 SGTC_773 4358-1457 4.47 44.6 µM 3.42E-7 31 3.95E-6
7 SGTC_2407 paclitaxel 4.21 400.0 µM 1.43E-6 26 1.27E-5
8 SGTC_2765 myclobutanil 3.81 7.7 µM 1.17E-5 azole & statin 53 7.04E-5
9 SGTC_2741 miltefosine 3.76 3.0 µM 1.46E-5 45 8.43E-5
10 SGTC_916 4466-0038 3.71 42.0 nM 1.88E-5 cell wall 81 1.04E-4
11 SGTC_2439 5492030 3.67 7.2 µM 2.27E-5 17 1.21E-4
12 SGTC_431 k089-0097 3.56 107.0 µM 3.76E-5 iron homeostasis 62 1.83E-4
13 SGTC_2346 9031566 3.56 143.8 µM 3.78E-5 Golgi 38 1.84E-4
14 SGTC_2991 9074669 3.55 71.4 µM 3.93E-5 azole & statin 81 1.90E-4
15 SGTC_918 glabranin 3.50 770.7 nM 5.03E-5 49 2.33E-4
16 SGTC_2612 dictamnine 3.48 15.5 µM 5.56E-5 RNA pol III & RNase P/MRP 62 2.52E-4
17 SGTC_1270 0831-1170 3.30 17.3 µM 1.25E-4 27 4.90E-4
18 SGTC_222 nsc-310342 3.29 10.7 µM 1.30E-4 9 5.06E-4
19 SGTC_1588 farnesol 3.28 29.9 µM 1.33E-4 27 5.16E-4
20 SGTC_778 3456-2434 3.28 196.0 µM 1.34E-4 mitochondrial response to ROS 112 5.18E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.177 7.06E-25 YLR212C TUB4 het Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress
0.150 2.32E-18 YHR190W ERG9 het Farnesyl-diphosphate farnesyl transferase (squalene synthase), joins two farnesyl pyrophosphate moieties to form squalene in the sterol biosynthesis pathway
0.126 2.59E-13 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.125 3.07E-13 YLR305C STT4 het Phosphatidylinositol-4-kinase that functions in the Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization
0.112 7.88E-11 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.110 1.79E-10 YKL066W_d YKL066W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
0.108 3.30E-10 YAL043C PTA1 het Subunit of holo-CPF, a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
0.108 3.84E-10 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.107 5.75E-10 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.104 1.37E-9 YDR053W_d YDR053W_d het Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
0.104 1.54E-9 YML100W TSL1 hom Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
0.104 1.55E-9 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.102 2.80E-9 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.100 6.54E-9 YPR123C_d YPR123C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
0.097 1.95E-8 YNL304W YPT11 hom Rab family GTPase that interacts with the C-terminal tail domain of Myo2p; mediates distribution of mitochondria and endoplasmic reticuli to daughter cells