YKL216W / URA1

Dihydroorotate dehydrogenase, catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL216W / URA1

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:-3.23|P-value:5.69E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.40|P-value:2.38E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.44|P-value:2.04E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.23|P-value:4.63E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-4.28|P-value:7.64E-6 ID:SGTC_171|Compound:k064-0041|FD-Score:4.98|P-value:1.75E-7 ID:SGTC_185|Compound:k048-0037|FD-Score:4.04|P-value:1.74E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:3.08|P-value:7.83E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:4.51|P-value:1.90E-6 ID:SGTC_671|Compound:0560-0003|FD-Score:3.50|P-value:1.63E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.82|P-value:4.06E-7 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.11|P-value:8.56E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:-4.98|P-value:2.29E-7 ID:SGTC_912|Compound:3474-0017|FD-Score:4.27|P-value:6.19E-6 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.14|P-value:7.81E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.17|P-value:5.67E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.25|P-value:5.19E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.14|P-value:6.37E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.77|P-value:5.49E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.94|P-value:2.10E-7 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.02|P-value:9.73E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:5.40|P-value:1.63E-8 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.27|P-value:3.88E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.76|P-value:5.79E-5 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.64|P-value:9.36E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.22|P-value:5.82E-4 ID:SGTC_1617|Compound:st002906|FD-Score:4.72|P-value:6.71E-7 ID:SGTC_1672|Compound:st016598|FD-Score:5.46|P-value:1.20E-8 ID:SGTC_1676|Compound:st018489|FD-Score:3.40|P-value:2.44E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.73|P-value:6.46E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-3.97|P-value:2.96E-5 ID:SGTC_1796|Compound:st049173|FD-Score:3.09|P-value:7.55E-4 ID:SGTC_2104|Compound:5389443|FD-Score:4.24|P-value:7.05E-6 ID:SGTC_2248|Compound:tofa|FD-Score:13.80|P-value:2.64E-45 ID:SGTC_2291|Compound:7917025|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_2311|Compound:7694451|FD-Score:-3.99|P-value:2.75E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.63|P-value:9.80E-5 ID:SGTC_2389|Compound:9091542|FD-Score:3.04|P-value:8.99E-4 ID:SGTC_2416|Compound:tofa|FD-Score:9.41|P-value:3.63E-22 ID:SGTC_2473|Compound:5854593|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.12|P-value:8.16E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.42|P-value:3.00E-6 ID:SGTC_2643|Compound:anethole|FD-Score:3.04|P-value:8.84E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.97|P-value:2.33E-5 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.90|P-value:4.09E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-3.08|P-value:9.41E-4 ID:SGTC_2813|Compound:7971799|FD-Score:4.12|P-value:1.19E-5 ID:SGTC_2814|Compound:7977482|FD-Score:5.53|P-value:7.70E-9 ID:SGTC_2833|Compound:9001315|FD-Score:4.39|P-value:3.47E-6 ID:SGTC_2844|Compound:9010103|FD-Score:3.16|P-value:5.92E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-5.47|P-value:1.53E-8 ID:SGTC_2942|Compound:9052777|FD-Score:3.74|P-value:6.40E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.69|P-value:7.61E-5 ID:SGTC_2954|Compound:9074818|FD-Score:-5.00|P-value:2.15E-7 ID:SGTC_2979|Compound:9012552|FD-Score:3.25|P-value:4.29E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.39|P-value:2.53E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.66|P-value:8.69E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-4.36|P-value:5.27E-6 ID:SGTC_3278|Compound:9139605|FD-Score:-3.76|P-value:7.21E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.54|P-value:1.42E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.67|P-value:1.04E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-3.36|P-value:3.42E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:-3.23|P-value:5.69E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.40|P-value:2.38E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.44|P-value:2.04E-4 ID:SGTC_153|Compound:4077-0274|FD-Score:3.23|P-value:4.63E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:-4.28|P-value:7.64E-6 ID:SGTC_171|Compound:k064-0041|FD-Score:4.98|P-value:1.75E-7 ID:SGTC_185|Compound:k048-0037|FD-Score:4.04|P-value:1.74E-5 ID:SGTC_187|Compound:1488-2104|FD-Score:3.08|P-value:7.83E-4 ID:SGTC_189|Compound:3970-0742|FD-Score:4.51|P-value:1.90E-6 ID:SGTC_671|Compound:0560-0003|FD-Score:3.50|P-value:1.63E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:4.82|P-value:4.06E-7 ID:SGTC_901|Compound:1112-0062|FD-Score:-3.11|P-value:8.56E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:-4.98|P-value:2.29E-7 ID:SGTC_912|Compound:3474-0017|FD-Score:4.27|P-value:6.19E-6 ID:SGTC_944|Compound:3370-0224|FD-Score:-3.14|P-value:7.81E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.34|P-value:3.00E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.17|P-value:5.67E-4 ID:SGTC_1120|Compound:ficusin|FD-Score:-3.25|P-value:5.19E-4 ID:SGTC_1153|Compound:4055-0057|FD-Score:3.14|P-value:6.37E-4 ID:SGTC_1232|Compound:0279-0259|FD-Score:3.77|P-value:5.49E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.94|P-value:2.10E-7 ID:SGTC_1300|Compound:1094-0077|FD-Score:3.02|P-value:9.73E-4 ID:SGTC_1375|Compound:2910-0797|FD-Score:5.40|P-value:1.63E-8 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.27|P-value:3.88E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.76|P-value:5.79E-5 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.64|P-value:9.36E-5 ID:SGTC_1564|Compound:D-Arginine|FD-Score:-3.22|P-value:5.82E-4 ID:SGTC_1617|Compound:st002906|FD-Score:4.72|P-value:6.71E-7 ID:SGTC_1672|Compound:st016598|FD-Score:5.46|P-value:1.20E-8 ID:SGTC_1676|Compound:st018489|FD-Score:3.40|P-value:2.44E-4 ID:SGTC_1689|Compound:st020265|FD-Score:3.73|P-value:6.46E-5 ID:SGTC_1727|Compound:st034309|FD-Score:-3.97|P-value:2.96E-5 ID:SGTC_1796|Compound:st049173|FD-Score:3.09|P-value:7.55E-4 ID:SGTC_2104|Compound:5389443|FD-Score:4.24|P-value:7.05E-6 ID:SGTC_2248|Compound:tofa|FD-Score:13.80|P-value:2.64E-45 ID:SGTC_2291|Compound:7917025|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_2311|Compound:7694451|FD-Score:-3.99|P-value:2.75E-5 ID:SGTC_2358|Compound:9037157|FD-Score:3.63|P-value:9.80E-5 ID:SGTC_2389|Compound:9091542|FD-Score:3.04|P-value:8.99E-4 ID:SGTC_2416|Compound:tofa|FD-Score:9.41|P-value:3.63E-22 ID:SGTC_2473|Compound:5854593|FD-Score:3.56|P-value:1.32E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:-3.12|P-value:8.16E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.42|P-value:3.00E-6 ID:SGTC_2643|Compound:anethole|FD-Score:3.04|P-value:8.84E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.97|P-value:2.33E-5 ID:SGTC_2751|Compound:anetholtrithion|FD-Score:-3.90|P-value:4.09E-5 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:3.45|P-value:2.00E-4 ID:SGTC_2810|Compound:7959871|FD-Score:-3.08|P-value:9.41E-4 ID:SGTC_2813|Compound:7971799|FD-Score:4.12|P-value:1.19E-5 ID:SGTC_2814|Compound:7977482|FD-Score:5.53|P-value:7.70E-9 ID:SGTC_2833|Compound:9001315|FD-Score:4.39|P-value:3.47E-6 ID:SGTC_2844|Compound:9010103|FD-Score:3.16|P-value:5.92E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-5.47|P-value:1.53E-8 ID:SGTC_2942|Compound:9052777|FD-Score:3.74|P-value:6.40E-5 ID:SGTC_2953|Compound:9074162|FD-Score:3.69|P-value:7.61E-5 ID:SGTC_2954|Compound:9074818|FD-Score:-5.00|P-value:2.15E-7 ID:SGTC_2979|Compound:9012552|FD-Score:3.25|P-value:4.29E-4 ID:SGTC_3118|Compound:9124250|FD-Score:3.39|P-value:2.53E-4 ID:SGTC_3174|Compound:9107204|FD-Score:3.66|P-value:8.69E-5 ID:SGTC_3264|Compound:9137442|FD-Score:-4.36|P-value:5.27E-6 ID:SGTC_3278|Compound:9139605|FD-Score:-3.76|P-value:7.21E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.54|P-value:1.42E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.67|P-value:1.04E-4 ID:SGTC_3355|Compound:9151058|FD-Score:-3.36|P-value:3.42E-4

Top fitness defect scores for YKL216W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2248 tofa 13.80 1.1 µM 2.64E-45 sphingolipid biosynthesis & PDR1 5 1.22E-43
2 SGTC_2416 tofa 9.41 880.0 nM 3.63E-22 sphingolipid biosynthesis & PDR1 12 2.39E-21
3 SGTC_2814 7977482 5.53 43.0 µM 7.70E-9 11 1.60E-8
4 SGTC_1672 st016598 5.46 6.5 µM 1.20E-8 endomembrane recycling 36 2.45E-8
5 SGTC_1375 2910-0797 5.40 186.0 µM 1.63E-8 9 3.29E-8
6 SGTC_171 k064-0041 4.98 9.7 µM 1.75E-7 heme requiring 17 3.22E-7
7 SGTC_1276 0871-0068 4.94 94.2 µM 2.10E-7 7 3.84E-7
8 SGTC_899 1083-0020 4.82 478.0 µM 4.06E-7 8 7.24E-7
9 SGTC_1617 st002906 4.72 78.0 µM 6.71E-7 16 1.17E-6
10 SGTC_189 3970-0742 4.51 2.1 µM 1.90E-6 5 3.19E-6
11 SGTC_2617 dihydrotanshinone i 4.42 20.8 µM 3.00E-6 copper-dependent oxidative stress 22 4.94E-6
12 SGTC_2833 9001315 4.39 45.5 µM 3.47E-6 amide catabolism 40 5.69E-6
13 SGTC_912 3474-0017 4.27 2.2 µM 6.19E-6 16 9.93E-6
14 SGTC_2104 5389443 4.24 200.0 µM 7.05E-6 8 1.13E-5
15 SGTC_2813 7971799 4.12 71.4 µM 1.19E-5 19 1.85E-5
16 SGTC_185 k048-0037 4.04 25.5 µM 1.74E-5 RSC & ERG11 10 2.69E-5
17 SGTC_2749 bexarotene 3.97 57.4 µM 2.33E-5 23 3.55E-5
18 SGTC_1232 0279-0259 3.77 161.0 µM 5.49E-5 21 8.09E-5
19 SGTC_1531 st059297 3.76 52.4 µM 5.79E-5 20 8.52E-5
20 SGTC_2942 9052777 3.74 62.2 µM 6.40E-5 40 9.37E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.303 6.17E-72 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.217 3.59E-37 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.200 1.11E-31 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
0.199 2.22E-31 YGL112C TAF6 het Subunit (60 kDa) of TFIID and SAGA complexes, involved in transcription initiation of RNA polymerase II and in chromatin modification, similar to histone H4
0.187 7.92E-28 YER177W BMH1 hom 14-3-3 protein, major isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
0.170 2.93E-23 YJR059W PTK2 hom Putative serine/threonine protein kinase involved in regulation of ion transport across plasma membrane; enhances spermine uptake
0.149 4.17E-18 YDR531W CAB1 het Pantothenate kinase (ATP:D-pantothenate 4'-phosphotransferase, EC 2.7.1.33); catalyzes the first committed step in the universal biosynthetic pathway for synthesis of coenzyme A (CoA); transcriptionally regulated by Upc2p via a sterol response element
0.143 8.42E-17 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.132 1.77E-14 YLR327C TMA10 hom Protein of unknown function that associates with ribosomes; putative homolog of the F1F0-ATPase synthase regulator Stf2p; protein abundance increases in response to DNA replication stress
0.132 1.47E-14 YOL078W AVO1 het Component of a membrane-bound complex containing the Tor2p kinase and other proteins, which may have a role in regulation of cell growth
0.129 7.12E-14 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.109 2.34E-10 YDR011W SNQ2 hom Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
0.108 4.08E-10 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.102 3.70E-9 YDR208W MSS4 het Phosphatidylinositol-4-phosphate 5-kinase, involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
0.096 2.63E-8 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export