YKL218C / SRY1

3-hydroxyaspartate dehydratase, deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL218C / SRY1

Click on Significant Values for Screen Details ID:SGTC_32|Compound:0928-0142|FD-Score:-2.97|P-value:6.23E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-2.89|P-value:8.50E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:3.48|P-value:9.67E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.44|P-value:1.13E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:4.96|P-value:4.90E-8 ID:SGTC_480|Compound:am 92016|FD-Score:3.35|P-value:1.68E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.10|P-value:4.46E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.30|P-value:2.06E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.17|P-value:3.43E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:4.19|P-value:3.41E-6 ID:SGTC_864|Compound:0469-0573|FD-Score:3.33|P-value:1.77E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.33|P-value:1.77E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.01|P-value:6.24E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.05|P-value:7.01E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.18|P-value:3.26E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-2.96|P-value:6.57E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.46|P-value:1.02E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.32|P-value:1.56E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.80|P-value:1.29E-7 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.14|P-value:3.83E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.77|P-value:2.57E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.03|P-value:6.29E-6 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.40|P-value:1.32E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.56|P-value:6.80E-5 ID:SGTC_1629|Compound:st005085|FD-Score:3.43|P-value:1.17E-4 ID:SGTC_1637|Compound:st007924|FD-Score:3.72|P-value:3.35E-5 ID:SGTC_1638|Compound:st008370|FD-Score:4.50|P-value:6.63E-7 ID:SGTC_1700|Compound:st030840|FD-Score:3.35|P-value:1.63E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.93|P-value:5.91E-8 ID:SGTC_1721|Compound:st082152|FD-Score:3.18|P-value:3.29E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-5.08|P-value:1.90E-8 ID:SGTC_1835|Compound:st008666|FD-Score:4.21|P-value:3.17E-6 ID:SGTC_1859|Compound:st055998|FD-Score:-3.22|P-value:2.41E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-5.32|P-value:4.34E-9 ID:SGTC_2055|Compound:5235184|FD-Score:3.57|P-value:6.49E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.33|P-value:1.75E-4 ID:SGTC_2453|Compound:5731132|FD-Score:-4.18|P-value:2.92E-6 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.27|P-value:2.29E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-4.08|P-value:4.92E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.15|P-value:3.73E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.13|P-value:3.93E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-3.54|P-value:6.29E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.60|P-value:5.54E-5 ID:SGTC_2998|Compound:9066762|FD-Score:4.59|P-value:4.19E-7 ID:SGTC_3004|Compound:9076122|FD-Score:2.93|P-value:8.63E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.22|P-value:2.80E-4 ID:SGTC_3212|Compound:9128307|FD-Score:5.17|P-value:1.38E-8 ID:SGTC_3217|Compound:9129673|FD-Score:-4.26|P-value:1.96E-6 ID:SGTC_3222|Compound:9128301|FD-Score:3.00|P-value:6.63E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-2.97|P-value:6.23E-4 ID:SGTC_50|Compound:0929-0078|FD-Score:-2.89|P-value:8.50E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:3.48|P-value:9.67E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:3.44|P-value:1.13E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:4.96|P-value:4.90E-8 ID:SGTC_480|Compound:am 92016|FD-Score:3.35|P-value:1.68E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.10|P-value:4.46E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.30|P-value:2.06E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.17|P-value:3.43E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:4.19|P-value:3.41E-6 ID:SGTC_864|Compound:0469-0573|FD-Score:3.33|P-value:1.77E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.33|P-value:1.77E-4 ID:SGTC_1030|Compound:k029-0042|FD-Score:3.01|P-value:6.24E-4 ID:SGTC_1041|Compound:0206-0046|FD-Score:4.05|P-value:7.01E-6 ID:SGTC_1045|Compound:1348-1485|FD-Score:3.18|P-value:3.26E-4 ID:SGTC_1055|Compound:3448-0672|FD-Score:-2.96|P-value:6.57E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.46|P-value:1.02E-4 ID:SGTC_1229|Compound:0139-0193|FD-Score:-3.32|P-value:1.56E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.80|P-value:1.29E-7 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.14|P-value:3.83E-4 ID:SGTC_1434|Compound:4073-0095|FD-Score:3.77|P-value:2.57E-5 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.03|P-value:6.29E-6 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.40|P-value:1.32E-4 ID:SGTC_1625|Compound:st003711|FD-Score:3.56|P-value:6.80E-5 ID:SGTC_1629|Compound:st005085|FD-Score:3.43|P-value:1.17E-4 ID:SGTC_1637|Compound:st007924|FD-Score:3.72|P-value:3.35E-5 ID:SGTC_1638|Compound:st008370|FD-Score:4.50|P-value:6.63E-7 ID:SGTC_1700|Compound:st030840|FD-Score:3.35|P-value:1.63E-4 ID:SGTC_1719|Compound:st032227|FD-Score:4.93|P-value:5.91E-8 ID:SGTC_1721|Compound:st082152|FD-Score:3.18|P-value:3.29E-4 ID:SGTC_1749|Compound:st037932|FD-Score:-5.08|P-value:1.90E-8 ID:SGTC_1835|Compound:st008666|FD-Score:4.21|P-value:3.17E-6 ID:SGTC_1859|Compound:st055998|FD-Score:-3.22|P-value:2.41E-4 ID:SGTC_1860|Compound:st056228|FD-Score:-5.32|P-value:4.34E-9 ID:SGTC_2055|Compound:5235184|FD-Score:3.57|P-value:6.49E-5 ID:SGTC_2439|Compound:5492030|FD-Score:3.33|P-value:1.75E-4 ID:SGTC_2453|Compound:5731132|FD-Score:-4.18|P-value:2.92E-6 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.27|P-value:2.29E-4 ID:SGTC_2692|Compound:st077426|FD-Score:-4.08|P-value:4.92E-6 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:3.15|P-value:3.73E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.13|P-value:3.93E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-3.54|P-value:6.29E-5 ID:SGTC_2950|Compound:9072895|FD-Score:3.60|P-value:5.54E-5 ID:SGTC_2998|Compound:9066762|FD-Score:4.59|P-value:4.19E-7 ID:SGTC_3004|Compound:9076122|FD-Score:2.93|P-value:8.63E-4 ID:SGTC_3200|Compound:9114385|FD-Score:3.22|P-value:2.80E-4 ID:SGTC_3212|Compound:9128307|FD-Score:5.17|P-value:1.38E-8 ID:SGTC_3217|Compound:9129673|FD-Score:-4.26|P-value:1.96E-6 ID:SGTC_3222|Compound:9128301|FD-Score:3.00|P-value:6.63E-4

Top fitness defect scores for YKL218C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3212 9128307 5.17 49.5 µM 1.38E-8 24 1.16E-7
2 SGTC_350 k064-0020 4.96 19.8 µM 4.90E-8 heme requiring 14 3.49E-7
3 SGTC_1719 st032227 4.93 49.2 µM 5.91E-8 12 4.10E-7
4 SGTC_1242 0402-0103 4.80 60.1 µM 1.29E-7 15 8.07E-7
5 SGTC_2998 9066762 4.59 71.4 µM 4.19E-7 RPP1 & pyrimidine depletion 13 2.24E-6
6 SGTC_1638 st008370 4.50 60.6 µM 6.63E-7 ergosterol biosynthesis 21 3.34E-6
7 SGTC_1835 st008666 4.21 49.2 µM 3.17E-6 20 1.30E-5
8 SGTC_766 1391-0721 4.19 126.0 µM 3.41E-6 10 1.39E-5
9 SGTC_1041 0206-0046 4.05 107.0 µM 7.01E-6 35 2.59E-5
10 SGTC_1434 4073-0095 3.77 163.0 µM 2.57E-5 12 8.03E-5
11 SGTC_1637 st007924 3.72 17.4 µM 3.35E-5 30 1.01E-4
12 SGTC_2950 9072895 3.60 21.5 µM 5.54E-5 55 1.57E-4
13 SGTC_2055 5235184 3.57 200.0 µM 6.49E-5 ERG2 50 1.80E-4
14 SGTC_1625 st003711 3.56 30.1 µM 6.80E-5 calcium & mitochondrial duress 50 1.87E-4
15 SGTC_117 lucigenin 3.48 22.1 µM 9.67E-5 redox potentiating 110 2.55E-4
16 SGTC_1202 1988-0068 3.46 29.6 µM 1.02E-4 RNA processing & uracil transport 43 2.66E-4
17 SGTC_344 k064-0035 3.44 6.4 µM 1.13E-4 60S ribosome export 39 2.90E-4
18 SGTC_1629 st005085 3.43 44.9 µM 1.17E-4 RPP1 & pyrimidine depletion 55 3.01E-4
19 SGTC_1592 4',5'-dihydroxyflavone 3.40 8.8 µM 1.32E-4 60S ribosome export 29 3.33E-4
20 SGTC_1700 st030840 3.35 7.9 µM 1.63E-4 PDR1 38 4.02E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.128 8.98E-14 YHL003C LAG1 hom Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress
0.103 2.03E-9 YIL152W_p YIL152W_p hom Putative protein of unknown function
0.097 2.17E-8 YNL193W_p YNL193W_p hom Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
0.096 2.57E-8 YMR313C TGL3 hom Bifunctional enzyme with triacylglycerol lipase and lysophosphatidylethanolamine acyltransferase activity; responsible for all the triacylglycerol lipase activity of the lipid particle; required with Tgl4p for timely bud formation
0.093 6.94E-8 YPL167C REV3 hom Catalytic subunit of DNA polymerase zeta, involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair
0.091 1.30E-7 YLR179C YLR179C hom Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
0.090 1.70E-7 YMR173W-A_d YMR173W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
0.088 3.06E-7 YAR018C KIN3 hom Nonessential serine/threonine protein kinase; possible role in DNA damage response
0.087 4.55E-7 YJL116C NCA3 hom Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication
0.086 7.13E-7 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.085 9.79E-7 YPR009W SUT2 hom Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.083 1.65E-6 YDL093W PMT5 hom Protein O-mannosyltransferase, transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals
0.082 1.75E-6 YFR032C_p RRT5_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
0.082 1.76E-6 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.079 4.99E-6 YGL050W TYW3 hom tRNA methyltransferase required for synthesis of wybutosine, a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions