YKL221W / MCH2

Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport

Zygosity: Homozygous strain
fixedexpanded
Profile for YKL221W / MCH2

Click on Significant Values for Screen Details ID:SGTC_143|Compound:4032-1194|FD-Score:-3.81|P-value:1.16E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.27|P-value:2.17E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:4.36|P-value:1.55E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.48|P-value:4.39E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.67|P-value:2.30E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.20|P-value:9.89E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.38|P-value:6.12E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.22|P-value:9.34E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.13|P-value:3.93E-5 ID:SGTC_1121|Compound:fusaric acid|FD-Score:13.80|P-value:4.65E-40 ID:SGTC_1568|Compound:digitonin|FD-Score:4.09|P-value:4.64E-5 ID:SGTC_1608|Compound:st001473|FD-Score:3.71|P-value:1.98E-4 ID:SGTC_1739|Compound:st037196|FD-Score:-3.30|P-value:7.19E-4 ID:SGTC_1793|Compound:st048256|FD-Score:-3.34|P-value:6.14E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.85|P-value:1.00E-4 ID:SGTC_2103|Compound:5378138|FD-Score:-3.52|P-value:3.38E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.48|P-value:4.38E-4 ID:SGTC_2267|Compound:7931562|FD-Score:3.80|P-value:1.39E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.49|P-value:4.30E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.83|P-value:1.24E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:-3.81|P-value:1.16E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:4.27|P-value:2.17E-5 ID:SGTC_253|Compound:dyclonine|FD-Score:4.36|P-value:1.55E-5 ID:SGTC_281|Compound:1672-3440|FD-Score:3.48|P-value:4.39E-4 ID:SGTC_479|Compound:n-linoleoylglycine|FD-Score:3.67|P-value:2.30E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:-3.20|P-value:9.89E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.38|P-value:6.12E-4 ID:SGTC_1007|Compound:4101-0031|FD-Score:-3.22|P-value:9.34E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.13|P-value:3.93E-5 ID:SGTC_1121|Compound:fusaric acid|FD-Score:13.80|P-value:4.65E-40 ID:SGTC_1568|Compound:digitonin|FD-Score:4.09|P-value:4.64E-5 ID:SGTC_1608|Compound:st001473|FD-Score:3.71|P-value:1.98E-4 ID:SGTC_1739|Compound:st037196|FD-Score:-3.30|P-value:7.19E-4 ID:SGTC_1793|Compound:st048256|FD-Score:-3.34|P-value:6.14E-4 ID:SGTC_2052|Compound:5265182|FD-Score:-3.85|P-value:1.00E-4 ID:SGTC_2103|Compound:5378138|FD-Score:-3.52|P-value:3.38E-4 ID:SGTC_2163|Compound:5671233|FD-Score:3.48|P-value:4.38E-4 ID:SGTC_2267|Compound:7931562|FD-Score:3.80|P-value:1.39E-4 ID:SGTC_2433|Compound:5607874|FD-Score:3.49|P-value:4.30E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.83|P-value:1.24E-4

Top fitness defect scores for YKL221W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1121 fusaric acid 13.80 630.5 nM 4.65E-40 5 2.53E-43
2 SGTC_253 dyclonine 4.36 31.2 µM 1.55E-5 amide catabolism 15 6.63E-6
3 SGTC_248 streptozotocin 4.27 5.2 mM 2.17E-5 DNA damage response 19 9.56E-6
4 SGTC_1082 camptothecin 4.13 424.9 nM 3.93E-5 DNA damage response 43 1.82E-5
5 SGTC_1568 digitonin 4.09 820.0 nM 4.64E-5 sphingolipid biosynthesis & PDR1 27 2.18E-5
6 SGTC_2502 avocadyne acetate 3.83 18.6 µM 1.24E-4 26 6.35E-5
7 SGTC_2267 7931562 3.80 200.0 µM 1.39E-4 26 7.16E-5
8 SGTC_1608 st001473 3.71 40.5 µM 1.98E-4 44 1.05E-4
9 SGTC_479 n-linoleoylglycine 3.67 10.0 µM 2.30E-4 14 1.23E-4
10 SGTC_2433 5607874 3.49 27.8 µM 4.30E-4 32 2.43E-4
11 SGTC_2163 5671233 3.48 125.0 µM 4.38E-4 37 2.48E-4
12 SGTC_281 1672-3440 3.48 14.9 µM 4.39E-4 Golgi 11 2.49E-4
13 SGTC_962 1254-0300 3.38 21.5 µM 6.12E-4 41 3.56E-4
14 SGTC_280 3448-3607 3.15 3.0 µM 0.00130 60S ribosome export 25 8.08E-4
15 SGTC_1723 st034307 3.13 2.5 µM 0.00141 mitochondrial processes 61 8.83E-4
16 SGTC_1258 0671-0043 3.10 57.1 µM 0.00155 67 9.77E-4
17 SGTC_814 3342-0569 3.09 96.0 µM 0.00158 67 9.96E-4
18 SGTC_2276 7947513 2.92 42.1 µM 0.00269 72 0.00177
19 SGTC_2343 7990716 2.90 14.8 µM 0.00283 61 0.00187
20 SGTC_98 0074-0023 2.88 107.7 µM 0.00297 Golgi 92 0.00197

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.361 5.28E-104 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.330 3.50E-86 YKL222C YKL222C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
0.324 9.27E-83 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.270 2.84E-57 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.167 2.03E-22 YJL172W CPS1 hom Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions
0.160 1.02E-20 YLR079W SIC1 hom Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
0.144 5.25E-17 YGL159W_p YGL159W_p hom Putative protein of unknown function; deletion mutant has no detectable phenotype
0.142 1.74E-16 YGR226C_d YGR226C_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
0.141 2.11E-16 YJL107C_p YJL107C_p hom Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
0.136 2.34E-15 YJL212C OPT1 hom Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
0.130 4.07E-14 YNL094W APP1 hom Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
0.127 1.30E-13 YMR087W YMR087W hom Putative ADP-ribose-1''-monophosphatase that converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
0.126 2.43E-13 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.120 2.75E-12 YGL096W TOS8 hom Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
0.119 4.12E-12 YDR363W ESC2 hom Sumo-like domain protein; prevents accumulation of toxic intermediates during replication-associated recombinational repair; roles in silencing, lifespan, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member