YKR001C / VPS1

Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR001C / VPS1

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:5.18|P-value:1.87E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.52|P-value:3.60E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.87|P-value:4.12E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.65|P-value:7.05E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.61|P-value:7.84E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:-4.50|P-value:8.46E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:4.98|P-value:3.13E-4 ID:SGTC_232|Compound:clozapine|FD-Score:4.92|P-value:3.67E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.74|P-value:3.93E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.86|P-value:4.18E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.83|P-value:3.74E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-4.52|P-value:7.96E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-4.66|P-value:5.80E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.84|P-value:2.33E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:5.22|P-value:1.68E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.58|P-value:6.19E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.89|P-value:3.27E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.18|P-value:1.06E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:4.75|P-value:5.49E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.90|P-value:2.51E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:-4.54|P-value:7.68E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.79|P-value:5.05E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.61|P-value:7.74E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:-4.53|P-value:7.86E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-6.07|P-value:1.17E-5 ID:SGTC_1191|Compound:1487-1166|FD-Score:-5.76|P-value:3.01E-5 ID:SGTC_1198|Compound:1612-1005|FD-Score:-4.70|P-value:5.26E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:6.26|P-value:8.23E-6 ID:SGTC_1214|Compound:0358-0031|FD-Score:-5.63|P-value:4.42E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.66|P-value:5.76E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.70|P-value:6.32E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:5.32|P-value:1.28E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:-4.49|P-value:8.54E-4 ID:SGTC_1518|Compound:st038567|FD-Score:5.67|P-value:4.83E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.99|P-value:3.05E-4 ID:SGTC_1547|Compound:harmine|FD-Score:7.98|P-value:1.90E-8 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:6.24|P-value:8.84E-6 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.54|P-value:9.15E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.37|P-value:1.94E-7 ID:SGTC_1715|Compound:st032160|FD-Score:-6.80|P-value:1.10E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.83|P-value:4.53E-4 ID:SGTC_1826|Compound:st055655|FD-Score:5.15|P-value:2.00E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.32|P-value:1.28E-4 ID:SGTC_1968|Compound:st069590|FD-Score:-5.10|P-value:1.90E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.61|P-value:7.83E-4 ID:SGTC_2013|Compound:4024628|FD-Score:8.07|P-value:1.37E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:-5.39|P-value:8.68E-5 ID:SGTC_2242|Compound:6630449|FD-Score:9.92|P-value:3.88E-12 ID:SGTC_2264|Compound:7948757|FD-Score:6.05|P-value:1.59E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.08|P-value:1.44E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-5.20|P-value:1.44E-4 ID:SGTC_2332|Compound:7982454|FD-Score:6.45|P-value:4.58E-6 ID:SGTC_2363|Compound:9053361|FD-Score:-4.88|P-value:3.31E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.63|P-value:7.43E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.88|P-value:3.97E-4 ID:SGTC_3027|Compound:9089541|FD-Score:-7.33|P-value:1.69E-7 ID:SGTC_3029|Compound:9088683|FD-Score:-7.67|P-value:4.81E-8 ID:SGTC_3039|Compound:9090270|FD-Score:-7.66|P-value:4.89E-8 ID:SGTC_3125|Compound:9127534|FD-Score:-5.86|P-value:2.25E-5 ID:SGTC_3285|Compound:9112818|FD-Score:4.85|P-value:4.33E-4 ID:SGTC_22|Compound:0964-0034|FD-Score:5.18|P-value:1.87E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:6.52|P-value:3.60E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.87|P-value:4.12E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:4.65|P-value:7.05E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.61|P-value:7.84E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:-4.50|P-value:8.46E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:4.98|P-value:3.13E-4 ID:SGTC_232|Compound:clozapine|FD-Score:4.92|P-value:3.67E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.74|P-value:3.93E-5 ID:SGTC_313|Compound:3474-0040|FD-Score:4.86|P-value:4.18E-4 ID:SGTC_314|Compound:1671-0008|FD-Score:-4.83|P-value:3.74E-4 ID:SGTC_327|Compound:1273-0057|FD-Score:-4.52|P-value:7.96E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:-4.66|P-value:5.80E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:-5.84|P-value:2.33E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:5.22|P-value:1.68E-4 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.58|P-value:6.19E-5 ID:SGTC_526|Compound:4469-0568|FD-Score:-4.89|P-value:3.27E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:6.18|P-value:1.06E-5 ID:SGTC_748|Compound:0136-0228|FD-Score:4.75|P-value:5.49E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.90|P-value:2.51E-5 ID:SGTC_862|Compound:0452-0056|FD-Score:-4.54|P-value:7.68E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:4.79|P-value:5.05E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.61|P-value:7.74E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:-4.53|P-value:7.86E-4 ID:SGTC_989|Compound:1501-0895|FD-Score:-6.07|P-value:1.17E-5 ID:SGTC_1191|Compound:1487-1166|FD-Score:-5.76|P-value:3.01E-5 ID:SGTC_1198|Compound:1612-1005|FD-Score:-4.70|P-value:5.26E-4 ID:SGTC_1202|Compound:1988-0068|FD-Score:6.26|P-value:8.23E-6 ID:SGTC_1214|Compound:0358-0031|FD-Score:-5.63|P-value:4.42E-5 ID:SGTC_1247|Compound:0448-0043|FD-Score:-4.66|P-value:5.76E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.70|P-value:6.32E-4 ID:SGTC_1279|Compound:0874-0590|FD-Score:5.32|P-value:1.28E-4 ID:SGTC_1435|Compound:4092-0789|FD-Score:-4.49|P-value:8.54E-4 ID:SGTC_1518|Compound:st038567|FD-Score:5.67|P-value:4.83E-5 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.99|P-value:3.05E-4 ID:SGTC_1547|Compound:harmine|FD-Score:7.98|P-value:1.90E-8 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:6.24|P-value:8.84E-6 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:4.54|P-value:9.15E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.37|P-value:1.94E-7 ID:SGTC_1715|Compound:st032160|FD-Score:-6.80|P-value:1.10E-6 ID:SGTC_1760|Compound:st045453|FD-Score:4.83|P-value:4.53E-4 ID:SGTC_1826|Compound:st055655|FD-Score:5.15|P-value:2.00E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:5.32|P-value:1.28E-4 ID:SGTC_1968|Compound:st069590|FD-Score:-5.10|P-value:1.90E-4 ID:SGTC_2012|Compound:4023501|FD-Score:4.61|P-value:7.83E-4 ID:SGTC_2013|Compound:4024628|FD-Score:8.07|P-value:1.37E-8 ID:SGTC_2129|Compound:girgensonine|FD-Score:-5.39|P-value:8.68E-5 ID:SGTC_2242|Compound:6630449|FD-Score:9.92|P-value:3.88E-12 ID:SGTC_2264|Compound:7948757|FD-Score:6.05|P-value:1.59E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.08|P-value:1.44E-5 ID:SGTC_2318|Compound:6029776|FD-Score:-5.20|P-value:1.44E-4 ID:SGTC_2332|Compound:7982454|FD-Score:6.45|P-value:4.58E-6 ID:SGTC_2363|Compound:9053361|FD-Score:-4.88|P-value:3.31E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:4.63|P-value:7.43E-4 ID:SGTC_2798|Compound:7813637|FD-Score:4.88|P-value:3.97E-4 ID:SGTC_3027|Compound:9089541|FD-Score:-7.33|P-value:1.69E-7 ID:SGTC_3029|Compound:9088683|FD-Score:-7.67|P-value:4.81E-8 ID:SGTC_3039|Compound:9090270|FD-Score:-7.66|P-value:4.89E-8 ID:SGTC_3125|Compound:9127534|FD-Score:-5.86|P-value:2.25E-5 ID:SGTC_3285|Compound:9112818|FD-Score:4.85|P-value:4.33E-4

Top fitness defect scores for YKR001C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2242 6630449 9.92 61.3 µM 3.88E-12 Golgi 7 1.64E-23
2 SGTC_2013 4024628 8.07 200.0 µM 1.37E-8 6 3.64E-16
3 SGTC_1547 harmine 7.98 94.2 µM 1.90E-8 ergosterol biosynthesis 13 7.10E-16
4 SGTC_1687 st020778 7.37 55.4 µM 1.94E-7 81 8.57E-14
5 SGTC_70 0141-0289 6.52 43.3 µM 3.60E-6 mitochondrial stress 65 3.50E-11
6 SGTC_2332 7982454 6.45 138.9 µM 4.58E-6 14 5.72E-11
7 SGTC_1202 1988-0068 6.26 29.6 µM 8.23E-6 RNA processing & uracil transport 6 1.91E-10
8 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 6.24 67.5 µM 8.84E-6 10 2.20E-10
9 SGTC_528 3492-0019 6.18 172.0 µM 1.06E-5 calcium & mitochondrial duress 117 3.19E-10
10 SGTC_2292 7671323 6.08 168.1 µM 1.44E-5 RNA processing & uracil transport 7 6.00E-10
11 SGTC_2264 7948757 6.05 110.8 µM 1.59E-5 2 7.32E-10
12 SGTC_841 0251-0215 5.90 91.2 µM 2.51E-5 38 1.87E-9
13 SGTC_275 trichlorophene 5.74 5.7 µM 3.93E-5 mitochondrial stress 127 4.67E-9
14 SGTC_1518 st038567 5.67 90.8 µM 4.83E-5 5 7.12E-9
15 SGTC_391 gliotoxin 5.58 8.3 µM 6.19E-5 30 1.18E-8
16 SGTC_1279 0874-0590 5.32 65.2 µM 1.28E-4 11 5.20E-8
17 SGTC_1873 rapamycin 5.32 2.0 nM 1.28E-4 42 5.23E-8
18 SGTC_352 k048-0007 5.22 5.1 µM 1.68E-4 ERAD & cell cycle 44 9.01E-8
19 SGTC_22 0964-0034 5.18 14.5 µM 1.87E-4 unfolded protein response 28 1.12E-7
20 SGTC_1826 st055655 5.15 41.6 µM 2.00E-4 ERG2 21 1.29E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.299 3.99E-70 YGL095C VPS45 hom Protein of the Sec1p/Munc-18 family, essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment
0.235 4.73E-43 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.230 2.54E-41 YOL018C TLG2 hom Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.215 1.88E-36 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.215 3.50E-36 YLR148W PEP3 hom Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
0.215 3.20E-36 YOR036W PEP12 hom Target membrane receptor (t-SNARE) for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
0.203 2.15E-32 YGL054C ERV14 hom COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
0.203 2.04E-32 YDR495C VPS3 hom Component of CORVET tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
0.197 1.99E-30 YDR129C SAC6 hom Fimbrin, actin-bundling protein; cooperates with Scp1p (calponin/transgelin) in the organization and maintenance of the actin cytoskeleton; relocalizes from plasma membrane to cytoplasm upon DNA replication stress
0.193 2.48E-29 YKR019C IRS4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.190 1.97E-28 YLR396C VPS33 hom ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essential for protein sorting, vesicle docking and fusion at the vacuole
0.179 2.15E-25 YDL077C VAM6 hom Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by facilitating guanine nucleotide exchange on small guanosine triphosphatase Ypt7p
0.177 5.79E-25 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.176 1.43E-24 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.173 5.76E-24 YGR167W CLC1 hom Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W