HIPHOP chemogenomics database

YKR007W / MEH1

Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR007W / MEH1

Click on Significant Values for Screen Details

Top fitness defect scores for YKR007W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1844	rapamycin	17.40	4.0 nM	7.52E-61		10	9.80E-68
2	SGTC_56	0957-0365	12.40	83.2 µM	7.02E-32	Golgi	3	2.19E-35
3	SGTC_2177	5811945	11.50	192.0 µM	8.38E-28		1	7.79E-31
4	SGTC_2679	rapamycin	10.00	1.0 nM	1.55E-21		26	7.71E-24
5	SGTC_893	0958-0369	9.49	48.9 µM	1.41E-19		1	1.18E-21
6	SGTC_46	0329-0187a	8.41	2.2 µM	8.77E-16		13	2.02E-17
7	SGTC_1877	wortmannin	7.68	25.0 µM	1.81E-13		24	7.72E-15
8	SGTC_2912	7964298	7.66	10.9 µM	2.13E-13	cell wall	29	9.23E-15
9	SGTC_451	chloral hydrate	7.50	100.0 µM	6.64E-13		8	3.29E-14
10	SGTC_58	0957-0393	6.91	9.0 µM	3.06E-11	cell wall	167	2.35E-12
11	SGTC_2651	1327-0036	5.98	5.0 µM	7.91E-9		74	1.15E-9
12	SGTC_3266	9136860	5.97	49.5 µM	8.22E-9	cell wall	17	1.20E-9
13	SGTC_1781	st038725	5.82	66.3 µM	1.84E-8	NEO1	59	2.94E-9
14	SGTC_2772	vinpocetine	5.77	57.1 µM	2.45E-8		5	4.05E-9
15	SGTC_449	xestospongin c	5.76	11.2 µM	2.56E-8	NEO1-PIK1	21	4.25E-9
16	SGTC_138	0850-0259	5.65	40.9 µM	4.56E-8	cell wall	118	8.09E-9
17	SGTC_2284	7945075	5.33	39.2 µM	2.32E-7	mitochondrial processes	7	4.95E-8
18	SGTC_2264	7948757	5.22	110.8 µM	3.87E-7		4	8.75E-8
19	SGTC_221	trifluoperazine	5.10	10.3 µM	7.05E-7	NEO1-PIK1	154	1.70E-7
20	SGTC_228	caffeine	4.86	993.1 µM	2.13E-6		6	5.84E-7

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.528	8.34E-240	YML121W	GTR1	hom	Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.461	1.67E-175	YBR077C	SLM4	hom	Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.461	1.02E-175	YGR163W	GTR2	hom	Putative GTP binding protein that negatively regulates Ran/Tc4 GTPase cycle; activates transcription; subunit of EGO and GSE complexes; required for sorting of Gap1p; localizes to cytoplasm and to chromatin; homolog of human RagC and RagD
0.340	1.11E-91	YJR066W	TOR1	hom	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.311	2.61E-76	YKL203C	TOR2	het	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.296	5.52E-69	YPL180W	TCO89	hom	Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p), a complex that regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
0.296	9.42E-69	YLL043W	FPS1	hom	Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.292	5.06E-67	YHR186C	KOG1	het	Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.281	5.64E-62	YCR075W-A_p	YCR075W-A_p	hom	Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.278	1.73E-60	YLR114C	AVL9	hom	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.265	4.41E-55	YKL110C	KTI12	hom	Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.243	3.00E-46	YIL052C	RPL34B	hom	Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.237	5.53E-44	YKL176C	LST4	hom	Protein possibly involved in a post-Golgi secretory pathway; required for the transport of nitrogen-regulated amino acid permease Gap1p from the Golgi to the cell surface
0.221	4.36E-38	YNL119W	NCS2	hom	Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.213	1.59E-35	YOR184W	SER1	hom	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress