YKR010C / TOF2

Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR010C / TOF2

Click on Significant Values for Screen Details ID:SGTC_68|Compound:0986-0284|FD-Score:-3.74|P-value:2.59E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.10|P-value:3.99E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:3.74|P-value:2.53E-5 ID:SGTC_355|Compound:1311-2252|FD-Score:2.97|P-value:6.46E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:2.97|P-value:6.45E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.22|P-value:2.40E-4 ID:SGTC_500|Compound:h-89|FD-Score:5.79|P-value:1.82E-10 ID:SGTC_504|Compound:lovastatin|FD-Score:3.49|P-value:7.73E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:5.70|P-value:3.29E-10 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.70|P-value:3.12E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.28|P-value:1.87E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.01|P-value:5.67E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.09|P-value:4.03E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.76|P-value:2.36E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.63|P-value:5.53E-10 ID:SGTC_1149|Compound:3909-7961|FD-Score:-3.83|P-value:1.71E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.38|P-value:1.24E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:2.98|P-value:6.21E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.86|P-value:9.81E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.03|P-value:6.46E-6 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.93|P-value:7.53E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:3.01|P-value:5.58E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.77|P-value:2.21E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.89|P-value:1.27E-5 ID:SGTC_1651|Compound:st011928|FD-Score:3.23|P-value:2.32E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.07|P-value:4.43E-4 ID:SGTC_1920|Compound:st075728|FD-Score:4.51|P-value:5.12E-7 ID:SGTC_1921|Compound:5740136|FD-Score:-5.89|P-value:9.33E-11 ID:SGTC_1922|Compound:5578187|FD-Score:3.81|P-value:1.85E-5 ID:SGTC_1925|Compound:st057770|FD-Score:-3.12|P-value:3.63E-4 ID:SGTC_2064|Compound:5220758|FD-Score:2.97|P-value:6.46E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.49|P-value:7.96E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.89|P-value:1.23E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.92|P-value:5.13E-8 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.08|P-value:5.03E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.29|P-value:1.85E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.40|P-value:1.15E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.51|P-value:7.02E-5 ID:SGTC_2913|Compound:7966556|FD-Score:4.06|P-value:5.41E-6 ID:SGTC_2916|Compound:7875993|FD-Score:3.02|P-value:5.40E-4 ID:SGTC_2929|Compound:9013348|FD-Score:2.95|P-value:6.86E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-2.91|P-value:8.10E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.64|P-value:3.99E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.02|P-value:5.32E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.06|P-value:4.56E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.72|P-value:2.83E-5 ID:SGTC_3352|Compound:9151559|FD-Score:-3.58|P-value:5.41E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:-3.74|P-value:2.59E-5 ID:SGTC_91|Compound:0417-1751|FD-Score:3.41|P-value:1.12E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.10|P-value:3.99E-4 ID:SGTC_328|Compound:k915-0120|FD-Score:3.74|P-value:2.53E-5 ID:SGTC_355|Compound:1311-2252|FD-Score:2.97|P-value:6.46E-4 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:2.97|P-value:6.45E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.22|P-value:2.40E-4 ID:SGTC_500|Compound:h-89|FD-Score:5.79|P-value:1.82E-10 ID:SGTC_504|Compound:lovastatin|FD-Score:3.49|P-value:7.73E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:5.70|P-value:3.29E-10 ID:SGTC_508|Compound:roscovitine|FD-Score:-3.70|P-value:3.12E-5 ID:SGTC_653|Compound:0203-0016|FD-Score:3.28|P-value:1.87E-4 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.01|P-value:5.67E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.09|P-value:4.03E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:3.76|P-value:2.36E-5 ID:SGTC_1129|Compound:3453-1384|FD-Score:5.63|P-value:5.53E-10 ID:SGTC_1149|Compound:3909-7961|FD-Score:-3.83|P-value:1.71E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.38|P-value:1.24E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:2.98|P-value:6.21E-4 ID:SGTC_1253|Compound:0550-0034|FD-Score:2.86|P-value:9.81E-4 ID:SGTC_1263|Compound:0774-1208|FD-Score:-4.03|P-value:6.46E-6 ID:SGTC_1275|Compound:0867-0008|FD-Score:2.93|P-value:7.53E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:3.01|P-value:5.58E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.77|P-value:2.21E-5 ID:SGTC_1604|Compound:st002052|FD-Score:3.89|P-value:1.27E-5 ID:SGTC_1651|Compound:st011928|FD-Score:3.23|P-value:2.32E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.07|P-value:4.43E-4 ID:SGTC_1920|Compound:st075728|FD-Score:4.51|P-value:5.12E-7 ID:SGTC_1921|Compound:5740136|FD-Score:-5.89|P-value:9.33E-11 ID:SGTC_1922|Compound:5578187|FD-Score:3.81|P-value:1.85E-5 ID:SGTC_1925|Compound:st057770|FD-Score:-3.12|P-value:3.63E-4 ID:SGTC_2064|Compound:5220758|FD-Score:2.97|P-value:6.46E-4 ID:SGTC_2327|Compound:6189656|FD-Score:3.49|P-value:7.96E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.89|P-value:1.23E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-4.92|P-value:5.13E-8 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.08|P-value:5.03E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:-3.29|P-value:1.85E-4 ID:SGTC_2708|Compound:st078559|FD-Score:3.40|P-value:1.15E-4 ID:SGTC_2807|Compound:7945238|FD-Score:3.51|P-value:7.02E-5 ID:SGTC_2913|Compound:7966556|FD-Score:4.06|P-value:5.41E-6 ID:SGTC_2916|Compound:7875993|FD-Score:3.02|P-value:5.40E-4 ID:SGTC_2929|Compound:9013348|FD-Score:2.95|P-value:6.86E-4 ID:SGTC_3062|Compound:9077494|FD-Score:-2.91|P-value:8.10E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.64|P-value:3.99E-5 ID:SGTC_3285|Compound:9112818|FD-Score:-3.02|P-value:5.32E-4 ID:SGTC_3337|Compound:9144342|FD-Score:-3.06|P-value:4.56E-4 ID:SGTC_3343|Compound:9148379|FD-Score:3.72|P-value:2.83E-5 ID:SGTC_3352|Compound:9151559|FD-Score:-3.58|P-value:5.41E-5

Top fitness defect scores for YKR010C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_500 h-89 5.79 112.0 µM 1.82E-10 cell wall signaling 12 3.58E-9
2 SGTC_506 n9-isopropylolomoucine 5.70 153.0 µM 3.29E-10 2 5.94E-9
3 SGTC_1129 3453-1384 5.63 223.0 µM 5.53E-10 13 9.25E-9
4 SGTC_1920 st075728 4.51 45.7 µM 5.12E-7 8 3.22E-6
5 SGTC_2594 a-mangostin 4.08 100.0 µM 5.03E-6 29 2.29E-5
6 SGTC_2913 7966556 4.06 36.6 µM 5.41E-6 60S ribosome export 9 2.43E-5
7 SGTC_2402 5553605 3.89 200.0 µM 1.23E-5 30 4.93E-5
8 SGTC_1604 st002052 3.89 72.4 µM 1.27E-5 TSC3-RPN4 32 5.08E-5
9 SGTC_1922 5578187 3.81 25.0 µM 1.85E-5 TRP & mitochondrial translation 38 7.00E-5
10 SGTC_1427 4048-4900 3.77 550.0 nM 2.21E-5 32 8.15E-5
11 SGTC_785 1270-0111 3.76 65.3 µM 2.36E-5 32 8.62E-5
12 SGTC_328 k915-0120 3.74 63.5 µM 2.53E-5 TSC3-RPN4 26 9.17E-5
13 SGTC_3343 9148379 3.72 19.0 µM 2.83E-5 6 1.01E-4
14 SGTC_3106 9121286 3.64 49.5 µM 3.99E-5 20 1.35E-4
15 SGTC_2807 7945238 3.51 52.0 µM 7.02E-5 40 2.20E-4
16 SGTC_504 lovastatin 3.49 124.0 µM 7.73E-5 20 2.39E-4
17 SGTC_2327 6189656 3.49 200.0 µM 7.96E-5 34 2.45E-4
18 SGTC_91 0417-1751 3.41 58.4 µM 1.12E-4 Golgi 73 3.28E-4
19 SGTC_2708 st078559 3.40 56.0 µM 1.15E-4 16 3.37E-4
20 SGTC_1233 0325-0408 3.38 14.2 µM 1.24E-4 40 3.59E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.150 2.46E-18 YHR110W ERP5 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.110 1.83E-10 YLR276C DBP9 het DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
0.104 1.48E-9 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.097 1.90E-8 YIL019W FAF1 het Protein required for pre-rRNA processing and 40S ribosomal subunit assembly
0.095 3.36E-8 YGR074W SMD1 het Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
0.095 3.46E-8 YJR022W LSM8 het Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
0.095 3.54E-8 YIL037C PRM2 hom Pheromone-regulated protein, predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
0.093 7.67E-8 YGR143W SKN1 hom Protein involved in sphingolipid biosynthesis; type II membrane protein with similarity to Kre6p
0.085 7.11E-7 YGL260W_p YGL260W_p hom Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
0.083 1.38E-6 YDR020C_p DAS2_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
0.077 7.17E-6 YOR343C_d YOR343C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.076 9.61E-6 YLR363W-A YLR363W-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
0.074 1.74E-5 YDR125C ECM18 hom Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
0.073 2.49E-5 YLL030C_d RRT7_d hom Identified in a screen for mutants with increased levels of rDNA transcription; dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.072 2.73E-5 YOR376W_d YOR376W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YOR376W is not an essential gene.