YKR020W / VPS51

Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR020W / VPS51

Click on Significant Values for Screen Details ID:SGTC_22|Compound:0964-0034|FD-Score:4.52|P-value:7.43E-5 ID:SGTC_57|Compound:miconazole|FD-Score:-6.78|P-value:6.88E-9 ID:SGTC_174|Compound:3553-0389|FD-Score:4.95|P-value:1.68E-5 ID:SGTC_176|Compound:1486-1328|FD-Score:5.44|P-value:2.58E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.06|P-value:3.37E-4 ID:SGTC_232|Compound:clozapine|FD-Score:3.98|P-value:4.23E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.70|P-value:9.65E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.15|P-value:2.50E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.10|P-value:2.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.40|P-value:4.10E-8 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.72|P-value:9.27E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.35|P-value:3.57E-6 ID:SGTC_489|Compound:paxilline|FD-Score:3.79|P-value:7.45E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-4.29|P-value:1.65E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.50|P-value:7.99E-5 ID:SGTC_591|Compound:4299-0174|FD-Score:4.10|P-value:2.94E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.85|P-value:6.15E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:5.56|P-value:1.58E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:-4.51|P-value:8.07E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:4.85|P-value:2.39E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:-4.13|P-value:2.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.42|P-value:1.06E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.77|P-value:3.16E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:5.17|P-value:7.32E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:6.04|P-value:2.02E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.92|P-value:5.01E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.12|P-value:2.72E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.83|P-value:6.61E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.78|P-value:7.63E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:6.80|P-value:6.03E-9 ID:SGTC_1377|Compound:2944-0135|FD-Score:4.38|P-value:1.20E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.90|P-value:2.00E-5 ID:SGTC_1507|Compound:4075-3230|FD-Score:-4.94|P-value:1.75E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.16|P-value:2.44E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.90|P-value:5.51E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.47|P-value:8.82E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-4.28|P-value:1.68E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.54|P-value:7.03E-5 ID:SGTC_1606|Compound:st000094|FD-Score:-3.71|P-value:9.51E-4 ID:SGTC_1622|Compound:st003707|FD-Score:5.55|P-value:1.63E-6 ID:SGTC_1623|Compound:st003709|FD-Score:5.10|P-value:9.65E-6 ID:SGTC_1726|Compound:st035556|FD-Score:-4.65|P-value:4.87E-5 ID:SGTC_1885|Compound:st057546|FD-Score:-3.90|P-value:5.43E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.72|P-value:9.31E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.93|P-value:4.91E-4 ID:SGTC_1963|Compound:st076565|FD-Score:4.72|P-value:3.74E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.07|P-value:3.21E-4 ID:SGTC_1975|Compound:st070967|FD-Score:5.48|P-value:2.13E-6 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:4.10|P-value:2.89E-4 ID:SGTC_2188|Compound:6126565|FD-Score:4.04|P-value:3.47E-4 ID:SGTC_2201|Compound:6605497|FD-Score:-4.29|P-value:1.62E-4 ID:SGTC_2223|Compound:6903751|FD-Score:-3.78|P-value:7.86E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.53|P-value:7.31E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.97|P-value:4.35E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-4.23|P-value:1.98E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.00|P-value:3.96E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:6.83|P-value:5.19E-9 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.79|P-value:7.49E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.75|P-value:8.40E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.78|P-value:7.71E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.31|P-value:1.53E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.42|P-value:1.06E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.75|P-value:8.31E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.78|P-value:6.42E-9 ID:SGTC_2782|Compound:7736369|FD-Score:4.37|P-value:1.22E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.86|P-value:5.94E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.63|P-value:5.33E-5 ID:SGTC_3299|Compound:9121982|FD-Score:4.11|P-value:2.81E-4 ID:SGTC_3308|Compound:9128332|FD-Score:-5.24|P-value:5.74E-6 ID:SGTC_22|Compound:0964-0034|FD-Score:4.52|P-value:7.43E-5 ID:SGTC_57|Compound:miconazole|FD-Score:-6.78|P-value:6.88E-9 ID:SGTC_174|Compound:3553-0389|FD-Score:4.95|P-value:1.68E-5 ID:SGTC_176|Compound:1486-1328|FD-Score:5.44|P-value:2.58E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-4.06|P-value:3.37E-4 ID:SGTC_232|Compound:clozapine|FD-Score:3.98|P-value:4.23E-4 ID:SGTC_396|Compound:dithiazanine|FD-Score:3.70|P-value:9.65E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:4.15|P-value:2.50E-4 ID:SGTC_449|Compound:xestospongin c|FD-Score:4.10|P-value:2.93E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.40|P-value:4.10E-8 ID:SGTC_455|Compound:paf C-16|FD-Score:-3.72|P-value:9.27E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:5.35|P-value:3.57E-6 ID:SGTC_489|Compound:paxilline|FD-Score:3.79|P-value:7.45E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:-4.29|P-value:1.65E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:4.50|P-value:7.99E-5 ID:SGTC_591|Compound:4299-0174|FD-Score:4.10|P-value:2.94E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.85|P-value:6.15E-4 ID:SGTC_772|Compound:k072-0172|FD-Score:5.56|P-value:1.58E-6 ID:SGTC_775|Compound:4577-0580|FD-Score:-4.51|P-value:8.07E-5 ID:SGTC_777|Compound:1315-0167|FD-Score:4.85|P-value:2.39E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:-4.13|P-value:2.75E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:4.42|P-value:1.06E-4 ID:SGTC_893|Compound:0958-0369|FD-Score:4.77|P-value:3.16E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:5.17|P-value:7.32E-6 ID:SGTC_1098|Compound:clofazimine|FD-Score:6.04|P-value:2.02E-7 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.92|P-value:5.01E-4 ID:SGTC_1155|Compound:4088-0394|FD-Score:4.12|P-value:2.72E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.83|P-value:6.61E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.78|P-value:7.63E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:6.80|P-value:6.03E-9 ID:SGTC_1377|Compound:2944-0135|FD-Score:4.38|P-value:1.20E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.90|P-value:2.00E-5 ID:SGTC_1507|Compound:4075-3230|FD-Score:-4.94|P-value:1.75E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.16|P-value:2.44E-4 ID:SGTC_1547|Compound:harmine|FD-Score:-3.90|P-value:5.51E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:4.47|P-value:8.82E-5 ID:SGTC_1573|Compound:oleylamine|FD-Score:-4.28|P-value:1.68E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.54|P-value:7.03E-5 ID:SGTC_1606|Compound:st000094|FD-Score:-3.71|P-value:9.51E-4 ID:SGTC_1622|Compound:st003707|FD-Score:5.55|P-value:1.63E-6 ID:SGTC_1623|Compound:st003709|FD-Score:5.10|P-value:9.65E-6 ID:SGTC_1726|Compound:st035556|FD-Score:-4.65|P-value:4.87E-5 ID:SGTC_1885|Compound:st057546|FD-Score:-3.90|P-value:5.43E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.72|P-value:9.31E-4 ID:SGTC_1959|Compound:st077091|FD-Score:3.93|P-value:4.91E-4 ID:SGTC_1963|Compound:st076565|FD-Score:4.72|P-value:3.74E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.07|P-value:3.21E-4 ID:SGTC_1975|Compound:st070967|FD-Score:5.48|P-value:2.13E-6 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:4.10|P-value:2.89E-4 ID:SGTC_2188|Compound:6126565|FD-Score:4.04|P-value:3.47E-4 ID:SGTC_2201|Compound:6605497|FD-Score:-4.29|P-value:1.62E-4 ID:SGTC_2223|Compound:6903751|FD-Score:-3.78|P-value:7.86E-4 ID:SGTC_2293|Compound:7624732|FD-Score:4.53|P-value:7.31E-5 ID:SGTC_2401|Compound:5128683|FD-Score:3.97|P-value:4.35E-4 ID:SGTC_2416|Compound:tofa|FD-Score:-4.23|P-value:1.98E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:4.00|P-value:3.96E-4 ID:SGTC_2495|Compound:cafestol|FD-Score:6.83|P-value:5.19E-9 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.79|P-value:7.49E-4 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:3.75|P-value:8.40E-4 ID:SGTC_2507|Compound:purpurin|FD-Score:3.78|P-value:7.71E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.31|P-value:1.53E-4 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.42|P-value:1.06E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:3.75|P-value:8.31E-4 ID:SGTC_2737|Compound:bleomycin|FD-Score:6.78|P-value:6.42E-9 ID:SGTC_2782|Compound:7736369|FD-Score:4.37|P-value:1.22E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.86|P-value:5.94E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.63|P-value:5.33E-5 ID:SGTC_3299|Compound:9121982|FD-Score:4.11|P-value:2.81E-4 ID:SGTC_3308|Compound:9128332|FD-Score:-5.24|P-value:5.74E-6

Top fitness defect scores for YKR020W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2495 cafestol 6.83 54.5 µM 5.19E-9 Golgi 5 4.32E-12
2 SGTC_1327 1436-0082 6.80 2.2 µM 6.03E-9 4 5.35E-12
3 SGTC_2737 bleomycin 6.78 30.5 nM 6.42E-9 Golgi 26 5.84E-12
4 SGTC_1098 clofazimine 6.04 15.1 µM 2.02E-7 heme biosynthesis & mitochondrial translocase 4 7.55E-10
5 SGTC_772 k072-0172 5.56 35.3 µM 1.58E-6 2 1.37E-8
6 SGTC_1622 st003707 5.55 19.4 µM 1.63E-6 calcium & mitochondrial duress 11 1.43E-8
7 SGTC_1975 st070967 5.48 30.8 µM 2.13E-6 12 2.09E-8
8 SGTC_176 1486-1328 5.44 72.8 µM 2.58E-6 29 2.72E-8
9 SGTC_467 ro 31-8220 5.35 72.8 µM 3.57E-6 calcium & mitochondrial duress 39 4.31E-8
10 SGTC_1022 4477-0945 5.17 21.3 µM 7.32E-6 7 1.18E-7
11 SGTC_1623 st003709 5.10 20.3 µM 9.65E-6 calcium & mitochondrial duress 28 1.74E-7
12 SGTC_174 3553-0389 4.95 74.3 µM 1.68E-5 7 3.80E-7
13 SGTC_1495 4469-0029 4.90 237.0 µM 2.00E-5 75 4.85E-7
14 SGTC_777 1315-0167 4.85 517.0 µM 2.39E-5 Golgi 16 6.21E-7
15 SGTC_893 0958-0369 4.77 48.9 µM 3.16E-5 14 9.20E-7
16 SGTC_1963 st076565 4.72 50.3 µM 3.74E-5 2 1.17E-6
17 SGTC_1597 7-benzyloxygramine 4.54 47.5 µM 7.03E-5 calcium & mitochondrial duress 83 2.82E-6
18 SGTC_2293 7624732 4.53 200.0 µM 7.31E-5 15 2.98E-6
19 SGTC_22 0964-0034 4.52 14.5 µM 7.43E-5 unfolded protein response 34 3.05E-6
20 SGTC_528 3492-0019 4.50 172.0 µM 7.99E-5 calcium & mitochondrial duress 180 3.38E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.238 3.33E-44 YOL018C TLG2 hom Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.197 1.22E-30 YDR137W RGP1 hom Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
0.178 2.45E-25 YDR136C_d VPS61_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
0.176 8.98E-25 YLR261C_d VPS63_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
0.176 1.13E-24 YKR019C IRS4 hom EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.175 1.91E-24 YLR262C YPT6 hom Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
0.161 8.45E-21 YDR484W VPS52 hom Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
0.159 2.27E-20 YGL005C COG7 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.159 2.11E-20 YGR285C ZUO1 hom Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.157 6.27E-20 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.156 1.41E-19 YNL051W COG5 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.151 1.50E-18 YLR039C RIC1 hom Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
0.151 1.66E-18 YDR320C SWA2 hom Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
0.148 7.75E-18 YML071C COG8 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.147 9.11E-18 YNL041C COG6 hom Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments