HIPHOP chemogenomics database

YKR020W / VPS51

Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR020W / VPS51

Click on Significant Values for Screen Details

Top fitness defect scores for YKR020W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_2495	cafestol	6.83	54.5 µM	5.19E-9	Golgi	5	4.32E-12
2	SGTC_1327	1436-0082	6.80	2.2 µM	6.03E-9		4	5.35E-12
3	SGTC_2737	bleomycin	6.78	30.5 nM	6.42E-9	Golgi	26	5.84E-12
4	SGTC_1098	clofazimine	6.04	15.1 µM	2.02E-7	heme biosynthesis & mitochondrial translocase	4	7.55E-10
5	SGTC_772	k072-0172	5.56	35.3 µM	1.58E-6		2	1.37E-8
6	SGTC_1622	st003707	5.55	19.4 µM	1.63E-6	calcium & mitochondrial duress	11	1.43E-8
7	SGTC_1975	st070967	5.48	30.8 µM	2.13E-6		12	2.09E-8
8	SGTC_176	1486-1328	5.44	72.8 µM	2.58E-6		29	2.72E-8
9	SGTC_467	ro 31-8220	5.35	72.8 µM	3.57E-6	calcium & mitochondrial duress	39	4.31E-8
10	SGTC_1022	4477-0945	5.17	21.3 µM	7.32E-6		7	1.18E-7
11	SGTC_1623	st003709	5.10	20.3 µM	9.65E-6	calcium & mitochondrial duress	28	1.74E-7
12	SGTC_174	3553-0389	4.95	74.3 µM	1.68E-5		7	3.80E-7
13	SGTC_1495	4469-0029	4.90	237.0 µM	2.00E-5		75	4.85E-7
14	SGTC_777	1315-0167	4.85	517.0 µM	2.39E-5	Golgi	16	6.21E-7
15	SGTC_893	0958-0369	4.77	48.9 µM	3.16E-5		14	9.20E-7
16	SGTC_1963	st076565	4.72	50.3 µM	3.74E-5		2	1.17E-6
17	SGTC_1597	7-benzyloxygramine	4.54	47.5 µM	7.03E-5	calcium & mitochondrial duress	83	2.82E-6
18	SGTC_2293	7624732	4.53	200.0 µM	7.31E-5		15	2.98E-6
19	SGTC_22	0964-0034	4.52	14.5 µM	7.43E-5	unfolded protein response	34	3.05E-6
20	SGTC_528	3492-0019	4.50	172.0 µM	7.99E-5	calcium & mitochondrial duress	180	3.38E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.238	3.33E-44	YOL018C	TLG2	hom	Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.197	1.22E-30	YDR137W	RGP1	hom	Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
0.178	2.45E-25	YDR136C_d	VPS61_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
0.176	8.98E-25	YLR261C_d	VPS63_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
0.176	1.13E-24	YKR019C	IRS4	hom	EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.175	1.91E-24	YLR262C	YPT6	hom	Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
0.161	8.45E-21	YDR484W	VPS52	hom	Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; involved in localization of actin and chitin
0.159	2.27E-20	YGL005C	COG7	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.159	2.11E-20	YGR285C	ZUO1	hom	Ribosome-associated chaperone, functions in ribosome biogenesis and, in partnership with Ssz1p and SSb1/2, as a chaperone for nascent polypeptide chains; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p
0.157	6.27E-20	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.156	1.41E-19	YNL051W	COG5	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.151	1.50E-18	YLR039C	RIC1	hom	Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
0.151	1.66E-18	YDR320C	SWA2	hom	Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
0.148	7.75E-18	YML071C	COG8	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.147	9.11E-18	YNL041C	COG6	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments