YKR021W / ALY1

Alpha arrestin that controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR021W / ALY1

Click on Significant Values for Screen Details ID:SGTC_155|Compound:k072-0202|FD-Score:3.07|P-value:8.14E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.30|P-value:3.33E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.67|P-value:8.20E-5 ID:SGTC_621|Compound:3584-0052|FD-Score:3.27|P-value:3.93E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.24|P-value:4.45E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-4.32|P-value:4.31E-6 ID:SGTC_779|Compound:1360-0071|FD-Score:4.32|P-value:4.57E-6 ID:SGTC_807|Compound:1315-0151|FD-Score:4.21|P-value:7.57E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:1.99E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.52|P-value:1.54E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.11|P-value:6.72E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.31|P-value:4.82E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.16|P-value:8.96E-6 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.59|P-value:1.13E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.34|P-value:2.90E-4 ID:SGTC_1649|Compound:st011737|FD-Score:5.64|P-value:3.37E-9 ID:SGTC_1672|Compound:st016598|FD-Score:3.36|P-value:2.83E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.22|P-value:4.68E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:4.77|P-value:4.72E-7 ID:SGTC_1945|Compound:st075991|FD-Score:3.06|P-value:8.31E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.53|P-value:1.47E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.50|P-value:1.55E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.56|P-value:1.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.31|P-value:3.37E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.02|P-value:9.39E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.10|P-value:1.31E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.07|P-value:8.25E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.10|P-value:6.93E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.05|P-value:8.76E-4 ID:SGTC_2525|Compound:harmine|FD-Score:6.18|P-value:1.07E-10 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.68|P-value:8.00E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:-3.40|P-value:2.33E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:4.07|P-value:1.46E-5 ID:SGTC_3128|Compound:9127691|FD-Score:3.34|P-value:3.02E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.39|P-value:2.52E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.71|P-value:7.08E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:3.07|P-value:8.14E-4 ID:SGTC_450|Compound:aminopyrene|FD-Score:-3.30|P-value:3.33E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.67|P-value:8.20E-5 ID:SGTC_621|Compound:3584-0052|FD-Score:3.27|P-value:3.93E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.24|P-value:4.45E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-4.32|P-value:4.31E-6 ID:SGTC_779|Compound:1360-0071|FD-Score:4.32|P-value:4.57E-6 ID:SGTC_807|Compound:1315-0151|FD-Score:4.21|P-value:7.57E-6 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:1.99E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.52|P-value:1.54E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:-3.11|P-value:6.72E-4 ID:SGTC_1393|Compound:3470-5649|FD-Score:4.31|P-value:4.82E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.16|P-value:8.96E-6 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.59|P-value:1.13E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.34|P-value:2.90E-4 ID:SGTC_1649|Compound:st011737|FD-Score:5.64|P-value:3.37E-9 ID:SGTC_1672|Compound:st016598|FD-Score:3.36|P-value:2.83E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.22|P-value:4.68E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:4.77|P-value:4.72E-7 ID:SGTC_1945|Compound:st075991|FD-Score:3.06|P-value:8.31E-4 ID:SGTC_1998|Compound:st075797|FD-Score:3.53|P-value:1.47E-4 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:-3.50|P-value:1.55E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.56|P-value:1.30E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.31|P-value:3.37E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.26|P-value:4.07E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.02|P-value:9.39E-4 ID:SGTC_2413|Compound:inermin|FD-Score:4.10|P-value:1.31E-5 ID:SGTC_2437|Compound:5741531|FD-Score:3.07|P-value:8.25E-4 ID:SGTC_2514|Compound:salinomycin|FD-Score:-3.10|P-value:6.93E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.05|P-value:8.76E-4 ID:SGTC_2525|Compound:harmine|FD-Score:6.18|P-value:1.07E-10 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.68|P-value:8.00E-5 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:-3.40|P-value:2.33E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:4.07|P-value:1.46E-5 ID:SGTC_3128|Compound:9127691|FD-Score:3.34|P-value:3.02E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.39|P-value:2.52E-4 ID:SGTC_3346|Compound:9158254|FD-Score:3.71|P-value:7.08E-5

Top fitness defect scores for YKR021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2525 harmine 6.18 47.9 µM 1.07E-10 ergosterol biosynthesis 4 3.15E-10
2 SGTC_1649 st011737 5.64 51.3 µM 3.37E-9 16 8.30E-9
3 SGTC_1869 3',7'-dimethoxyflavone 4.77 40.3 µM 4.72E-7 9 9.01E-7
4 SGTC_779 1360-0071 4.32 233.0 µM 4.57E-6 fatty acid desaturase (OLE1) 21 7.76E-6
5 SGTC_1393 3470-5649 4.31 58.2 µM 4.82E-6 60S ribosome export 10 8.16E-6
6 SGTC_807 1315-0151 4.21 48.0 µM 7.57E-6 20 1.25E-5
7 SGTC_2413 inermin 4.10 26.1 µM 1.31E-5 48 2.11E-5
8 SGTC_2721 ifenprodil 4.07 61.4 µM 1.46E-5 plasma membrane duress 14 2.33E-5
9 SGTC_3346 9158254 3.71 43.6 µM 7.08E-5 24 1.05E-4
10 SGTC_2588 indole-3-carbinol 3.68 100.0 µM 8.00E-5 57 1.18E-4
11 SGTC_620 0109-0052 3.67 432.0 µM 8.20E-5 26 1.20E-4
12 SGTC_1494 4358-1428 3.59 177.0 µM 1.13E-4 32 1.63E-4
13 SGTC_2124 5246320 3.56 200.0 µM 1.30E-4 heme biosynthesis & mitochondrial translocase 28 1.87E-4
14 SGTC_1998 st075797 3.53 76.8 µM 1.47E-4 mitochondrial response to ROS 27 2.10E-4
15 SGTC_1061 3448-9325 3.52 110.0 µM 1.54E-4 33 2.19E-4
16 SGTC_928 2764-0428 3.45 58.7 µM 1.99E-4 cell wall 81 2.80E-4
17 SGTC_3310 9130229 3.39 44.1 µM 2.52E-4 Golgi 46 3.49E-4
18 SGTC_1672 st016598 3.36 6.5 µM 2.83E-4 endomembrane recycling 93 3.91E-4
19 SGTC_3128 9127691 3.34 49.5 µM 3.02E-4 34 4.15E-4
20 SGTC_2336 9001819 3.31 198.7 µM 3.37E-4 heme biosynthesis & mitochondrial translocase 79 4.62E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.149 4.39E-18 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.113 6.46E-11 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.105 9.84E-10 YPR130C_d YPR130C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.096 2.85E-8 YLR457C NBP1 het Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
0.090 1.59E-7 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.089 2.39E-7 YPR188C MLC2 hom Regulatory light chain for the type II myosin, Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
0.089 2.96E-7 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.085 7.88E-7 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.084 1.02E-6 YER001W MNN1 hom Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides, one of five S. cerevisiae proteins of the MNN1 family
0.084 1.05E-6 YFL051C_p YFL051C_p hom Putative protein of unknown function; YFL051C is not an essential gene
0.084 1.15E-6 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.083 1.29E-6 YBR243C ALG7 het UDP-N-acetyl-glucosamine-1-P transferase, transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin
0.083 1.33E-6 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.079 4.68E-6 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.078 5.76E-6 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts