YKR031C / SPO14

Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR031C / SPO14

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:3.52|P-value:1.48E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.36|P-value:2.82E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.01|P-value:1.27E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:-4.55|P-value:1.38E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.55|P-value:1.26E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:7.98|P-value:1.10E-16 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.06|P-value:8.49E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:4.93|P-value:2.05E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:3.62|P-value:1.01E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.22|P-value:4.70E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.83|P-value:3.97E-5 ID:SGTC_427|Compound:1354-0053|FD-Score:-3.52|P-value:1.45E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.08|P-value:7.66E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.99|P-value:2.03E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:10.30|P-value:1.05E-26 ID:SGTC_568|Compound:1193-0097|FD-Score:9.48|P-value:9.14E-23 ID:SGTC_637|Compound:1181-0519|FD-Score:6.55|P-value:8.19E-12 ID:SGTC_649|Compound:1123-0311|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.88|P-value:3.31E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:3.01|P-value:9.90E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.05|P-value:8.79E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.43|P-value:2.15E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:3.13|P-value:6.44E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.68|P-value:7.75E-5 ID:SGTC_1092|Compound:toremifene|FD-Score:4.61|P-value:1.07E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.26|P-value:4.04E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.63|P-value:9.90E-7 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.87|P-value:3.54E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.76|P-value:5.59E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.64|P-value:8.75E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:-3.58|P-value:1.13E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.28|P-value:3.81E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.50|P-value:1.62E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:16.20|P-value:6.42E-63 ID:SGTC_1726|Compound:st035556|FD-Score:3.08|P-value:7.86E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.17|P-value:5.55E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.07|P-value:7.96E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.58|P-value:1.13E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.91|P-value:2.84E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.04|P-value:8.99E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.17|P-value:5.56E-4 ID:SGTC_2815|Compound:7968870|FD-Score:4.03|P-value:1.71E-5 ID:SGTC_2896|Compound:9052656|FD-Score:7.13|P-value:1.11E-13 ID:SGTC_3050|Compound:9093513|FD-Score:8.28|P-value:8.56E-18 ID:SGTC_3122|Compound:9126309|FD-Score:3.43|P-value:2.12E-4 ID:SGTC_3149|Compound:9097855|FD-Score:16.60|P-value:2.28E-65 ID:SGTC_3152|Compound:9099253|FD-Score:13.30|P-value:9.07E-43 ID:SGTC_3189|Compound:9110682|FD-Score:3.84|P-value:4.02E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.08|P-value:7.82E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.84|P-value:3.90E-5 ID:SGTC_3213|Compound:9129884|FD-Score:-6.86|P-value:8.00E-13 ID:SGTC_3219|Compound:9131112|FD-Score:38.70|P-value:0 ID:SGTC_3221|Compound:9128209|FD-Score:-3.83|P-value:3.93E-5 ID:SGTC_3309|Compound:9129340|FD-Score:3.66|P-value:8.59E-5 ID:SGTC_49|Compound:0717-0965|FD-Score:3.52|P-value:1.48E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:3.36|P-value:2.82E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.01|P-value:1.27E-7 ID:SGTC_72|Compound:0744-0168|FD-Score:-4.55|P-value:1.38E-6 ID:SGTC_73|Compound:0327-0325|FD-Score:-3.55|P-value:1.26E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:7.98|P-value:1.10E-16 ID:SGTC_250|Compound:brefeldin a|FD-Score:3.06|P-value:8.49E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:4.93|P-value:2.05E-7 ID:SGTC_310|Compound:1273-0058|FD-Score:3.62|P-value:1.01E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.22|P-value:4.70E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:-3.83|P-value:3.97E-5 ID:SGTC_427|Compound:1354-0053|FD-Score:-3.52|P-value:1.45E-4 ID:SGTC_455|Compound:paf C-16|FD-Score:3.08|P-value:7.66E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.99|P-value:2.03E-5 ID:SGTC_514|Compound:4466-0038|FD-Score:10.30|P-value:1.05E-26 ID:SGTC_568|Compound:1193-0097|FD-Score:9.48|P-value:9.14E-23 ID:SGTC_637|Compound:1181-0519|FD-Score:6.55|P-value:8.19E-12 ID:SGTC_649|Compound:1123-0311|FD-Score:3.57|P-value:1.24E-4 ID:SGTC_683|Compound:panaxydol|FD-Score:3.88|P-value:3.31E-5 ID:SGTC_813|Compound:k007-0158|FD-Score:3.01|P-value:9.90E-4 ID:SGTC_857|Compound:0438-0306|FD-Score:3.05|P-value:8.79E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.43|P-value:2.15E-4 ID:SGTC_957|Compound:1187-1561|FD-Score:3.13|P-value:6.44E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:3.68|P-value:7.75E-5 ID:SGTC_1092|Compound:toremifene|FD-Score:4.61|P-value:1.07E-6 ID:SGTC_1108|Compound:betulinic acid|FD-Score:3.26|P-value:4.04E-4 ID:SGTC_1124|Compound:fisetin|FD-Score:4.63|P-value:9.90E-7 ID:SGTC_1229|Compound:0139-0193|FD-Score:3.87|P-value:3.54E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.76|P-value:5.59E-5 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.64|P-value:8.75E-5 ID:SGTC_1233|Compound:0325-0408|FD-Score:-3.58|P-value:1.13E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.28|P-value:3.81E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.44|P-value:2.01E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:3.50|P-value:1.62E-4 ID:SGTC_1679|Compound:4130-1276|FD-Score:16.20|P-value:6.42E-63 ID:SGTC_1726|Compound:st035556|FD-Score:3.08|P-value:7.86E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.17|P-value:5.55E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.07|P-value:7.96E-4 ID:SGTC_2274|Compound:7947094|FD-Score:-3.58|P-value:1.13E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:-3.91|P-value:2.84E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.04|P-value:8.99E-4 ID:SGTC_2780|Compound:5215298|FD-Score:3.17|P-value:5.56E-4 ID:SGTC_2815|Compound:7968870|FD-Score:4.03|P-value:1.71E-5 ID:SGTC_2896|Compound:9052656|FD-Score:7.13|P-value:1.11E-13 ID:SGTC_3050|Compound:9093513|FD-Score:8.28|P-value:8.56E-18 ID:SGTC_3122|Compound:9126309|FD-Score:3.43|P-value:2.12E-4 ID:SGTC_3149|Compound:9097855|FD-Score:16.60|P-value:2.28E-65 ID:SGTC_3152|Compound:9099253|FD-Score:13.30|P-value:9.07E-43 ID:SGTC_3189|Compound:9110682|FD-Score:3.84|P-value:4.02E-5 ID:SGTC_3193|Compound:9111931|FD-Score:3.08|P-value:7.82E-4 ID:SGTC_3196|Compound:9111891|FD-Score:3.84|P-value:3.90E-5 ID:SGTC_3213|Compound:9129884|FD-Score:-6.86|P-value:8.00E-13 ID:SGTC_3219|Compound:9131112|FD-Score:38.70|P-value:0 ID:SGTC_3221|Compound:9128209|FD-Score:-3.83|P-value:3.93E-5 ID:SGTC_3309|Compound:9129340|FD-Score:3.66|P-value:8.59E-5

Top fitness defect scores for YKR031C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3219 9131112 38.70 49.5 µM 0 Golgi 1 0
2 SGTC_3149 9097855 16.60 49.5 µM 2.28E-65 Golgi 1 7.21E-62
3 SGTC_1679 4130-1276 16.20 6.7 µM 6.42E-63 Golgi 1 1.48E-59
4 SGTC_3152 9099253 13.30 49.5 µM 9.07E-43 NEO1-PIK1 10 1.60E-40
5 SGTC_514 4466-0038 10.30 2.6 nM 1.05E-26 cell wall 17 2.41E-25
6 SGTC_568 1193-0097 9.48 7.5 µM 9.14E-23 ERAD & cell cycle 27 1.27E-21
7 SGTC_3050 9093513 8.28 49.5 µM 8.56E-18 1 6.37E-17
8 SGTC_207 4185-0010 7.98 4.5 µM 1.10E-16 endomembrane recycling 26 7.12E-16
9 SGTC_2896 9052656 7.13 71.4 µM 1.11E-13 endomembrane recycling 26 4.91E-13
10 SGTC_637 1181-0519 6.55 7.3 µM 8.19E-12 fatty acid desaturase (OLE1) 46 2.88E-11
11 SGTC_63 0099-0242 5.01 26.7 µM 1.27E-7 mitochondrial stress 55 2.65E-7
12 SGTC_299 1273-0059 4.93 1.4 µM 2.05E-7 16 4.19E-7
13 SGTC_1124 fisetin 4.63 1.2 µM 9.90E-7 17 1.86E-6
14 SGTC_1092 toremifene 4.61 6.3 µM 1.07E-6 13 2.00E-6
15 SGTC_2815 7968870 4.03 71.4 µM 1.71E-5 20 2.76E-5
16 SGTC_513 4245-1575 3.99 41.7 µM 2.03E-5 unfolded protein response 116 3.25E-5
17 SGTC_683 panaxydol 3.88 45.0 µM 3.31E-5 sphingolipid biosynthesis & PDR1 42 5.17E-5
18 SGTC_1229 0139-0193 3.87 12.2 µM 3.54E-5 RSC complex & mRNA processing 11 5.51E-5
19 SGTC_3196 9111891 3.84 49.5 µM 3.90E-5 mitochondrial processes 17 6.04E-5
20 SGTC_3189 9110682 3.84 49.5 µM 4.02E-5 40 6.22E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.527 5.45E-239 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.370 1.07E-109 YDL133W SRF1 hom Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
0.244 1.19E-46 YLR080W EMP46 hom Integral membrane component of endoplasmic reticulum-derived COPII-coated vesicles, which function in ER to Golgi transport
0.196 2.02E-30 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.191 5.60E-29 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.185 3.96E-27 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.179 1.83E-25 YCR020C-A MAK31 hom Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
0.177 3.98E-25 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.173 6.53E-24 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.172 1.27E-23 YGR289C MAL11 hom Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
0.169 4.93E-23 YLL049W LDB18 hom Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
0.164 1.13E-21 YDR526C_d YDR526C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.158 3.44E-20 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.157 5.80E-20 YKL039W PTM1 hom Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
0.154 2.89E-19 YDR108W TRS85 hom Subunit of TRAPPIII (transport protein particle), a multimeric guanine nucleotide-exchange factor for Ypt1p, required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role