YKR035C_d / OPI8

Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR035C / OPI8

Click on Significant Values for Screen Details ID:SGTC_131|Compound:0141-0277|FD-Score:3.76|P-value:6.35E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:7.14|P-value:4.11E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:3.76|P-value:6.49E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:4.41|P-value:7.85E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.03|P-value:2.78E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.81|P-value:5.53E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.84|P-value:4.26E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.42|P-value:1.65E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:4.78|P-value:2.09E-5 ID:SGTC_619|Compound:1652-1634|FD-Score:5.28|P-value:2.87E-6 ID:SGTC_776|Compound:4476-4378|FD-Score:3.68|P-value:8.22E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-7.37|P-value:8.87E-11 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.95|P-value:3.00E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.68|P-value:8.08E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.02|P-value:2.87E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.60|P-value:3.93E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:-3.76|P-value:5.38E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.88|P-value:1.40E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:4.24|P-value:1.41E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.65|P-value:8.95E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:4.86|P-value:1.53E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:9.88|P-value:9.31E-18 ID:SGTC_1658|Compound:st012944|FD-Score:3.72|P-value:7.25E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.14|P-value:1.94E-4 ID:SGTC_1693|Compound:xanthone|FD-Score:3.71|P-value:7.52E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-4.16|P-value:1.53E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.87|P-value:2.35E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.50|P-value:1.59E-23 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.07|P-value:9.28E-8 ID:SGTC_2137|Compound:5261020|FD-Score:4.92|P-value:1.19E-5 ID:SGTC_2276|Compound:7947513|FD-Score:4.62|P-value:3.76E-5 ID:SGTC_2341|Compound:7987486|FD-Score:-4.33|P-value:8.39E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-5.57|P-value:6.92E-7 ID:SGTC_2456|Compound:5770359|FD-Score:4.45|P-value:6.88E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.68|P-value:5.39E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:12.90|P-value:4.10E-29 ID:SGTC_2625|Compound:euphol|FD-Score:-5.29|P-value:2.23E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.62|P-value:2.86E-11 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.66|P-value:8.56E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.70|P-value:7.69E-4 ID:SGTC_3077|Compound:9117319|FD-Score:6.52|P-value:1.07E-8 ID:SGTC_3078|Compound:9116413|FD-Score:3.69|P-value:7.93E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:3.76|P-value:6.35E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:7.14|P-value:4.11E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:3.76|P-value:6.49E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:4.41|P-value:7.85E-5 ID:SGTC_255|Compound:tunicamycin|FD-Score:4.03|P-value:2.78E-4 ID:SGTC_263|Compound:alizarin|FD-Score:3.81|P-value:5.53E-4 ID:SGTC_467|Compound:ro 31-8220|FD-Score:-3.84|P-value:4.26E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.42|P-value:1.65E-6 ID:SGTC_525|Compound:k781-2675|FD-Score:4.78|P-value:2.09E-5 ID:SGTC_619|Compound:1652-1634|FD-Score:5.28|P-value:2.87E-6 ID:SGTC_776|Compound:4476-4378|FD-Score:3.68|P-value:8.22E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-7.37|P-value:8.87E-11 ID:SGTC_959|Compound:1222-0044|FD-Score:-3.95|P-value:3.00E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.68|P-value:8.08E-4 ID:SGTC_1174|Compound:0739-0097|FD-Score:4.02|P-value:2.87E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.60|P-value:3.93E-5 ID:SGTC_1275|Compound:0867-0008|FD-Score:-3.76|P-value:5.38E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.88|P-value:1.40E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:4.24|P-value:1.41E-4 ID:SGTC_1550|Compound:gossypolone|FD-Score:3.65|P-value:8.95E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:4.86|P-value:1.53E-5 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:9.88|P-value:9.31E-18 ID:SGTC_1658|Compound:st012944|FD-Score:3.72|P-value:7.25E-4 ID:SGTC_1687|Compound:st020778|FD-Score:4.14|P-value:1.94E-4 ID:SGTC_1693|Compound:xanthone|FD-Score:3.71|P-value:7.52E-4 ID:SGTC_1703|Compound:st026983|FD-Score:-4.16|P-value:1.53E-4 ID:SGTC_1750|Compound:st081859|FD-Score:5.87|P-value:2.35E-7 ID:SGTC_1844|Compound:rapamycin|FD-Score:11.50|P-value:1.59E-23 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:6.07|P-value:9.28E-8 ID:SGTC_2137|Compound:5261020|FD-Score:4.92|P-value:1.19E-5 ID:SGTC_2276|Compound:7947513|FD-Score:4.62|P-value:3.76E-5 ID:SGTC_2341|Compound:7987486|FD-Score:-4.33|P-value:8.39E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:-5.57|P-value:6.92E-7 ID:SGTC_2456|Compound:5770359|FD-Score:4.45|P-value:6.88E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.68|P-value:5.39E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:12.90|P-value:4.10E-29 ID:SGTC_2625|Compound:euphol|FD-Score:-5.29|P-value:2.23E-6 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.62|P-value:2.86E-11 ID:SGTC_2759|Compound:disulfiram|FD-Score:3.66|P-value:8.56E-4 ID:SGTC_3075|Compound:9116269|FD-Score:3.70|P-value:7.69E-4 ID:SGTC_3077|Compound:9117319|FD-Score:6.52|P-value:1.07E-8 ID:SGTC_3078|Compound:9116413|FD-Score:3.69|P-value:7.93E-4

Top fitness defect scores for YKR035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2619 obliquin 12.90 87.9 µM 4.10E-29 cell wall 11 1.41E-38
2 SGTC_1844 rapamycin 11.50 4.0 nM 1.59E-23 28 4.51E-31
3 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 9.88 54.9 µM 9.31E-18 redox potentiating 13 2.51E-23
4 SGTC_2679 rapamycin 7.62 1.0 nM 2.86E-11 46 1.27E-14
5 SGTC_145 3737-0113 7.14 38.4 µM 4.11E-10 DNA intercalators 14 4.52E-13
6 SGTC_3077 9117319 6.52 49.5 µM 1.07E-8 3 3.57E-11
7 SGTC_1926 methyl fluorone black 6.07 53.6 µM 9.28E-8 DNA intercalators 20 6.42E-10
8 SGTC_1750 st081859 5.87 2.4 µM 2.35E-7 endomembrane recycling 111 2.22E-9
9 SGTC_2582 agaric acid 5.68 100.0 µM 5.39E-7 redox potentiating 179 6.75E-9
10 SGTC_514 4466-0038 5.42 2.6 nM 1.65E-6 cell wall 93 3.01E-8
11 SGTC_619 1652-1634 5.28 108.0 µM 2.87E-6 PDR1 5 6.32E-8
12 SGTC_2137 5261020 4.92 140.0 µM 1.19E-5 22 4.22E-7
13 SGTC_1283 0922-0125 4.88 16.4 µM 1.40E-5 7 5.24E-7
14 SGTC_1583 clindamycin 4.86 39.6 µM 1.53E-5 mitochondrial processes 4 5.90E-7
15 SGTC_525 k781-2675 4.78 26.7 µM 2.09E-5 redox potentiating 35 8.94E-7
16 SGTC_2276 7947513 4.62 42.1 µM 3.76E-5 14 1.96E-6
17 SGTC_1269 0831-0796 4.60 3.0 µM 3.93E-5 60S ribosome export 6 2.07E-6
18 SGTC_2456 5770359 4.45 74.5 µM 6.88E-5 11 4.37E-6
19 SGTC_185 k048-0037 4.41 25.5 µM 7.85E-5 RSC & ERG11 5 5.21E-6
20 SGTC_1529 apigenin 4.24 74.0 µM 1.41E-4 29 1.14E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.316 1.58E-78 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.312 2.02E-76 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.245 7.73E-47 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.242 7.23E-46 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.233 1.80E-42 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.221 1.93E-38 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.221 2.67E-38 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.218 2.46E-37 YPL084W BRO1 hom Cytoplasmic class E vacuolar protein sorting (VPS) factor that coordinates deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
0.214 5.35E-36 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.213 1.11E-35 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.211 4.16E-35 YKL002W DID4 hom Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.209 1.58E-34 YPL002C SNF8 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.208 3.47E-34 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.207 7.20E-34 YMR077C VPS20 hom Myristoylated subunit of ESCRTIII, the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
0.206 2.05E-33 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans