YKR035W-A / DID2

Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR035W-A / DID2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.12|P-value:9.78E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:3.52|P-value:2.33E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:9.18|P-value:3.59E-20 ID:SGTC_343|Compound:4130-2827|FD-Score:4.34|P-value:8.09E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:6.76|P-value:9.06E-12 ID:SGTC_350|Compound:k064-0020|FD-Score:3.14|P-value:9.05E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.53|P-value:2.20E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.36|P-value:4.29E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.28|P-value:7.41E-8 ID:SGTC_627|Compound:1391-0720|FD-Score:3.14|P-value:9.02E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:4.08|P-value:2.44E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.57|P-value:1.90E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.75|P-value:9.49E-5 ID:SGTC_931|Compound:2812-0076|FD-Score:3.34|P-value:4.45E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.64|P-value:1.47E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.45|P-value:3.01E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.49|P-value:2.66E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.81|P-value:7.48E-5 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.24|P-value:1.24E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.59|P-value:2.46E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:6.03|P-value:1.04E-9 ID:SGTC_1658|Compound:st012944|FD-Score:4.51|P-value:3.72E-6 ID:SGTC_1750|Compound:st081859|FD-Score:6.61|P-value:2.41E-11 ID:SGTC_1766|Compound:st045414|FD-Score:3.48|P-value:2.70E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.40|P-value:3.31E-17 ID:SGTC_1962|Compound:st076595|FD-Score:4.16|P-value:1.73E-5 ID:SGTC_2077|Compound:5212015|FD-Score:3.64|P-value:1.46E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.02|P-value:3.20E-5 ID:SGTC_2336|Compound:9001819|FD-Score:4.17|P-value:1.68E-5 ID:SGTC_2449|Compound:5812982|FD-Score:4.19|P-value:1.51E-5 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:4.42|P-value:5.52E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.37|P-value:4.05E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.16|P-value:1.44E-7 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.39|P-value:1.01E-13 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.48|P-value:2.68E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.63|P-value:1.11E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.08|P-value:2.52E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.67|P-value:1.33E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.83|P-value:7.14E-5 ID:SGTC_2817|Compound:7995342|FD-Score:5.68|P-value:7.93E-9 ID:SGTC_2862|Compound:9027634|FD-Score:3.46|P-value:2.84E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.23|P-value:6.79E-4 ID:SGTC_3020|Compound:9089140|FD-Score:4.33|P-value:8.14E-6 ID:SGTC_3159|Compound:9100096|FD-Score:5.46|P-value:2.88E-8 ID:SGTC_3214|Compound:9129694|FD-Score:3.62|P-value:1.59E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.16|P-value:8.34E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.12|P-value:9.78E-4 ID:SGTC_202|Compound:3594-0513|FD-Score:3.52|P-value:2.33E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:9.18|P-value:3.59E-20 ID:SGTC_343|Compound:4130-2827|FD-Score:4.34|P-value:8.09E-6 ID:SGTC_344|Compound:k064-0035|FD-Score:6.76|P-value:9.06E-12 ID:SGTC_350|Compound:k064-0020|FD-Score:3.14|P-value:9.05E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:3.53|P-value:2.20E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.36|P-value:4.29E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.28|P-value:7.41E-8 ID:SGTC_627|Compound:1391-0720|FD-Score:3.14|P-value:9.02E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:4.08|P-value:2.44E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.57|P-value:1.90E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:3.75|P-value:9.49E-5 ID:SGTC_931|Compound:2812-0076|FD-Score:3.34|P-value:4.45E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:3.64|P-value:1.47E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:3.45|P-value:3.01E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.49|P-value:2.66E-4 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:3.81|P-value:7.48E-5 ID:SGTC_1475|Compound:4092-1048|FD-Score:4.24|P-value:1.24E-5 ID:SGTC_1495|Compound:4469-0029|FD-Score:4.59|P-value:2.46E-6 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:6.03|P-value:1.04E-9 ID:SGTC_1658|Compound:st012944|FD-Score:4.51|P-value:3.72E-6 ID:SGTC_1750|Compound:st081859|FD-Score:6.61|P-value:2.41E-11 ID:SGTC_1766|Compound:st045414|FD-Score:3.48|P-value:2.70E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:8.40|P-value:3.31E-17 ID:SGTC_1962|Compound:st076595|FD-Score:4.16|P-value:1.73E-5 ID:SGTC_2077|Compound:5212015|FD-Score:3.64|P-value:1.46E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.02|P-value:3.20E-5 ID:SGTC_2336|Compound:9001819|FD-Score:4.17|P-value:1.68E-5 ID:SGTC_2449|Compound:5812982|FD-Score:4.19|P-value:1.51E-5 ID:SGTC_2558|Compound:carylophyllene oxide|FD-Score:4.42|P-value:5.52E-6 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.37|P-value:4.05E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.16|P-value:1.44E-7 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.39|P-value:1.01E-13 ID:SGTC_2688|Compound:kanamycin b|FD-Score:3.48|P-value:2.68E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:-5.63|P-value:1.11E-8 ID:SGTC_2756|Compound:carbadox|FD-Score:-4.08|P-value:2.52E-5 ID:SGTC_2760|Compound:etidronate|FD-Score:-3.67|P-value:1.33E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-3.83|P-value:7.14E-5 ID:SGTC_2817|Compound:7995342|FD-Score:5.68|P-value:7.93E-9 ID:SGTC_2862|Compound:9027634|FD-Score:3.46|P-value:2.84E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.23|P-value:6.79E-4 ID:SGTC_3020|Compound:9089140|FD-Score:4.33|P-value:8.14E-6 ID:SGTC_3159|Compound:9100096|FD-Score:5.46|P-value:2.88E-8 ID:SGTC_3214|Compound:9129694|FD-Score:3.62|P-value:1.59E-4 ID:SGTC_3281|Compound:9140613|FD-Score:3.16|P-value:8.34E-4

Top fitness defect scores for YKR035W-A deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_255 tunicamycin 9.18 200.5 nM 3.59E-20 unfolded protein response 15 2.18E-20
2 SGTC_1844 rapamycin 8.40 4.0 nM 3.31E-17 50 2.18E-17
3 SGTC_2679 rapamycin 7.39 1.0 nM 1.01E-13 47 7.33E-14
4 SGTC_344 k064-0035 6.76 6.4 µM 9.06E-12 60S ribosome export 2 6.96E-12
5 SGTC_1750 st081859 6.61 2.4 µM 2.41E-11 endomembrane recycling 94 1.87E-11
6 SGTC_1593 justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one 6.03 54.9 µM 1.04E-9 redox potentiating 32 8.43E-10
7 SGTC_2817 7995342 5.68 71.4 µM 7.93E-9 5 6.59E-9
8 SGTC_3159 9100096 5.46 49.5 µM 2.88E-8 5 2.43E-8
9 SGTC_512 prima-1 5.28 270.0 µM 7.41E-8 DNA intercalators 8 6.32E-8
10 SGTC_2582 agaric acid 5.16 100.0 µM 1.44E-7 redox potentiating 213 1.24E-7
11 SGTC_1495 4469-0029 4.59 237.0 µM 2.46E-6 82 2.18E-6
12 SGTC_1658 st012944 4.51 20.0 µM 3.72E-6 iron homeostasis 61 3.32E-6
13 SGTC_2558 carylophyllene oxide 4.42 100.0 µM 5.52E-6 6 4.95E-6
14 SGTC_343 4130-2827 4.34 29.8 µM 8.09E-6 23 7.28E-6
15 SGTC_3020 9089140 4.33 49.5 µM 8.14E-6 17 7.33E-6
16 SGTC_1475 4092-1048 4.24 11.7 µM 1.24E-5 plasma membrane duress 26 1.12E-5
17 SGTC_2449 5812982 4.19 45.8 µM 1.51E-5 7 1.37E-5
18 SGTC_2336 9001819 4.17 198.7 µM 1.68E-5 heme biosynthesis & mitochondrial translocase 45 1.53E-5
19 SGTC_1962 st076595 4.16 64.5 µM 1.73E-5 redox potentiating 21 1.57E-5
20 SGTC_786 4544-0069 4.08 423.0 µM 2.44E-5 20 2.22E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.277 3.24E-60 YNR005C_d YNR005C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.262 1.28E-53 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.253 2.85E-50 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.250 3.82E-49 YPR173C VPS4 hom AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
0.245 7.73E-47 YKR035C_d OPI8_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
0.235 1.89E-43 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.235 3.20E-43 YNR006W VPS27 hom Endosomal protein that forms a complex with Hse1p; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation; has Ubiquitin Interaction Motifs which bind ubiquitin (Ubi4p)
0.235 2.77E-43 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.218 2.18E-37 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.207 8.06E-34 YKL002W DID4 hom Class E Vps protein of the ESCRT-III complex, required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
0.207 9.18E-34 YPL101W ELP4 hom Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
0.203 1.46E-32 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.202 4.62E-32 YNL119W NCS2 hom Protein required for thiolation of the uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.200 1.39E-31 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.197 8.89E-31 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin