YKR036C / CAF4

WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR036C / CAF4

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:-3.30|P-value:5.10E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.10|P-value:7.83E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.41|P-value:2.58E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.76|P-value:6.54E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:3.29|P-value:3.91E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.15|P-value:8.60E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.27|P-value:4.19E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.14|P-value:6.86E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.04|P-value:1.98E-5 ID:SGTC_754|Compound:3474-0146|FD-Score:-3.57|P-value:1.86E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.54|P-value:1.53E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.10|P-value:7.74E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.53|P-value:1.59E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.96|P-value:2.78E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.51|P-value:2.33E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.55|P-value:1.49E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.68|P-value:8.81E-5 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.30|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.30|P-value:3.79E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.31|P-value:5.83E-6 ID:SGTC_1684|Compound:st024538|FD-Score:-3.11|P-value:9.72E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.76|P-value:6.53E-5 ID:SGTC_1795|Compound:st049545|FD-Score:3.31|P-value:3.68E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.06|P-value:8.98E-4 ID:SGTC_1994|Compound:st072947|FD-Score:5.13|P-value:9.24E-8 ID:SGTC_2049|Compound:5262831|FD-Score:-3.17|P-value:7.97E-4 ID:SGTC_2060|Compound:5238662|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_2282|Compound:7944268|FD-Score:5.53|P-value:9.97E-9 ID:SGTC_2291|Compound:7917025|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.55|P-value:1.50E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.84|P-value:4.57E-5 ID:SGTC_2819|Compound:7996655|FD-Score:4.65|P-value:1.12E-6 ID:SGTC_2826|Compound:7992541|FD-Score:-3.73|P-value:9.82E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.19|P-value:1.01E-5 ID:SGTC_2835|Compound:9006588|FD-Score:5.63|P-value:5.44E-9 ID:SGTC_2905|Compound:7799065|FD-Score:3.31|P-value:3.63E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.17|P-value:7.91E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.68|P-value:1.21E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.13|P-value:1.34E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.35|P-value:4.19E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_3043|Compound:9092303|FD-Score:-4.38|P-value:5.89E-6 ID:SGTC_3185|Compound:9108267|FD-Score:-3.43|P-value:3.09E-4 ID:SGTC_3193|Compound:9111931|FD-Score:4.02|P-value:2.12E-5 ID:SGTC_3279|Compound:9140807|FD-Score:3.33|P-value:3.37E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.32|P-value:3.49E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:-3.30|P-value:5.10E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.10|P-value:7.83E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:3.41|P-value:2.58E-4 ID:SGTC_346|Compound:4513-0042|FD-Score:3.76|P-value:6.54E-5 ID:SGTC_361|Compound:000s-0510|FD-Score:3.29|P-value:3.91E-4 ID:SGTC_523|Compound:1934-0222|FD-Score:-3.15|P-value:8.60E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.27|P-value:4.19E-4 ID:SGTC_632|Compound:0781-2353|FD-Score:3.14|P-value:6.86E-4 ID:SGTC_672|Compound:1441-0018|FD-Score:4.04|P-value:1.98E-5 ID:SGTC_754|Compound:3474-0146|FD-Score:-3.57|P-value:1.86E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:-3.17|P-value:7.96E-4 ID:SGTC_769|Compound:0302-0231|FD-Score:3.54|P-value:1.53E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.10|P-value:7.74E-4 ID:SGTC_865|Compound:0488-0499|FD-Score:3.53|P-value:1.59E-4 ID:SGTC_952|Compound:1185-0122|FD-Score:3.96|P-value:2.78E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:-3.51|P-value:2.33E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.55|P-value:1.49E-4 ID:SGTC_1198|Compound:1612-1005|FD-Score:3.68|P-value:8.81E-5 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.30|P-value:3.76E-4 ID:SGTC_1526|Compound:st051518|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:3.30|P-value:3.79E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:4.31|P-value:5.83E-6 ID:SGTC_1684|Compound:st024538|FD-Score:-3.11|P-value:9.72E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.76|P-value:6.53E-5 ID:SGTC_1795|Compound:st049545|FD-Score:3.31|P-value:3.68E-4 ID:SGTC_1930|Compound:st074634|FD-Score:3.06|P-value:8.98E-4 ID:SGTC_1994|Compound:st072947|FD-Score:5.13|P-value:9.24E-8 ID:SGTC_2049|Compound:5262831|FD-Score:-3.17|P-value:7.97E-4 ID:SGTC_2060|Compound:5238662|FD-Score:-3.44|P-value:2.98E-4 ID:SGTC_2282|Compound:7944268|FD-Score:5.53|P-value:9.97E-9 ID:SGTC_2291|Compound:7917025|FD-Score:-3.71|P-value:1.05E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.55|P-value:1.50E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.84|P-value:4.57E-5 ID:SGTC_2819|Compound:7996655|FD-Score:4.65|P-value:1.12E-6 ID:SGTC_2826|Compound:7992541|FD-Score:-3.73|P-value:9.82E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.19|P-value:1.01E-5 ID:SGTC_2835|Compound:9006588|FD-Score:5.63|P-value:5.44E-9 ID:SGTC_2905|Compound:7799065|FD-Score:3.31|P-value:3.63E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.17|P-value:7.91E-4 ID:SGTC_3020|Compound:9089140|FD-Score:-3.68|P-value:1.21E-4 ID:SGTC_3027|Compound:9089541|FD-Score:4.13|P-value:1.34E-5 ID:SGTC_3028|Compound:9087778|FD-Score:-3.35|P-value:4.19E-4 ID:SGTC_3032|Compound:9089427|FD-Score:-3.50|P-value:2.39E-4 ID:SGTC_3039|Compound:9090270|FD-Score:3.63|P-value:1.09E-4 ID:SGTC_3043|Compound:9092303|FD-Score:-4.38|P-value:5.89E-6 ID:SGTC_3185|Compound:9108267|FD-Score:-3.43|P-value:3.09E-4 ID:SGTC_3193|Compound:9111931|FD-Score:4.02|P-value:2.12E-5 ID:SGTC_3279|Compound:9140807|FD-Score:3.33|P-value:3.37E-4 ID:SGTC_3282|Compound:9108498|FD-Score:3.32|P-value:3.49E-4

Top fitness defect scores for YKR036C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2835 9006588 5.63 71.4 µM 5.44E-9 10 8.96E-9
2 SGTC_2282 7944268 5.53 200.0 µM 9.97E-9 RPP1 & pyrimidine depletion 8 1.62E-8
3 SGTC_1994 st072947 5.13 40.9 µM 9.24E-8 4 1.43E-7
4 SGTC_2819 7996655 4.65 46.4 µM 1.12E-6 9 1.63E-6
5 SGTC_1681 tripelennamine 4.31 100.0 µM 5.83E-6 14 8.18E-6
6 SGTC_2833 9001315 4.19 45.5 µM 1.01E-5 amide catabolism 48 1.40E-5
7 SGTC_3027 9089541 4.13 49.5 µM 1.34E-5 fatty acid desaturase (OLE1) 20 1.84E-5
8 SGTC_672 1441-0018 4.04 53.7 µM 1.98E-5 18 2.70E-5
9 SGTC_3193 9111931 4.02 49.5 µM 2.12E-5 22 2.89E-5
10 SGTC_952 1185-0122 3.96 232.0 µM 2.78E-5 20 3.75E-5
11 SGTC_2713 st079149 3.84 53.4 µM 4.57E-5 24 6.10E-5
12 SGTC_1768 st044301 3.76 37.2 µM 6.53E-5 21 8.64E-5
13 SGTC_346 4513-0042 3.76 7.2 µM 6.54E-5 azole & statin 48 8.66E-5
14 SGTC_1198 1612-1005 3.68 86.7 µM 8.81E-5 19 1.16E-4
15 SGTC_3039 9090270 3.63 49.5 µM 1.09E-4 RPP1 & pyrimidine depletion 17 1.43E-4
16 SGTC_1091 telmisartan 3.55 47.6 µM 1.49E-4 28 1.93E-4
17 SGTC_2597 laccaic acid a 3.55 100.0 µM 1.50E-4 45 1.94E-4
18 SGTC_769 0302-0231 3.54 76.0 µM 1.53E-4 62 1.99E-4
19 SGTC_865 0488-0499 3.53 301.0 µM 1.59E-4 25 2.06E-4
20 SGTC_312 4092-0653 3.41 6.8 µM 2.58E-4 ergosterol depletion effects on membrane 112 3.30E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.76E-9 YDR056C_p YDR056C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR056C is not an essential protein
0.090 2.02E-7 YDL212W SHR3 het Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
0.082 2.01E-6 YMR172W HOT1 hom Transcription factor required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
0.082 2.11E-6 YFR040W SAP155 hom Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress
0.081 2.54E-6 YDR246W-A_p YDR246W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.079 4.82E-6 YLR023C IZH3 hom Membrane protein involved in zinc ion homeostasis, member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
0.076 1.10E-5 YHR122W CIA2 het Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Met18p that directs Fe-S cluster incorporation into a subset of proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human FAM96B
0.074 1.83E-5 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.074 1.96E-5 YGL122C NAB2 het Nuclear polyadenylated RNA-binding protein; required for nuclear mRNA export and poly(A) tail length control; binds nuclear pore protein Mlp1p; autoregulates mRNA levels; related to human hnRNPs; nuclear localization sequence binds Kap104p; protein abundance increases in response to DNA replication stress
0.074 1.97E-5 YOR057W SGT1 het Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
0.073 2.03E-5 YNL272C SEC2 het Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
0.072 2.73E-5 YFL055W AGP3 hom Low-affinity amino acid permease, may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
0.072 3.00E-5 YER009W NTF2 het Nuclear envelope protein, interacts with GDP-bound Gsp1p and with proteins of the nuclear pore to transport Gsp1p into the nucleus where it is an essential player in nucleocytoplasmic transport
0.072 3.25E-5 YIR025W MND2 hom Subunit of the anaphase-promoting complex (APC); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
0.071 3.81E-5 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy