YKR037C / SPC34

Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR037C / SPC34

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:-3.54|P-value:3.26E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.87|P-value:9.41E-5 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.91|P-value:8.19E-5 ID:SGTC_560|Compound:2332-1943|FD-Score:4.24|P-value:1.44E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.59|P-value:2.66E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.45|P-value:3.21E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.19|P-value:7.83E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.51|P-value:2.57E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.22|P-value:7.08E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-4.32|P-value:1.48E-5 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.54|P-value:3.17E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-3.40|P-value:5.19E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.19|P-value:7.96E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.64|P-value:2.32E-6 ID:SGTC_1650|Compound:st011916|FD-Score:-3.24|P-value:9.12E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.33|P-value:6.78E-4 ID:SGTC_1676|Compound:st018489|FD-Score:4.66|P-value:2.15E-6 ID:SGTC_1714|Compound:st033236|FD-Score:-3.69|P-value:1.84E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-4.10|P-value:3.71E-5 ID:SGTC_1999|Compound:st074831|FD-Score:-4.67|P-value:3.21E-6 ID:SGTC_2021|Compound:4019055|FD-Score:-3.31|P-value:7.23E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-4.52|P-value:6.37E-6 ID:SGTC_2072|Compound:5231554|FD-Score:3.12|P-value:9.91E-4 ID:SGTC_2093|Compound:5331342|FD-Score:-5.65|P-value:2.26E-8 ID:SGTC_2330|Compound:7979373|FD-Score:4.33|P-value:9.58E-6 ID:SGTC_2367|Compound:9070478|FD-Score:4.27|P-value:1.25E-5 ID:SGTC_2383|Compound:9036125|FD-Score:4.44|P-value:5.95E-6 ID:SGTC_2429|Compound:5902844|FD-Score:3.25|P-value:6.50E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.33|P-value:6.81E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-7.20|P-value:1.46E-12 ID:SGTC_2947|Compound:9072639|FD-Score:-3.62|P-value:2.43E-4 ID:SGTC_3013|Compound:9080133|FD-Score:-3.29|P-value:7.79E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.18|P-value:8.09E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.72|P-value:1.17E-4 ID:SGTC_46|Compound:0329-0187a|FD-Score:-3.54|P-value:3.26E-4 ID:SGTC_75|Compound:0159-0059|FD-Score:-3.87|P-value:9.41E-5 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.91|P-value:8.19E-5 ID:SGTC_560|Compound:2332-1943|FD-Score:4.24|P-value:1.44E-5 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.59|P-value:2.66E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:3.45|P-value:3.21E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.19|P-value:7.83E-4 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.51|P-value:2.57E-4 ID:SGTC_1193|Compound:1488-0126|FD-Score:3.22|P-value:7.08E-4 ID:SGTC_1208|Compound:2323-2347|FD-Score:-4.32|P-value:1.48E-5 ID:SGTC_1211|Compound:1056-0033|FD-Score:-3.54|P-value:3.17E-4 ID:SGTC_1215|Compound:3852-0577|FD-Score:-3.40|P-value:5.19E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.19|P-value:7.96E-4 ID:SGTC_1354|Compound:1498-1681|FD-Score:4.64|P-value:2.32E-6 ID:SGTC_1650|Compound:st011916|FD-Score:-3.24|P-value:9.12E-4 ID:SGTC_1652|Compound:st011932|FD-Score:-3.33|P-value:6.78E-4 ID:SGTC_1676|Compound:st018489|FD-Score:4.66|P-value:2.15E-6 ID:SGTC_1714|Compound:st033236|FD-Score:-3.69|P-value:1.84E-4 ID:SGTC_1927|Compound:st060202|FD-Score:-4.10|P-value:3.71E-5 ID:SGTC_1999|Compound:st074831|FD-Score:-4.67|P-value:3.21E-6 ID:SGTC_2021|Compound:4019055|FD-Score:-3.31|P-value:7.23E-4 ID:SGTC_2022|Compound:5136654|FD-Score:-4.52|P-value:6.37E-6 ID:SGTC_2072|Compound:5231554|FD-Score:3.12|P-value:9.91E-4 ID:SGTC_2093|Compound:5331342|FD-Score:-5.65|P-value:2.26E-8 ID:SGTC_2330|Compound:7979373|FD-Score:4.33|P-value:9.58E-6 ID:SGTC_2367|Compound:9070478|FD-Score:4.27|P-value:1.25E-5 ID:SGTC_2383|Compound:9036125|FD-Score:4.44|P-value:5.95E-6 ID:SGTC_2429|Compound:5902844|FD-Score:3.25|P-value:6.50E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.74|P-value:1.08E-4 ID:SGTC_2772|Compound:vinpocetine|FD-Score:-3.33|P-value:6.81E-4 ID:SGTC_2849|Compound:9019321|FD-Score:-7.20|P-value:1.46E-12 ID:SGTC_2947|Compound:9072639|FD-Score:-3.62|P-value:2.43E-4 ID:SGTC_3013|Compound:9080133|FD-Score:-3.29|P-value:7.79E-4 ID:SGTC_3058|Compound:9093861|FD-Score:3.18|P-value:8.09E-4 ID:SGTC_3112|Compound:9123419|FD-Score:3.72|P-value:1.17E-4

Top fitness defect scores for YKR037C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1676 st018489 4.66 23.4 µM 2.15E-6 60S ribosome export 4 1.57E-6 0.91
2 SGTC_1354 1498-1681 4.64 81.0 µM 2.32E-6 2 1.70E-6 0.03
3 SGTC_2383 9036125 4.44 200.0 µM 5.95E-6 1 4.51E-6 0.63
4 SGTC_2330 7979373 4.33 200.0 µM 9.58E-6 60S ribosome export 6 7.40E-6 0.09
5 SGTC_2367 9070478 4.27 135.4 µM 1.25E-5 cell wall 8 9.73E-6 0.11
6 SGTC_560 2332-1943 4.24 155.0 µM 1.44E-5 2 1.13E-5 0.34
7 SGTC_2639 lactacystin 3.74 53.0 µM 1.08E-4 11 9.13E-5 0.19
8 SGTC_3112 9123419 3.72 49.5 µM 1.17E-4 RPP1 & pyrimidine depletion 7 9.85E-5 0.10
9 SGTC_1093 fluphenazine 3.51 8.0 µM 2.57E-4 8 2.23E-4 0.05
10 SGTC_666 0272-0154 3.45 136.0 µM 3.21E-4 cell wall 22 2.80E-4 0.02
11 SGTC_2429 5902844 3.25 64.4 µM 6.50E-4 13 5.82E-4 0.15
12 SGTC_1193 1488-0126 3.22 41.5 µM 7.08E-4 9 6.35E-4 0.06
13 SGTC_695 3505-6186 3.19 243.0 µM 7.83E-4 23 7.05E-4 0.03
14 SGTC_1285 0929-0069 3.19 65.5 µM 7.96E-4 9 7.18E-4 0.13
15 SGTC_3058 9093861 3.18 49.5 µM 8.09E-4 9 7.29E-4 0.04
16 SGTC_2072 5231554 3.12 129.0 µM 9.91E-4 9 8.99E-4 0.00
17 SGTC_2090 5301565 3.09 104.5 µM 0.00110 TSC3-RPN4 18 0.00100 0.07
18 SGTC_1839 st053978 3.00 48.0 µM 0.00145 6 0.00134 0.22
19 SGTC_3094 9117742 3.00 49.5 µM 0.00146 15 0.00134 0.11
20 SGTC_2198 6589944 3.00 200.0 µM 0.00146 9 0.00135 0.00

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.102 3.19E-9 YMR059W SEN15 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
0.094 4.56E-8 YBR025C OLA1 hom P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
0.080 3.51E-6 YLR183C TOS4 hom Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; expression is periodic and peaks in G1; relative distribution to the nucleus increases upon DNA replication stress; TOS4 has a paralog, PLM2, that arose from the whole genome duplication
0.078 7.04E-6 YMR278W PGM3 hom Phosphoglucomutase, catalyzes interconversion of glucose-1-phosphate and glucose-6-phospate; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
0.076 1.01E-5 YKL154W SRP102 het Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
0.071 3.65E-5 YER073W ALD5 hom Mitochondrial aldehyde dehydrogenase, involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
0.070 4.76E-5 YDR368W YPR1 hom NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication
0.069 6.25E-5 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.068 9.10E-5 YGR112W SHY1 hom Mitochondrial inner membrane protein required for assembly of cytochrome c oxidase (complex IV); associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome
0.067 9.50E-5 YMR028W TAP42 het Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
0.067 1.09E-4 YGR172C YIP1 het Integral membrane protein required for the biogenesis of ER-derived COPII transport vesicles; interacts with Yif1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF4
0.065 1.51E-4 YEL017W GTT3 hom Protein of unknown function with a possible role in glutathione metabolism, as suggested by computational analysis of large-scale protein-protein interaction data; GFP-fusion protein localizes to the nuclear periphery
0.065 1.68E-4 YER105C NUP157 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
0.063 2.87E-4 YJR021C REC107 hom Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
0.062 3.37E-4 YJL064W_d YJL064W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1