YKR038C / KAE1

Highly conserved ATPase of HSP70/DnaK family; component of the EKC/KEOPS complex with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR038C / KAE1

Click on Significant Values for Screen Details ID:SGTC_159|Compound:3970-0959|FD-Score:4.09|P-value:2.36E-6 ID:SGTC_394|Compound:menadione|FD-Score:3.36|P-value:8.41E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.36|P-value:8.39E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:2.83|P-value:7.76E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.83|P-value:9.40E-6 ID:SGTC_648|Compound:0375-0473|FD-Score:3.33|P-value:9.78E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.36|P-value:8.69E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:2.94|P-value:5.00E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.62|P-value:2.58E-5 ID:SGTC_917|Compound:harmaline|FD-Score:-3.21|P-value:1.80E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.90|P-value:6.40E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:2.93|P-value:5.17E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.84|P-value:8.93E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.25|P-value:1.53E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.20|P-value:1.88E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.19|P-value:1.76E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.32|P-value:1.03E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.15|P-value:2.15E-4 ID:SGTC_1637|Compound:st007924|FD-Score:2.87|P-value:6.70E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.25|P-value:1.36E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.82|P-value:9.46E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-4.23|P-value:1.23E-6 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.55|P-value:3.58E-5 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.78|P-value:1.20E-5 ID:SGTC_2156|Compound:5649887|FD-Score:4.04|P-value:3.19E-6 ID:SGTC_2164|Compound:5685496|FD-Score:3.64|P-value:2.35E-5 ID:SGTC_2194|Compound:5927483|FD-Score:-2.87|P-value:7.13E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.42|P-value:6.55E-5 ID:SGTC_2302|Compound:7771753|FD-Score:2.97|P-value:4.45E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.21|P-value:1.62E-4 ID:SGTC_2505|Compound:galangin|FD-Score:2.76|P-value:9.92E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_2515|Compound:chrysin|FD-Score:3.44|P-value:6.06E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.53|P-value:4.22E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.99|P-value:4.09E-6 ID:SGTC_2784|Compound:7567673|FD-Score:-2.89|P-value:6.57E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.94|P-value:5.39E-4 ID:SGTC_2814|Compound:7977482|FD-Score:2.90|P-value:5.83E-4 ID:SGTC_2900|Compound:5328528|FD-Score:4.33|P-value:6.49E-7 ID:SGTC_2926|Compound:9010726|FD-Score:5.71|P-value:8.62E-11 ID:SGTC_3210|Compound:9128157|FD-Score:3.02|P-value:3.57E-4 ID:SGTC_3232|Compound:9132874|FD-Score:2.84|P-value:7.28E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:4.09|P-value:2.36E-6 ID:SGTC_394|Compound:menadione|FD-Score:3.36|P-value:8.41E-5 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.36|P-value:8.39E-5 ID:SGTC_524|Compound:r073-0010|FD-Score:2.83|P-value:7.76E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.83|P-value:9.40E-6 ID:SGTC_648|Compound:0375-0473|FD-Score:3.33|P-value:9.78E-5 ID:SGTC_686|Compound:1319-0111|FD-Score:3.36|P-value:8.69E-5 ID:SGTC_763|Compound:3453-1109|FD-Score:2.94|P-value:5.00E-4 ID:SGTC_780|Compound:0109-0199|FD-Score:3.62|P-value:2.58E-5 ID:SGTC_917|Compound:harmaline|FD-Score:-3.21|P-value:1.80E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.90|P-value:6.40E-6 ID:SGTC_1048|Compound:3161-0300|FD-Score:2.93|P-value:5.17E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:3.84|P-value:8.93E-6 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.25|P-value:1.53E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.20|P-value:1.88E-4 ID:SGTC_1127|Compound:asiatic acid|FD-Score:3.19|P-value:1.76E-4 ID:SGTC_1381|Compound:2324-0066|FD-Score:3.32|P-value:1.03E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:3.15|P-value:2.15E-4 ID:SGTC_1637|Compound:st007924|FD-Score:2.87|P-value:6.70E-4 ID:SGTC_1653|Compound:st012112|FD-Score:3.25|P-value:1.36E-4 ID:SGTC_1705|Compound:st027868|FD-Score:3.82|P-value:9.46E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-4.23|P-value:1.23E-6 ID:SGTC_1788|Compound:fluconazole|FD-Score:3.55|P-value:3.58E-5 ID:SGTC_1878|Compound:temozolomide|FD-Score:3.78|P-value:1.20E-5 ID:SGTC_2156|Compound:5649887|FD-Score:4.04|P-value:3.19E-6 ID:SGTC_2164|Compound:5685496|FD-Score:3.64|P-value:2.35E-5 ID:SGTC_2194|Compound:5927483|FD-Score:-2.87|P-value:7.13E-4 ID:SGTC_2196|Compound:6573537|FD-Score:3.42|P-value:6.55E-5 ID:SGTC_2302|Compound:7771753|FD-Score:2.97|P-value:4.45E-4 ID:SGTC_2382|Compound:9035899|FD-Score:3.21|P-value:1.62E-4 ID:SGTC_2505|Compound:galangin|FD-Score:2.76|P-value:9.92E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:3.66|P-value:2.15E-5 ID:SGTC_2515|Compound:chrysin|FD-Score:3.44|P-value:6.06E-5 ID:SGTC_2587|Compound:piperic acid|FD-Score:-3.53|P-value:4.22E-5 ID:SGTC_2764|Compound:methyldopa|FD-Score:3.99|P-value:4.09E-6 ID:SGTC_2784|Compound:7567673|FD-Score:-2.89|P-value:6.57E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.94|P-value:5.39E-4 ID:SGTC_2814|Compound:7977482|FD-Score:2.90|P-value:5.83E-4 ID:SGTC_2900|Compound:5328528|FD-Score:4.33|P-value:6.49E-7 ID:SGTC_2926|Compound:9010726|FD-Score:5.71|P-value:8.62E-11 ID:SGTC_3210|Compound:9128157|FD-Score:3.02|P-value:3.57E-4 ID:SGTC_3232|Compound:9132874|FD-Score:2.84|P-value:7.28E-4

Top fitness defect scores for YKR038C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2926 9010726 5.71 65.2 µM 8.62E-11 1 5.53E-9 1.22
2 SGTC_2900 5328528 4.33 85.3 µM 6.49E-7 Golgi 3 7.47E-6 0.12
3 SGTC_159 3970-0959 4.09 50.0 µM 2.36E-6 16 2.13E-5 0.08
4 SGTC_2156 5649887 4.04 115.0 µM 3.19E-6 RPP1 & pyrimidine depletion 16 2.71E-5 0.02
5 SGTC_2764 methyldopa 3.99 94.7 µM 4.09E-6 4 3.32E-5 0.34
6 SGTC_1001 4048-4355 3.90 44.4 µM 6.40E-6 copper-dependent oxidative stress 10 4.76E-5 0.12
7 SGTC_1078 idebenone 3.84 36.1 µM 8.93E-6 Golgi 6 6.24E-5 0.32
8 SGTC_537 1334-0398 3.83 209.0 µM 9.40E-6 6 6.50E-5 0.00
9 SGTC_1705 st027868 3.82 56.3 µM 9.46E-6 2 6.54E-5 0.29
10 SGTC_1878 temozolomide 3.78 1.0 mM 1.20E-5 3 7.93E-5 0.29
11 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 3.66 54.6 µM 2.15E-5 RPP1 & pyrimidine depletion 14 1.27E-4 0.01
12 SGTC_2164 5685496 3.64 200.0 µM 2.35E-5 9 1.37E-4 0.02
13 SGTC_780 0109-0199 3.62 192.0 µM 2.58E-5 6 1.47E-4 0.20
14 SGTC_1788 fluconazole 3.55 20.0 µM 3.58E-5 azole & statin 12 1.92E-4 0.12
15 SGTC_2515 chrysin 3.44 20.8 µM 6.06E-5 RPP1 & pyrimidine depletion 8 2.95E-4 0.17
16 SGTC_2196 6573537 3.42 42.7 µM 6.55E-5 copper-dependent oxidative stress 6 3.14E-4 0.02
17 SGTC_445 e6 berbamine 3.36 66.0 µM 8.39E-5 NEO1-PIK1 38 3.84E-4 0.04
18 SGTC_394 menadione 3.36 8.3 µM 8.41E-5 superoxide 12 3.85E-4 0.16
19 SGTC_686 1319-0111 3.36 510.0 µM 8.69E-5 60S ribosome export 13 3.95E-4 0.07
20 SGTC_648 0375-0473 3.33 33.3 µM 9.78E-5 10 4.35E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 7.47E-23 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.145 3.50E-17 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.119 3.84E-12 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.119 3.86E-12 YKL001C MET14 hom Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
0.116 1.84E-11 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.109 3.04E-10 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.102 3.72E-9 YDR094W_d YDR094W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2
0.101 4.47E-9 YOR209C NPT1 hom Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
0.096 2.81E-8 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.094 4.49E-8 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.094 5.00E-8 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.093 7.08E-8 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.092 8.43E-8 YDR171W HSP42 hom Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
0.090 1.87E-7 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.089 2.87E-7 YLR370C ARC18 hom Subunit of the ARP2/3 complex, which is required for the motility and integrity of cortical actin patches