YKR043C / SHB17

Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR043C / SHB17

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:6.08|P-value:3.13E-8 ID:SGTC_78|Compound:0336-0123|FD-Score:5.17|P-value:2.17E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:3.73|P-value:4.88E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.71|P-value:5.23E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:4.70|P-value:1.52E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:4.16|P-value:1.14E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.28|P-value:7.61E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.80|P-value:2.52E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:5.66|P-value:2.46E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.91|P-value:2.69E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.02|P-value:1.90E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:6.94|P-value:3.18E-10 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.19|P-value:1.05E-4 ID:SGTC_1937|Compound:st074714|FD-Score:4.99|P-value:4.69E-6 ID:SGTC_2015|Compound:5119600|FD-Score:-7.69|P-value:1.56E-12 ID:SGTC_2024|Compound:5144227|FD-Score:-4.53|P-value:1.78E-5 ID:SGTC_2110|Compound:5483698|FD-Score:3.67|P-value:6.00E-4 ID:SGTC_2117|Compound:5521686|FD-Score:3.59|P-value:7.65E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.74|P-value:3.14E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.09|P-value:9.23E-5 ID:SGTC_2366|Compound:9070441|FD-Score:-3.73|P-value:3.27E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.22|P-value:9.33E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:6.13|P-value:2.51E-8 ID:SGTC_2842|Compound:9000675|FD-Score:4.41|P-value:4.68E-5 ID:SGTC_2940|Compound:9060638|FD-Score:5.19|P-value:2.00E-6 ID:SGTC_2966|Compound:9084041|FD-Score:4.46|P-value:3.91E-5 ID:SGTC_3029|Compound:9088683|FD-Score:4.32|P-value:6.51E-5 ID:SGTC_3052|Compound:9092717|FD-Score:4.10|P-value:1.41E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.42|P-value:8.70E-4 ID:SGTC_3250|Compound:9135583|FD-Score:4.45|P-value:4.03E-5 ID:SGTC_3265|Compound:9137266|FD-Score:-3.49|P-value:7.06E-4 ID:SGTC_3317|Compound:9138495|FD-Score:4.93|P-value:6.17E-6 ID:SGTC_6|Compound:0844-0013|FD-Score:6.08|P-value:3.13E-8 ID:SGTC_78|Compound:0336-0123|FD-Score:5.17|P-value:2.17E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:3.73|P-value:4.88E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.71|P-value:5.23E-4 ID:SGTC_315|Compound:k915-0449|FD-Score:4.70|P-value:1.52E-5 ID:SGTC_409|Compound:arachidonic acid|FD-Score:4.16|P-value:1.14E-4 ID:SGTC_411|Compound:enrofloxacin|FD-Score:4.28|P-value:7.61E-5 ID:SGTC_537|Compound:1334-0398|FD-Score:-3.80|P-value:2.52E-4 ID:SGTC_876|Compound:0720-0963|FD-Score:5.66|P-value:2.46E-7 ID:SGTC_919|Compound:tnp00227|FD-Score:3.91|P-value:2.69E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.02|P-value:1.90E-4 ID:SGTC_1206|Compound:3978-0017|FD-Score:6.94|P-value:3.18E-10 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.19|P-value:1.05E-4 ID:SGTC_1937|Compound:st074714|FD-Score:4.99|P-value:4.69E-6 ID:SGTC_2015|Compound:5119600|FD-Score:-7.69|P-value:1.56E-12 ID:SGTC_2024|Compound:5144227|FD-Score:-4.53|P-value:1.78E-5 ID:SGTC_2110|Compound:5483698|FD-Score:3.67|P-value:6.00E-4 ID:SGTC_2117|Compound:5521686|FD-Score:3.59|P-value:7.65E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.74|P-value:3.14E-4 ID:SGTC_2363|Compound:9053361|FD-Score:-4.09|P-value:9.23E-5 ID:SGTC_2366|Compound:9070441|FD-Score:-3.73|P-value:3.27E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.22|P-value:9.33E-5 ID:SGTC_2682|Compound:pyrogallin|FD-Score:6.13|P-value:2.51E-8 ID:SGTC_2842|Compound:9000675|FD-Score:4.41|P-value:4.68E-5 ID:SGTC_2940|Compound:9060638|FD-Score:5.19|P-value:2.00E-6 ID:SGTC_2966|Compound:9084041|FD-Score:4.46|P-value:3.91E-5 ID:SGTC_3029|Compound:9088683|FD-Score:4.32|P-value:6.51E-5 ID:SGTC_3052|Compound:9092717|FD-Score:4.10|P-value:1.41E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.42|P-value:8.70E-4 ID:SGTC_3250|Compound:9135583|FD-Score:4.45|P-value:4.03E-5 ID:SGTC_3265|Compound:9137266|FD-Score:-3.49|P-value:7.06E-4 ID:SGTC_3317|Compound:9138495|FD-Score:4.93|P-value:6.17E-6

Top fitness defect scores for YKR043C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1206 3978-0017 6.94 132.0 µM 3.18E-10 ERG2 8 1.96E-12
2 SGTC_2682 pyrogallin 6.13 40.0 µM 2.51E-8 copper-dependent oxidative stress 3 4.45E-10
3 SGTC_6 0844-0013 6.08 19.8 µM 3.13E-8 14 5.87E-10
4 SGTC_876 0720-0963 5.66 56.8 µM 2.46E-7 4 7.60E-9
5 SGTC_2940 9060638 5.19 45.9 µM 2.00E-6 amide catabolism 6 1.03E-7
6 SGTC_78 0336-0123 5.17 156.8 µM 2.17E-6 10 1.14E-7
7 SGTC_1937 st074714 4.99 47.2 µM 4.69E-6 20 2.97E-7
8 SGTC_3317 9138495 4.93 8.8 µM 6.17E-6 20 4.17E-7
9 SGTC_315 k915-0449 4.70 187.0 µM 1.52E-5 40 1.27E-6
10 SGTC_2966 9084041 4.46 48.0 µM 3.91E-5 21 4.12E-6
11 SGTC_3250 9135583 4.45 49.5 µM 4.03E-5 6 4.29E-6
12 SGTC_2842 9000675 4.41 19.5 µM 4.68E-5 13 5.16E-6
13 SGTC_3029 9088683 4.32 49.5 µM 6.51E-5 20 7.78E-6
14 SGTC_411 enrofloxacin 4.28 100.0 µM 7.61E-5 DNA damage response 7 9.44E-6
15 SGTC_2425 5628681 4.22 7.8 µM 9.33E-5 16 1.22E-5
16 SGTC_1245 0418-0076 4.19 17.2 µM 1.05E-4 ERG2 25 1.41E-5
17 SGTC_409 arachidonic acid 4.16 100.0 µM 1.14E-4 17 1.56E-5
18 SGTC_3052 9092717 4.10 49.5 µM 1.41E-4 23 2.03E-5
19 SGTC_1190 1486-1332 4.02 13.0 µM 1.90E-4 11 2.94E-5
20 SGTC_919 tnp00227 3.91 403.6 nM 2.69E-4 RNA pol III & RNase P/MRP 36 4.53E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 7.95E-13 YNL149C PGA2 het Essential protein required for maturation of Gas1p and Pho8p; involved in protein trafficking; GFP-fusion protein localizes to the ER and YFP-fusion protein to the nuclear envelope-ER network; null mutants have a cell separation defect
0.115 2.56E-11 YLR345W_p YLR345W_p hom Similar to 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase enzymes responsible for the metabolism of fructoso-2,6-bisphosphate; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
0.110 1.58E-10 YEL023C_p YEL023C_p hom Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
0.103 2.46E-9 YML088W UFO1 hom F-box receptor protein, subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitylation by SCF and subsequent degradation
0.094 4.31E-8 YKL146W AVT3 hom Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.093 7.12E-8 YBR025C OLA1 hom P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
0.091 1.40E-7 YDL029W ARP2 het Essential component of the Arp2/3 complex, which is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
0.088 3.14E-7 YIL136W OM45 hom Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress
0.087 4.81E-7 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.085 8.32E-7 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
0.085 8.53E-7 YLR398C SKI2 hom Ski complex component and putative RNA helicase, mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay
0.084 9.81E-7 YOR027W STI1 hom Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
0.082 1.95E-6 YDL048C STP4 hom Protein containing a Kruppel-type zinc-finger domain; has similarity to Stp1p, Stp2p, and Stp3p; predicted to be a transcription factor; relative distribution to the nucleus increases upon DNA replication stress
0.080 3.09E-6 YBR089C-A NHP6B hom High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
0.080 3.86E-6 YNL150W_d YNL150W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; extensive overlap with PGA2/YNL149C, an uncharacterized gene with a proposed role in protein trafficking