YKR053C / YSR3

Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR053C / YSR3

Click on Significant Values for Screen Details ID:SGTC_158|Compound:k038-0087|FD-Score:3.79|P-value:1.10E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.62|P-value:2.05E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.01|P-value:4.66E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:3.59|P-value:2.30E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:-3.35|P-value:5.08E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.85|P-value:8.72E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:4.14|P-value:2.71E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.25|P-value:7.77E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.23|P-value:8.07E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.36|P-value:5.23E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.62|P-value:2.05E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.18|P-value:2.28E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.46|P-value:6.89E-6 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.40|P-value:4.64E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:4.42|P-value:8.11E-6 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.20|P-value:9.06E-4 ID:SGTC_1779|Compound:st039427|FD-Score:-3.56|P-value:2.43E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.32|P-value:6.04E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.42|P-value:4.33E-4 ID:SGTC_1949|Compound:st076024|FD-Score:-3.49|P-value:3.16E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.29|P-value:1.39E-5 ID:SGTC_2043|Compound:5101342|FD-Score:5.48|P-value:4.33E-8 ID:SGTC_2132|Compound:5317594|FD-Score:-3.44|P-value:3.74E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-3.19|P-value:8.76E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.78|P-value:1.11E-4 ID:SGTC_2346|Compound:9031566|FD-Score:4.05|P-value:3.85E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.50|P-value:3.26E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.56|P-value:2.62E-4 ID:SGTC_2966|Compound:9084041|FD-Score:8.23|P-value:4.78E-16 ID:SGTC_3010|Compound:9080089|FD-Score:3.30|P-value:6.47E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.32|P-value:5.98E-4 ID:SGTC_3193|Compound:9111931|FD-Score:3.33|P-value:5.77E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.64|P-value:1.80E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.89|P-value:9.19E-7 ID:SGTC_3332|Compound:9141069|FD-Score:4.41|P-value:8.29E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:3.79|P-value:1.10E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:3.62|P-value:2.05E-4 ID:SGTC_271|Compound:rotenone|FD-Score:4.01|P-value:4.66E-5 ID:SGTC_404|Compound:fumagillin|FD-Score:3.59|P-value:2.30E-4 ID:SGTC_685|Compound:1278-0033|FD-Score:-3.35|P-value:5.08E-4 ID:SGTC_690|Compound:glyoxal|FD-Score:3.85|P-value:8.72E-5 ID:SGTC_1051|Compound:3253-3903|FD-Score:4.14|P-value:2.71E-5 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.25|P-value:7.77E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.23|P-value:8.07E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.36|P-value:5.23E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:3.62|P-value:2.05E-4 ID:SGTC_1245|Compound:0418-0076|FD-Score:4.18|P-value:2.28E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.46|P-value:6.89E-6 ID:SGTC_1421|Compound:4029-0249|FD-Score:3.40|P-value:4.64E-4 ID:SGTC_1424|Compound:4048-1941|FD-Score:4.42|P-value:8.11E-6 ID:SGTC_1473|Compound:k844-0725|FD-Score:3.20|P-value:9.06E-4 ID:SGTC_1779|Compound:st039427|FD-Score:-3.56|P-value:2.43E-4 ID:SGTC_1824|Compound:st053723|FD-Score:3.32|P-value:6.04E-4 ID:SGTC_1864|Compound:st057645|FD-Score:3.42|P-value:4.33E-4 ID:SGTC_1949|Compound:st076024|FD-Score:-3.49|P-value:3.16E-4 ID:SGTC_2019|Compound:4016004|FD-Score:4.29|P-value:1.39E-5 ID:SGTC_2043|Compound:5101342|FD-Score:5.48|P-value:4.33E-8 ID:SGTC_2132|Compound:5317594|FD-Score:-3.44|P-value:3.74E-4 ID:SGTC_2171|Compound:5739187|FD-Score:-3.19|P-value:8.76E-4 ID:SGTC_2340|Compound:7987427|FD-Score:3.78|P-value:1.11E-4 ID:SGTC_2346|Compound:9031566|FD-Score:4.05|P-value:3.85E-5 ID:SGTC_2485|Compound:5633444|FD-Score:3.69|P-value:1.57E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.50|P-value:3.26E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.56|P-value:2.62E-4 ID:SGTC_2966|Compound:9084041|FD-Score:8.23|P-value:4.78E-16 ID:SGTC_3010|Compound:9080089|FD-Score:3.30|P-value:6.47E-4 ID:SGTC_3189|Compound:9110682|FD-Score:3.32|P-value:5.98E-4 ID:SGTC_3193|Compound:9111931|FD-Score:3.33|P-value:5.77E-4 ID:SGTC_3311|Compound:9131146|FD-Score:-3.64|P-value:1.80E-4 ID:SGTC_3323|Compound:9138564|FD-Score:4.89|P-value:9.19E-7 ID:SGTC_3332|Compound:9141069|FD-Score:4.41|P-value:8.29E-6

Top fitness defect scores for YKR053C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2966 9084041 8.23 48.0 µM 4.78E-16 3 9.74E-17
2 SGTC_2043 5101342 5.48 149.0 µM 4.33E-8 SWF1 & branched chain AA biosynthesis 5 2.08E-8
3 SGTC_3323 9138564 4.89 53.9 µM 9.19E-7 7 5.09E-7
4 SGTC_1328 1441-0025 4.46 69.2 µM 6.89E-6 2 4.18E-6
5 SGTC_1424 4048-1941 4.42 275.0 µM 8.11E-6 16 4.96E-6
6 SGTC_3332 9141069 4.41 11.2 µM 8.29E-6 8 5.08E-6
7 SGTC_2019 4016004 4.29 108.0 µM 1.39E-5 19 8.75E-6
8 SGTC_1245 0418-0076 4.18 17.2 µM 2.28E-5 ERG2 26 1.46E-5
9 SGTC_1051 3253-3903 4.14 78.0 µM 2.71E-5 20 1.75E-5
10 SGTC_2346 9031566 4.05 143.8 µM 3.85E-5 Golgi 14 2.53E-5
11 SGTC_271 rotenone 4.01 740.0 µM 4.66E-5 21 3.09E-5
12 SGTC_690 glyoxal 3.85 20.3 µM 8.72E-5 85 5.96E-5
13 SGTC_158 k038-0087 3.79 26.4 µM 1.10E-4 ubiquinone biosynthesis & proteasome 151 7.58E-5
14 SGTC_2340 7987427 3.78 183.4 µM 1.11E-4 39 7.69E-5
15 SGTC_2485 5633444 3.69 17.8 µM 1.57E-4 redox potentiating 55 1.10E-4
16 SGTC_1121 fusaric acid 3.62 630.5 nM 2.05E-4 43 1.45E-4
17 SGTC_268 camptothecin 3.62 39.6 µM 2.05E-4 DNA damage response 85 1.46E-4
18 SGTC_404 fumagillin 3.59 50.0 µM 2.30E-4 30 1.65E-4
19 SGTC_2952 9073731 3.56 13.8 µM 2.62E-4 tubulin folding & SWR complex 59 1.89E-4
20 SGTC_2612 dictamnine 3.50 15.5 µM 3.26E-4 RNA pol III & RNase P/MRP 60 2.36E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.126 2.43E-13 YPL192C PRM3 hom Pheromone-regulated protein required for nuclear envelope fusion during karyogamy; localizes to the outer face of the nuclear membrane; interacts with Kar5p at the spindle pole body
0.113 4.51E-11 YKL146W AVT3 hom Vacuolar transporter, exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.109 2.85E-10 YDR181C SAS4 hom Subunit of the SAS complex (Sas2p, Sas4p, Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
0.106 7.77E-10 YDL034W_d YDL034W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
0.101 5.01E-9 YML098W TAF13 het TFIID subunit (19 kDa), involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
0.096 2.52E-8 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.093 7.28E-8 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.089 2.32E-7 YEL071W DLD3 hom D-lactate dehydrogenase, part of the retrograde regulon which consists of genes whose expression is stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source, located in the cytoplasm
0.089 2.59E-7 YOR057W SGT1 het Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
0.089 2.77E-7 YKL221W MCH2 hom Protein with similarity to mammalian monocarboxylate permeases, which are involved in transport of monocarboxylic acids across the plasma membrane; mutant is not deficient in monocarboxylate transport
0.088 3.39E-7 YNL103W MET4 het Leucine-zipper transcriptional activator, responsible for the regulation of the sulfur amino acid pathway, requires different combinations of the auxiliary factors Cbf1p, Met28p, Met31p and Met32p
0.087 4.26E-7 YGR190C_d YGR190C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
0.087 4.59E-7 YLR378C SEC61 het Essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms a channel for SRP-dependent protein import and retrograde transport of misfolded proteins out of the ER; with Sec63 complex allows SRP-independent protein import into ER
0.085 8.17E-7 YDR315C IPK1 hom Inositol 1,3,4,5,6-pentakisphosphate 2-kinase, nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable
0.084 1.25E-6 YLR233C EST1 hom TLC1 RNA-associated factor involved in telomere length regulation as recruitment subunit of telomerase; has G-quadruplex promoting activity that is required for telomere elongation; possible role in activating telomere-bound Est2p-TLC1-RNA