YKR056W / TRM2

tRNA methyltransferase, 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; endo-exonuclease with a role in DNA repair

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR056W / TRM2

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:4.44|P-value:2.22E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.33|P-value:2.87E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.30|P-value:3.21E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.42|P-value:2.07E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.31|P-value:3.12E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:5.46|P-value:8.24E-9 ID:SGTC_215|Compound:1287-0124|FD-Score:3.52|P-value:1.35E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.21|P-value:4.54E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.11|P-value:6.40E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-4.24|P-value:5.92E-6 ID:SGTC_611|Compound:3453-1652|FD-Score:3.08|P-value:7.32E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.87|P-value:3.12E-5 ID:SGTC_764|Compound:1951-2361|FD-Score:3.65|P-value:7.96E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.86|P-value:3.28E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.46|P-value:1.70E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.31|P-value:3.05E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.05|P-value:8.18E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:4.22|P-value:6.53E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.76|P-value:5.05E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:-4.03|P-value:1.57E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.75|P-value:5.23E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.49|P-value:1.56E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.24|P-value:4.06E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.73|P-value:5.65E-5 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.69|P-value:6.33E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.51|P-value:1.45E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.00|P-value:9.60E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.01|P-value:9.26E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.19|P-value:4.84E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.86|P-value:3.34E-5 ID:SGTC_1731|Compound:st035964|FD-Score:4.07|P-value:1.30E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:1.23E-6 ID:SGTC_1747|Compound:st037496|FD-Score:-4.02|P-value:1.61E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.19|P-value:4.93E-4 ID:SGTC_1868|Compound:st057644|FD-Score:3.37|P-value:2.44E-4 ID:SGTC_1954|Compound:st077032|FD-Score:3.35|P-value:2.64E-4 ID:SGTC_2218|Compound:6965428|FD-Score:3.06|P-value:7.82E-4 ID:SGTC_2241|Compound:6772625|FD-Score:4.06|P-value:1.33E-5 ID:SGTC_2260|Compound:7964312|FD-Score:3.30|P-value:3.28E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.88|P-value:2.98E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.43|P-value:1.99E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.27|P-value:3.56E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.26|P-value:3.77E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.72|P-value:6.15E-5 ID:SGTC_2456|Compound:5770359|FD-Score:-2.99|P-value:9.96E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.41|P-value:2.09E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_2745|Compound:rifampicin|FD-Score:3.48|P-value:1.58E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.68|P-value:6.66E-7 ID:SGTC_2806|Compound:7952793|FD-Score:-5.63|P-value:2.92E-9 ID:SGTC_2812|Compound:7970281|FD-Score:5.94|P-value:4.02E-10 ID:SGTC_2823|Compound:7987624|FD-Score:3.81|P-value:4.07E-5 ID:SGTC_2834|Compound:9001755|FD-Score:3.78|P-value:4.68E-5 ID:SGTC_2841|Compound:9004761|FD-Score:3.40|P-value:2.22E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.07|P-value:1.28E-5 ID:SGTC_3037|Compound:9091026|FD-Score:3.46|P-value:1.75E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.08|P-value:7.24E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-4.53|P-value:1.43E-6 ID:SGTC_3234|Compound:9132280|FD-Score:3.68|P-value:7.08E-5 ID:SGTC_3302|Compound:9125395|FD-Score:3.30|P-value:3.28E-4 ID:SGTC_3353|Compound:9151644|FD-Score:3.45|P-value:1.84E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:4.44|P-value:2.22E-6 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.33|P-value:2.87E-4 ID:SGTC_79|Compound:0052-0007|FD-Score:3.30|P-value:3.21E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.42|P-value:2.07E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.31|P-value:3.12E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:5.46|P-value:8.24E-9 ID:SGTC_215|Compound:1287-0124|FD-Score:3.52|P-value:1.35E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:3.21|P-value:4.54E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:3.11|P-value:6.40E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:-4.24|P-value:5.92E-6 ID:SGTC_611|Compound:3453-1652|FD-Score:3.08|P-value:7.32E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.87|P-value:3.12E-5 ID:SGTC_764|Compound:1951-2361|FD-Score:3.65|P-value:7.96E-5 ID:SGTC_768|Compound:0384-0033|FD-Score:3.86|P-value:3.28E-5 ID:SGTC_783|Compound:0848-0141|FD-Score:3.46|P-value:1.70E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.31|P-value:3.05E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:-3.05|P-value:8.18E-4 ID:SGTC_1111|Compound:tnp00062|FD-Score:4.22|P-value:6.53E-6 ID:SGTC_1155|Compound:4088-0394|FD-Score:3.76|P-value:5.05E-5 ID:SGTC_1234|Compound:0326-0494|FD-Score:-4.03|P-value:1.57E-5 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.75|P-value:5.23E-5 ID:SGTC_1263|Compound:0774-1208|FD-Score:-3.49|P-value:1.56E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:3.51|P-value:1.42E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.24|P-value:4.06E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:3.73|P-value:5.65E-5 ID:SGTC_1461|Compound:k072-0203|FD-Score:4.69|P-value:6.33E-7 ID:SGTC_1520|Compound:flindersine|FD-Score:-3.51|P-value:1.45E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.00|P-value:9.60E-4 ID:SGTC_1539|Compound:benzyladenosine|FD-Score:3.01|P-value:9.26E-4 ID:SGTC_1616|Compound:st002734|FD-Score:3.19|P-value:4.84E-4 ID:SGTC_1701|Compound:st028638|FD-Score:3.86|P-value:3.34E-5 ID:SGTC_1731|Compound:st035964|FD-Score:4.07|P-value:1.30E-5 ID:SGTC_1745|Compound:st081495|FD-Score:4.56|P-value:1.23E-6 ID:SGTC_1747|Compound:st037496|FD-Score:-4.02|P-value:1.61E-5 ID:SGTC_1818|Compound:st052136|FD-Score:3.19|P-value:4.93E-4 ID:SGTC_1868|Compound:st057644|FD-Score:3.37|P-value:2.44E-4 ID:SGTC_1954|Compound:st077032|FD-Score:3.35|P-value:2.64E-4 ID:SGTC_2218|Compound:6965428|FD-Score:3.06|P-value:7.82E-4 ID:SGTC_2241|Compound:6772625|FD-Score:4.06|P-value:1.33E-5 ID:SGTC_2260|Compound:7964312|FD-Score:3.30|P-value:3.28E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.88|P-value:2.98E-5 ID:SGTC_2321|Compound:7390090|FD-Score:3.43|P-value:1.99E-4 ID:SGTC_2375|Compound:9072683|FD-Score:3.27|P-value:3.56E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.26|P-value:3.77E-4 ID:SGTC_2455|Compound:5377712|FD-Score:-3.72|P-value:6.15E-5 ID:SGTC_2456|Compound:5770359|FD-Score:-2.99|P-value:9.96E-4 ID:SGTC_2482|Compound:5981709|FD-Score:3.41|P-value:2.09E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_2745|Compound:rifampicin|FD-Score:3.48|P-value:1.58E-4 ID:SGTC_2804|Compound:7954240|FD-Score:4.68|P-value:6.66E-7 ID:SGTC_2806|Compound:7952793|FD-Score:-5.63|P-value:2.92E-9 ID:SGTC_2812|Compound:7970281|FD-Score:5.94|P-value:4.02E-10 ID:SGTC_2823|Compound:7987624|FD-Score:3.81|P-value:4.07E-5 ID:SGTC_2834|Compound:9001755|FD-Score:3.78|P-value:4.68E-5 ID:SGTC_2841|Compound:9004761|FD-Score:3.40|P-value:2.22E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.07|P-value:1.28E-5 ID:SGTC_3037|Compound:9091026|FD-Score:3.46|P-value:1.75E-4 ID:SGTC_3042|Compound:9091578|FD-Score:3.08|P-value:7.24E-4 ID:SGTC_3050|Compound:9093513|FD-Score:-4.53|P-value:1.43E-6 ID:SGTC_3234|Compound:9132280|FD-Score:3.68|P-value:7.08E-5 ID:SGTC_3302|Compound:9125395|FD-Score:3.30|P-value:3.28E-4 ID:SGTC_3353|Compound:9151644|FD-Score:3.45|P-value:1.84E-4

Top fitness defect scores for YKR056W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2812 7970281 5.94 58.4 µM 4.02E-10 3 1.40E-9
2 SGTC_166 k072-0058 5.46 166.4 µM 8.24E-9 DNA intercalators 10 2.38E-8
3 SGTC_1461 k072-0203 4.69 53.8 µM 6.33E-7 4 1.39E-6
4 SGTC_2804 7954240 4.68 19.5 µM 6.66E-7 20 1.46E-6
5 SGTC_1745 st081495 4.56 74.0 µM 1.23E-6 RNA processing & uracil transport 58 2.60E-6
6 SGTC_49 0717-0965 4.44 32.1 µM 2.22E-6 60S ribosome export 13 4.52E-6
7 SGTC_1111 tnp00062 4.22 510.7 nM 6.53E-6 6 1.24E-5
8 SGTC_2913 7966556 4.07 36.6 µM 1.28E-5 60S ribosome export 8 2.34E-5
9 SGTC_1731 st035964 4.07 63.0 µM 1.30E-5 26 2.37E-5
10 SGTC_2241 6772625 4.06 37.8 µM 1.33E-5 21 2.42E-5
11 SGTC_2295 7971645 3.88 200.0 µM 2.98E-5 24 5.17E-5
12 SGTC_756 0508-1999 3.87 28.3 µM 3.12E-5 RPP1 & pyrimidine depletion 29 5.40E-5
13 SGTC_768 0384-0033 3.86 77.3 µM 3.28E-5 fatty acid desaturase (OLE1) 86 5.67E-5
14 SGTC_1701 st028638 3.86 40.4 µM 3.34E-5 16 5.75E-5
15 SGTC_2823 7987624 3.81 58.4 µM 4.07E-5 33 6.92E-5
16 SGTC_2834 9001755 3.78 58.4 µM 4.68E-5 44 7.90E-5
17 SGTC_1155 4088-0394 3.76 48.2 µM 5.05E-5 33 8.49E-5
18 SGTC_1458 k015-0034 3.73 6.3 µM 5.65E-5 19 9.43E-5
19 SGTC_3234 9132280 3.68 49.5 µM 7.08E-5 26 1.17E-4
20 SGTC_764 1951-2361 3.65 146.0 µM 7.96E-5 47 1.30E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.097 1.83E-8 YDR077W SED1 hom Major stress-induced structural GPI-cell wall glycoprotein in stationary-phase cells, associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites
0.095 3.12E-8 YMR225C MRPL44 hom Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
0.092 1.06E-7 YDR395W SXM1 hom Nuclear transport factor (karyopherin) involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
0.091 1.14E-7 YDR292C SRP101 het Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
0.089 2.44E-7 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.088 3.03E-7 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.087 3.93E-7 YLR165C PUS5 hom Pseudouridine synthase, catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
0.087 4.47E-7 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.085 7.79E-7 YLR277C YSH1 het Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
0.085 8.78E-7 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.083 1.58E-6 YDR240C SNU56 het Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
0.082 1.86E-6 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.081 2.61E-6 YGR146C ECL1 hom Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
0.080 3.27E-6 YDR353W TRR1 het Cytoplasmic thioredoxin reductase; key regulatory enzyme that determines the redox state of the thioredoxin system, which acts as a disulfide reductase system and protects cells against both oxidative and reductive stress; protein abundance increases in response to DNA replication stress; TRR1 has a paralog, TRR2, that arose from the whole genome duplication
0.079 4.15E-6 YLR438C-A LSM3 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress