YKR059W / TIF1

Translation initiation factor eIF4A; DEA(D/H)-box RNA helicase that couples ATPase activity to RNA binding and unwinding; forms a dumbbell structure of two compact domains connected by a linker; interacts with eIF4G; protein abundance increases in response to DNA replication stress; TIF1 has a paralog, TIF2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR059W / TIF1

Click on Significant Values for Screen Details ID:SGTC_71|Compound:0417-1750|FD-Score:9.31|P-value:2.79E-21 ID:SGTC_78|Compound:0336-0123|FD-Score:4.33|P-value:6.13E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:5.20|P-value:7.74E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:4.97|P-value:2.61E-7 ID:SGTC_349|Compound:k015-0014|FD-Score:3.25|P-value:5.13E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.91|P-value:3.51E-7 ID:SGTC_534|Compound:k292-0788|FD-Score:3.52|P-value:1.93E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:3.08|P-value:9.50E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.71|P-value:9.13E-5 ID:SGTC_755|Compound:1995-0008|FD-Score:3.47|P-value:2.33E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.90|P-value:4.11E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:-4.20|P-value:1.09E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.15|P-value:7.45E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:5.25|P-value:5.70E-8 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.09|P-value:9.14E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.09|P-value:9.27E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.13|P-value:7.82E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.23|P-value:5.52E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.80|P-value:6.46E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.39|P-value:3.04E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-3.53|P-value:1.81E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-3.62|P-value:1.26E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.74|P-value:7.96E-5 ID:SGTC_2240|Compound:6690706|FD-Score:3.75|P-value:7.76E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.45|P-value:2.42E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:7.30|P-value:8.43E-14 ID:SGTC_2411|Compound:st077133|FD-Score:-5.41|P-value:2.30E-8 ID:SGTC_2416|Compound:tofa|FD-Score:-4.53|P-value:2.35E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.06|P-value:2.01E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.66|P-value:5.44E-9 ID:SGTC_2444|Compound:5485484|FD-Score:-3.85|P-value:4.96E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.75|P-value:7.42E-5 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:3.32|P-value:4.01E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.56|P-value:1.64E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.17|P-value:6.72E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.11|P-value:8.60E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.21|P-value:1.05E-5 ID:SGTC_3014|Compound:9081533|FD-Score:3.86|P-value:4.98E-5 ID:SGTC_3102|Compound:9121669|FD-Score:-3.06|P-value:9.80E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.09|P-value:8.91E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-3.52|P-value:1.87E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.09|P-value:8.89E-4 ID:SGTC_3242|Compound:9133940|FD-Score:4.32|P-value:6.43E-6 ID:SGTC_71|Compound:0417-1750|FD-Score:9.31|P-value:2.79E-21 ID:SGTC_78|Compound:0336-0123|FD-Score:4.33|P-value:6.13E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:5.20|P-value:7.74E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:4.97|P-value:2.61E-7 ID:SGTC_349|Compound:k015-0014|FD-Score:3.25|P-value:5.13E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:-4.91|P-value:3.51E-7 ID:SGTC_534|Compound:k292-0788|FD-Score:3.52|P-value:1.93E-4 ID:SGTC_645|Compound:0845-0943|FD-Score:3.08|P-value:9.50E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:3.71|P-value:9.13E-5 ID:SGTC_755|Compound:1995-0008|FD-Score:3.47|P-value:2.33E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:3.90|P-value:4.11E-5 ID:SGTC_907|Compound:3448-6986|FD-Score:-4.20|P-value:1.09E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:3.15|P-value:7.45E-4 ID:SGTC_1103|Compound:duloxetine|FD-Score:5.25|P-value:5.70E-8 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.09|P-value:9.14E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.09|P-value:9.27E-4 ID:SGTC_1609|Compound:st001574|FD-Score:3.13|P-value:7.82E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.23|P-value:5.52E-4 ID:SGTC_1875|Compound:nocodazole|FD-Score:4.80|P-value:6.46E-7 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.39|P-value:3.04E-4 ID:SGTC_1884|Compound:5633444|FD-Score:-3.53|P-value:1.81E-4 ID:SGTC_1950|Compound:st077106|FD-Score:-3.62|P-value:1.26E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.74|P-value:7.96E-5 ID:SGTC_2240|Compound:6690706|FD-Score:3.75|P-value:7.76E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:-3.45|P-value:2.42E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:7.30|P-value:8.43E-14 ID:SGTC_2411|Compound:st077133|FD-Score:-5.41|P-value:2.30E-8 ID:SGTC_2416|Compound:tofa|FD-Score:-4.53|P-value:2.35E-6 ID:SGTC_2418|Compound:pentamidine|FD-Score:-4.06|P-value:2.01E-5 ID:SGTC_2419|Compound:paclitaxel|FD-Score:5.66|P-value:5.44E-9 ID:SGTC_2444|Compound:5485484|FD-Score:-3.85|P-value:4.96E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.75|P-value:7.42E-5 ID:SGTC_2728|Compound:chlorpromazine|FD-Score:3.32|P-value:4.01E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.56|P-value:1.64E-4 ID:SGTC_2948|Compound:9070189|FD-Score:-3.17|P-value:6.72E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.11|P-value:8.60E-4 ID:SGTC_2981|Compound:9013952|FD-Score:4.21|P-value:1.05E-5 ID:SGTC_3014|Compound:9081533|FD-Score:3.86|P-value:4.98E-5 ID:SGTC_3102|Compound:9121669|FD-Score:-3.06|P-value:9.80E-4 ID:SGTC_3137|Compound:9097352|FD-Score:-3.09|P-value:8.91E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-3.52|P-value:1.87E-4 ID:SGTC_3194|Compound:9111903|FD-Score:-3.09|P-value:8.89E-4 ID:SGTC_3242|Compound:9133940|FD-Score:4.32|P-value:6.43E-6

Top fitness defect scores for YKR059W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_71 0417-1750 9.31 47.8 µM 2.79E-21 Golgi 6 6.49E-21
2 SGTC_2408 sulbentine 7.30 18.4 µM 8.43E-14 14 1.41E-13
3 SGTC_2419 paclitaxel 5.66 166.5 µM 5.44E-9 6 7.41E-9
4 SGTC_1103 duloxetine 5.25 36.1 µM 5.70E-8 5 7.45E-8
5 SGTC_91 0417-1751 5.20 58.4 µM 7.74E-8 Golgi 21 1.00E-7
6 SGTC_309 k408-0051 4.97 1.5 µM 2.61E-7 29 3.32E-7
7 SGTC_1875 nocodazole 4.80 6.0 µM 6.46E-7 tubulin folding & SWR complex 41 8.06E-7
8 SGTC_78 0336-0123 4.33 156.8 µM 6.13E-6 17 7.35E-6
9 SGTC_3242 9133940 4.32 49.5 µM 6.43E-6 5 7.70E-6
10 SGTC_2981 9013952 4.21 39.0 µM 1.05E-5 35 1.25E-5
11 SGTC_756 0508-1999 3.90 28.3 µM 4.11E-5 RPP1 & pyrimidine depletion 27 4.76E-5
12 SGTC_3014 9081533 3.86 49.5 µM 4.98E-5 12 5.74E-5
13 SGTC_2240 6690706 3.75 185.9 µM 7.76E-5 21 8.88E-5
14 SGTC_2154 5570129 3.74 200.0 µM 7.96E-5 22 9.11E-5
15 SGTC_695 3505-6186 3.71 243.0 µM 9.13E-5 30 1.04E-4
16 SGTC_2942 9052777 3.56 62.2 µM 1.64E-4 48 1.85E-4
17 SGTC_534 k292-0788 3.52 63.4 µM 1.93E-4 mitochondrial processes 45 2.18E-4
18 SGTC_755 1995-0008 3.47 37.3 µM 2.33E-4 34 2.62E-4
19 SGTC_2728 chlorpromazine 3.32 9.2 µM 4.01E-4 8 4.46E-4
20 SGTC_349 k015-0014 3.25 24.0 µM 5.13E-4 47 5.68E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.216 8.29E-37 YDR439W LRS4 hom Nucleolar protein that forms a complex with Csm1p, and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
0.147 1.02E-17 YJL153C INO1 hom Inositol-3-phosphate synthase, involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
0.142 1.43E-16 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.140 3.77E-16 YBR294W SUL1 hom High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
0.137 1.33E-15 YGR049W SCM4 hom Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation
0.136 2.76E-15 YDL069C CBS1 hom Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
0.127 1.59E-13 YDL037C BSC1 hom Protein of unconfirmed function, similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
0.126 2.58E-13 YGR039W_d YGR039W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the Autonomously Replicating Sequence ARS722
0.121 2.26E-12 YOL053W AIM39 hom Putative protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss
0.116 1.67E-11 YNL334C SNO2 hom Protein of unknown function, nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
0.113 4.94E-11 YMR146C TIF34 het eIF3i subunit of the core complex of translation initiation factor 3 (eIF3), which is essential for translation; stimulates rate of ribosomal scanning during translation reinitiation
0.111 1.08E-10 YGR289C MAL11 hom Inducible high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
0.105 8.99E-10 YGR034W RPL26B hom Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
0.104 1.57E-9 YKL025C PAN3 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.103 2.22E-9 YPL241C CIN2 hom GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; deletion complemented by human GAP, retinitis pigmentosa 2