YKR062W / TFA2

TFIIE small subunit, involved in RNA polymerase II transcription initiation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR062W / TFA2

Click on Significant Values for Screen Details ID:SGTC_155|Compound:k072-0202|FD-Score:3.67|P-value:2.69E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-4.39|P-value:1.63E-5 ID:SGTC_641|Compound:4092-0595|FD-Score:3.66|P-value:2.82E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.06|P-value:6.36E-5 ID:SGTC_972|Compound:1319-0219|FD-Score:3.62|P-value:3.23E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.82|P-value:1.50E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.96|P-value:9.52E-5 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.32|P-value:8.34E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.29|P-value:9.23E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.63|P-value:6.24E-6 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.27|P-value:2.82E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.23|P-value:3.38E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.98|P-value:8.80E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.82|P-value:1.59E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.49|P-value:5.05E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.30|P-value:8.99E-4 ID:SGTC_1526|Compound:st051518|FD-Score:5.05|P-value:9.53E-7 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.51|P-value:4.63E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.39|P-value:7.11E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.46|P-value:5.52E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.71|P-value:3.50E-8 ID:SGTC_1646|Compound:st011709|FD-Score:7.28|P-value:3.27E-12 ID:SGTC_1682|Compound:st012968|FD-Score:-3.81|P-value:1.52E-4 ID:SGTC_1778|Compound:st046819|FD-Score:3.43|P-value:6.10E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.74|P-value:2.11E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-4.09|P-value:5.44E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.96|P-value:9.48E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:5.34|P-value:2.37E-7 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.37|P-value:7.40E-4 ID:SGTC_2643|Compound:anethole|FD-Score:4.40|P-value:1.67E-5 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.78|P-value:1.83E-4 ID:SGTC_2717|Compound:st081029|FD-Score:-3.51|P-value:4.40E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-6.01|P-value:6.35E-9 ID:SGTC_2797|Compound:7859098|FD-Score:-4.67|P-value:4.84E-6 ID:SGTC_2814|Compound:7977482|FD-Score:-3.69|P-value:2.40E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-4.57|P-value:7.74E-6 ID:SGTC_2830|Compound:7992007|FD-Score:6.15|P-value:3.24E-9 ID:SGTC_2833|Compound:9001315|FD-Score:4.97|P-value:1.35E-6 ID:SGTC_3053|Compound:9095903|FD-Score:3.49|P-value:5.10E-4 ID:SGTC_3184|Compound:9108758|FD-Score:3.52|P-value:4.50E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-3.43|P-value:5.72E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.38|P-value:7.28E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.28|P-value:9.50E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:3.67|P-value:2.69E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:-4.39|P-value:1.63E-5 ID:SGTC_641|Compound:4092-0595|FD-Score:3.66|P-value:2.82E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:4.06|P-value:6.36E-5 ID:SGTC_972|Compound:1319-0219|FD-Score:3.62|P-value:3.23E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:-3.82|P-value:1.50E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.96|P-value:9.52E-5 ID:SGTC_1154|Compound:4088-0362|FD-Score:-3.32|P-value:8.34E-4 ID:SGTC_1162|Compound:k048-0130|FD-Score:-3.29|P-value:9.23E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:4.63|P-value:6.24E-6 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.27|P-value:2.82E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:4.23|P-value:3.38E-5 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.98|P-value:8.80E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.82|P-value:1.59E-4 ID:SGTC_1352|Compound:1496-0991|FD-Score:3.49|P-value:5.05E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.30|P-value:8.99E-4 ID:SGTC_1526|Compound:st051518|FD-Score:5.05|P-value:9.53E-7 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.51|P-value:4.63E-4 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.39|P-value:7.11E-4 ID:SGTC_1610|Compound:st001799|FD-Score:3.46|P-value:5.52E-4 ID:SGTC_1635|Compound:st007035|FD-Score:5.71|P-value:3.50E-8 ID:SGTC_1646|Compound:st011709|FD-Score:7.28|P-value:3.27E-12 ID:SGTC_1682|Compound:st012968|FD-Score:-3.81|P-value:1.52E-4 ID:SGTC_1778|Compound:st046819|FD-Score:3.43|P-value:6.10E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.74|P-value:2.11E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-4.09|P-value:5.44E-5 ID:SGTC_2083|Compound:5306046|FD-Score:3.96|P-value:9.48E-5 ID:SGTC_2405|Compound:ticlatone|FD-Score:5.34|P-value:2.37E-7 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.37|P-value:7.40E-4 ID:SGTC_2643|Compound:anethole|FD-Score:4.40|P-value:1.67E-5 ID:SGTC_2654|Compound:protoveratrine a|FD-Score:3.78|P-value:1.83E-4 ID:SGTC_2717|Compound:st081029|FD-Score:-3.51|P-value:4.40E-4 ID:SGTC_2794|Compound:7785382|FD-Score:-6.01|P-value:6.35E-9 ID:SGTC_2797|Compound:7859098|FD-Score:-4.67|P-value:4.84E-6 ID:SGTC_2814|Compound:7977482|FD-Score:-3.69|P-value:2.40E-4 ID:SGTC_2829|Compound:7992743|FD-Score:-4.57|P-value:7.74E-6 ID:SGTC_2830|Compound:7992007|FD-Score:6.15|P-value:3.24E-9 ID:SGTC_2833|Compound:9001315|FD-Score:4.97|P-value:1.35E-6 ID:SGTC_3053|Compound:9095903|FD-Score:3.49|P-value:5.10E-4 ID:SGTC_3184|Compound:9108758|FD-Score:3.52|P-value:4.50E-4 ID:SGTC_3213|Compound:9129884|FD-Score:-3.43|P-value:5.72E-4 ID:SGTC_3235|Compound:9132602|FD-Score:3.38|P-value:7.28E-4 ID:SGTC_3314|Compound:9135860|FD-Score:-3.28|P-value:9.50E-4

Top fitness defect scores for YKR062W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1646 st011709 7.28 75.6 µM 3.27E-12 1 1.71E-13 1.42
2 SGTC_2830 7992007 6.15 71.4 µM 3.24E-9 1 3.84E-10 0.58
3 SGTC_1635 st007035 5.71 42.4 µM 3.50E-8 6 5.51E-9 0.02
4 SGTC_2405 ticlatone 5.34 3.5 µM 2.37E-7 10 4.67E-8 0.16
5 SGTC_1526 st051518 5.05 75.0 µM 9.53E-7 3 2.21E-7 0.54
6 SGTC_2833 9001315 4.97 45.5 µM 1.35E-6 amide catabolism 5 3.27E-7 0.76
7 SGTC_1188 1403-0014 4.63 4.6 µM 6.24E-6 mitochondrial stress 9 1.81E-6 0.12
8 SGTC_2643 anethole 4.40 100.0 µM 1.67E-5 1 5.42E-6 0.46
9 SGTC_1190 1486-1332 4.27 13.0 µM 2.82E-5 3 9.74E-6 0.44
10 SGTC_1239 0337-0584 4.23 35.4 µM 3.38E-5 unfolded protein response 27 1.19E-5 0.02
11 SGTC_970 1315-0089 4.06 19.5 µM 6.36E-5 RSC & ERG11 9 2.41E-5 0.00
12 SGTC_1312 1218-2183 3.98 165.0 µM 8.80E-5 1 3.47E-5 0.44
13 SGTC_2083 5306046 3.96 65.9 µM 9.48E-5 4 3.76E-5 0.55
14 SGTC_1072 oxiconazole 3.96 14.9 nM 9.52E-5 ergosterol depletion effects on membrane 20 3.78E-5 0.20
15 SGTC_1347 1493-0251 3.82 600.0 nM 1.59E-4 mitochondrial response to ROS 7 6.69E-5 0.22
16 SGTC_2654 protoveratrine a 3.78 100.0 µM 1.83E-4 6 7.85E-5 0.21
17 SGTC_1843 st055809 3.74 45.5 µM 2.11E-4 9 9.19E-5 0.15
18 SGTC_155 k072-0202 3.67 43.6 µM 2.69E-4 8 1.20E-4 0.05
19 SGTC_641 4092-0595 3.66 30.0 µM 2.82E-4 13 1.27E-4 0.03
20 SGTC_972 1319-0219 3.62 195.0 µM 3.23E-4 9 1.48E-4 0.22

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.159 2.47E-20 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.153 5.17E-19 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.152 7.05E-19 YNR018W RCF2 hom Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
0.134 7.37E-15 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.129 5.96E-14 YLR054C OSW2 hom Protein of unknown function proposed to be involved in the assembly of the spore wall
0.127 1.82E-13 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.118 8.42E-12 YPL189C-A COA2 hom Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
0.117 1.34E-11 YGR076C MRPL25 hom Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
0.114 4.30E-11 YJL034W KAR2 het ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
0.113 4.63E-11 YKL155C RSM22 hom Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent methyltransferase
0.113 5.60E-11 YGL191W COX13 hom Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
0.113 5.79E-11 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.108 3.08E-10 YGR183C QCR9 hom Subunit 9 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
0.104 1.76E-9 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.097 2.18E-8 YCL044C MGR1 hom Subunit of the mitochondrial (mt) i-AAA protease supercomplex, which degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA