YKR064W / OAF3

Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR064W / OAF3

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.49|P-value:4.37E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.90|P-value:9.67E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:7.59|P-value:1.94E-13 ID:SGTC_78|Compound:0336-0123|FD-Score:5.47|P-value:8.79E-8 ID:SGTC_144|Compound:4161-2749|FD-Score:4.05|P-value:5.46E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:6.34|P-value:6.82E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:3.70|P-value:2.05E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:3.25|P-value:9.64E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.27|P-value:9.20E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.22|P-value:9.55E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.86|P-value:1.15E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.38|P-value:6.33E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.68|P-value:1.98E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.54|P-value:3.68E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.30|P-value:7.37E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.42|P-value:4.99E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.49|P-value:8.90E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.73|P-value:1.69E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.65|P-value:2.49E-4 ID:SGTC_2955|Compound:9076254|FD-Score:-4.47|P-value:8.74E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.34|P-value:7.09E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.49|P-value:9.17E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.90|P-value:8.74E-5 ID:SGTC_3335|Compound:9143315|FD-Score:3.79|P-value:1.51E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:3.49|P-value:4.37E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:3.90|P-value:9.67E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:7.59|P-value:1.94E-13 ID:SGTC_78|Compound:0336-0123|FD-Score:5.47|P-value:8.79E-8 ID:SGTC_144|Compound:4161-2749|FD-Score:4.05|P-value:5.46E-5 ID:SGTC_145|Compound:3737-0113|FD-Score:6.34|P-value:6.82E-10 ID:SGTC_184|Compound:1494-0393|FD-Score:3.70|P-value:2.05E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:3.25|P-value:9.64E-4 ID:SGTC_619|Compound:1652-1634|FD-Score:3.27|P-value:9.20E-4 ID:SGTC_633|Compound:0437-0093|FD-Score:-3.22|P-value:9.55E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:3.69|P-value:2.15E-4 ID:SGTC_656|Compound:4090-1979|FD-Score:3.86|P-value:1.15E-4 ID:SGTC_1241|Compound:0364-0022|FD-Score:3.38|P-value:6.33E-4 ID:SGTC_1521|Compound:st012942|FD-Score:-3.68|P-value:1.98E-4 ID:SGTC_1821|Compound:st053465|FD-Score:3.54|P-value:3.68E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.30|P-value:7.37E-4 ID:SGTC_2315|Compound:7733312|FD-Score:-3.42|P-value:4.99E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:4.49|P-value:8.90E-6 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.73|P-value:1.69E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:3.65|P-value:2.49E-4 ID:SGTC_2955|Compound:9076254|FD-Score:-4.47|P-value:8.74E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.34|P-value:7.09E-4 ID:SGTC_2975|Compound:9084847|FD-Score:4.49|P-value:9.17E-6 ID:SGTC_3330|Compound:9141424|FD-Score:-3.90|P-value:8.74E-5 ID:SGTC_3335|Compound:9143315|FD-Score:3.79|P-value:1.51E-4

Top fitness defect scores for YKR064W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_70 0141-0289 7.59 43.3 µM 1.94E-13 mitochondrial stress 45 1.60E-14
2 SGTC_145 3737-0113 6.34 38.4 µM 6.82E-10 DNA intercalators 24 1.16E-10
3 SGTC_78 0336-0123 5.47 156.8 µM 8.79E-8 8 2.31E-8
4 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 4.49 3.7 µM 8.90E-6 50 3.52E-6
5 SGTC_2975 9084847 4.49 53.7 µM 9.17E-6 15 3.63E-6
6 SGTC_144 4161-2749 4.05 112.4 µM 5.46E-5 DNA intercalators 23 2.53E-5
7 SGTC_9 0092-0002 3.90 10.4 µM 9.67E-5 28 4.72E-5
8 SGTC_656 4090-1979 3.86 187.0 µM 1.15E-4 21 5.68E-5
9 SGTC_3335 9143315 3.79 29.6 µM 1.51E-4 fatty acid desaturase (OLE1) 15 7.65E-5
10 SGTC_184 1494-0393 3.70 24.7 µM 2.05E-4 70 1.07E-4
11 SGTC_649 1123-0311 3.69 367.0 µM 2.15E-4 endomembrane recycling 81 1.12E-4
12 SGTC_2596 sinensetin 3.65 100.0 µM 2.49E-4 42 1.32E-4
13 SGTC_1821 st053465 3.54 65.6 µM 3.68E-4 14 2.02E-4
14 SGTC_6 0844-0013 3.49 19.8 µM 4.37E-4 141 2.43E-4
15 SGTC_1241 0364-0022 3.38 50.8 µM 6.33E-4 ERG2 50 3.64E-4
16 SGTC_2962 9082311 3.34 13.2 µM 7.09E-4 41 4.12E-4
17 SGTC_619 1652-1634 3.27 108.0 µM 9.20E-4 PDR1 44 5.46E-4
18 SGTC_324 1315-0088 3.25 19.6 µM 9.64E-4 RSC & ERG11 22 5.75E-4
19 SGTC_2762 leflunomide 3.22 42.1 µM 0.00107 59 6.41E-4
20 SGTC_1982 st072467 3.21 71.9 µM 0.00111 73 6.69E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 1.11E-32 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.198 8.74E-31 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.175 1.86E-24 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.166 3.46E-22 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.161 8.16E-21 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.154 2.98E-19 YMR030W RSF1 hom Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.153 7.10E-19 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.150 3.42E-18 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.145 2.88E-17 YBL029W_p YBL029W_p hom Non-essential protein of unknown function
0.144 7.28E-17 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.142 1.26E-16 YBR182C-A_p YBR182C-A_p hom Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
0.142 1.76E-16 YPR059C_d YPR059C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
0.141 3.15E-16 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.139 5.98E-16 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.138 9.24E-16 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain