YKR065C / PAM17

Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR065C / PAM17

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:3.64|P-value:2.29E-5 ID:SGTC_278|Compound:4092-0821|FD-Score:2.92|P-value:5.40E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.79|P-value:9.00E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-4.36|P-value:4.31E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.07|P-value:2.69E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:5.82|P-value:3.29E-11 ID:SGTC_556|Compound:4058-0063|FD-Score:-2.78|P-value:8.30E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.67|P-value:1.96E-5 ID:SGTC_651|Compound:3253-0966|FD-Score:-2.88|P-value:5.74E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-2.75|P-value:9.52E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.20|P-value:1.69E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.20|P-value:1.73E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:6.34|P-value:5.86E-13 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.48|P-value:4.87E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:2.95|P-value:4.86E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:-2.84|P-value:6.74E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.36|P-value:8.30E-5 ID:SGTC_1619|Compound:st003326|FD-Score:3.71|P-value:1.61E-5 ID:SGTC_1624|Compound:st003710|FD-Score:3.09|P-value:2.73E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.29|P-value:1.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.37|P-value:7.11E-5 ID:SGTC_2047|Compound:4141938|FD-Score:-3.29|P-value:1.04E-4 ID:SGTC_2143|Compound:5623677|FD-Score:4.16|P-value:1.56E-6 ID:SGTC_2201|Compound:6605497|FD-Score:3.99|P-value:3.90E-6 ID:SGTC_2313|Compound:7726885|FD-Score:3.44|P-value:5.85E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:2.78|P-value:9.17E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.24|P-value:1.03E-6 ID:SGTC_2435|Compound:5706476|FD-Score:5.06|P-value:7.03E-9 ID:SGTC_2557|Compound:derrustone|FD-Score:2.88|P-value:6.40E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.82|P-value:7.94E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.03|P-value:3.48E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-4.57|P-value:1.25E-7 ID:SGTC_2855|Compound:9016525|FD-Score:-4.92|P-value:1.45E-8 ID:SGTC_2915|Compound:7971052|FD-Score:2.84|P-value:7.46E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.36|P-value:8.36E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:3.64|P-value:2.29E-5 ID:SGTC_278|Compound:4092-0821|FD-Score:2.92|P-value:5.40E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:2.79|P-value:9.00E-4 ID:SGTC_359|Compound:0081-0058|FD-Score:-4.36|P-value:4.31E-7 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.07|P-value:2.69E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:5.82|P-value:3.29E-11 ID:SGTC_556|Compound:4058-0063|FD-Score:-2.78|P-value:8.30E-4 ID:SGTC_593|Compound:1203-0218|FD-Score:3.67|P-value:1.96E-5 ID:SGTC_651|Compound:3253-0966|FD-Score:-2.88|P-value:5.74E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:-2.75|P-value:9.52E-4 ID:SGTC_917|Compound:harmaline|FD-Score:3.20|P-value:1.69E-4 ID:SGTC_1079|Compound:idarubicin|FD-Score:3.20|P-value:1.73E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:6.34|P-value:5.86E-13 ID:SGTC_1288|Compound:0973-0013|FD-Score:3.48|P-value:4.87E-5 ID:SGTC_1306|Compound:1185-0270|FD-Score:2.95|P-value:4.86E-4 ID:SGTC_1384|Compound:3089-0840|FD-Score:-2.84|P-value:6.74E-4 ID:SGTC_1618|Compound:st003135|FD-Score:3.36|P-value:8.30E-5 ID:SGTC_1619|Compound:st003326|FD-Score:3.71|P-value:1.61E-5 ID:SGTC_1624|Compound:st003710|FD-Score:3.09|P-value:2.73E-4 ID:SGTC_1727|Compound:st034309|FD-Score:3.29|P-value:1.13E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.37|P-value:7.11E-5 ID:SGTC_2047|Compound:4141938|FD-Score:-3.29|P-value:1.04E-4 ID:SGTC_2143|Compound:5623677|FD-Score:4.16|P-value:1.56E-6 ID:SGTC_2201|Compound:6605497|FD-Score:3.99|P-value:3.90E-6 ID:SGTC_2313|Compound:7726885|FD-Score:3.44|P-value:5.85E-5 ID:SGTC_2407|Compound:paclitaxel|FD-Score:2.78|P-value:9.17E-4 ID:SGTC_2430|Compound:5571206|FD-Score:4.24|P-value:1.03E-6 ID:SGTC_2435|Compound:5706476|FD-Score:5.06|P-value:7.03E-9 ID:SGTC_2557|Compound:derrustone|FD-Score:2.88|P-value:6.40E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:2.82|P-value:7.94E-4 ID:SGTC_2829|Compound:7992743|FD-Score:3.03|P-value:3.48E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-4.57|P-value:1.25E-7 ID:SGTC_2855|Compound:9016525|FD-Score:-4.92|P-value:1.45E-8 ID:SGTC_2915|Compound:7971052|FD-Score:2.84|P-value:7.46E-4 ID:SGTC_3027|Compound:9089541|FD-Score:3.36|P-value:8.36E-5

Top fitness defect scores for YKR065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1083 didemethylchlorpromazine sulfoxide 6.34 707.4 nM 5.86E-13 redox potentiating 22 1.18E-10
2 SGTC_529 4076-0302 5.82 30.3 µM 3.29E-11 mitochondrial processes 3 2.92E-9
3 SGTC_2435 5706476 5.06 10.6 µM 7.03E-9 8 2.11E-7
4 SGTC_2430 5571206 4.24 200.0 µM 1.03E-6 48 1.13E-5
5 SGTC_2143 5623677 4.16 140.5 µM 1.56E-6 4 1.59E-5
6 SGTC_2201 6605497 3.99 169.9 µM 3.90E-6 ergosterol biosynthesis 8 3.31E-5
7 SGTC_1619 st003326 3.71 88.7 µM 1.61E-5 60S ribosome export 24 1.03E-4
8 SGTC_593 1203-0218 3.67 61.7 µM 1.96E-5 15 1.21E-4
9 SGTC_116 0155-0182 3.64 31.8 µM 2.29E-5 mitochondrial response to ROS 104 1.37E-4
10 SGTC_1288 0973-0013 3.48 65.5 µM 4.87E-5 28 2.51E-4
11 SGTC_2313 7726885 3.44 200.0 µM 5.85E-5 RPP1 & pyrimidine depletion 50 2.91E-4
12 SGTC_1618 st003135 3.36 44.5 µM 8.30E-5 33 3.86E-4
13 SGTC_3027 9089541 3.36 49.5 µM 8.36E-5 fatty acid desaturase (OLE1) 41 3.88E-4
14 SGTC_1727 st034309 3.29 9.9 µM 1.13E-4 78 4.94E-4
15 SGTC_917 harmaline 3.20 728.1 nM 1.69E-4 ergosterol biosynthesis 57 6.82E-4
16 SGTC_1079 idarubicin 3.20 2.4 µM 1.73E-4 DNA damage response 40 6.98E-4
17 SGTC_1624 st003710 3.09 6.2 µM 2.73E-4 calcium & mitochondrial duress 85 0.00101
18 SGTC_2829 7992743 3.03 58.4 µM 3.48E-4 88 0.00123
19 SGTC_1306 1185-0270 2.95 9.0 µM 4.86E-4 Golgi 90 0.00160
20 SGTC_278 4092-0821 2.92 23.9 µM 5.40E-4 plasma membrane duress 92 0.00174

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.289 1.05E-65 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.242 9.00E-46 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.216 8.44E-37 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.208 4.47E-34 YBR261C TAE1 hom AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
0.151 1.24E-18 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.127 1.86E-13 YFL015C_d YFL015C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
0.123 7.57E-13 YGL165C_d YGL165C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
0.117 9.90E-12 YLL012W YEH1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.112 8.82E-11 YDR096W GIS1 hom Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
0.111 1.14E-10 YDR506C GMC1 hom Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein
0.111 1.38E-10 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.109 2.20E-10 YKR023W_p YKR023W_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.106 8.87E-10 YPL196W OXR1 hom Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
0.104 1.76E-9 YFR018C_p YFR018C_p hom Putative protein of unknown function
0.103 2.43E-9 YFR028C CDC14 het Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis