HIPHOP chemogenomics database

YKR065C / PAM17

Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR065C / PAM17

Click on Significant Values for Screen Details

Top fitness defect scores for YKR065C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1083	didemethylchlorpromazine sulfoxide	6.34	707.4 nM	5.86E-13	redox potentiating	22	1.18E-10
2	SGTC_529	4076-0302	5.82	30.3 µM	3.29E-11	mitochondrial processes	3	2.92E-9
3	SGTC_2435	5706476	5.06	10.6 µM	7.03E-9		8	2.11E-7
4	SGTC_2430	5571206	4.24	200.0 µM	1.03E-6		48	1.13E-5
5	SGTC_2143	5623677	4.16	140.5 µM	1.56E-6		4	1.59E-5
6	SGTC_2201	6605497	3.99	169.9 µM	3.90E-6	ergosterol biosynthesis	8	3.31E-5
7	SGTC_1619	st003326	3.71	88.7 µM	1.61E-5	60S ribosome export	24	1.03E-4
8	SGTC_593	1203-0218	3.67	61.7 µM	1.96E-5		15	1.21E-4
9	SGTC_116	0155-0182	3.64	31.8 µM	2.29E-5	mitochondrial response to ROS	104	1.37E-4
10	SGTC_1288	0973-0013	3.48	65.5 µM	4.87E-5		28	2.51E-4
11	SGTC_2313	7726885	3.44	200.0 µM	5.85E-5	RPP1 & pyrimidine depletion	50	2.91E-4
12	SGTC_1618	st003135	3.36	44.5 µM	8.30E-5		33	3.86E-4
13	SGTC_3027	9089541	3.36	49.5 µM	8.36E-5	fatty acid desaturase (OLE1)	41	3.88E-4
14	SGTC_1727	st034309	3.29	9.9 µM	1.13E-4		78	4.94E-4
15	SGTC_917	harmaline	3.20	728.1 nM	1.69E-4	ergosterol biosynthesis	57	6.82E-4
16	SGTC_1079	idarubicin	3.20	2.4 µM	1.73E-4	DNA damage response	40	6.98E-4
17	SGTC_1624	st003710	3.09	6.2 µM	2.73E-4	calcium & mitochondrial duress	85	0.00101
18	SGTC_2829	7992743	3.03	58.4 µM	3.48E-4		88	0.00123
19	SGTC_1306	1185-0270	2.95	9.0 µM	4.86E-4	Golgi	90	0.00160
20	SGTC_278	4092-0821	2.92	23.9 µM	5.40E-4	plasma membrane duress	92	0.00174

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.289	1.05E-65	YPL251W_d	YPL251W_d	het	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.242	9.00E-46	YPL252C	YAH1	het	Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.216	8.44E-37	YDR431W_d	YDR431W_d	hom	Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.208	4.47E-34	YBR261C	TAE1	hom	AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
0.151	1.24E-18	YDR376W	ARH1	het	Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.127	1.86E-13	YFL015C_d	YFL015C_d	hom	Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
0.123	7.57E-13	YGL165C_d	YGL165C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
0.117	9.90E-12	YLL012W	YEH1	hom	Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.112	8.82E-11	YDR096W	GIS1	hom	Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
0.111	1.14E-10	YDR506C	GMC1	hom	Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein
0.111	1.38E-10	YDL049C	KNH1	hom	Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.109	2.20E-10	YKR023W_p	YKR023W_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.106	8.87E-10	YPL196W	OXR1	hom	Protein of unknown function required for normal levels of resistance to oxidative damage, null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
0.104	1.76E-9	YFR018C_p	YFR018C_p	hom	Putative protein of unknown function
0.103	2.43E-9	YFR028C	CDC14	het	Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR and Mitotic Exit Network in anaphase, enabling it to act on key substrates to effect a decrease in CDK/B-cyclin activity and mitotic exit; required for meiosis I spindle disassembly; released from nucleolus upon entry into anaphase I of meiosis, resequestered in metaphase II, then released again upon entry into anaphase II; maintained in nucleolus by Cdc55p in early meiosis