YKR076W / ECM4

Omega class glutathione transferase; not essential; similar to Ygr154cp; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR076W / ECM4

Click on Significant Values for Screen Details ID:SGTC_19|Compound:0279-0214|FD-Score:3.38|P-value:4.62E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:5.16|P-value:2.03E-7 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.64|P-value:1.65E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.05|P-value:3.44E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.25|P-value:7.21E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.67|P-value:1.58E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.75|P-value:1.53E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.26|P-value:1.43E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:3.42|P-value:4.00E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.20|P-value:8.31E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-3.92|P-value:5.54E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.34|P-value:4.92E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.46|P-value:3.46E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-4.09|P-value:2.82E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:-3.33|P-value:5.15E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.28|P-value:6.19E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.79|P-value:1.01E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.95|P-value:4.87E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.78|P-value:1.35E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.28|P-value:1.30E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.43|P-value:3.80E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.31|P-value:1.16E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.55|P-value:2.49E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.44|P-value:3.46E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.71|P-value:1.27E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.19|P-value:8.67E-4 ID:SGTC_1819|Compound:st053448|FD-Score:-3.49|P-value:2.93E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.49|P-value:2.91E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.68|P-value:1.51E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.54|P-value:2.52E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.60|P-value:2.01E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.29|P-value:6.12E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.87|P-value:7.36E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.26|P-value:6.85E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.45|P-value:3.30E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.22|P-value:7.83E-4 ID:SGTC_2237|Compound:6667747|FD-Score:3.17|P-value:9.27E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.38|P-value:4.23E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.25|P-value:7.01E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:6.56|P-value:5.59E-11 ID:SGTC_2827|Compound:7993847|FD-Score:3.29|P-value:6.17E-4 ID:SGTC_19|Compound:0279-0214|FD-Score:3.38|P-value:4.62E-4 ID:SGTC_20|Compound:0481-0013|FD-Score:5.16|P-value:2.03E-7 ID:SGTC_104|Compound:0074-0074|FD-Score:-3.64|P-value:1.65E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:4.05|P-value:3.44E-5 ID:SGTC_122|Compound:0236-0016|FD-Score:3.25|P-value:7.21E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.67|P-value:1.58E-4 ID:SGTC_263|Compound:alizarin|FD-Score:4.75|P-value:1.53E-6 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.26|P-value:1.43E-5 ID:SGTC_329|Compound:k048-0121|FD-Score:3.42|P-value:4.00E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.20|P-value:8.31E-4 ID:SGTC_626|Compound:0214-0009|FD-Score:-3.92|P-value:5.54E-5 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.34|P-value:4.92E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:3.46|P-value:3.46E-4 ID:SGTC_818|Compound:4072-2707|FD-Score:-4.09|P-value:2.82E-5 ID:SGTC_825|Compound:1988-2224|FD-Score:-3.33|P-value:5.15E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.28|P-value:6.19E-4 ID:SGTC_956|Compound:1185-0240|FD-Score:3.79|P-value:1.01E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:-3.95|P-value:4.87E-5 ID:SGTC_1066|Compound:ketoconazole|FD-Score:4.78|P-value:1.35E-6 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.28|P-value:1.30E-5 ID:SGTC_1158|Compound:4092-0494|FD-Score:3.43|P-value:3.80E-4 ID:SGTC_1177|Compound:0983-0204|FD-Score:4.31|P-value:1.16E-5 ID:SGTC_1235|Compound:benzylparaben|FD-Score:3.55|P-value:2.49E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:-3.44|P-value:3.46E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.71|P-value:1.27E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.19|P-value:8.67E-4 ID:SGTC_1819|Compound:st053448|FD-Score:-3.49|P-value:2.93E-4 ID:SGTC_1840|Compound:st055363|FD-Score:-3.49|P-value:2.91E-4 ID:SGTC_1891|Compound:st059073|FD-Score:3.68|P-value:1.51E-4 ID:SGTC_1907|Compound:st060210|FD-Score:3.54|P-value:2.52E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.60|P-value:2.01E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.29|P-value:6.12E-4 ID:SGTC_2050|Compound:5263056|FD-Score:3.87|P-value:7.36E-5 ID:SGTC_2054|Compound:5233173|FD-Score:3.26|P-value:6.85E-4 ID:SGTC_2059|Compound:5238311|FD-Score:-3.45|P-value:3.30E-4 ID:SGTC_2096|Compound:5468907|FD-Score:3.72|P-value:1.30E-4 ID:SGTC_2101|Compound:5377231|FD-Score:3.22|P-value:7.83E-4 ID:SGTC_2237|Compound:6667747|FD-Score:3.17|P-value:9.27E-4 ID:SGTC_2243|Compound:6634756|FD-Score:-3.38|P-value:4.23E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:3.25|P-value:7.01E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:6.56|P-value:5.59E-11 ID:SGTC_2827|Compound:7993847|FD-Score:3.29|P-value:6.17E-4

Top fitness defect scores for YKR076W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2682 pyrogallin 6.56 40.0 µM 5.59E-11 copper-dependent oxidative stress 2 2.62E-11
2 SGTC_20 0481-0013 5.16 4.9 µM 2.03E-7 10 1.25E-7
3 SGTC_1066 ketoconazole 4.78 628.0 nM 1.35E-6 9 8.89E-7
4 SGTC_263 alizarin 4.75 107.9 µM 1.53E-6 iron homeostasis 38 1.01E-6
5 SGTC_1177 0983-0204 4.31 23.6 µM 1.16E-5 11 8.18E-6
6 SGTC_1107 amlodipine 4.28 47.0 µM 1.30E-5 23 9.19E-6
7 SGTC_273 hydroxyurea 4.26 18.1 mM 1.43E-5 heme biosynthesis & mitochondrial translocase 28 1.02E-5
8 SGTC_116 0155-0182 4.05 31.8 µM 3.44E-5 mitochondrial response to ROS 72 2.52E-5
9 SGTC_2050 5263056 3.87 171.0 µM 7.36E-5 fatty acid desaturase (OLE1) 47 5.53E-5
10 SGTC_956 1185-0240 3.79 191.0 µM 1.01E-4 28 7.64E-5
11 SGTC_2096 5468907 3.72 200.0 µM 1.30E-4 13 9.92E-5
12 SGTC_1891 st059073 3.68 26.2 µM 1.51E-4 TSC3-RPN4 28 1.16E-4
13 SGTC_141 0443-0274 3.67 32.3 µM 1.58E-4 mitochondrial response to ROS 56 1.22E-4
14 SGTC_1920 st075728 3.60 45.7 µM 2.01E-4 34 1.56E-4
15 SGTC_1235 benzylparaben 3.55 21.5 µM 2.49E-4 19 1.95E-4
16 SGTC_1907 st060210 3.54 83.7 µM 2.52E-4 36 1.97E-4
17 SGTC_764 1951-2361 3.46 146.0 µM 3.46E-4 55 2.74E-4
18 SGTC_1158 4092-0494 3.43 91.8 µM 3.80E-4 30 3.02E-4
19 SGTC_329 k048-0121 3.42 21.3 µM 4.00E-4 RSC & ERG11 23 3.18E-4
20 SGTC_19 0279-0214 3.38 38.4 µM 4.62E-4 TRP & mitochondrial translation 66 3.69E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.161 7.59E-21 YDR032C PST2 hom Protein with similarity to a family of flavodoxin-like proteins; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
0.136 3.17E-15 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.101 5.16E-9 YLR188W MDL1 hom Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of peptides generated upon proteolysis of mitochondrial proteins, plays a role in the regulation of cellular resistance to oxidative stress
0.095 3.01E-8 YIR005W IST3 hom Component of the U2 snRNP, required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing
0.091 1.17E-7 YDR468C TLG1 het Essential t-SNARE that forms a complex with Tlg2p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
0.088 2.82E-7 YKL206C ADD66 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.087 4.08E-7 YOR209C NPT1 hom Nicotinate phosphoribosyltransferase, acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
0.084 1.01E-6 YNR026C SEC12 het Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
0.083 1.31E-6 YPL047W SGF11 hom Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
0.078 6.12E-6 YKR057W RPS21A hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
0.077 8.98E-6 YPL170W DAP1 hom Heme-binding protein involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
0.075 1.47E-5 YDL149W ATG9 hom Transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between the phagophore assembly site (PAS) and other cytosolic punctate structures, not found in autophagosomes; may be involved in membrane delivery to the PAS
0.074 1.61E-5 YLR214W FRE1 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels
0.073 2.11E-5 YCR092C MSH3 hom Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
0.073 2.61E-5 YKR098C UBP11 hom Ubiquitin-specific protease that cleaves ubiquitin from ubiquitinated proteins