YKR081C / RPF2

Essential protein involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR081C / RPF2

Click on Significant Values for Screen Details ID:SGTC_97|Compound:0535-0701|FD-Score:3.60|P-value:6.60E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:3.82|P-value:3.33E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.50|P-value:9.12E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:-3.84|P-value:2.91E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.93|P-value:2.33E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:5.43|P-value:6.26E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:-5.95|P-value:5.07E-8 ID:SGTC_1074|Compound:carmofur|FD-Score:6.04|P-value:3.55E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.46|P-value:3.53E-5 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.82|P-value:3.32E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.99|P-value:1.87E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.83|P-value:3.19E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.63|P-value:6.05E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.62|P-value:5.90E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.87|P-value:2.78E-4 ID:SGTC_1703|Compound:st026983|FD-Score:4.18|P-value:9.73E-5 ID:SGTC_1710|Compound:st031758|FD-Score:3.81|P-value:3.39E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.29|P-value:6.52E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.84|P-value:7.41E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.15|P-value:1.08E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.23|P-value:7.53E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.82|P-value:3.13E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:6.06|P-value:3.19E-8 ID:SGTC_2683|Compound:piperine|FD-Score:3.48|P-value:9.73E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.89|P-value:2.59E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.24|P-value:7.79E-5 ID:SGTC_2871|Compound:9038510|FD-Score:4.00|P-value:1.81E-4 ID:SGTC_2955|Compound:9076254|FD-Score:-6.77|P-value:6.77E-10 ID:SGTC_2961|Compound:9083290|FD-Score:3.75|P-value:4.18E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.60|P-value:6.60E-4 ID:SGTC_172|Compound:1112-0046|FD-Score:3.82|P-value:3.33E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:3.50|P-value:9.12E-4 ID:SGTC_790|Compound:1575-0012|FD-Score:-3.84|P-value:2.91E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.93|P-value:2.33E-4 ID:SGTC_843|Compound:0310-0051|FD-Score:5.43|P-value:6.26E-7 ID:SGTC_855|Compound:0428-0020|FD-Score:-5.95|P-value:5.07E-8 ID:SGTC_1074|Compound:carmofur|FD-Score:6.04|P-value:3.55E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.46|P-value:3.53E-5 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.82|P-value:3.32E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:3.99|P-value:1.87E-4 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.83|P-value:3.19E-4 ID:SGTC_1502|Compound:4534-4282|FD-Score:3.63|P-value:6.05E-4 ID:SGTC_1567|Compound:st069303|FD-Score:-3.62|P-value:5.90E-4 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:3.87|P-value:2.78E-4 ID:SGTC_1703|Compound:st026983|FD-Score:4.18|P-value:9.73E-5 ID:SGTC_1710|Compound:st031758|FD-Score:3.81|P-value:3.39E-4 ID:SGTC_2160|Compound:5660466|FD-Score:4.29|P-value:6.52E-5 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-4.84|P-value:7.41E-6 ID:SGTC_2568|Compound:diosmetin|FD-Score:4.15|P-value:1.08E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-4.23|P-value:7.53E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.82|P-value:3.13E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:6.06|P-value:3.19E-8 ID:SGTC_2683|Compound:piperine|FD-Score:3.48|P-value:9.73E-4 ID:SGTC_2804|Compound:7954240|FD-Score:3.89|P-value:2.59E-4 ID:SGTC_2838|Compound:9002687|FD-Score:4.24|P-value:7.79E-5 ID:SGTC_2871|Compound:9038510|FD-Score:4.00|P-value:1.81E-4 ID:SGTC_2955|Compound:9076254|FD-Score:-6.77|P-value:6.77E-10 ID:SGTC_2961|Compound:9083290|FD-Score:3.75|P-value:4.18E-4

Top fitness defect scores for YKR081C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2649 anisomycin 6.06 9.6 µM 3.19E-8 1 6.79E-10 1.23
2 SGTC_1074 carmofur 6.04 3.8 µM 3.55E-8 exosome 13 7.76E-10 0.06
3 SGTC_843 0310-0051 5.43 143.0 µM 6.26E-7 1 2.76E-8 0.88
4 SGTC_1130 3454-2858 4.46 15.9 µM 3.53E-5 NEO1 8 4.16E-6 0.30
5 SGTC_2160 5660466 4.29 132.9 µM 6.52E-5 60S ribosome export 13 8.91E-6 0.07
6 SGTC_2838 9002687 4.24 71.4 µM 7.79E-5 fatty acid desaturase (OLE1) 13 1.11E-5 0.33
7 SGTC_1703 st026983 4.18 48.3 µM 9.73E-5 1 1.47E-5 0.46
8 SGTC_2568 diosmetin 4.15 100.0 µM 1.08E-4 6 1.67E-5 0.15
9 SGTC_2871 9038510 4.00 13.0 µM 1.81E-4 3 3.17E-5 0.12
10 SGTC_1261 0712-0010 3.99 12.7 µM 1.87E-4 RNA processing & uracil transport 8 3.30E-5 0.04
11 SGTC_837 0091-0265 3.93 12.5 µM 2.33E-4 ubiquinone biosynthesis & proteasome 12 4.33E-5 0.03
12 SGTC_2804 7954240 3.89 19.5 µM 2.59E-4 12 4.93E-5 0.13
13 SGTC_1692 fluoroquinolonic acid 3.87 71.0 µM 2.78E-4 2 5.38E-5 0.09
14 SGTC_1492 4158-0558 3.83 28.5 µM 3.19E-4 60S ribosome export 2 6.39E-5 0.13
15 SGTC_1206 3978-0017 3.82 132.0 µM 3.32E-4 ERG2 7 6.70E-5 0.07
16 SGTC_172 1112-0046 3.82 2.6 µM 3.33E-4 NEO1 10 6.74E-5 0.00
17 SGTC_1710 st031758 3.81 61.5 µM 3.39E-4 4 6.88E-5 0.13
18 SGTC_2961 9083290 3.75 32.5 µM 4.18E-4 2 8.92E-5 0.28
19 SGTC_1502 4534-4282 3.63 27.7 µM 6.05E-4 3 1.41E-4 0.19
20 SGTC_97 0535-0701 3.60 19.1 µM 6.60E-4 60S ribosome export 13 1.57E-4 0.40

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.75E-14 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.117 1.27E-11 YNL228W_d YNL228W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1
0.116 1.86E-11 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.114 3.58E-11 YDR179C CSN9 hom Subunit of the Cop9 signalosome, which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
0.107 5.51E-10 YPR142C_d YPR142C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
0.096 2.73E-8 YIR007W_p YIR007W_p hom Putative glycosidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YIR007W is a non-essential gene
0.088 3.44E-7 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
0.086 6.35E-7 YDR011W SNQ2 hom Plasma membrane ATP-binding cassette (ABC) transporter, multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
0.084 1.06E-6 YHR170W NMD3 het Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
0.083 1.38E-6 YDR280W RRP45 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp45p (PM/SCL-75, EXOSC9); protein abundance increases in response to DNA replication stress
0.083 1.39E-6 YFR047C BNA6 hom Quinolinate phosphoribosyl transferase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
0.081 2.35E-6 YMR141C_d YMR141C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.080 3.18E-6 YGR036C CAX4 hom Dolichyl pyrophosphate (Dol-P-P) phosphatase with a luminally oriented active site in the ER, cleaves the anhydride linkage in Dol-P-P, required for Dol-P-P-linked oligosaccharide intermediate synthesis and protein N-glycosylation
0.080 3.20E-6 YLR278C_p YLR278C_p hom Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; YLR278C is not an essential gene
0.078 6.12E-6 YGL103W RPL28 het Ribosomal 60S subunit protein L28; homologous to mammalian ribosomal protein L27A and bacterial L15; may have peptidyl transferase activity; can mutate to cycloheximide resistance