Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
Zygosity: Heterozygous strain
fixedexpanded
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Top fitness defect scores for YKR083C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.195 | 5.10E-30 | YIL056W | VHR1 | hom | Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication |
0.152 | 8.06E-19 | YKL052C | ASK1 | het | Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress |
0.115 | 2.31E-11 | YPL011C | TAF3 | het | TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation |
0.112 | 9.27E-11 | YLR349W_d | YLR349W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C |
0.095 | 3.88E-8 | YDR416W | SYF1 | het | Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans |
0.092 | 8.34E-8 | YDR327W_d | YDR327W_d | het | Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1 |
0.091 | 1.38E-7 | YER148W | SPT15 | het | TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability |
0.088 | 3.15E-7 | YPR037C | ERV2 | hom | Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER |
0.088 | 3.63E-7 | YGL160W | AIM14 | hom | NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p |
0.085 | 8.03E-7 | YPL252C | YAH1 | het | Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin |
0.085 | 8.33E-7 | YKL053W_d | YKL053W_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1 |
0.084 | 1.08E-6 | YOR194C | TOA1 | het | TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA |
0.082 | 1.75E-6 | YGR216C | GPI1 | het | Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs |
0.082 | 2.20E-6 | YOR287C | RRP36 | het | Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit |
0.081 | 2.38E-6 | YJL039C | NUP192 | het | Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205 |