YKR083C / DAD2

Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR083C / DAD2

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:-3.60|P-value:3.00E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.72|P-value:2.94E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-7.38|P-value:1.47E-12 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.59|P-value:4.58E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:4.72|P-value:5.90E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:3.45|P-value:7.28E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.44|P-value:1.98E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.30|P-value:2.22E-5 ID:SGTC_456|Compound:gingerol|FD-Score:3.71|P-value:2.99E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:-3.33|P-value:7.54E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.83|P-value:3.79E-6 ID:SGTC_596|Compound:1155-0152|FD-Score:3.46|P-value:6.95E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.42|P-value:5.57E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:5.08|P-value:1.19E-6 ID:SGTC_661|Compound:1304-0007|FD-Score:4.11|P-value:6.98E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:5.27|P-value:4.92E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:4.18|P-value:5.36E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:3.55|P-value:5.26E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.51|P-value:5.97E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.48|P-value:6.59E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.08|P-value:7.87E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.44|P-value:1.93E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:6.85|P-value:8.88E-11 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.92|P-value:1.43E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.14|P-value:6.30E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:-5.87|P-value:1.37E-8 ID:SGTC_1273|Compound:0849-0096|FD-Score:6.25|P-value:3.05E-9 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.11|P-value:7.19E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.46|P-value:6.97E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.64|P-value:3.84E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:3.62|P-value:4.14E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:5.05|P-value:1.39E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:5.33|P-value:3.71E-7 ID:SGTC_1718|Compound:roemerine|FD-Score:3.83|P-value:1.99E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.71|P-value:2.99E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.41|P-value:5.77E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-7.01|P-value:1.67E-11 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.44|P-value:5.25E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-3.29|P-value:8.43E-4 ID:SGTC_1959|Compound:st077091|FD-Score:14.30|P-value:4.71E-41 ID:SGTC_2003|Compound:4023581|FD-Score:-3.68|P-value:2.25E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.20|P-value:4.97E-5 ID:SGTC_2150|Compound:5556613|FD-Score:3.61|P-value:4.20E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.62|P-value:2.80E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.49|P-value:1.57E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.32|P-value:7.59E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.03|P-value:9.44E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-5.96|P-value:8.36E-9 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:5.64E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.70|P-value:6.62E-6 ID:SGTC_2894|Compound:9048625|FD-Score:3.67|P-value:3.41E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-5.42|P-value:1.38E-7 ID:SGTC_2973|Compound:9094665|FD-Score:8.47|P-value:1.47E-15 ID:SGTC_2974|Compound:9094440|FD-Score:13.60|P-value:1.89E-37 ID:SGTC_3103|Compound:9120822|FD-Score:-3.60|P-value:3.03E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.43|P-value:7.71E-4 ID:SGTC_3237|Compound:9133362|FD-Score:3.44|P-value:7.55E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.91|P-value:1.51E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:-3.60|P-value:3.00E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.72|P-value:2.94E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-7.38|P-value:1.47E-12 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:3.59|P-value:4.58E-4 ID:SGTC_339|Compound:3091-4833|FD-Score:4.72|P-value:5.90E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:3.45|P-value:7.28E-4 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:4.44|P-value:1.98E-5 ID:SGTC_431|Compound:k089-0097|FD-Score:-4.30|P-value:2.22E-5 ID:SGTC_456|Compound:gingerol|FD-Score:3.71|P-value:2.99E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:-3.33|P-value:7.54E-4 ID:SGTC_576|Compound:1073-0113|FD-Score:4.83|P-value:3.79E-6 ID:SGTC_596|Compound:1155-0152|FD-Score:3.46|P-value:6.95E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-3.42|P-value:5.57E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:5.08|P-value:1.19E-6 ID:SGTC_661|Compound:1304-0007|FD-Score:4.11|P-value:6.98E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:5.27|P-value:4.92E-7 ID:SGTC_739|Compound:1486-1183|FD-Score:4.18|P-value:5.36E-5 ID:SGTC_805|Compound:3852-0218|FD-Score:3.55|P-value:5.26E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.51|P-value:5.97E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.48|P-value:6.59E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.08|P-value:7.87E-5 ID:SGTC_1043|Compound:0916-0009|FD-Score:4.44|P-value:1.93E-5 ID:SGTC_1049|Compound:3226-0134|FD-Score:6.85|P-value:8.88E-11 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.92|P-value:1.43E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:4.14|P-value:6.30E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:-5.87|P-value:1.37E-8 ID:SGTC_1273|Compound:0849-0096|FD-Score:6.25|P-value:3.05E-9 ID:SGTC_1282|Compound:0886-0010|FD-Score:4.11|P-value:7.19E-5 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.46|P-value:6.97E-4 ID:SGTC_1379|Compound:2144-0755|FD-Score:3.64|P-value:3.84E-4 ID:SGTC_1419|Compound:3993-2473|FD-Score:3.62|P-value:4.14E-4 ID:SGTC_1458|Compound:k015-0034|FD-Score:5.05|P-value:1.39E-6 ID:SGTC_1464|Compound:k208-0027|FD-Score:5.33|P-value:3.71E-7 ID:SGTC_1718|Compound:roemerine|FD-Score:3.83|P-value:1.99E-4 ID:SGTC_1742|Compound:st038235|FD-Score:3.71|P-value:2.99E-4 ID:SGTC_1815|Compound:st051655|FD-Score:-3.41|P-value:5.77E-4 ID:SGTC_1920|Compound:st075728|FD-Score:-7.01|P-value:1.67E-11 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:-3.44|P-value:5.25E-4 ID:SGTC_1934|Compound:st074720|FD-Score:-3.29|P-value:8.43E-4 ID:SGTC_1959|Compound:st077091|FD-Score:14.30|P-value:4.71E-41 ID:SGTC_2003|Compound:4023581|FD-Score:-3.68|P-value:2.25E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.20|P-value:4.97E-5 ID:SGTC_2150|Compound:5556613|FD-Score:3.61|P-value:4.20E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-3.62|P-value:2.80E-4 ID:SGTC_2341|Compound:7987486|FD-Score:4.49|P-value:1.57E-5 ID:SGTC_2414|Compound:st077232|FD-Score:-3.32|P-value:7.59E-4 ID:SGTC_2479|Compound:5617353|FD-Score:4.03|P-value:9.44E-5 ID:SGTC_2526|Compound:patulin|FD-Score:-5.96|P-value:8.36E-9 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:5.64E-5 ID:SGTC_2780|Compound:5215298|FD-Score:4.70|P-value:6.62E-6 ID:SGTC_2894|Compound:9048625|FD-Score:3.67|P-value:3.41E-4 ID:SGTC_2971|Compound:9092186|FD-Score:-5.42|P-value:1.38E-7 ID:SGTC_2973|Compound:9094665|FD-Score:8.47|P-value:1.47E-15 ID:SGTC_2974|Compound:9094440|FD-Score:13.60|P-value:1.89E-37 ID:SGTC_3103|Compound:9120822|FD-Score:-3.60|P-value:3.03E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.43|P-value:7.71E-4 ID:SGTC_3237|Compound:9133362|FD-Score:3.44|P-value:7.55E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.91|P-value:1.51E-4

Top fitness defect scores for YKR083C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1959 st077091 14.30 55.8 µM 4.71E-41 RPP1 & pyrimidine depletion 2 1.12E-46 2.38
2 SGTC_2974 9094440 13.60 9.7 µM 1.89E-37 ergosterol biosynthesis 1 1.42E-42 2.31
3 SGTC_2973 9094665 8.47 18.0 µM 1.47E-15 NEO1 4 1.27E-17 1.61
4 SGTC_1049 3226-0134 6.85 66.0 µM 8.88E-11 2 3.61E-12 1.21
5 SGTC_1273 0849-0096 6.25 14.1 µM 3.05E-9 RPP1 & pyrimidine depletion 2 2.04E-10 0.03
6 SGTC_1464 k208-0027 5.33 110.0 µM 3.71E-7 2 4.91E-8 0.73
7 SGTC_666 0272-0154 5.27 136.0 µM 4.92E-7 cell wall 8 6.78E-8 0.47
8 SGTC_642 0335-0876 5.08 158.0 µM 1.19E-6 ubiquinone biosynthesis & proteasome 7 1.86E-7 0.41
9 SGTC_1458 k015-0034 5.05 6.3 µM 1.39E-6 2 2.21E-7 1.31
10 SGTC_576 1073-0113 4.83 22.7 µM 3.79E-6 redox potentiating 29 6.98E-7 0.02
11 SGTC_339 3091-4833 4.72 71.4 µM 5.90E-6 iron homeostasis 6 1.16E-6 0.33
12 SGTC_2780 5215298 4.70 71.4 µM 6.62E-6 11 1.32E-6 0.29
13 SGTC_2341 7987486 4.49 176.7 µM 1.57E-5 3 3.52E-6 0.18
14 SGTC_1043 0916-0009 4.44 19.3 µM 1.93E-5 5 4.47E-6 0.40
15 SGTC_390 dec-rvkr-cmk 4.44 16.7 µM 1.98E-5 calcium & mitochondrial duress 2 4.60E-6 0.06
16 SGTC_2144 5628481 4.20 200.0 µM 4.97E-5 ERG2 2 1.32E-5 0.63
17 SGTC_739 1486-1183 4.18 203.0 µM 5.36E-5 2 1.44E-5 0.51
18 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 4.17 63.6 µM 5.64E-5 8 1.52E-5 0.00
19 SGTC_1129 3453-1384 4.14 223.0 µM 6.30E-5 14 1.73E-5 0.11
20 SGTC_661 1304-0007 4.11 33.6 µM 6.98E-5 3 1.94E-5 0.22

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.195 5.10E-30 YIL056W VHR1 hom Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
0.152 8.06E-19 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.115 2.31E-11 YPL011C TAF3 het TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.112 9.27E-11 YLR349W_d YLR349W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
0.095 3.88E-8 YDR416W SYF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.092 8.34E-8 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.091 1.38E-7 YER148W SPT15 het TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
0.088 3.15E-7 YPR037C ERV2 hom Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER
0.088 3.63E-7 YGL160W AIM14 hom NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
0.085 8.03E-7 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.085 8.33E-7 YKL053W_d YKL053W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
0.084 1.08E-6 YOR194C TOA1 het TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
0.082 1.75E-6 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.082 2.20E-6 YOR287C RRP36 het Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
0.081 2.38E-6 YJL039C NUP192 het Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205