HIPHOP chemogenomics database

YKR083C / DAD2

Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis

Zygosity: Heterozygous strain

fixedexpanded
Profile for YKR083C / DAD2

Click on Significant Values for Screen Details

Top fitness defect scores for YKR083C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_1959	st077091	14.30	55.8 µM	4.71E-41	RPP1 & pyrimidine depletion	2	1.12E-46	2.38
2	SGTC_2974	9094440	13.60	9.7 µM	1.89E-37	ergosterol biosynthesis	1	1.42E-42	2.31
3	SGTC_2973	9094665	8.47	18.0 µM	1.47E-15	NEO1	4	1.27E-17	1.61
4	SGTC_1049	3226-0134	6.85	66.0 µM	8.88E-11		2	3.61E-12	1.21
5	SGTC_1273	0849-0096	6.25	14.1 µM	3.05E-9	RPP1 & pyrimidine depletion	2	2.04E-10	0.03
6	SGTC_1464	k208-0027	5.33	110.0 µM	3.71E-7		2	4.91E-8	0.73
7	SGTC_666	0272-0154	5.27	136.0 µM	4.92E-7	cell wall	8	6.78E-8	0.47
8	SGTC_642	0335-0876	5.08	158.0 µM	1.19E-6	ubiquinone biosynthesis & proteasome	7	1.86E-7	0.41
9	SGTC_1458	k015-0034	5.05	6.3 µM	1.39E-6		2	2.21E-7	1.31
10	SGTC_576	1073-0113	4.83	22.7 µM	3.79E-6	redox potentiating	29	6.98E-7	0.02
11	SGTC_339	3091-4833	4.72	71.4 µM	5.90E-6	iron homeostasis	6	1.16E-6	0.33
12	SGTC_2780	5215298	4.70	71.4 µM	6.62E-6		11	1.32E-6	0.29
13	SGTC_2341	7987486	4.49	176.7 µM	1.57E-5		3	3.52E-6	0.18
14	SGTC_1043	0916-0009	4.44	19.3 µM	1.93E-5		5	4.47E-6	0.40
15	SGTC_390	dec-rvkr-cmk	4.44	16.7 µM	1.98E-5	calcium & mitochondrial duress	2	4.60E-6	0.06
16	SGTC_2144	5628481	4.20	200.0 µM	4.97E-5	ERG2	2	1.32E-5	0.63
17	SGTC_739	1486-1183	4.18	203.0 µM	5.36E-5		2	1.44E-5	0.51
18	SGTC_2527	2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone	4.17	63.6 µM	5.64E-5		8	1.52E-5	0.00
19	SGTC_1129	3453-1384	4.14	223.0 µM	6.30E-5		14	1.73E-5	0.11
20	SGTC_661	1304-0007	4.11	33.6 µM	6.98E-5		3	1.94E-5	0.22

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.195	5.10E-30	YIL056W	VHR1	hom	Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
0.152	8.06E-19	YKL052C	ASK1	het	Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.115	2.31E-11	YPL011C	TAF3	het	TFIID subunit (47 kDa), involved in promoter binding and RNA polymerase II transcription initiation
0.112	9.27E-11	YLR349W_d	YLR349W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
0.095	3.88E-8	YDR416W	SYF1	het	Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; null mutant has splicing defect and arrests in G2/M; homologs in human and C. elegans
0.092	8.34E-8	YDR327W_d	YDR327W_d	het	Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1
0.091	1.38E-7	YER148W	SPT15	het	TATA-binding protein, general transcription factor that interacts with other factors to form the preinitiation complex at promoters, essential for viability
0.088	3.15E-7	YPR037C	ERV2	hom	Flavin-linked sulfhydryl oxidase localized to the endoplasmic reticulum lumen, involved in disulfide bond formation within the ER
0.088	3.63E-7	YGL160W	AIM14	hom	NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
0.085	8.03E-7	YPL252C	YAH1	het	Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.085	8.33E-7	YKL053W_d	YKL053W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
0.084	1.08E-6	YOR194C	TOA1	het	TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; homologous to largest and second largest subunits of human and Drosophila TFIIA
0.082	1.75E-6	YGR216C	GPI1	het	Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.082	2.20E-6	YOR287C	RRP36	het	Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
0.081	2.38E-6	YJL039C	NUP192	het	Essential subunit of the inner ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport; homologous to human NUP205