HIPHOP chemogenomics database

YKR084C / HBS1

GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled; genetically implicated in mRNA no-go decay

Zygosity: Homozygous strain

fixedexpanded
Profile for YKR084C / HBS1

Click on Significant Values for Screen Details

Top fitness defect scores for YKR084C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1663	st014140	7.35	55.4 µM	2.02E-13	60S ribosome export	2	9.78E-14
2	SGTC_2788	5282734	6.24	71.4 µM	3.87E-10		10	2.25E-10
3	SGTC_2313	7726885	5.94	200.0 µM	2.27E-9	RPP1 & pyrimidine depletion	6	1.38E-9
4	SGTC_886	0866-0150	5.92	153.0 µM	2.56E-9		1	1.56E-9
5	SGTC_2097	5376186	5.88	200.0 µM	3.38E-9		4	2.07E-9
6	SGTC_1406	3909-7906	5.39	11.9 µM	5.34E-8	60S ribosome export	2	3.51E-8
7	SGTC_1990	st072050	4.92	59.8 µM	6.31E-7		4	4.41E-7
8	SGTC_2715	st079500	4.85	56.4 µM	8.62E-7		1	6.08E-7
9	SGTC_1670	st016595	4.69	4.0 µM	1.90E-6		3	1.37E-6
10	SGTC_2524	avocatin b	4.66	10.6 µM	2.18E-6		17	1.58E-6
11	SGTC_1403	3909-7815	4.41	62.9 µM	7.02E-6		11	5.22E-6
12	SGTC_2907	7721985	4.40	69.9 µM	7.34E-6		13	5.46E-6
13	SGTC_1428	4049-0204	4.37	61.7 µM	8.36E-6		6	6.24E-6
14	SGTC_979	1426-2828	4.28	121.0 µM	1.23E-5		16	9.23E-6
15	SGTC_789	1309-1135	4.26	70.6 µM	1.35E-5	ERG2	22	1.02E-5
16	SGTC_1805	st050013	4.25	60.3 µM	1.43E-5		3	1.08E-5
17	SGTC_1393	3470-5649	4.21	58.2 µM	1.69E-5	60S ribosome export	12	1.28E-5
18	SGTC_91	0417-1751	4.20	58.4 µM	1.73E-5	Golgi	44	1.32E-5
19	SGTC_1655	st012546	4.14	55.1 µM	2.23E-5		26	1.71E-5
20	SGTC_1429	4049-0205	4.14	9.5 µM	2.25E-5		10	1.72E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.113	4.91E-11	YHR195W	NVJ1	hom	Nuclear envelope protein, anchored to the nuclear inner membrane, that interacts with the vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN)
0.090	1.76E-7	YAR035W	YAT1	hom	Outer mitochondrial carnitine acetyltransferase, minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
0.087	4.45E-7	YGL141W	HUL5	hom	Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD
0.083	1.33E-6	YLR253W_p	YLR253W_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; transcription is periodic during the metabolic cycle; YLR253W is not an essential gene
0.081	2.30E-6	YML082W_p	YML082W_p	hom	Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; YML082W is not an essential gene
0.079	4.52E-6	YJL218W_p	YJL218W_p	hom	Putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in an OAF1/PIP2-dependent manner; promoter contains an oleate response element consensus sequence; non-essential gene
0.077	8.26E-6	YBR269C_p	FMP21_p	hom	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.077	8.70E-6	YPL177C	CUP9	hom	Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.073	2.60E-5	YBR024W	SCO2	hom	Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
0.069	5.99E-5	YPR130C_d	YPR130C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.067	1.06E-4	YOR385W_p	YOR385W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
0.066	1.18E-4	YDL081C	RPP1A	hom	Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.065	1.64E-4	YLR175W	CBF5	het	Pseudouridine synthase catalytic subunit of box H/ACA small nucleolar ribonucleoprotein particles (snoRNPs), acts on both large and small rRNAs and on snRNA U2; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita
0.065	1.73E-4	YPR009W	SUT2	hom	Putative transcription factor; multicopy suppressor of mutations that cause low activity of the cAMP/protein kinase A pathway; highly similar to Sut1p
0.065	1.76E-4	YOR291W	YPK9	hom	Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-type ATPase Spf1p; homolog of human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome