YKR086W / PRP16

DEAH-box RNA helicase involved in second catalytic step of splicing; exhibits ATP-dependent RNA unwinding activity; mediates the release of Yju2p and Cwc25p in the second step; in the absence of ATP, stabilizes the binding of Cwc25p to the spliceosome in the first catalytic step

Zygosity: Heterozygous strain
fixedexpanded
Profile for YKR086W / PRP16

Click on Significant Values for Screen Details ID:SGTC_34|Compound:0469-0683|FD-Score:-3.12|P-value:5.98E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:3.29|P-value:2.42E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.05|P-value:6.14E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.04|P-value:6.40E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:5.20|P-value:2.03E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:3.73|P-value:3.94E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:3.37|P-value:1.77E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.72|P-value:4.13E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:4.43|P-value:1.42E-6 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.57|P-value:1.03E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.31|P-value:2.30E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.91|P-value:1.80E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.51|P-value:9.18E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.14|P-value:5.62E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-4.13|P-value:8.87E-6 ID:SGTC_1530|Compound:rhein|FD-Score:3.22|P-value:3.25E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.12|P-value:4.68E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.00|P-value:1.16E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.67|P-value:5.90E-7 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.87|P-value:2.11E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.40|P-value:2.11E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.25|P-value:2.84E-4 ID:SGTC_1726|Compound:st035556|FD-Score:2.99|P-value:7.65E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.54|P-value:8.74E-5 ID:SGTC_1846|Compound:st056244|FD-Score:3.27|P-value:2.62E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.83|P-value:2.59E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:4.03|P-value:9.91E-6 ID:SGTC_1969|Compound:st069479|FD-Score:3.24|P-value:3.02E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-3.28|P-value:3.29E-4 ID:SGTC_2149|Compound:5554786|FD-Score:-3.88|P-value:2.74E-5 ID:SGTC_2201|Compound:6605497|FD-Score:3.19|P-value:3.57E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.20|P-value:4.45E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.26|P-value:2.80E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.64|P-value:7.82E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.68|P-value:4.94E-5 ID:SGTC_2431|Compound:5490079|FD-Score:3.25|P-value:2.86E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.49|P-value:1.09E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.44|P-value:1.36E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.47|P-value:1.20E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.59|P-value:6.31E-7 ID:SGTC_3327|Compound:9140648|FD-Score:3.36|P-value:1.88E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:-3.12|P-value:5.98E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:3.29|P-value:2.42E-4 ID:SGTC_219|Compound:mitoxanthrone|FD-Score:3.05|P-value:6.14E-4 ID:SGTC_296|Compound:1222-0039|FD-Score:3.04|P-value:6.40E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.42|P-value:1.44E-4 ID:SGTC_520|Compound:3584-0023|FD-Score:5.20|P-value:2.03E-8 ID:SGTC_522|Compound:1273-0010|FD-Score:3.73|P-value:3.94E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:3.37|P-value:1.77E-4 ID:SGTC_631|Compound:k072-0230|FD-Score:3.72|P-value:4.13E-5 ID:SGTC_817|Compound:1391-0715|FD-Score:4.43|P-value:1.42E-6 ID:SGTC_1184|Compound:1269-1718|FD-Score:-3.57|P-value:1.03E-4 ID:SGTC_1188|Compound:1403-0014|FD-Score:3.31|P-value:2.30E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:3.91|P-value:1.80E-5 ID:SGTC_1446|Compound:4239-0190|FD-Score:4.51|P-value:9.18E-7 ID:SGTC_1457|Compound:k015-0032|FD-Score:-3.14|P-value:5.62E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-4.13|P-value:8.87E-6 ID:SGTC_1530|Compound:rhein|FD-Score:3.22|P-value:3.25E-4 ID:SGTC_1533|Compound:dihydroergotamine mesylate|FD-Score:3.12|P-value:4.68E-4 ID:SGTC_1541|Compound:2',3',6-trimethoxyflavone|FD-Score:4.00|P-value:1.16E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:-4.67|P-value:5.90E-7 ID:SGTC_1574|Compound:spiramycin|FD-Score:3.87|P-value:2.11E-5 ID:SGTC_1681|Compound:tripelennamine|FD-Score:-3.40|P-value:2.11E-4 ID:SGTC_1691|Compound:st019933|FD-Score:3.25|P-value:2.84E-4 ID:SGTC_1726|Compound:st035556|FD-Score:2.99|P-value:7.65E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.54|P-value:8.74E-5 ID:SGTC_1846|Compound:st056244|FD-Score:3.27|P-value:2.62E-4 ID:SGTC_1886|Compound:st057356|FD-Score:3.83|P-value:2.59E-5 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:4.03|P-value:9.91E-6 ID:SGTC_1969|Compound:st069479|FD-Score:3.24|P-value:3.02E-4 ID:SGTC_2120|Compound:5538279|FD-Score:-3.28|P-value:3.29E-4 ID:SGTC_2149|Compound:5554786|FD-Score:-3.88|P-value:2.74E-5 ID:SGTC_2201|Compound:6605497|FD-Score:3.19|P-value:3.57E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.20|P-value:4.45E-4 ID:SGTC_2297|Compound:7971671|FD-Score:3.26|P-value:2.80E-4 ID:SGTC_2300|Compound:7976697|FD-Score:-3.64|P-value:7.82E-5 ID:SGTC_2360|Compound:9040895|FD-Score:3.68|P-value:4.94E-5 ID:SGTC_2431|Compound:5490079|FD-Score:3.25|P-value:2.86E-4 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.49|P-value:1.09E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:4.44|P-value:1.36E-6 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.47|P-value:1.20E-4 ID:SGTC_2760|Compound:etidronate|FD-Score:4.59|P-value:6.31E-7 ID:SGTC_3327|Compound:9140648|FD-Score:3.36|P-value:1.88E-4

Top fitness defect scores for YKR086W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_520 3584-0023 5.20 682.0 µM 2.03E-8 7 1.00E-7 0.39
2 SGTC_2760 etidronate 4.59 98.0 µM 6.31E-7 3 2.26E-6 0.13
3 SGTC_1446 4239-0190 4.51 62.0 µM 9.18E-7 4 3.17E-6 0.02
4 SGTC_2595 hexamethylquercetagetin 4.44 100.0 µM 1.36E-6 2 4.53E-6 0.07
5 SGTC_817 1391-0715 4.43 46.0 µM 1.42E-6 4 4.71E-6 0.21
6 SGTC_1894 3',6'-dihydroxyflavone 4.03 78.7 µM 9.91E-6 6 2.74E-5 0.60
7 SGTC_1541 2',3',6-trimethoxyflavone 4.00 64.0 µM 1.16E-5 4 3.16E-5 0.23
8 SGTC_1205 4161-2785 3.91 76.0 µM 1.80E-5 3 4.69E-5 0.16
9 SGTC_1574 spiramycin 3.87 23.7 µM 2.11E-5 5 5.43E-5 0.04
10 SGTC_1886 st057356 3.83 49.9 µM 2.59E-5 60S ribosome export 4 6.53E-5 0.16
11 SGTC_522 1273-0010 3.73 11.6 µM 3.94E-5 60S ribosome export 5 9.56E-5 0.01
12 SGTC_631 k072-0230 3.72 41.5 µM 4.13E-5 60S ribosome export 9 9.97E-5 0.10
13 SGTC_2360 9040895 3.68 200.0 µM 4.94E-5 RPP1 & pyrimidine depletion 11 1.17E-4 0.06
14 SGTC_1776 st049249 3.54 45.9 µM 8.74E-5 11 1.97E-4 0.05
15 SGTC_2499 avocadenofuran 3.49 51.5 µM 1.09E-4 5 2.41E-4 0.01
16 SGTC_2752 diclazuril 3.47 33.1 µM 1.20E-4 15 2.62E-4 0.13
17 SGTC_353 1988-1175 3.42 58.3 µM 1.44E-4 unfolded protein response 14 3.10E-4 0.04
18 SGTC_630 4356-0227 3.37 183.0 µM 1.77E-4 12 3.74E-4 0.02
19 SGTC_3327 9140648 3.36 26.9 µM 1.88E-4 26 3.95E-4 0.02
20 SGTC_1188 1403-0014 3.31 4.6 µM 2.30E-4 mitochondrial stress 19 4.73E-4 0.16

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.108 3.50E-10 YGR138C TPO2 hom Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
0.106 8.62E-10 YGR028W MSP1 hom Mitochondrial protein involved in sorting of proteins in the mitochondria; putative membrane-spanning ATPase
0.098 1.08E-8 YMR247C RKR1 hom RING domain E3 ubiquitin ligase; involved in the ubiquitin-mediated degradation of non-stop proteins; functional connections to chromatin modification; nuclear protein that also co-localizes with ribosomes; homolog of mouse Listerin, whose mutation has been reported to cause neurodegeneration in mice
0.090 1.83E-7 YER104W RTT105 het Protein with a role in regulation of Ty1 transposition
0.089 2.23E-7 YHR139C-A_d YHR139C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 2.10E-6 YBR065C ECM2 hom Pre-mRNA splicing factor, facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
0.075 1.33E-5 YPL147W PXA1 hom Subunit of a heterodimeric peroxisomal ATP-binding cassette transporter complex (Pxa1p-Pxa2p), required for import of long-chain fatty acids into peroxisomes; similarity to human adrenoleukodystrophy transporter ABCD1 and ABCD2 and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder
0.074 1.95E-5 YIR018C-A_p YIR018C-A_p hom Putative protein of unknown function; identified by expression profiling and mass spectrometry
0.072 2.69E-5 YHR129C ARP1 hom Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; putative ortholog of mammalian centractin
0.071 3.91E-5 YCR060W TAH1 hom Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); contains a single TPR domain with at least two TPR motifs; R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
0.069 6.48E-5 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.068 7.57E-5 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.068 8.92E-5 YBR175W SWD3 hom Essential subunit of the COMPASS (Set1C) complex, which methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
0.067 9.44E-5 YBR072W HSP26 hom Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
0.067 9.92E-5 YOR318C_d YOR318C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo