YKR097W / PCK1

Phosphoenolpyruvate carboxykinase, key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol

Zygosity: Homozygous strain
fixedexpanded
Profile for YKR097W / PCK1

Click on Significant Values for Screen Details ID:SGTC_86|Compound:0417-1630|FD-Score:3.52|P-value:1.98E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.27|P-value:8.39E-6 ID:SGTC_176|Compound:1486-1328|FD-Score:-4.13|P-value:1.59E-5 ID:SGTC_271|Compound:rotenone|FD-Score:5.20|P-value:8.09E-8 ID:SGTC_420|Compound:mitomycin C|FD-Score:4.71|P-value:1.03E-6 ID:SGTC_480|Compound:am 92016|FD-Score:3.73|P-value:8.55E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:6.16|P-value:2.72E-10 ID:SGTC_489|Compound:paxilline|FD-Score:3.51|P-value:2.08E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:4.84|P-value:5.51E-7 ID:SGTC_673|Compound:1082-0474|FD-Score:3.32|P-value:4.15E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:6.10|P-value:4.01E-10 ID:SGTC_760|Compound:0568-0725|FD-Score:4.36|P-value:5.53E-6 ID:SGTC_815|Compound:1582-0027|FD-Score:3.82|P-value:5.90E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.18|P-value:1.28E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.08|P-value:9.77E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.50|P-value:2.16E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.34|P-value:3.87E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.16|P-value:7.36E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.90|P-value:4.23E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.09|P-value:9.44E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.09|P-value:1.90E-5 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.61|P-value:1.75E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.50|P-value:2.16E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.28|P-value:4.71E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.41|P-value:2.93E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:4.50|P-value:2.93E-6 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.59|P-value:1.49E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.38|P-value:5.13E-6 ID:SGTC_1634|Compound:st006576|FD-Score:-4.16|P-value:1.40E-5 ID:SGTC_1835|Compound:st008666|FD-Score:4.60|P-value:1.81E-6 ID:SGTC_1867|Compound:st057372|FD-Score:3.29|P-value:4.64E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.19|P-value:6.54E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.24|P-value:5.49E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.51|P-value:2.81E-6 ID:SGTC_2017|Compound:5132927|FD-Score:4.91|P-value:3.88E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.33|P-value:4.07E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.39|P-value:3.24E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.17|P-value:7.19E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.29|P-value:4.63E-4 ID:SGTC_2299|Compound:7976368|FD-Score:3.91|P-value:4.19E-5 ID:SGTC_2320|Compound:7373003|FD-Score:3.30|P-value:4.40E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.21|P-value:1.13E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.51|P-value:1.98E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.36|P-value:3.61E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.11|P-value:8.83E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.20|P-value:8.02E-8 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.00|P-value:2.85E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.13|P-value:8.14E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-4.73|P-value:9.21E-7 ID:SGTC_2822|Compound:7994718|FD-Score:4.96|P-value:2.89E-7 ID:SGTC_2880|Compound:9042983|FD-Score:3.76|P-value:7.72E-5 ID:SGTC_2920|Compound:7982904|FD-Score:4.27|P-value:8.59E-6 ID:SGTC_2989|Compound:9073649|FD-Score:4.08|P-value:1.97E-5 ID:SGTC_2996|Compound:9066192|FD-Score:7.29|P-value:1.01E-13 ID:SGTC_2999|Compound:9069765|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_3039|Compound:9090270|FD-Score:8.18|P-value:8.69E-17 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:6.39E-4 ID:SGTC_3082|Compound:9117008|FD-Score:4.28|P-value:8.09E-6 ID:SGTC_3088|Compound:9117578|FD-Score:4.48|P-value:3.20E-6 ID:SGTC_3127|Compound:9126946|FD-Score:4.30|P-value:7.51E-6 ID:SGTC_3196|Compound:9111891|FD-Score:4.63|P-value:1.54E-6 ID:SGTC_3214|Compound:9129694|FD-Score:3.36|P-value:3.55E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.50|P-value:2.11E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:3.52|P-value:1.98E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.27|P-value:8.39E-6 ID:SGTC_176|Compound:1486-1328|FD-Score:-4.13|P-value:1.59E-5 ID:SGTC_271|Compound:rotenone|FD-Score:5.20|P-value:8.09E-8 ID:SGTC_420|Compound:mitomycin C|FD-Score:4.71|P-value:1.03E-6 ID:SGTC_480|Compound:am 92016|FD-Score:3.73|P-value:8.55E-5 ID:SGTC_481|Compound:bay k-8644|FD-Score:6.16|P-value:2.72E-10 ID:SGTC_489|Compound:paxilline|FD-Score:3.51|P-value:2.08E-4 ID:SGTC_529|Compound:4076-0302|FD-Score:4.84|P-value:5.51E-7 ID:SGTC_673|Compound:1082-0474|FD-Score:3.32|P-value:4.15E-4 ID:SGTC_754|Compound:3474-0146|FD-Score:6.10|P-value:4.01E-10 ID:SGTC_760|Compound:0568-0725|FD-Score:4.36|P-value:5.53E-6 ID:SGTC_815|Compound:1582-0027|FD-Score:3.82|P-value:5.90E-5 ID:SGTC_1027|Compound:k015-0025|FD-Score:4.18|P-value:1.28E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_1058|Compound:3448-5425|FD-Score:3.08|P-value:9.77E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:3.50|P-value:2.16E-4 ID:SGTC_1163|Compound:k060-0048|FD-Score:3.34|P-value:3.87E-4 ID:SGTC_1165|Compound:k072-0275|FD-Score:3.16|P-value:7.36E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:3.90|P-value:4.23E-5 ID:SGTC_1313|Compound:1220-0047|FD-Score:3.09|P-value:9.44E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:4.09|P-value:1.90E-5 ID:SGTC_1352|Compound:1496-0991|FD-Score:4.61|P-value:1.75E-6 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.50|P-value:2.16E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.28|P-value:4.71E-4 ID:SGTC_1484|Compound:3473-2379|FD-Score:3.41|P-value:2.93E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:4.50|P-value:2.93E-6 ID:SGTC_1576|Compound:erythromycin ethylsuccinate|FD-Score:3.59|P-value:1.49E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.38|P-value:5.13E-6 ID:SGTC_1634|Compound:st006576|FD-Score:-4.16|P-value:1.40E-5 ID:SGTC_1835|Compound:st008666|FD-Score:4.60|P-value:1.81E-6 ID:SGTC_1867|Compound:st057372|FD-Score:3.29|P-value:4.64E-4 ID:SGTC_1920|Compound:st075728|FD-Score:3.49|P-value:2.18E-4 ID:SGTC_1922|Compound:5578187|FD-Score:3.19|P-value:6.54E-4 ID:SGTC_1950|Compound:st077106|FD-Score:3.24|P-value:5.49E-4 ID:SGTC_1952|Compound:st077036|FD-Score:4.51|P-value:2.81E-6 ID:SGTC_2017|Compound:5132927|FD-Score:4.91|P-value:3.88E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:3.33|P-value:4.07E-4 ID:SGTC_2251|Compound:lansoprazole|FD-Score:3.39|P-value:3.24E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.17|P-value:7.19E-4 ID:SGTC_2286|Compound:7945697|FD-Score:3.29|P-value:4.63E-4 ID:SGTC_2299|Compound:7976368|FD-Score:3.91|P-value:4.19E-5 ID:SGTC_2320|Compound:7373003|FD-Score:3.30|P-value:4.40E-4 ID:SGTC_2376|Compound:9072922|FD-Score:4.21|P-value:1.13E-5 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:-3.51|P-value:1.98E-4 ID:SGTC_2549|Compound:cedrol|FD-Score:3.36|P-value:3.61E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.39|P-value:3.21E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.11|P-value:8.83E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.20|P-value:8.02E-8 ID:SGTC_2689|Compound:tacrolimus|FD-Score:4.00|P-value:2.85E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:3.13|P-value:8.14E-4 ID:SGTC_2813|Compound:7971799|FD-Score:-4.73|P-value:9.21E-7 ID:SGTC_2822|Compound:7994718|FD-Score:4.96|P-value:2.89E-7 ID:SGTC_2880|Compound:9042983|FD-Score:3.76|P-value:7.72E-5 ID:SGTC_2920|Compound:7982904|FD-Score:4.27|P-value:8.59E-6 ID:SGTC_2989|Compound:9073649|FD-Score:4.08|P-value:1.97E-5 ID:SGTC_2996|Compound:9066192|FD-Score:7.29|P-value:1.01E-13 ID:SGTC_2999|Compound:9069765|FD-Score:3.57|P-value:1.60E-4 ID:SGTC_3039|Compound:9090270|FD-Score:8.18|P-value:8.69E-17 ID:SGTC_3042|Compound:9091578|FD-Score:3.20|P-value:6.39E-4 ID:SGTC_3082|Compound:9117008|FD-Score:4.28|P-value:8.09E-6 ID:SGTC_3088|Compound:9117578|FD-Score:4.48|P-value:3.20E-6 ID:SGTC_3127|Compound:9126946|FD-Score:4.30|P-value:7.51E-6 ID:SGTC_3196|Compound:9111891|FD-Score:4.63|P-value:1.54E-6 ID:SGTC_3214|Compound:9129694|FD-Score:3.36|P-value:3.55E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.50|P-value:2.11E-4

Top fitness defect scores for YKR097W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3039 9090270 8.18 49.5 µM 8.69E-17 RPP1 & pyrimidine depletion 1 1.42E-16
2 SGTC_2996 9066192 7.29 71.4 µM 1.01E-13 5 1.50E-13
3 SGTC_481 bay k-8644 6.16 50.0 µM 2.72E-10 7 3.59E-10
4 SGTC_754 3474-0146 6.10 171.0 µM 4.01E-10 copper-dependent oxidative stress 5 5.27E-10
5 SGTC_2611 pyromycin 5.20 23.9 µM 8.02E-8 6 9.78E-8
6 SGTC_271 rotenone 5.20 740.0 µM 8.09E-8 8 9.87E-8
7 SGTC_2822 7994718 4.96 71.4 µM 2.89E-7 8 3.47E-7
8 SGTC_2017 5132927 4.91 60.6 µM 3.88E-7 11 4.63E-7
9 SGTC_529 4076-0302 4.84 30.3 µM 5.51E-7 mitochondrial processes 8 6.54E-7
10 SGTC_420 mitomycin C 4.71 81.9 mM 1.03E-6 10 1.21E-6
11 SGTC_3196 9111891 4.63 49.5 µM 1.54E-6 mitochondrial processes 9 1.81E-6
12 SGTC_1352 1496-0991 4.61 99.0 µM 1.75E-6 tubulin folding & SWR complex 18 2.05E-6
13 SGTC_1835 st008666 4.60 49.2 µM 1.81E-6 14 2.12E-6
14 SGTC_1952 st077036 4.51 64.1 µM 2.81E-6 19 3.27E-6
15 SGTC_1517 deoxycholic acid methyl ester 4.50 49.2 µM 2.93E-6 3 3.40E-6
16 SGTC_3088 9117578 4.48 49.5 µM 3.20E-6 amide catabolism 4 3.70E-6
17 SGTC_1629 st005085 4.38 44.9 µM 5.13E-6 RPP1 & pyrimidine depletion 23 5.91E-6
18 SGTC_760 0568-0725 4.36 82.9 µM 5.53E-6 5 6.36E-6
19 SGTC_3127 9126946 4.30 49.5 µM 7.51E-6 ERG2 13 8.61E-6
20 SGTC_3082 9117008 4.28 49.5 µM 8.09E-6 11 9.25E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.113 5.47E-11 YGL127C SOH1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
0.090 1.76E-7 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.079 4.39E-6 YKL106W AAT1 hom Mitochondrial aspartate aminotransferase, catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
0.075 1.25E-5 YPR187W RPO26 het RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; similar to bacterial omega subunit
0.073 2.17E-5 YNL122C_p YNL122C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL122C is not an essential gene
0.073 2.25E-5 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.072 3.08E-5 YOR156C NFI1 hom SUMO E3 ligase, catalyzes the covalent attachment of SUMO (Smt3p) to proteins; primary E3 ligase for Sir4p; sumoylates Yku70p/Yku80p and Sir4p in vivo to promote chromatin anchoring; promotes telomere anchoring to the nuclear envelope; involved in maintenance of proper telomere length
0.072 3.14E-5 YNL055C POR1 hom Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for the maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; phosphorylated; protein abundance increases in response to DNA replication stress; POR1 has a paralog, POR2, that arose from the whole genome duplication
0.069 6.32E-5 YPL036W PMA2 hom Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
0.069 6.73E-5 YLR339C_d YLR339C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
0.067 1.02E-4 YPR025C CCL1 het Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
0.066 1.44E-4 YCR051W_p YCR051W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
0.064 1.97E-4 YER167W BCK2 hom Protein rich in serine and threonine residues involved in protein kinase C signaling pathway, which controls cell integrity; overproduction suppresses pkc1 mutations
0.064 2.07E-4 YIR020W-A_d YIR020W-A_d hom Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data
0.064 2.26E-4 YOR180C DCI1 hom Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed