YLL013C / PUF3

Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL013C / PUF3

Click on Significant Values for Screen Details ID:SGTC_11|Compound:0438-0307|FD-Score:-3.25|P-value:3.30E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.75|P-value:5.25E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:6.61|P-value:3.58E-12 ID:SGTC_117|Compound:lucigenin|FD-Score:8.59|P-value:2.64E-19 ID:SGTC_126|Compound:0417-1624|FD-Score:4.24|P-value:5.69E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.79|P-value:3.65E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.77|P-value:4.12E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:3.09|P-value:7.19E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.48|P-value:1.63E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.50|P-value:1.49E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.94|P-value:6.34E-17 ID:SGTC_444|Compound:curcumin|FD-Score:-4.86|P-value:1.89E-7 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.31|P-value:4.07E-6 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.16|P-value:8.47E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.36|P-value:2.18E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.70|P-value:6.56E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.48|P-value:1.37E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.22|P-value:4.44E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:3.04|P-value:8.56E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.40|P-value:2.22E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.14|P-value:5.83E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.08|P-value:7.41E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:-3.27|P-value:3.09E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.56|P-value:1.18E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:4.63|P-value:8.34E-7 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.40|P-value:1.87E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.39|P-value:2.30E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.59|P-value:1.02E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.02|P-value:7.73E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.60|P-value:9.42E-7 ID:SGTC_1641|Compound:st009299|FD-Score:3.20|P-value:4.80E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.90|P-value:2.78E-5 ID:SGTC_1704|Compound:st028805|FD-Score:-3.11|P-value:5.59E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.88|P-value:2.21E-7 ID:SGTC_2033|Compound:5192550|FD-Score:5.18|P-value:3.97E-8 ID:SGTC_2135|Compound:5326629|FD-Score:3.29|P-value:3.43E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.07|P-value:6.48E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.04|P-value:8.54E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.98|P-value:1.92E-5 ID:SGTC_2534|Compound:helenine|FD-Score:3.01|P-value:9.56E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.87|P-value:3.15E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.21|P-value:4.58E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.48|P-value:1.36E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.11|P-value:6.62E-4 ID:SGTC_2945|Compound:9067681|FD-Score:3.31|P-value:3.12E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.34|P-value:2.79E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.73|P-value:4.75E-5 ID:SGTC_2988|Compound:9073640|FD-Score:13.80|P-value:9.08E-47 ID:SGTC_2991|Compound:9074669|FD-Score:-5.68|P-value:1.48E-9 ID:SGTC_2993|Compound:9054257|FD-Score:7.31|P-value:1.69E-14 ID:SGTC_2999|Compound:9069765|FD-Score:-5.44|P-value:6.79E-9 ID:SGTC_3037|Compound:9091026|FD-Score:4.54|P-value:1.28E-6 ID:SGTC_3044|Compound:9093193|FD-Score:-3.42|P-value:1.73E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.95|P-value:1.83E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.32|P-value:3.02E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.58|P-value:1.04E-6 ID:SGTC_3319|Compound:9138450|FD-Score:3.52|P-value:1.39E-4 ID:SGTC_11|Compound:0438-0307|FD-Score:-3.25|P-value:3.30E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:3.75|P-value:5.25E-5 ID:SGTC_68|Compound:0986-0284|FD-Score:6.61|P-value:3.58E-12 ID:SGTC_117|Compound:lucigenin|FD-Score:8.59|P-value:2.64E-19 ID:SGTC_126|Compound:0417-1624|FD-Score:4.24|P-value:5.69E-6 ID:SGTC_140|Compound:0062-0056|FD-Score:-3.79|P-value:3.65E-5 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.77|P-value:4.12E-5 ID:SGTC_364|Compound:0083-0097|FD-Score:3.09|P-value:7.19E-4 ID:SGTC_398|Compound:bronopol|FD-Score:3.48|P-value:1.63E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:3.50|P-value:1.49E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.94|P-value:6.34E-17 ID:SGTC_444|Compound:curcumin|FD-Score:-4.86|P-value:1.89E-7 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:4.31|P-value:4.07E-6 ID:SGTC_495|Compound:decylubiquinone|FD-Score:4.16|P-value:8.47E-6 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.36|P-value:2.18E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.70|P-value:6.56E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:-3.48|P-value:1.37E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.22|P-value:4.44E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:3.04|P-value:8.56E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.40|P-value:2.22E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.14|P-value:5.83E-4 ID:SGTC_1159|Compound:4092-0538|FD-Score:3.08|P-value:7.41E-4 ID:SGTC_1176|Compound:0848-0131|FD-Score:-3.27|P-value:3.09E-4 ID:SGTC_1182|Compound:1222-0449|FD-Score:4.56|P-value:1.18E-6 ID:SGTC_1185|Compound:1319-0113|FD-Score:4.63|P-value:8.34E-7 ID:SGTC_1255|Compound:0566-0045|FD-Score:-3.40|P-value:1.87E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.39|P-value:2.30E-4 ID:SGTC_1399|Compound:3562-5772|FD-Score:4.59|P-value:1.02E-6 ID:SGTC_1571|Compound:st070080|FD-Score:-3.02|P-value:7.73E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.60|P-value:9.42E-7 ID:SGTC_1641|Compound:st009299|FD-Score:3.20|P-value:4.80E-4 ID:SGTC_1674|Compound:st016616|FD-Score:3.90|P-value:2.78E-5 ID:SGTC_1704|Compound:st028805|FD-Score:-3.11|P-value:5.59E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.88|P-value:2.21E-7 ID:SGTC_2033|Compound:5192550|FD-Score:5.18|P-value:3.97E-8 ID:SGTC_2135|Compound:5326629|FD-Score:3.29|P-value:3.43E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.07|P-value:6.48E-4 ID:SGTC_2280|Compound:7785178|FD-Score:3.04|P-value:8.54E-4 ID:SGTC_2457|Compound:5731572|FD-Score:3.98|P-value:1.92E-5 ID:SGTC_2534|Compound:helenine|FD-Score:3.01|P-value:9.56E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.87|P-value:3.15E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:3.21|P-value:4.58E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.48|P-value:1.36E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.59|P-value:1.03E-4 ID:SGTC_2805|Compound:7950783|FD-Score:3.11|P-value:6.62E-4 ID:SGTC_2945|Compound:9067681|FD-Score:3.31|P-value:3.12E-4 ID:SGTC_2951|Compound:9072950|FD-Score:3.34|P-value:2.79E-4 ID:SGTC_2963|Compound:9082507|FD-Score:-3.73|P-value:4.75E-5 ID:SGTC_2988|Compound:9073640|FD-Score:13.80|P-value:9.08E-47 ID:SGTC_2991|Compound:9074669|FD-Score:-5.68|P-value:1.48E-9 ID:SGTC_2993|Compound:9054257|FD-Score:7.31|P-value:1.69E-14 ID:SGTC_2999|Compound:9069765|FD-Score:-5.44|P-value:6.79E-9 ID:SGTC_3037|Compound:9091026|FD-Score:4.54|P-value:1.28E-6 ID:SGTC_3044|Compound:9093193|FD-Score:-3.42|P-value:1.73E-4 ID:SGTC_3047|Compound:9092122|FD-Score:-3.95|P-value:1.83E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.32|P-value:3.02E-4 ID:SGTC_3207|Compound:9114163|FD-Score:4.58|P-value:1.04E-6 ID:SGTC_3319|Compound:9138450|FD-Score:3.52|P-value:1.39E-4

Top fitness defect scores for YLL013C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2988 9073640 13.80 71.4 µM 9.08E-47 1 1.58E-43
2 SGTC_117 lucigenin 8.59 22.1 µM 2.64E-19 redox potentiating 14 4.54E-18
3 SGTC_2993 9054257 7.31 71.4 µM 1.69E-14 fatty acid desaturase (OLE1) 12 1.31E-13
4 SGTC_68 0986-0284 6.61 82.8 µM 3.58E-12 14 1.89E-11
5 SGTC_2033 5192550 5.18 145.0 µM 3.97E-8 4 1.09E-7
6 SGTC_1746 st038117 4.88 42.0 µM 2.21E-7 16 5.38E-7
7 SGTC_1185 1319-0113 4.63 81.3 µM 8.34E-7 DNA damage response 5 1.85E-6
8 SGTC_1632 st005650 4.60 90.0 µM 9.42E-7 14 2.08E-6
9 SGTC_1399 3562-5772 4.59 25.0 µM 1.02E-6 RPP1 & pyrimidine depletion 8 2.25E-6
10 SGTC_3207 9114163 4.58 49.5 µM 1.04E-6 17 2.28E-6
11 SGTC_1182 1222-0449 4.56 220.0 µM 1.18E-6 9 2.55E-6
12 SGTC_3037 9091026 4.54 49.5 µM 1.28E-6 NEO1-PIK1 92 2.77E-6
13 SGTC_454 lyso-paf C-16 4.31 6.7 µM 4.07E-6 12 8.11E-6
14 SGTC_126 0417-1624 4.24 672.5 µM 5.69E-6 DNA intercalators 21 1.11E-5
15 SGTC_495 decylubiquinone 4.16 155.0 µM 8.47E-6 copper-dependent oxidative stress 19 1.61E-5
16 SGTC_2457 5731572 3.98 11.7 µM 1.92E-5 17 3.45E-5
17 SGTC_1674 st016616 3.90 23.6 µM 2.78E-5 28 4.88E-5
18 SGTC_2536 dehydroabietamide 3.87 32.0 µM 3.15E-5 39 5.48E-5
19 SGTC_58 0957-0393 3.75 9.0 µM 5.25E-5 cell wall 394 8.82E-5
20 SGTC_874 0599-0792 3.70 117.0 µM 6.56E-5 34 1.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.78E-18 YHR104W GRE3 hom Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by the HOG pathway; protein abundance increases in response to DNA replication stress
0.142 1.54E-16 YPR175W DPB2 het Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for maintenance of fidelity of chromosomal replication; expression peaks at the G1/S phase boundary; Cdc28p substrate
0.137 1.47E-15 YPR131C NAT3 hom Catalytic subunit of the NatB N-terminal acetyltransferase, which catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
0.124 4.52E-13 YPL099C AIM43 hom Protein of unknown function; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
0.121 1.82E-12 YEL003W GIM4 hom Subunit of the heterohexameric cochaperone prefoldin complex which binds specifically to cytosolic chaperonin and transfers target proteins to it
0.119 4.72E-12 YLR418C CDC73 hom Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; protein abundance increases in response to DNA replication stress
0.117 8.95E-12 YDL153C SAS10 het Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance
0.116 1.49E-11 YBR208C DUR1,2 hom Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress
0.108 3.73E-10 YHR181W SVP26 hom Integral membrane protein of the early Golgi apparatus and endoplasmic reticulum, involved in COP II vesicle transport; may also function to promote retention of proteins in the early Golgi compartment
0.107 4.95E-10 YGR155W CYS4 hom Cystathionine beta-synthase, catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog cause homocystinuria
0.101 4.08E-9 YLL027W ISA1 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins
0.101 4.93E-9 YKL170W MRPL38 hom Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
0.099 8.06E-9 YOR199W_d YOR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.095 3.67E-8 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
0.094 4.75E-8 YGL180W ATG1 hom Protein ser/thr kinase required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; during autophagy forms a complex with Atg13p and Atg17p