YLL019C / KNS1

Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL019C / KNS1

Click on Significant Values for Screen Details ID:SGTC_31|Compound:0508-1974|FD-Score:-3.20|P-value:4.36E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:4.45|P-value:8.96E-7 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.91|P-value:2.27E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:2.89|P-value:8.89E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.64|P-value:4.53E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.24|P-value:1.96E-8 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.16|P-value:5.10E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.05|P-value:7.68E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.93|P-value:7.68E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.04|P-value:5.16E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.25|P-value:2.28E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.54|P-value:7.06E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.44|P-value:1.69E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:-4.34|P-value:2.83E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:2.92|P-value:7.99E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.37|P-value:1.44E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:2.91|P-value:8.49E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.04|P-value:7.12E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.88|P-value:1.49E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:5.61|P-value:9.75E-10 ID:SGTC_1078|Compound:idebenone|FD-Score:-3.77|P-value:4.14E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.69|P-value:2.46E-7 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.42|P-value:1.17E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.36|P-value:1.49E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.01|P-value:3.93E-8 ID:SGTC_1308|Compound:1187-1617|FD-Score:-4.25|P-value:4.42E-6 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.37|P-value:1.41E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:4.89|P-value:8.14E-8 ID:SGTC_1529|Compound:apigenin|FD-Score:3.35|P-value:1.50E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.14|P-value:3.49E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.00|P-value:9.19E-4 ID:SGTC_1616|Compound:st002734|FD-Score:6.83|P-value:1.51E-13 ID:SGTC_1621|Compound:st003705|FD-Score:-3.12|P-value:5.86E-4 ID:SGTC_1623|Compound:st003709|FD-Score:-3.80|P-value:3.59E-5 ID:SGTC_1624|Compound:st003710|FD-Score:-4.12|P-value:8.38E-6 ID:SGTC_1691|Compound:st019933|FD-Score:2.98|P-value:6.42E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.12|P-value:5.86E-4 ID:SGTC_1841|Compound:st054148|FD-Score:2.95|P-value:7.35E-4 ID:SGTC_1882|Compound:5615643|FD-Score:2.87|P-value:9.78E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.39|P-value:1.26E-6 ID:SGTC_1995|Compound:st072814|FD-Score:-3.40|P-value:1.99E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-4.17|P-value:6.58E-6 ID:SGTC_2362|Compound:9071156|FD-Score:3.40|P-value:1.27E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.12|P-value:3.87E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.10|P-value:4.04E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.43|P-value:1.09E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.45|P-value:9.11E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-4.81|P-value:2.37E-7 ID:SGTC_2564|Compound:betulinic acid|FD-Score:8.15|P-value:1.82E-18 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.98|P-value:9.35E-8 ID:SGTC_2825|Compound:7998094|FD-Score:2.94|P-value:7.50E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.94|P-value:1.97E-5 ID:SGTC_3041|Compound:9091929|FD-Score:-3.59|P-value:9.07E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.99|P-value:8.97E-6 ID:SGTC_31|Compound:0508-1974|FD-Score:-3.20|P-value:4.36E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:4.45|P-value:8.96E-7 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.91|P-value:2.27E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:2.89|P-value:8.89E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.64|P-value:4.53E-5 ID:SGTC_142|Compound:0335-0849|FD-Score:-5.24|P-value:1.96E-8 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.16|P-value:5.10E-4 ID:SGTC_226|Compound:1,3-diallylurea|FD-Score:-3.05|P-value:7.68E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:2.93|P-value:7.68E-4 ID:SGTC_381|Compound:3461-2219|FD-Score:3.04|P-value:5.16E-4 ID:SGTC_393|Compound:acivicin|FD-Score:3.25|P-value:2.28E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.54|P-value:7.06E-5 ID:SGTC_566|Compound:0958-0068|FD-Score:-3.44|P-value:1.69E-4 ID:SGTC_579|Compound:4491-1009|FD-Score:-4.34|P-value:2.83E-6 ID:SGTC_676|Compound:falcarinol|FD-Score:2.92|P-value:7.99E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.37|P-value:1.44E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:2.91|P-value:8.49E-4 ID:SGTC_994|Compound:3910-0338|FD-Score:4.04|P-value:7.12E-6 ID:SGTC_1002|Compound:4049-0206|FD-Score:3.88|P-value:1.49E-5 ID:SGTC_1006|Compound:4099-6547|FD-Score:5.61|P-value:9.75E-10 ID:SGTC_1078|Compound:idebenone|FD-Score:-3.77|P-value:4.14E-5 ID:SGTC_1183|Compound:1254-0324|FD-Score:4.69|P-value:2.46E-7 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.42|P-value:1.17E-4 ID:SGTC_1284|Compound:0929-0043|FD-Score:3.36|P-value:1.49E-4 ID:SGTC_1296|Compound:1068-0102|FD-Score:5.01|P-value:3.93E-8 ID:SGTC_1308|Compound:1187-1617|FD-Score:-4.25|P-value:4.42E-6 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.37|P-value:1.41E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:4.89|P-value:8.14E-8 ID:SGTC_1529|Compound:apigenin|FD-Score:3.35|P-value:1.50E-4 ID:SGTC_1567|Compound:st069303|FD-Score:3.14|P-value:3.49E-4 ID:SGTC_1608|Compound:st001473|FD-Score:-3.00|P-value:9.19E-4 ID:SGTC_1616|Compound:st002734|FD-Score:6.83|P-value:1.51E-13 ID:SGTC_1621|Compound:st003705|FD-Score:-3.12|P-value:5.86E-4 ID:SGTC_1623|Compound:st003709|FD-Score:-3.80|P-value:3.59E-5 ID:SGTC_1624|Compound:st003710|FD-Score:-4.12|P-value:8.38E-6 ID:SGTC_1691|Compound:st019933|FD-Score:2.98|P-value:6.42E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-3.12|P-value:5.86E-4 ID:SGTC_1841|Compound:st054148|FD-Score:2.95|P-value:7.35E-4 ID:SGTC_1882|Compound:5615643|FD-Score:2.87|P-value:9.78E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:4.39|P-value:1.26E-6 ID:SGTC_1995|Compound:st072814|FD-Score:-3.40|P-value:1.99E-4 ID:SGTC_2307|Compound:7538926|FD-Score:-4.17|P-value:6.58E-6 ID:SGTC_2362|Compound:9071156|FD-Score:3.40|P-value:1.27E-4 ID:SGTC_2363|Compound:9053361|FD-Score:3.12|P-value:3.87E-4 ID:SGTC_2420|Compound:aureobasidin a|FD-Score:3.10|P-value:4.04E-4 ID:SGTC_2466|Compound:5983409|FD-Score:3.43|P-value:1.09E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:4.45|P-value:9.11E-7 ID:SGTC_2552|Compound:juarezic acid|FD-Score:-4.81|P-value:2.37E-7 ID:SGTC_2564|Compound:betulinic acid|FD-Score:8.15|P-value:1.82E-18 ID:SGTC_2759|Compound:disulfiram|FD-Score:-4.98|P-value:9.35E-8 ID:SGTC_2825|Compound:7998094|FD-Score:2.94|P-value:7.50E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-3.94|P-value:1.97E-5 ID:SGTC_3041|Compound:9091929|FD-Score:-3.59|P-value:9.07E-5 ID:SGTC_3052|Compound:9092717|FD-Score:3.99|P-value:8.97E-6

Top fitness defect scores for YLL019C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2564 betulinic acid 8.15 100.0 µM 1.82E-18 2 1.90E-16
2 SGTC_1616 st002734 6.83 56.1 µM 1.51E-13 5 4.31E-12
3 SGTC_1006 4099-6547 5.61 255.0 µM 9.75E-10 8 1.02E-8
4 SGTC_1296 1068-0102 5.01 11.9 µM 3.93E-8 4 2.69E-7
5 SGTC_1494 4358-1428 4.89 177.0 µM 8.14E-8 9 5.13E-7
6 SGTC_1183 1254-0324 4.69 198.0 µM 2.46E-7 11 1.37E-6
7 SGTC_129 0088-0017 4.45 176.6 µM 8.96E-7 58 4.28E-6
8 SGTC_2536 dehydroabietamide 4.45 32.0 µM 9.11E-7 22 4.34E-6
9 SGTC_1888 azatryptophan 4.39 97.5 µM 1.26E-6 10 5.78E-6
10 SGTC_994 3910-0338 4.04 138.0 µM 7.12E-6 calcium & mitochondrial duress 45 2.68E-5
11 SGTC_3052 9092717 3.99 49.5 µM 8.97E-6 25 3.28E-5
12 SGTC_1002 4049-0206 3.88 172.0 µM 1.49E-5 35 5.14E-5
13 SGTC_141 0443-0274 3.64 32.3 µM 4.53E-5 mitochondrial response to ROS 58 1.37E-4
14 SGTC_453 enantio-paf c16 3.54 10.0 µM 7.06E-5 184 2.03E-4
15 SGTC_2466 5983409 3.43 37.1 µM 1.09E-4 34 2.99E-4
16 SGTC_1231 0242-0792 3.42 125.0 µM 1.17E-4 33 3.18E-4
17 SGTC_2362 9071156 3.40 126.0 µM 1.27E-4 25 3.42E-4
18 SGTC_1339 1486-1321 3.37 22.7 µM 1.41E-4 ERG2 46 3.75E-4
19 SGTC_899 1083-0020 3.37 478.0 µM 1.44E-4 41 3.81E-4
20 SGTC_1284 0929-0043 3.36 16.0 µM 1.49E-4 48 3.94E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.131 2.85E-14 YBR070C ALG14 het Component of UDP-GlcNAc transferase required for the second step of dolichyl-linked oligosaccharide synthesis; anchors the catalytic subunit Alg13p to the ER membrane; similar to bacterial and human glycosyltransferases
0.125 3.33E-13 YCR088W ABP1 hom Actin-binding protein of the cortical actin cytoskeleton, important for activation of the Arp2/3 complex that plays a key role actin in cytoskeleton organization; phosphorylation within its PRR (Proline-Rich Region), mediated by Cdc28p and Pho85p, protects Abp1p from proteolysis mediated by its own PEST sequences
0.102 3.63E-9 YGR087C PDC6 hom Minor isoform of pyruvate decarboxylase, decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
0.099 8.81E-9 YBL063W KIP1 hom Kinesin-related motor protein required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
0.098 1.52E-8 YLR310C CDC25 het Membrane bound guanine nucleotide exchange factor (GEF or GDP-release factor); indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1
0.096 2.64E-8 YJR096W YJR096W hom Putative xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.090 1.91E-7 YGL217C_d YGL217C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
0.089 2.45E-7 YJL137C GLG2 hom Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
0.088 2.83E-7 YGL221C NIF3 hom Protein of unknown function, similar to Listeria monocytogenes major sigma factor (rpoD gene product); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.088 3.01E-7 YNR049C MSO1 hom Probable component of the secretory vesicle docking complex; acts at a late step in secretion; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; relocalizes from bud neck to nucleus upon DNA replication stress
0.086 5.85E-7 YDR253C MET32 hom Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; MET32 has a paralog, MET31, that arose from the whole genome duplication
0.086 6.86E-7 YOR302W YOR302W hom CPA1 uORF, Arginine attenuator peptide, regulates translation of the CPA1 mRNA
0.085 8.98E-7 YIL010W DOT5 hom Nuclear thiol peroxidase which functions as an alkyl-hydroperoxide reductase during post-diauxic growth
0.083 1.35E-6 YMR302C YME2 hom Integral inner mitochondrial membrane protein with a role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
0.083 1.51E-6 YDR229W IVY1 hom Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase