YLL021W / SPA2

Component of the polarisome, which functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL021W / SPA2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.19|P-value:5.77E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:16.40|P-value:5.10E-51 ID:SGTC_206|Compound:4546-0025|FD-Score:3.48|P-value:6.56E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.49|P-value:2.33E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:5.94|P-value:2.57E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:3.35|P-value:9.94E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.19|P-value:5.64E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:7.69|P-value:9.95E-13 ID:SGTC_363|Compound:0455-0021|FD-Score:3.37|P-value:9.19E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.21|P-value:8.52E-7 ID:SGTC_710|Compound:3448-4187|FD-Score:3.83|P-value:2.06E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:4.10|P-value:7.93E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:3.84|P-value:1.99E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.73|P-value:4.37E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:5.44|P-value:3.00E-7 ID:SGTC_1220|Compound:1838-0075|FD-Score:6.99|P-value:7.84E-11 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.50|P-value:9.06E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.71|P-value:8.28E-8 ID:SGTC_1340|Compound:1486-1359|FD-Score:5.08|P-value:1.51E-6 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.54|P-value:1.48E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.49|P-value:6.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.00|P-value:1.13E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.12|P-value:7.40E-5 ID:SGTC_1793|Compound:st048256|FD-Score:3.51|P-value:5.97E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-4.87|P-value:6.40E-6 ID:SGTC_1940|Compound:st075990|FD-Score:-3.60|P-value:6.74E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.59|P-value:4.55E-4 ID:SGTC_2289|Compound:7946404|FD-Score:4.48|P-value:1.91E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.54|P-value:7.93E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-4.35|P-value:4.98E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-3.71|P-value:4.65E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.76|P-value:2.64E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.48|P-value:6.51E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.45|P-value:1.73E-9 ID:SGTC_2602|Compound:kawain|FD-Score:4.69|P-value:8.22E-6 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.71|P-value:4.69E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.39|P-value:2.70E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:5.04|P-value:1.82E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:6.61|P-value:7.00E-10 ID:SGTC_2662|Compound:daunorubicin|FD-Score:7.40|P-value:6.32E-12 ID:SGTC_2756|Compound:carbadox|FD-Score:4.95|P-value:2.67E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.77|P-value:2.53E-4 ID:SGTC_3304|Compound:9125678|FD-Score:6.38|P-value:2.51E-9 ID:SGTC_3311|Compound:9131146|FD-Score:4.92|P-value:3.03E-6 ID:SGTC_3321|Compound:9137793|FD-Score:4.06|P-value:9.33E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.83|P-value:2.07E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.19|P-value:5.77E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:16.40|P-value:5.10E-51 ID:SGTC_206|Compound:4546-0025|FD-Score:3.48|P-value:6.56E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:5.49|P-value:2.33E-7 ID:SGTC_304|Compound:1310-0298|FD-Score:5.94|P-value:2.57E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:3.35|P-value:9.94E-4 ID:SGTC_313|Compound:3474-0040|FD-Score:4.19|P-value:5.64E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:7.69|P-value:9.95E-13 ID:SGTC_363|Compound:0455-0021|FD-Score:3.37|P-value:9.19E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:5.21|P-value:8.52E-7 ID:SGTC_710|Compound:3448-4187|FD-Score:3.83|P-value:2.06E-4 ID:SGTC_801|Compound:0866-0028|FD-Score:4.10|P-value:7.93E-5 ID:SGTC_883|Compound:0850-0245|FD-Score:3.84|P-value:1.99E-4 ID:SGTC_906|Compound:1165-0455|FD-Score:-3.73|P-value:4.37E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:5.44|P-value:3.00E-7 ID:SGTC_1220|Compound:1838-0075|FD-Score:6.99|P-value:7.84E-11 ID:SGTC_1236|Compound:0330-0013|FD-Score:-3.50|P-value:9.06E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.71|P-value:8.28E-8 ID:SGTC_1340|Compound:1486-1359|FD-Score:5.08|P-value:1.51E-6 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.54|P-value:1.48E-5 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:3.49|P-value:6.35E-4 ID:SGTC_1655|Compound:st012546|FD-Score:4.00|P-value:1.13E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.12|P-value:7.40E-5 ID:SGTC_1793|Compound:st048256|FD-Score:3.51|P-value:5.97E-4 ID:SGTC_1922|Compound:5578187|FD-Score:-4.87|P-value:6.40E-6 ID:SGTC_1940|Compound:st075990|FD-Score:-3.60|P-value:6.74E-4 ID:SGTC_2210|Compound:7115885|FD-Score:3.59|P-value:4.55E-4 ID:SGTC_2289|Compound:7946404|FD-Score:4.48|P-value:1.91E-5 ID:SGTC_2337|Compound:9009472|FD-Score:-3.54|P-value:7.93E-4 ID:SGTC_2338|Compound:7999974|FD-Score:-4.35|P-value:4.98E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-3.71|P-value:4.65E-4 ID:SGTC_2463|Compound:5473429|FD-Score:3.76|P-value:2.64E-4 ID:SGTC_2564|Compound:betulinic acid|FD-Score:3.48|P-value:6.51E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:6.45|P-value:1.73E-9 ID:SGTC_2602|Compound:kawain|FD-Score:4.69|P-value:8.22E-6 ID:SGTC_2607|Compound:solasodine|FD-Score:-3.71|P-value:4.69E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:4.39|P-value:2.70E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:5.04|P-value:1.82E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:6.61|P-value:7.00E-10 ID:SGTC_2662|Compound:daunorubicin|FD-Score:7.40|P-value:6.32E-12 ID:SGTC_2756|Compound:carbadox|FD-Score:4.95|P-value:2.67E-6 ID:SGTC_2805|Compound:7950783|FD-Score:3.77|P-value:2.53E-4 ID:SGTC_3304|Compound:9125678|FD-Score:6.38|P-value:2.51E-9 ID:SGTC_3311|Compound:9131146|FD-Score:4.92|P-value:3.03E-6 ID:SGTC_3321|Compound:9137793|FD-Score:4.06|P-value:9.33E-5 ID:SGTC_3351|Compound:9150312|FD-Score:3.83|P-value:2.07E-4

Top fitness defect scores for YLL021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 16.40 40.9 µM 5.10E-51 cell wall 14 4.42E-61
2 SGTC_354 2783-3500 7.69 4.9 µM 9.95E-13 cell wall 11 7.23E-15
3 SGTC_2662 daunorubicin 7.40 22.4 µM 6.32E-12 anthracycline transcription coupled DNA repair 3 6.67E-14
4 SGTC_1220 1838-0075 6.99 193.0 µM 7.84E-11 2 1.37E-12
5 SGTC_2619 obliquin 6.61 87.9 µM 7.00E-10 cell wall 65 1.89E-11
6 SGTC_2584 androsterone 6.45 48.7 µM 1.73E-9 cell wall 49 5.59E-11
7 SGTC_3304 9125678 6.38 46.1 µM 2.51E-9 11 8.74E-11
8 SGTC_304 1310-0298 5.94 97.0 µM 2.57E-8 cell wall 21 1.42E-9
9 SGTC_1282 0886-0010 5.71 32.4 µM 8.28E-8 endomembrane recycling 11 5.74E-9
10 SGTC_221 trifluoperazine 5.49 10.3 µM 2.33E-7 NEO1-PIK1 144 1.98E-8
11 SGTC_916 4466-0038 5.44 42.0 nM 3.00E-7 cell wall 32 2.67E-8
12 SGTC_453 enantio-paf c16 5.21 10.0 µM 8.52E-7 80 9.31E-8
13 SGTC_1340 1486-1359 5.08 257.0 µM 1.51E-6 6 1.84E-7
14 SGTC_2612 dictamnine 5.04 15.5 µM 1.82E-6 RNA pol III & RNase P/MRP 23 2.30E-7
15 SGTC_2756 carbadox 4.95 75.1 µM 2.67E-6 35 3.63E-7
16 SGTC_3311 9131146 4.92 2.9 µM 3.03E-6 cell wall 87 4.22E-7
17 SGTC_2602 kawain 4.69 100.0 µM 8.22E-6 11 1.39E-6
18 SGTC_1456 k015-0012 4.54 13.0 µM 1.48E-5 21 2.79E-6
19 SGTC_2289 7946404 4.48 165.1 µM 1.91E-5 75 3.79E-6
20 SGTC_2608 thymoquinone 4.39 42.7 µM 2.70E-5 9 5.70E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.385 5.47E-119 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.366 4.88E-107 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.363 6.13E-105 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.360 2.30E-103 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.358 6.57E-102 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.355 5.32E-100 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.349 5.36E-97 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.341 7.65E-92 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.319 2.14E-80 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.317 4.50E-79 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.301 2.66E-71 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.296 6.39E-69 YDR351W SBE2 hom Protein involved in the transport of cell wall components from the Golgi to the cell surface; required for bud growth
0.293 2.78E-67 YJL073W JEM1 hom DnaJ-like chaperone required for nuclear membrane fusion during mating, localizes to the ER membrane; exhibits genetic interactions with KAR2
0.291 1.84E-66 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.291 2.28E-66 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions