YLL027W / ISA1

Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL027W / ISA1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:4.50|P-value:2.05E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.61|P-value:5.09E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.97|P-value:2.95E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:3.62|P-value:4.84E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.07|P-value:5.73E-11 ID:SGTC_118|Compound:0083-0097|FD-Score:3.82|P-value:2.49E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.28|P-value:5.16E-9 ID:SGTC_179|Compound:1273-0063|FD-Score:3.64|P-value:4.55E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:7.23|P-value:2.13E-11 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.71|P-value:3.58E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.84|P-value:5.11E-6 ID:SGTC_294|Compound:k038-0071|FD-Score:3.56|P-value:5.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.54|P-value:2.16E-7 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.96|P-value:1.49E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.72|P-value:3.54E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.97|P-value:1.63E-13 ID:SGTC_748|Compound:0136-0228|FD-Score:6.42|P-value:2.33E-9 ID:SGTC_767|Compound:0929-0023|FD-Score:3.72|P-value:3.52E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.42|P-value:9.14E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.58|P-value:1.47E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.68|P-value:4.05E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.58|P-value:5.58E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.68|P-value:3.80E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:6.76|P-value:3.61E-10 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.75|P-value:3.00E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.34|P-value:3.75E-5 ID:SGTC_1619|Compound:st003326|FD-Score:-3.65|P-value:4.20E-4 ID:SGTC_1682|Compound:st012968|FD-Score:4.13|P-value:8.23E-5 ID:SGTC_1715|Compound:st032160|FD-Score:-4.78|P-value:6.27E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.46|P-value:2.26E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.97|P-value:1.47E-4 ID:SGTC_1750|Compound:st081859|FD-Score:11.00|P-value:5.21E-24 ID:SGTC_1763|Compound:st044482|FD-Score:4.02|P-value:1.25E-4 ID:SGTC_1849|Compound:st056239|FD-Score:5.37|P-value:4.85E-7 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-3.43|P-value:8.53E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-5.86|P-value:4.22E-8 ID:SGTC_1887|Compound:st057648|FD-Score:3.76|P-value:3.11E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-6.79|P-value:2.67E-10 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:4.57|P-value:1.58E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.53|P-value:6.57E-4 ID:SGTC_2055|Compound:5235184|FD-Score:4.46|P-value:2.39E-5 ID:SGTC_2057|Compound:5236571|FD-Score:-3.82|P-value:2.39E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.14|P-value:8.07E-5 ID:SGTC_2126|Compound:5251174|FD-Score:-3.87|P-value:1.98E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.50|P-value:2.71E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.17|P-value:1.22E-6 ID:SGTC_2425|Compound:5628681|FD-Score:-3.38|P-value:9.93E-4 ID:SGTC_2469|Compound:5655019|FD-Score:-3.73|P-value:3.27E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.56|P-value:1.62E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.55|P-value:6.07E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.58|P-value:1.48E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.62|P-value:4.90E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-5.99|P-value:2.22E-8 ID:SGTC_2821|Compound:7996764|FD-Score:3.98|P-value:1.42E-4 ID:SGTC_2827|Compound:7993847|FD-Score:5.32|P-value:6.18E-7 ID:SGTC_2829|Compound:7992743|FD-Score:-4.42|P-value:2.68E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-7.58|P-value:2.10E-12 ID:SGTC_2941|Compound:9056145|FD-Score:-3.50|P-value:6.78E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.90|P-value:1.90E-4 ID:SGTC_3142|Compound:9086498|FD-Score:-4.53|P-value:1.72E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.87|P-value:2.08E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.47|P-value:7.89E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:4.50|P-value:2.05E-5 ID:SGTC_70|Compound:0141-0289|FD-Score:3.61|P-value:5.09E-4 ID:SGTC_86|Compound:0417-1630|FD-Score:4.97|P-value:2.95E-6 ID:SGTC_116|Compound:0155-0182|FD-Score:3.62|P-value:4.84E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:7.07|P-value:5.73E-11 ID:SGTC_118|Compound:0083-0097|FD-Score:3.82|P-value:2.49E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:6.28|P-value:5.16E-9 ID:SGTC_179|Compound:1273-0063|FD-Score:3.64|P-value:4.55E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:7.23|P-value:2.13E-11 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:3.71|P-value:3.58E-4 ID:SGTC_275|Compound:trichlorophene|FD-Score:4.84|P-value:5.11E-6 ID:SGTC_294|Compound:k038-0071|FD-Score:3.56|P-value:5.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.54|P-value:2.16E-7 ID:SGTC_339|Compound:3091-4833|FD-Score:-3.96|P-value:1.49E-4 ID:SGTC_347|Compound:4534-1311|FD-Score:3.72|P-value:3.54E-4 ID:SGTC_431|Compound:k089-0097|FD-Score:-7.97|P-value:1.63E-13 ID:SGTC_748|Compound:0136-0228|FD-Score:6.42|P-value:2.33E-9 ID:SGTC_767|Compound:0929-0023|FD-Score:3.72|P-value:3.52E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:3.42|P-value:9.14E-4 ID:SGTC_879|Compound:0831-1030|FD-Score:4.58|P-value:1.47E-5 ID:SGTC_996|Compound:3970-0799|FD-Score:3.68|P-value:4.05E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.58|P-value:5.58E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.68|P-value:3.80E-4 ID:SGTC_1260|Compound:0700-1617|FD-Score:6.76|P-value:3.61E-10 ID:SGTC_1310|Compound:1188-0297|FD-Score:-3.75|P-value:3.00E-4 ID:SGTC_1521|Compound:st012942|FD-Score:4.34|P-value:3.75E-5 ID:SGTC_1619|Compound:st003326|FD-Score:-3.65|P-value:4.20E-4 ID:SGTC_1682|Compound:st012968|FD-Score:4.13|P-value:8.23E-5 ID:SGTC_1715|Compound:st032160|FD-Score:-4.78|P-value:6.27E-6 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.46|P-value:2.26E-5 ID:SGTC_1740|Compound:st038286|FD-Score:3.97|P-value:1.47E-4 ID:SGTC_1750|Compound:st081859|FD-Score:11.00|P-value:5.21E-24 ID:SGTC_1763|Compound:st044482|FD-Score:4.02|P-value:1.25E-4 ID:SGTC_1849|Compound:st056239|FD-Score:5.37|P-value:4.85E-7 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-3.43|P-value:8.53E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-5.86|P-value:4.22E-8 ID:SGTC_1887|Compound:st057648|FD-Score:3.76|P-value:3.11E-4 ID:SGTC_1917|Compound:st066507|FD-Score:-6.79|P-value:2.67E-10 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:4.57|P-value:1.58E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.53|P-value:6.57E-4 ID:SGTC_2055|Compound:5235184|FD-Score:4.46|P-value:2.39E-5 ID:SGTC_2057|Compound:5236571|FD-Score:-3.82|P-value:2.39E-4 ID:SGTC_2086|Compound:5274770|FD-Score:4.14|P-value:8.07E-5 ID:SGTC_2126|Compound:5251174|FD-Score:-3.87|P-value:1.98E-4 ID:SGTC_2232|Compound:chlorophetanol|FD-Score:5.50|P-value:2.71E-7 ID:SGTC_2250|Compound:alverine citrate|FD-Score:5.17|P-value:1.22E-6 ID:SGTC_2425|Compound:5628681|FD-Score:-3.38|P-value:9.93E-4 ID:SGTC_2469|Compound:5655019|FD-Score:-3.73|P-value:3.27E-4 ID:SGTC_2488|Compound:5268135|FD-Score:4.56|P-value:1.62E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.55|P-value:6.07E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.58|P-value:1.48E-5 ID:SGTC_2812|Compound:7970281|FD-Score:3.62|P-value:4.90E-4 ID:SGTC_2819|Compound:7996655|FD-Score:-5.99|P-value:2.22E-8 ID:SGTC_2821|Compound:7996764|FD-Score:3.98|P-value:1.42E-4 ID:SGTC_2827|Compound:7993847|FD-Score:5.32|P-value:6.18E-7 ID:SGTC_2829|Compound:7992743|FD-Score:-4.42|P-value:2.68E-5 ID:SGTC_2896|Compound:9052656|FD-Score:-7.58|P-value:2.10E-12 ID:SGTC_2941|Compound:9056145|FD-Score:-3.50|P-value:6.78E-4 ID:SGTC_2975|Compound:9084847|FD-Score:3.90|P-value:1.90E-4 ID:SGTC_3142|Compound:9086498|FD-Score:-4.53|P-value:1.72E-5 ID:SGTC_3211|Compound:9128781|FD-Score:3.87|P-value:2.08E-4 ID:SGTC_3312|Compound:9133330|FD-Score:3.47|P-value:7.89E-4

Top fitness defect scores for YLL027W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1750 st081859 11.00 2.4 µM 5.21E-24 endomembrane recycling 36 1.87E-28
2 SGTC_236 1509-0011 7.23 5.3 µM 2.13E-11 265 2.40E-13
3 SGTC_117 lucigenin 7.07 22.1 µM 5.73E-11 redox potentiating 28 7.86E-13
4 SGTC_1260 0700-1617 6.76 7.8 µM 3.61E-10 calcium & mitochondrial duress 6 7.11E-12
5 SGTC_748 0136-0228 6.42 77.9 µM 2.33E-9 redox potentiating 32 6.62E-11
6 SGTC_173 1273-0061 6.28 2.7 µM 5.16E-9 mitochondrial stress 104 1.71E-10
7 SGTC_310 1273-0058 5.54 1.1 µM 2.16E-7 mitochondrial stress 159 1.48E-8
8 SGTC_2232 chlorophetanol 5.50 41.5 µM 2.71E-7 TRP & mitochondrial translation 55 1.95E-8
9 SGTC_1849 st056239 5.37 19.2 µM 4.85E-7 RPP1 & pyrimidine depletion 6 3.90E-8
10 SGTC_2827 7993847 5.32 71.4 µM 6.18E-7 8 5.21E-8
11 SGTC_2250 alverine citrate 5.17 130.0 µM 1.22E-6 88 1.17E-7
12 SGTC_86 0417-1630 4.97 471.9 µM 2.95E-6 ubiquinone biosynthesis & proteasome 115 3.36E-7
13 SGTC_275 trichlorophene 4.84 5.7 µM 5.11E-6 mitochondrial stress 179 6.49E-7
14 SGTC_879 0831-1030 4.58 162.0 µM 1.47E-5 30 2.29E-6
15 SGTC_2789 5281112 4.58 71.4 µM 1.48E-5 19 2.30E-6
16 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 4.57 70.1 µM 1.58E-5 12 2.49E-6
17 SGTC_2488 5268135 4.56 45.7 µM 1.62E-5 34 2.58E-6
18 SGTC_58 0957-0393 4.50 9.0 µM 2.05E-5 cell wall 328 3.40E-6
19 SGTC_2055 5235184 4.46 200.0 µM 2.39E-5 ERG2 18 4.08E-6
20 SGTC_1521 st012942 4.34 49.4 µM 3.75E-5 23 6.99E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.328 1.02E-84 YNL184C_p YNL184C_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.308 1.08E-74 YDR194C MSS116 hom DEAD-box protein required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing
0.303 3.12E-72 YPR067W ISA2 hom Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
0.300 1.18E-70 YPR100W MRPL51 hom Mitochondrial ribosomal protein of the large subunit
0.299 3.38E-70 YNL177C MRPL22 hom Mitochondrial ribosomal protein of the large subunit
0.298 7.25E-70 YDR175C RSM24 hom Mitochondrial ribosomal protein of the small subunit
0.297 1.60E-69 YDR296W MHR1 hom Protein involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
0.297 5.00E-69 YMR287C DSS1 hom 3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.297 3.38E-69 YDR405W MRP20 hom Mitochondrial ribosomal protein of the large subunit
0.292 4.69E-67 YPL040C ISM1 hom Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.292 1.06E-66 YMR293C HER2 hom Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
0.291 2.67E-66 YER050C RSM18 hom Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.290 6.45E-66 YMR193W MRPL24 hom Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.289 1.97E-65 YKL016C ATP7 hom Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.287 8.01E-65 YGR215W RSM27 hom Mitochondrial ribosomal protein of the small subunit