YLL035W / GRC3

Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLL035W / GRC3

Click on Significant Values for Screen Details ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.27|P-value:1.25E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-4.09|P-value:2.33E-6 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.09|P-value:2.70E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.56|P-value:3.55E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-4.02|P-value:3.30E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-4.01|P-value:3.64E-6 ID:SGTC_394|Compound:menadione|FD-Score:2.82|P-value:8.57E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.48|P-value:4.76E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.49|P-value:4.95E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:2.81|P-value:8.77E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.04|P-value:3.54E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.94|P-value:5.00E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.83|P-value:3.37E-11 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.14|P-value:2.18E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.33|P-value:6.87E-7 ID:SGTC_844|Compound:0330-0132|FD-Score:2.89|P-value:6.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.52|P-value:4.03E-5 ID:SGTC_1115|Compound:galangin|FD-Score:3.26|P-value:1.41E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-2.87|P-value:6.60E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.96|P-value:5.14E-6 ID:SGTC_1176|Compound:0848-0131|FD-Score:4.60|P-value:1.42E-7 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.99|P-value:4.37E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.67|P-value:2.13E-5 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.21|P-value:1.71E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.95|P-value:5.24E-6 ID:SGTC_2005|Compound:4024620|FD-Score:-2.78|P-value:9.24E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.13|P-value:2.44E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.56|P-value:3.59E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.54|P-value:3.90E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.02|P-value:3.76E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.81|P-value:8.14E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:2.87|P-value:6.90E-4 ID:SGTC_3305|Compound:9127027|FD-Score:3.49|P-value:4.91E-5 ID:SGTC_3308|Compound:9128332|FD-Score:2.79|P-value:9.35E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-3.27|P-value:1.25E-4 ID:SGTC_261|Compound:5-fluorouridine|FD-Score:-4.09|P-value:2.33E-6 ID:SGTC_282|Compound:k297-0036|FD-Score:-3.09|P-value:2.70E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:3.56|P-value:3.55E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:-4.02|P-value:3.30E-6 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-4.01|P-value:3.64E-6 ID:SGTC_394|Compound:menadione|FD-Score:2.82|P-value:8.57E-4 ID:SGTC_412|Compound:gemfibrozil|FD-Score:-3.48|P-value:4.76E-5 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:3.49|P-value:4.95E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:2.81|P-value:8.77E-4 ID:SGTC_559|Compound:1550-0661|FD-Score:3.04|P-value:3.54E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-3.94|P-value:5.00E-6 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.83|P-value:3.37E-11 ID:SGTC_796|Compound:1234-0020|FD-Score:-3.14|P-value:2.18E-4 ID:SGTC_808|Compound:0711-0032|FD-Score:4.33|P-value:6.87E-7 ID:SGTC_844|Compound:0330-0132|FD-Score:2.89|P-value:6.38E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.52|P-value:4.03E-5 ID:SGTC_1115|Compound:galangin|FD-Score:3.26|P-value:1.41E-4 ID:SGTC_1122|Compound:capsaicin|FD-Score:-2.87|P-value:6.60E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.96|P-value:5.14E-6 ID:SGTC_1176|Compound:0848-0131|FD-Score:4.60|P-value:1.42E-7 ID:SGTC_1261|Compound:0712-0010|FD-Score:2.99|P-value:4.37E-4 ID:SGTC_1446|Compound:4239-0190|FD-Score:3.67|P-value:2.13E-5 ID:SGTC_1450|Compound:4112-4260|FD-Score:3.21|P-value:1.71E-4 ID:SGTC_1895|Compound:st058434|FD-Score:3.95|P-value:5.24E-6 ID:SGTC_2005|Compound:4024620|FD-Score:-2.78|P-value:9.24E-4 ID:SGTC_2011|Compound:4012611|FD-Score:3.13|P-value:2.44E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.56|P-value:3.59E-5 ID:SGTC_2324|Compound:6149169|FD-Score:3.54|P-value:3.90E-5 ID:SGTC_2435|Compound:5706476|FD-Score:3.02|P-value:3.76E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.81|P-value:8.14E-4 ID:SGTC_3061|Compound:glucosamine|FD-Score:2.87|P-value:6.90E-4 ID:SGTC_3305|Compound:9127027|FD-Score:3.49|P-value:4.91E-5 ID:SGTC_3308|Compound:9128332|FD-Score:2.79|P-value:9.35E-4

Top fitness defect scores for YLL035W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1176 0848-0131 4.60 83.3 µM 1.42E-7 Golgi 1 2.08E-6 0.77
2 SGTC_808 0711-0032 4.33 135.0 µM 6.87E-7 7 7.44E-6 0.15
3 SGTC_1136 pergolide 3.96 1.1 µM 5.14E-6 16 3.80E-5 0.01
4 SGTC_1895 st058434 3.95 18.3 µM 5.24E-6 3 3.86E-5 0.07
5 SGTC_1446 4239-0190 3.67 62.0 µM 2.13E-5 9 1.20E-4 0.24
6 SGTC_353 1988-1175 3.56 58.3 µM 3.55E-5 unfolded protein response 10 1.82E-4 0.05
7 SGTC_2048 5246248 3.56 200.0 µM 3.59E-5 4 1.84E-4 0.26
8 SGTC_2324 6149169 3.54 200.0 µM 3.90E-5 8 1.97E-4 0.22
9 SGTC_3305 9127027 3.49 53.8 µM 4.91E-5 9 2.37E-4 0.24
10 SGTC_415 dibenzyl ketone 3.49 100.0 µM 4.95E-5 6 2.39E-4 0.06
11 SGTC_1115 galangin 3.26 695.7 nM 1.41E-4 RPP1 & pyrimidine depletion 7 5.61E-4 0.10
12 SGTC_1450 4112-4260 3.21 37.6 µM 1.71E-4 calcium & mitochondrial duress 12 6.56E-4 0.08
13 SGTC_2011 4012611 3.13 177.0 µM 2.44E-4 mitochondrial processes 16 8.75E-4 0.07
14 SGTC_559 1550-0661 3.04 158.0 µM 3.54E-4 8 0.00118 0.02
15 SGTC_2435 5706476 3.02 10.6 µM 3.76E-4 7 0.00124 0.03
16 SGTC_1261 0712-0010 2.99 12.7 µM 4.37E-4 RNA processing & uracil transport 21 0.00141 0.12
17 SGTC_844 0330-0132 2.89 15.3 µM 6.38E-4 11 0.00191 8.34E-4
18 SGTC_3061 glucosamine 2.87 200.0 µM 6.90E-4 13 0.00204 0.18
19 SGTC_394 menadione 2.82 8.3 µM 8.57E-4 superoxide 21 0.00244 0.05
20 SGTC_473 2-arachidonoylglycerol 2.81 10.0 µM 8.77E-4 24 0.00248 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.211 3.03E-35 YFR027W ECO1 het Acetyltransferase; required for establishment of sister chromatid cohesion; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to ds DNA breaks; has a C2H2-type zinc finger; mutations in human homolog ESCO2 cause Roberts syndrome; relative distribution to the nucleus increases upon DNA replication stress
0.204 9.69E-33 YLR259C HSP60 het Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
0.180 8.45E-26 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.174 2.45E-24 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.172 1.14E-23 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.171 1.60E-23 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.162 3.24E-21 YOL141W PPM2 hom AdoMet-dependent tRNA methyltransferase also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
0.157 4.92E-20 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.157 5.10E-20 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.155 1.59E-19 YHR003C YHR003C hom Protein of unknown function, localized to the mitochondrial outer membrane
0.154 2.79E-19 YKL122C SRP21 het Subunit of the signal recognition particle (SRP), which functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
0.152 6.68E-19 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.152 7.42E-19 YHL040C ARN1 hom ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
0.151 1.63E-18 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.146 2.18E-17 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels