YLL049W / LDB18

Component of the dynactin complex, which is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24

Zygosity: Homozygous strain
fixedexpanded
Profile for YLL049W / LDB18

Click on Significant Values for Screen Details ID:SGTC_117|Compound:lucigenin|FD-Score:2.90|P-value:9.47E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.03|P-value:8.55E-6 ID:SGTC_456|Compound:gingerol|FD-Score:-3.69|P-value:4.82E-5 ID:SGTC_521|Compound:r062-0005|FD-Score:2.90|P-value:9.46E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.50|P-value:9.32E-5 ID:SGTC_732|Compound:3970-1727|FD-Score:2.92|P-value:9.01E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:2.91|P-value:9.41E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.77|P-value:2.78E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.68|P-value:4.33E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.09|P-value:5.54E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.71|P-value:2.51E-7 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.11|P-value:5.29E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-4.17|P-value:5.45E-6 ID:SGTC_1426|Compound:4048-4480|FD-Score:-2.96|P-value:9.04E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:2.92|P-value:9.04E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.13|P-value:4.09E-4 ID:SGTC_1826|Compound:st055655|FD-Score:2.96|P-value:7.63E-4 ID:SGTC_1841|Compound:st054148|FD-Score:-3.00|P-value:7.97E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.22|P-value:2.88E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.08|P-value:4.98E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.48|P-value:9.98E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.45|P-value:1.13E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.23|P-value:2.81E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.35|P-value:1.72E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.61|P-value:5.85E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.54|P-value:7.97E-5 ID:SGTC_2528|Compound:xanthyletin|FD-Score:2.89|P-value:9.94E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.19|P-value:3.92E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.07|P-value:5.19E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.34|P-value:1.80E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.16|P-value:3.67E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.30|P-value:2.10E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.63|P-value:5.18E-5 ID:SGTC_2908|Compound:7930301|FD-Score:3.47|P-value:1.03E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.02|P-value:6.25E-4 ID:SGTC_3147|Compound:9097027|FD-Score:6.97|P-value:5.67E-14 ID:SGTC_3149|Compound:9097855|FD-Score:8.67|P-value:1.37E-20 ID:SGTC_3151|Compound:9099821|FD-Score:-6.37|P-value:8.15E-12 ID:SGTC_3152|Compound:9099253|FD-Score:8.09|P-value:3.61E-18 ID:SGTC_3154|Compound:9100113|FD-Score:9.05|P-value:3.02E-22 ID:SGTC_3190|Compound:9110366|FD-Score:5.33|P-value:6.58E-9 ID:SGTC_3249|Compound:9135183|FD-Score:3.57|P-value:6.78E-5 ID:SGTC_3349|Compound:9149703|FD-Score:3.57|P-value:6.72E-5 ID:SGTC_3353|Compound:9151644|FD-Score:3.21|P-value:3.05E-4 ID:SGTC_117|Compound:lucigenin|FD-Score:2.90|P-value:9.47E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.03|P-value:8.55E-6 ID:SGTC_456|Compound:gingerol|FD-Score:-3.69|P-value:4.82E-5 ID:SGTC_521|Compound:r062-0005|FD-Score:2.90|P-value:9.46E-4 ID:SGTC_655|Compound:k060-0012|FD-Score:3.50|P-value:9.32E-5 ID:SGTC_732|Compound:3970-1727|FD-Score:2.92|P-value:9.01E-4 ID:SGTC_747|Compound:3448-8087|FD-Score:2.91|P-value:9.41E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.77|P-value:2.78E-5 ID:SGTC_866|Compound:0495-0101|FD-Score:3.68|P-value:4.33E-5 ID:SGTC_1266|Compound:0828-0227|FD-Score:-3.09|P-value:5.54E-4 ID:SGTC_1385|Compound:3091-4834|FD-Score:4.71|P-value:2.51E-7 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.11|P-value:5.29E-4 ID:SGTC_1389|Compound:3405-0187|FD-Score:-4.17|P-value:5.45E-6 ID:SGTC_1426|Compound:4048-4480|FD-Score:-2.96|P-value:9.04E-4 ID:SGTC_1520|Compound:flindersine|FD-Score:3.05|P-value:5.52E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:2.92|P-value:9.04E-4 ID:SGTC_1723|Compound:st034307|FD-Score:3.13|P-value:4.09E-4 ID:SGTC_1826|Compound:st055655|FD-Score:2.96|P-value:7.63E-4 ID:SGTC_1841|Compound:st054148|FD-Score:-3.00|P-value:7.97E-4 ID:SGTC_1843|Compound:st055809|FD-Score:3.22|P-value:2.88E-4 ID:SGTC_1982|Compound:st072467|FD-Score:3.08|P-value:4.98E-4 ID:SGTC_2113|Compound:5491891|FD-Score:3.48|P-value:9.98E-5 ID:SGTC_2124|Compound:5246320|FD-Score:3.45|P-value:1.13E-4 ID:SGTC_2183|Compound:6420625|FD-Score:3.23|P-value:2.81E-4 ID:SGTC_2190|Compound:6049624|FD-Score:3.35|P-value:1.72E-4 ID:SGTC_2303|Compound:7774051|FD-Score:3.61|P-value:5.85E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.54|P-value:7.97E-5 ID:SGTC_2528|Compound:xanthyletin|FD-Score:2.89|P-value:9.94E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-3.19|P-value:3.92E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:3.07|P-value:5.19E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.34|P-value:1.80E-4 ID:SGTC_2738|Compound:aclarubicin|FD-Score:3.16|P-value:3.67E-4 ID:SGTC_2880|Compound:9042983|FD-Score:3.30|P-value:2.10E-4 ID:SGTC_2887|Compound:9044784|FD-Score:3.63|P-value:5.18E-5 ID:SGTC_2908|Compound:7930301|FD-Score:3.47|P-value:1.03E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.02|P-value:6.25E-4 ID:SGTC_3147|Compound:9097027|FD-Score:6.97|P-value:5.67E-14 ID:SGTC_3149|Compound:9097855|FD-Score:8.67|P-value:1.37E-20 ID:SGTC_3151|Compound:9099821|FD-Score:-6.37|P-value:8.15E-12 ID:SGTC_3152|Compound:9099253|FD-Score:8.09|P-value:3.61E-18 ID:SGTC_3154|Compound:9100113|FD-Score:9.05|P-value:3.02E-22 ID:SGTC_3190|Compound:9110366|FD-Score:5.33|P-value:6.58E-9 ID:SGTC_3249|Compound:9135183|FD-Score:3.57|P-value:6.78E-5 ID:SGTC_3349|Compound:9149703|FD-Score:3.57|P-value:6.72E-5 ID:SGTC_3353|Compound:9151644|FD-Score:3.21|P-value:3.05E-4

Top fitness defect scores for YLL049W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_3154 9100113 9.05 49.5 µM 3.02E-22 3 7.28E-20
2 SGTC_3149 9097855 8.67 49.5 µM 1.37E-20 Golgi 4 2.13E-18
3 SGTC_3152 9099253 8.09 49.5 µM 3.61E-18 NEO1-PIK1 35 2.99E-16
4 SGTC_3147 9097027 6.97 49.5 µM 5.67E-14 3 1.57E-12
5 SGTC_3190 9110366 5.33 49.5 µM 6.58E-9 3 4.85E-8
6 SGTC_1385 3091-4834 4.71 14.1 µM 2.51E-7 RPP1 & pyrimidine depletion 6 1.22E-6
7 SGTC_241 5hr heat shock (37°C) + cantharidin 4.03 155.4 µM 8.55E-6 cell wall signaling 71 2.81E-5
8 SGTC_751 0833-0568 3.77 350.0 µM 2.78E-5 26 8.00E-5
9 SGTC_866 0495-0101 3.68 262.0 µM 4.33E-5 60S ribosome export 25 1.19E-4
10 SGTC_2887 9044784 3.63 58.4 µM 5.18E-5 fatty acid desaturase (OLE1) 41 1.39E-4
11 SGTC_2303 7774051 3.61 200.0 µM 5.85E-5 32 1.55E-4
12 SGTC_3349 9149703 3.57 23.0 µM 6.72E-5 23 1.75E-4
13 SGTC_3249 9135183 3.57 49.5 µM 6.78E-5 60S ribosome export 21 1.77E-4
14 SGTC_2418 pentamidine 3.54 31.7 µM 7.97E-5 mitochondrial processes 60 2.04E-4
15 SGTC_655 k060-0012 3.50 42.0 µM 9.32E-5 25 2.34E-4
16 SGTC_2113 5491891 3.48 107.0 µM 9.98E-5 TSC3-RPN4 41 2.49E-4
17 SGTC_2908 7930301 3.47 100.0 µM 1.03E-4 44 2.56E-4
18 SGTC_2124 5246320 3.45 200.0 µM 1.13E-4 heme biosynthesis & mitochondrial translocase 34 2.78E-4
19 SGTC_2190 6049624 3.35 200.0 µM 1.72E-4 11 4.04E-4
20 SGTC_2687 chenodiol 3.34 100.0 µM 1.80E-4 44 4.21E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.169 4.93E-23 YKR031C SPO14 hom Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
0.152 7.24E-19 YBR285W_p YBR285W_p hom Putative protein of unknown function; YBR285W is not an essential gene
0.105 9.32E-10 YDL068W_d YDL068W_d hom Dubious ORF unlikely to encode a protein, based on available experimental and comparative sequence data
0.102 3.59E-9 YMR079W SEC14 het Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs
0.101 4.34E-9 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.100 7.11E-9 YHR209W CRG1 hom S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
0.096 2.18E-8 YJR118C ILM1 hom Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
0.096 2.22E-8 YDR393W SHE9 hom Mitochondrial inner membrane protein required for normal mitochondrial morphology, may be involved in fission of the inner membrane; forms a homo-oligomeric complex
0.090 2.02E-7 YDR524C AGE1 hom ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector, involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
0.089 2.68E-7 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.087 3.97E-7 YPR040W TIP41 hom Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
0.087 4.25E-7 YCL054W SPB1 het AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
0.087 4.90E-7 YKL183W LOT5 hom Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
0.086 5.90E-7 YLR350W ORM2 hom Evolutionarily conserved protein, similar to Orm1p; required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress
0.085 7.87E-7 YOR245C DGA1 hom Diacylglycerol acyltransferase, catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor, localized to lipid particles