YLL050C / COF1

Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLL050C / COF1

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:2.99|P-value:3.98E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.88|P-value:6.77E-6 ID:SGTC_325|Compound:2810-4341|FD-Score:3.90|P-value:5.98E-6 ID:SGTC_326|Compound:k292-0812|FD-Score:3.15|P-value:2.10E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.11|P-value:2.23E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:2.85|P-value:7.01E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.73|P-value:1.46E-5 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.39|P-value:6.43E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:-3.96|P-value:3.88E-6 ID:SGTC_739|Compound:1486-1183|FD-Score:3.67|P-value:1.90E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.45|P-value:3.90E-10 ID:SGTC_827|Compound:3253-0474|FD-Score:3.36|P-value:8.37E-5 ID:SGTC_896|Compound:0964-0007|FD-Score:3.45|P-value:5.50E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.68|P-value:1.87E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.82|P-value:3.22E-8 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.15|P-value:1.61E-6 ID:SGTC_1267|Compound:0828-0272|FD-Score:-2.80|P-value:7.66E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.33|P-value:8.39E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.11|P-value:2.16E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.80|P-value:7.74E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.21|P-value:1.40E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.16|P-value:2.02E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:-2.85|P-value:6.34E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.32|P-value:8.94E-5 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.26|P-value:1.12E-4 ID:SGTC_1558|Compound:st069300|FD-Score:3.03|P-value:3.41E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.36|P-value:7.25E-5 ID:SGTC_1826|Compound:st055655|FD-Score:-2.86|P-value:6.20E-4 ID:SGTC_1899|Compound:st063254|FD-Score:-2.87|P-value:5.79E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.21|P-value:1.15E-6 ID:SGTC_2010|Compound:4012422|FD-Score:3.22|P-value:1.55E-4 ID:SGTC_2056|Compound:5235370|FD-Score:2.98|P-value:4.21E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.24|P-value:1.43E-4 ID:SGTC_2201|Compound:6605497|FD-Score:3.18|P-value:1.85E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.12|P-value:2.32E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-7.10|P-value:6.08E-16 ID:SGTC_2334|Compound:9017922|FD-Score:-2.93|P-value:4.57E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.19|P-value:1.58E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.79|P-value:9.01E-4 ID:SGTC_2476|Compound:5806916|FD-Score:3.66|P-value:2.00E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.92|P-value:4.74E-4 ID:SGTC_2798|Compound:7813637|FD-Score:-4.24|P-value:8.30E-7 ID:SGTC_2811|Compound:7967822|FD-Score:-3.11|P-value:2.22E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.38|P-value:6.68E-5 ID:SGTC_2884|Compound:9058502|FD-Score:2.80|P-value:8.53E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.04|P-value:2.94E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.46|P-value:4.73E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.04|P-value:2.97E-6 ID:SGTC_3291|Compound:9121177|FD-Score:3.02|P-value:3.51E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:2.99|P-value:3.98E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:3.88|P-value:6.77E-6 ID:SGTC_325|Compound:2810-4341|FD-Score:3.90|P-value:5.98E-6 ID:SGTC_326|Compound:k292-0812|FD-Score:3.15|P-value:2.10E-4 ID:SGTC_405|Compound:ebelactone b|FD-Score:-3.11|P-value:2.23E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:2.85|P-value:7.01E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:3.73|P-value:1.46E-5 ID:SGTC_735|Compound:3076-0345|FD-Score:-3.39|P-value:6.43E-5 ID:SGTC_738|Compound:1315-0003|FD-Score:-3.96|P-value:3.88E-6 ID:SGTC_739|Compound:1486-1183|FD-Score:3.67|P-value:1.90E-5 ID:SGTC_750|Compound:4073-0104|FD-Score:-5.45|P-value:3.90E-10 ID:SGTC_827|Compound:3253-0474|FD-Score:3.36|P-value:8.37E-5 ID:SGTC_896|Compound:0964-0007|FD-Score:3.45|P-value:5.50E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:3.68|P-value:1.87E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:4.82|P-value:3.22E-8 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.15|P-value:1.61E-6 ID:SGTC_1267|Compound:0828-0272|FD-Score:-2.80|P-value:7.66E-4 ID:SGTC_1288|Compound:0973-0013|FD-Score:-3.33|P-value:8.39E-5 ID:SGTC_1290|Compound:0986-0249|FD-Score:-3.11|P-value:2.16E-4 ID:SGTC_1300|Compound:1094-0077|FD-Score:-2.80|P-value:7.74E-4 ID:SGTC_1311|Compound:1190-0148|FD-Score:-3.21|P-value:1.40E-4 ID:SGTC_1345|Compound:1488-0192|FD-Score:3.16|P-value:2.02E-4 ID:SGTC_1387|Compound:3342-1166|FD-Score:-2.85|P-value:6.34E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:-3.32|P-value:8.94E-5 ID:SGTC_1549|Compound:4'-methoxyflavonol|FD-Score:-3.26|P-value:1.12E-4 ID:SGTC_1558|Compound:st069300|FD-Score:3.03|P-value:3.41E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-3.36|P-value:7.25E-5 ID:SGTC_1826|Compound:st055655|FD-Score:-2.86|P-value:6.20E-4 ID:SGTC_1899|Compound:st063254|FD-Score:-2.87|P-value:5.79E-4 ID:SGTC_1906|Compound:st060206|FD-Score:4.21|P-value:1.15E-6 ID:SGTC_2010|Compound:4012422|FD-Score:3.22|P-value:1.55E-4 ID:SGTC_2056|Compound:5235370|FD-Score:2.98|P-value:4.21E-4 ID:SGTC_2103|Compound:5378138|FD-Score:3.24|P-value:1.43E-4 ID:SGTC_2201|Compound:6605497|FD-Score:3.18|P-value:1.85E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.12|P-value:2.32E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-7.10|P-value:6.08E-16 ID:SGTC_2334|Compound:9017922|FD-Score:-2.93|P-value:4.57E-4 ID:SGTC_2353|Compound:9059630|FD-Score:-3.19|P-value:1.58E-4 ID:SGTC_2435|Compound:5706476|FD-Score:2.79|P-value:9.01E-4 ID:SGTC_2476|Compound:5806916|FD-Score:3.66|P-value:2.00E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:-2.92|P-value:4.74E-4 ID:SGTC_2798|Compound:7813637|FD-Score:-4.24|P-value:8.30E-7 ID:SGTC_2811|Compound:7967822|FD-Score:-3.11|P-value:2.22E-4 ID:SGTC_2833|Compound:9001315|FD-Score:-3.38|P-value:6.68E-5 ID:SGTC_2884|Compound:9058502|FD-Score:2.80|P-value:8.53E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-3.04|P-value:2.94E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.46|P-value:4.73E-5 ID:SGTC_3202|Compound:9113761|FD-Score:4.04|P-value:2.97E-6 ID:SGTC_3291|Compound:9121177|FD-Score:3.02|P-value:3.51E-4

Top fitness defect scores for YLL050C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1167 k781-0610 4.82 148.0 µM 3.22E-8 RNA processing & uracil transport 3 7.26E-7 0.20
2 SGTC_1906 st060206 4.21 94.7 µM 1.15E-6 2 1.27E-5 0.28
3 SGTC_1214 0358-0031 4.15 63.7 µM 1.61E-6 4 1.66E-5 0.01
4 SGTC_3202 9113761 4.04 49.5 µM 2.97E-6 10 2.70E-5 0.01
5 SGTC_325 2810-4341 3.90 181.0 µM 5.98E-6 amide catabolism 6 4.72E-5 0.05
6 SGTC_234 nsc-17383 3.88 10.7 µM 6.77E-6 ERG2 5 5.22E-5 0.42
7 SGTC_734 2023-0417 3.73 80.0 µM 1.46E-5 ergosterol biosynthesis 8 9.66E-5 0.14
8 SGTC_997 3996-0078 3.68 137.0 µM 1.87E-5 10 1.18E-4 0.08
9 SGTC_739 1486-1183 3.67 203.0 µM 1.90E-5 3 1.19E-4 0.31
10 SGTC_2476 5806916 3.66 10.5 µM 2.00E-5 3 1.25E-4 0.13
11 SGTC_896 0964-0007 3.45 44.5 µM 5.50E-5 13 2.80E-4 0.02
12 SGTC_827 3253-0474 3.36 693.0 µM 8.37E-5 12 3.92E-4 0.27
13 SGTC_2103 5378138 3.24 10.0 µM 1.43E-4 5 6.04E-4 0.03
14 SGTC_2010 4012422 3.22 159.0 µM 1.55E-4 9 6.45E-4 0.30
15 SGTC_2201 6605497 3.18 169.9 µM 1.85E-4 ergosterol biosynthesis 9 7.41E-4 0.06
16 SGTC_1345 1488-0192 3.16 31.4 µM 2.02E-4 5 7.95E-4 0.04
17 SGTC_326 k292-0812 3.15 17.5 µM 2.10E-4 8 8.21E-4 0.27
18 SGTC_2331 7980721 3.12 200.0 µM 2.32E-4 22 8.92E-4 0.07
19 SGTC_1558 st069300 3.03 45.6 µM 3.41E-4 10 0.00121 0.12
20 SGTC_3291 9121177 3.02 71.4 µM 3.51E-4 6 0.00124 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.34E-18 YKL140W TGL1 hom Steryl ester hydrolase, one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
0.148 6.84E-18 YLL038C ENT4 hom Protein of unknown function, contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
0.147 1.35E-17 YEL004W YEA4 hom Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter required for cell wall chitin synthesis; localized to the ER
0.146 2.27E-17 YLR223C IFH1 het Coactivator that regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing
0.137 1.71E-15 YMR210W YMR210W hom Putative acyltransferase with similarity to Eeb1p and Eht1p, has a minor role in medium-chain fatty acid ethyl ester biosynthesis; may be involved in lipid metabolism and detoxification
0.130 5.18E-14 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.125 3.69E-13 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.123 7.94E-13 YKL131W_d YKL131W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.121 2.21E-12 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.116 1.45E-11 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.112 9.13E-11 YJR012C_p YJR012C_p het Essential protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; partially overlaps neighboring ORF, GPI14/YJR013W
0.111 1.05E-10 YPR010C RPA135 het RNA polymerase I second largest subunit A135
0.110 1.57E-10 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.110 1.88E-10 YJR056C YJR056C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
0.109 2.12E-10 YBR118W TEF2 hom Translational elongation factor EF-1 alpha; also encoded by TEF1; functions in the binding reaction of aminoacyl-tRNA (AA-tRNA) to ribosomes; may also have a role in tRNA re-export from the nucleus; TEF2 has a paralog, TEF1, that arose from the whole genome duplication