HIPHOP chemogenomics database

YLL062C / MHT1

S-methylmethionine-homocysteine methyltransferase, functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio

Zygosity: Homozygous strain

fixedexpanded
Profile for YLL062C / MHT1

Click on Significant Values for Screen Details

Top fitness defect scores for YLL062C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1239	0337-0584	5.37	35.4 µM	1.58E-8	unfolded protein response	22	4.01E-8
2	SGTC_693	4161-2696	4.37	114.0 µM	3.30E-6		4	6.13E-6
3	SGTC_1741	st038134	4.07	10.2 µM	1.35E-5		28	2.31E-5
4	SGTC_1033	k072-0102	3.97	21.3 µM	2.12E-5	60S ribosome export	21	3.54E-5
5	SGTC_3329	9141233	3.77	27.6 µM	5.23E-5		18	8.28E-5
6	SGTC_2611	pyromycin	3.70	23.9 µM	6.99E-5		33	1.09E-4
7	SGTC_2606	farnesol	3.68	9.8 µM	7.57E-5		25	1.17E-4
8	SGTC_2419	paclitaxel	3.59	166.5 µM	1.09E-4		58	1.66E-4
9	SGTC_1044	1222-0021	3.45	1.8 µM	1.93E-4	heme biosynthesis & mitochondrial translocase	53	2.84E-4
10	SGTC_366	0181-0206	3.33	4.3 µM	3.01E-4	Golgi	71	4.32E-4
11	SGTC_2484	5809818	3.19	12.0 µM	5.03E-4		37	7.01E-4
12	SGTC_3331	9141060	3.14	2.0 µM	6.19E-4	cell wall	61	8.52E-4
13	SGTC_2857	9024691	3.12	58.4 µM	6.62E-4		61	9.09E-4
14	SGTC_1428	4049-0204	3.06	61.7 µM	8.26E-4		45	0.00112
15	SGTC_2974	9094440	3.01	9.7 µM	9.66E-4	ergosterol biosynthesis	96	0.00130
16	SGTC_2294	7971431	2.98	200.0 µM	0.00107		54	0.00143
17	SGTC_2762	leflunomide	2.98	42.1 µM	0.00109		78	0.00145
18	SGTC_1032	k061-1018	2.89	98.7 µM	0.00148		82	0.00195
19	SGTC_656	4090-1979	2.86	187.0 µM	0.00161		79	0.00210
20	SGTC_2159	5658611	2.72	73.8 µM	0.00255		43	0.00325

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.232	2.11E-42	YML130C	ERO1	het	Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.207	6.80E-34	YFL032W_d	YFL032W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.148	7.04E-18	YFL028C	CAF16	hom	Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
0.135	5.00E-15	YGR025W_d	YGR025W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.128	8.62E-14	YGL156W	AMS1	hom	Vacuolar alpha mannosidase, involved in free oligosaccharide (fOS) degradation; delivered to the vacuole in a novel pathway separate from the secretory pathway
0.128	9.62E-14	YLR132C	USB1	het	Putative phosphodiesterase specific for U6 snRNA 3' end modification; trims the 3' poly(u) tract to leave a terminal 3' phosphate; human homolog, hUSB1 (aka C16orf57) produces a 2',3' cyclic phosphate; mutations in hUSB1 are associated with a rare skin condition (OMIM 604173); essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants
0.125	3.20E-13	YJR152W	DAL5	hom	Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
0.122	1.17E-12	YBL028C	YBL028C	hom	Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
0.121	2.10E-12	YDR206W	EBS1	hom	Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.119	4.51E-12	YHR079C	IRE1	hom	Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; Kar2p binds inactive Ire1p and releases from it upon ER stress
0.118	7.62E-12	YJR061W_p	YJR061W_p	hom	Putative protein of unknown function; non-essential gene; transcription repressed by Rm101p; YJR061W has a paralog, MNN4, that arose from the whole genome duplication
0.113	6.24E-11	YLL060C	GTT2	hom	Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
0.113	6.65E-11	YKL184W	SPE1	hom	Ornithine decarboxylase, catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
0.111	1.37E-10	YHL012W_p	YHL012W_p	hom	Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
0.110	1.61E-10	YGL170C	SPO74	hom	Component of the meiotic outer plaque of the spindle pole body, involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation