YLR005W / SSL1

Component of the core form of RNA polymerase transcription factor TFIIH, which has both protein kinase and DNA-dependent ATPase/helicase activities and is essential for transcription and nucleotide excision repair; interacts with Tfb4p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR005W / SSL1

Click on Significant Values for Screen Details ID:SGTC_168|Compound:3970-0862|FD-Score:3.83|P-value:2.86E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.44|P-value:9.77E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-3.37|P-value:9.60E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.82|P-value:2.88E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.01|P-value:1.50E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:4.23|P-value:6.98E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:-4.45|P-value:2.21E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:4.02|P-value:1.44E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.36|P-value:9.98E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.37|P-value:4.06E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.55|P-value:7.05E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:5.26|P-value:1.04E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.85|P-value:2.64E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-4.84|P-value:4.44E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:-4.09|P-value:8.73E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.38|P-value:9.44E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.62|P-value:5.58E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.45|P-value:3.04E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.30|P-value:5.31E-5 ID:SGTC_2179|Compound:6298986|FD-Score:3.57|P-value:6.49E-4 ID:SGTC_2291|Compound:7917025|FD-Score:4.94|P-value:4.11E-6 ID:SGTC_2312|Compound:7706453|FD-Score:3.86|P-value:2.51E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.50|P-value:8.16E-4 ID:SGTC_2412|Compound:st077135|FD-Score:6.36|P-value:4.46E-9 ID:SGTC_2418|Compound:pentamidine|FD-Score:11.50|P-value:6.94E-26 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.73|P-value:9.73E-6 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.99|P-value:1.22E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.33|P-value:7.42E-7 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:6.49|P-value:2.14E-9 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.63|P-value:5.52E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.13|P-value:1.50E-8 ID:SGTC_2625|Compound:euphol|FD-Score:-3.65|P-value:3.92E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.52|P-value:3.05E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.16|P-value:1.60E-6 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.67|P-value:4.73E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.59|P-value:6.09E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.79|P-value:3.24E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.15|P-value:9.13E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:9.35|P-value:1.19E-17 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.07|P-value:1.21E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.88|P-value:5.31E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:4.09|P-value:1.15E-4 ID:SGTC_2822|Compound:7994718|FD-Score:3.44|P-value:9.86E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.20|P-value:7.64E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.58|P-value:6.30E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.92|P-value:1.55E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.84|P-value:2.71E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.86|P-value:2.52E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:3.83|P-value:2.86E-4 ID:SGTC_177|Compound:1327-0068|FD-Score:3.44|P-value:9.77E-4 ID:SGTC_245|Compound:methotrexate|FD-Score:-3.37|P-value:9.60E-4 ID:SGTC_489|Compound:paxilline|FD-Score:3.82|P-value:2.88E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:4.01|P-value:1.50E-4 ID:SGTC_646|Compound:4092-0839|FD-Score:4.23|P-value:6.98E-5 ID:SGTC_770|Compound:0389-1121|FD-Score:-4.45|P-value:2.21E-5 ID:SGTC_928|Compound:2764-0428|FD-Score:4.02|P-value:1.44E-4 ID:SGTC_950|Compound:1165-0508|FD-Score:-3.36|P-value:9.98E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:4.37|P-value:4.06E-5 ID:SGTC_1177|Compound:0983-0204|FD-Score:3.55|P-value:7.05E-4 ID:SGTC_1465|Compound:k213-0056|FD-Score:5.26|P-value:1.04E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.85|P-value:2.64E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:-4.84|P-value:4.44E-6 ID:SGTC_1514|Compound:1189-1591|FD-Score:-4.09|P-value:8.73E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:-3.38|P-value:9.44E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.62|P-value:5.58E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:4.45|P-value:3.04E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.30|P-value:5.31E-5 ID:SGTC_2179|Compound:6298986|FD-Score:3.57|P-value:6.49E-4 ID:SGTC_2291|Compound:7917025|FD-Score:4.94|P-value:4.11E-6 ID:SGTC_2312|Compound:7706453|FD-Score:3.86|P-value:2.51E-4 ID:SGTC_2344|Compound:7990960|FD-Score:3.50|P-value:8.16E-4 ID:SGTC_2412|Compound:st077135|FD-Score:6.36|P-value:4.46E-9 ID:SGTC_2418|Compound:pentamidine|FD-Score:11.50|P-value:6.94E-26 ID:SGTC_2490|Compound:carminic acid|FD-Score:4.73|P-value:9.73E-6 ID:SGTC_2506|Compound:ethyl hematommate|FD-Score:-3.99|P-value:1.22E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:5.33|P-value:7.42E-7 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:6.49|P-value:2.14E-9 ID:SGTC_2560|Compound:caryophyllene|FD-Score:3.63|P-value:5.52E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:6.13|P-value:1.50E-8 ID:SGTC_2625|Compound:euphol|FD-Score:-3.65|P-value:3.92E-4 ID:SGTC_2645|Compound:abamectin|FD-Score:5.52|P-value:3.05E-7 ID:SGTC_2651|Compound:1327-0036|FD-Score:5.16|P-value:1.60E-6 ID:SGTC_2659|Compound:sanguinarine|FD-Score:3.67|P-value:4.73E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:3.59|P-value:6.09E-4 ID:SGTC_2722|Compound:naftopidil|FD-Score:3.79|P-value:3.24E-4 ID:SGTC_2764|Compound:methyldopa|FD-Score:4.15|P-value:9.13E-5 ID:SGTC_2766|Compound:oltipraz|FD-Score:9.35|P-value:1.19E-17 ID:SGTC_2767|Compound:sibutramine|FD-Score:4.07|P-value:1.21E-4 ID:SGTC_2768|Compound:sulfasalazine|FD-Score:4.88|P-value:5.31E-6 ID:SGTC_2777|Compound:climbazole|FD-Score:4.09|P-value:1.15E-4 ID:SGTC_2822|Compound:7994718|FD-Score:3.44|P-value:9.86E-4 ID:SGTC_2823|Compound:7987624|FD-Score:4.20|P-value:7.64E-5 ID:SGTC_2968|Compound:9090663|FD-Score:3.58|P-value:6.30E-4 ID:SGTC_2975|Compound:9084847|FD-Score:-3.92|P-value:1.55E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.84|P-value:2.71E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.86|P-value:2.52E-4

Top fitness defect scores for YLR005W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2418 pentamidine 11.50 31.7 µM 6.94E-26 mitochondrial processes 1 4.64E-31 1.87
2 SGTC_2766 oltipraz 9.35 83.9 µM 1.19E-17 heme requiring 6 4.28E-21 0.83
3 SGTC_2546 rhodinyl acetate 6.49 100.0 µM 2.14E-9 2 4.16E-11 1.01
4 SGTC_2412 st077135 6.36 33.4 µM 4.46E-9 1 1.01E-10 1.04
5 SGTC_2575 plumbagin 6.13 510.0 nM 1.50E-8 superoxide 10 4.38E-10 0.03
6 SGTC_2645 abamectin 5.52 100.0 µM 3.05E-7 heme requiring 4 1.68E-8 0.04
7 SGTC_2544 sphondin 5.33 68.5 µM 7.42E-7 RNA pol III & RNase P/MRP 23 4.91E-8 0.22
8 SGTC_1465 k213-0056 5.26 144.0 µM 1.04E-6 DNA intercalators 4 7.36E-8 0.41
9 SGTC_2651 1327-0036 5.16 5.0 µM 1.60E-6 38 1.25E-7 0.06
10 SGTC_2291 7917025 4.94 104.3 µM 4.11E-6 iron homeostasis 7 3.90E-7 0.04
11 SGTC_2768 sulfasalazine 4.88 50.2 µM 5.31E-6 4 5.30E-7 0.01
12 SGTC_2490 carminic acid 4.73 95.4 µM 9.73E-6 4 1.10E-6 0.22
13 SGTC_1873 rapamycin 4.45 2.0 nM 3.04E-5 13 4.37E-6 0.06
14 SGTC_1113 artemisinin 4.37 1.2 µM 4.06E-5 heme biosynthesis & mitochondrial translocase 30 6.20E-6 0.27
15 SGTC_1964 st076914 4.30 61.0 µM 5.31E-5 RPP1 & pyrimidine depletion 5 8.56E-6 0.10
16 SGTC_646 4092-0839 4.23 27.3 µM 6.98E-5 plasma membrane duress 8 1.19E-5 0.12
17 SGTC_2823 7987624 4.20 58.4 µM 7.64E-5 9 1.33E-5 0.05
18 SGTC_2764 methyldopa 4.15 94.7 µM 9.13E-5 3 1.65E-5 0.34
19 SGTC_2777 climbazole 4.09 1.5 µM 1.15E-4 iron homeostasis 4 2.18E-5 0.20
20 SGTC_2767 sibutramine 4.07 71.5 µM 1.21E-4 1 2.31E-5 0.72

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.188 4.01E-28 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.138 1.02E-15 YMR058W FET3 hom Ferro-O2-oxidoreductase; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
0.127 1.87E-13 YJL031C BET4 het Alpha subunit of Type II geranylgeranyltransferase required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
0.118 7.82E-12 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.111 1.15E-10 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.109 2.82E-10 YML091C RPM2 het Protein subunit of mitochondrial RNase P, has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
0.103 2.24E-9 YBL030C PET9 het Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; phosphorylated; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication
0.103 2.25E-9 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.101 4.96E-9 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.100 7.60E-9 YIL135C VHS2 hom Cytoplasmic protein of unknown function; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; similar to Mlf3p
0.099 8.38E-9 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.098 1.28E-8 YPR148C YPR148C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.097 1.60E-8 YPL204W HRR25 het Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)
0.095 3.57E-8 YHR082C KSP1 hom Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
0.093 7.70E-8 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus