YLR008C / PAM18

Constituent of the import motor (PAM complex) component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YLR008C / PAM18

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.05|P-value:2.31E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.70|P-value:1.04E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:3.15|P-value:1.46E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.74|P-value:8.48E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:2.91|P-value:4.07E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.03|P-value:2.34E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.63|P-value:1.49E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.85|P-value:5.27E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-3.91|P-value:3.21E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:3.39|P-value:4.93E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:2.75|P-value:8.00E-4 ID:SGTC_394|Compound:menadione|FD-Score:2.76|P-value:7.52E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:5.41|P-value:2.34E-10 ID:SGTC_567|Compound:1080-0542|FD-Score:4.28|P-value:4.32E-7 ID:SGTC_585|Compound:0kpi-0023|FD-Score:2.75|P-value:7.79E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.19|P-value:1.25E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:6.67|P-value:7.85E-15 ID:SGTC_816|Compound:0325-0417|FD-Score:3.74|P-value:8.63E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.61|P-value:1.71E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:3.45|P-value:3.69E-5 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.04|P-value:2.43E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:-2.93|P-value:3.46E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-2.77|P-value:6.88E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.92|P-value:3.30E-6 ID:SGTC_1759|Compound:st043059|FD-Score:2.95|P-value:3.44E-4 ID:SGTC_1830|Compound:st055982|FD-Score:2.76|P-value:7.61E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.26|P-value:8.90E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-4.75|P-value:2.09E-8 ID:SGTC_2006|Compound:4011487|FD-Score:3.48|P-value:3.18E-5 ID:SGTC_2096|Compound:5468907|FD-Score:2.96|P-value:3.31E-4 ID:SGTC_2101|Compound:5377231|FD-Score:2.82|P-value:6.07E-4 ID:SGTC_2140|Compound:5586273|FD-Score:2.85|P-value:5.40E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.37|P-value:4.83E-5 ID:SGTC_2182|Compound:6325192|FD-Score:2.87|P-value:4.87E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.16|P-value:1.39E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.39|P-value:4.87E-5 ID:SGTC_2434|Compound:5670830|FD-Score:2.71|P-value:9.40E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-2.91|P-value:3.79E-4 ID:SGTC_2585|Compound:alliin|FD-Score:-4.73|P-value:2.33E-8 ID:SGTC_2587|Compound:piperic acid|FD-Score:-4.65|P-value:3.94E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.60|P-value:5.92E-8 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.19|P-value:1.13E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.69|P-value:9.89E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.95|P-value:3.51E-4 ID:SGTC_2717|Compound:st081029|FD-Score:2.85|P-value:5.36E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.14|P-value:1.45E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.37|P-value:4.87E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-3.58|P-value:1.73E-5 ID:SGTC_2827|Compound:7993847|FD-Score:6.81|P-value:2.20E-15 ID:SGTC_2888|Compound:9058547|FD-Score:2.87|P-value:4.97E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.54|P-value:2.13E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-3.14|P-value:1.44E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-2.85|P-value:5.03E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-3.10|P-value:1.71E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.01|P-value:2.49E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.34|P-value:6.05E-5 ID:SGTC_3116|Compound:9124943|FD-Score:-2.98|P-value:2.85E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.29|P-value:7.74E-5 ID:SGTC_3153|Compound:9100258|FD-Score:2.75|P-value:8.01E-4 ID:SGTC_3166|Compound:9102297|FD-Score:2.96|P-value:3.31E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.05|P-value:2.31E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:3.70|P-value:1.04E-5 ID:SGTC_67|Compound:0335-0847|FD-Score:3.15|P-value:1.46E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:3.74|P-value:8.48E-6 ID:SGTC_158|Compound:k038-0087|FD-Score:2.91|P-value:4.07E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:-3.03|P-value:2.34E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.63|P-value:1.49E-5 ID:SGTC_294|Compound:k038-0071|FD-Score:2.85|P-value:5.27E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-3.91|P-value:3.21E-6 ID:SGTC_340|Compound:k038-0059|FD-Score:3.39|P-value:4.93E-5 ID:SGTC_352|Compound:k048-0007|FD-Score:2.75|P-value:8.00E-4 ID:SGTC_394|Compound:menadione|FD-Score:2.76|P-value:7.52E-4 ID:SGTC_395|Compound:anisomycin|FD-Score:5.41|P-value:2.34E-10 ID:SGTC_567|Compound:1080-0542|FD-Score:4.28|P-value:4.32E-7 ID:SGTC_585|Compound:0kpi-0023|FD-Score:2.75|P-value:7.79E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.19|P-value:1.25E-4 ID:SGTC_813|Compound:k007-0158|FD-Score:6.67|P-value:7.85E-15 ID:SGTC_816|Compound:0325-0417|FD-Score:3.74|P-value:8.63E-6 ID:SGTC_817|Compound:1391-0715|FD-Score:3.61|P-value:1.71E-5 ID:SGTC_822|Compound:1487-0950|FD-Score:3.45|P-value:3.69E-5 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.04|P-value:2.43E-4 ID:SGTC_1431|Compound:4049-0260|FD-Score:-2.93|P-value:3.46E-4 ID:SGTC_1536|Compound:cytidine-2',3'-monophosphoric acid;  3'-cytidylic acid|FD-Score:-2.77|P-value:6.88E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.92|P-value:3.30E-6 ID:SGTC_1759|Compound:st043059|FD-Score:2.95|P-value:3.44E-4 ID:SGTC_1830|Compound:st055982|FD-Score:2.76|P-value:7.61E-4 ID:SGTC_1881|Compound:5652484|FD-Score:3.26|P-value:8.90E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-4.75|P-value:2.09E-8 ID:SGTC_2006|Compound:4011487|FD-Score:3.48|P-value:3.18E-5 ID:SGTC_2096|Compound:5468907|FD-Score:2.96|P-value:3.31E-4 ID:SGTC_2101|Compound:5377231|FD-Score:2.82|P-value:6.07E-4 ID:SGTC_2140|Compound:5586273|FD-Score:2.85|P-value:5.40E-4 ID:SGTC_2162|Compound:5663267|FD-Score:-3.37|P-value:4.83E-5 ID:SGTC_2182|Compound:6325192|FD-Score:2.87|P-value:4.87E-4 ID:SGTC_2307|Compound:7538926|FD-Score:3.16|P-value:1.39E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.39|P-value:4.87E-5 ID:SGTC_2434|Compound:5670830|FD-Score:2.71|P-value:9.40E-4 ID:SGTC_2480|Compound:5767542|FD-Score:-2.91|P-value:3.79E-4 ID:SGTC_2585|Compound:alliin|FD-Score:-4.73|P-value:2.33E-8 ID:SGTC_2587|Compound:piperic acid|FD-Score:-4.65|P-value:3.94E-8 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:4.60|P-value:5.92E-8 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.19|P-value:1.13E-4 ID:SGTC_2620|Compound:irigenin|FD-Score:2.69|P-value:9.89E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:2.95|P-value:3.51E-4 ID:SGTC_2717|Compound:st081029|FD-Score:2.85|P-value:5.36E-4 ID:SGTC_2750|Compound:bromebric acid|FD-Score:-3.14|P-value:1.45E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:-3.37|P-value:4.87E-5 ID:SGTC_2826|Compound:7992541|FD-Score:-3.58|P-value:1.73E-5 ID:SGTC_2827|Compound:7993847|FD-Score:6.81|P-value:2.20E-15 ID:SGTC_2888|Compound:9058547|FD-Score:2.87|P-value:4.97E-4 ID:SGTC_2925|Compound:9010536|FD-Score:-3.54|P-value:2.13E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-3.14|P-value:1.44E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-2.85|P-value:5.03E-4 ID:SGTC_2980|Compound:9014342|FD-Score:-3.10|P-value:1.71E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.01|P-value:2.49E-4 ID:SGTC_3110|Compound:9122132|FD-Score:3.34|P-value:6.05E-5 ID:SGTC_3116|Compound:9124943|FD-Score:-2.98|P-value:2.85E-4 ID:SGTC_3148|Compound:9097893|FD-Score:3.29|P-value:7.74E-5 ID:SGTC_3153|Compound:9100258|FD-Score:2.75|P-value:8.01E-4 ID:SGTC_3166|Compound:9102297|FD-Score:2.96|P-value:3.31E-4

Top fitness defect scores for YLR008C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2827 7993847 6.81 71.4 µM 2.20E-15 2 4.72E-12 2.16
2 SGTC_813 k007-0158 6.67 173.0 µM 7.85E-15 fatty acid desaturase (OLE1) 1 1.24E-11 1.14
3 SGTC_395 anisomycin 5.41 6.2 µM 2.34E-10 1 3.07E-8 0.89
4 SGTC_2588 indole-3-carbinol 4.60 100.0 µM 5.92E-8 3 2.06E-6 0.31
5 SGTC_567 1080-0542 4.28 68.4 µM 4.32E-7 2 9.39E-6 0.16
6 SGTC_1731 st035964 3.92 63.0 µM 3.30E-6 11 4.43E-5 0.07
7 SGTC_103 0844-0104 3.74 51.8 µM 8.48E-6 ERAD & cell cycle 52 9.14E-5 0.02
8 SGTC_816 0325-0417 3.74 81.3 µM 8.63E-6 4 9.26E-5 0.02
9 SGTC_64 000l-0018 3.70 272.1 µM 1.04E-5 ubiquinone biosynthesis & proteasome 51 1.07E-4 0.00
10 SGTC_216 k038-0035 3.63 28.4 µM 1.49E-5 ubiquinone biosynthesis & proteasome 17 1.41E-4 0.04
11 SGTC_817 1391-0715 3.61 46.0 µM 1.71E-5 11 1.56E-4 0.06
12 SGTC_2006 4011487 3.48 200.0 µM 3.18E-5 endomembrane recycling 22 2.51E-4 0.01
13 SGTC_822 1487-0950 3.45 154.0 µM 3.69E-5 5 2.82E-4 0.10
14 SGTC_2398 5228213 3.39 48.8 µM 4.87E-5 iron homeostasis 20 3.48E-4 0.09
15 SGTC_340 k038-0059 3.39 454.0 µM 4.93E-5 ubiquinone biosynthesis & proteasome 12 3.52E-4 0.10
16 SGTC_3110 9122132 3.34 49.5 µM 6.05E-5 redox potentiating 16 4.12E-4 0.10
17 SGTC_3148 9097893 3.29 49.5 µM 7.74E-5 16 4.97E-4 0.03
18 SGTC_1881 5652484 3.26 16.0 µM 8.90E-5 TRP & mitochondrial translation 15 5.54E-4 0.26
19 SGTC_600 1495-0418 3.19 41.6 µM 1.25E-4 RPP1 & pyrimidine depletion 13 7.21E-4 0.10
20 SGTC_2307 7538926 3.16 6.3 µM 1.39E-4 35 7.82E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.189 2.41E-28 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.164 1.39E-21 YMR110C HFD1 hom Hexadecenal dehydrogenase; involved in the conversion of sphingosine 1-phosphate breakdown product hexadecenal to hexadecenoic acid; located in the mitochondrial outer membrane and also in lipid particles; has similarity to ALDH3A2, a human fatty aldehyde dehydrogenase (FALDH) mutated in Sjogren-Larsson syndrome, a neurocutaneous disorder
0.157 7.73E-20 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.147 9.82E-18 YPR146C_d YPR146C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.139 5.54E-16 YFR007W YFH7 hom Putative kinase with similarity to the phosphoribulokinase/uridine kinase/bacterial pantothenate kinase (PRK/URK/PANK) subfamily of P-loop kinases
0.138 1.06E-15 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.137 1.66E-15 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
0.135 4.47E-15 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.132 1.35E-14 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.129 5.33E-14 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.124 5.11E-13 YDL020C RPN4 hom Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
0.123 9.18E-13 YLR199C PBA1 hom Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
0.118 6.67E-12 YJL168C SET2 hom Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p
0.117 8.56E-12 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.117 9.57E-12 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER