YLR014C / PPR1

Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR014C / PPR1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.00|P-value:2.03E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.52|P-value:4.80E-7 ID:SGTC_154|Compound:3389-1638|FD-Score:3.75|P-value:4.50E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.76|P-value:1.22E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.72|P-value:4.96E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:6.23|P-value:1.55E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:4.95|P-value:5.73E-6 ID:SGTC_321|Compound:4534-3890|FD-Score:4.97|P-value:5.33E-6 ID:SGTC_330|Compound:1488-0319|FD-Score:4.92|P-value:6.52E-6 ID:SGTC_333|Compound:3486-0131|FD-Score:4.53|P-value:3.02E-5 ID:SGTC_336|Compound:4111-0029|FD-Score:4.60|P-value:2.30E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:5.13|P-value:2.66E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.76|P-value:3.57E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.23|P-value:1.78E-6 ID:SGTC_847|Compound:0342-0274|FD-Score:-4.05|P-value:1.35E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.94|P-value:2.44E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.69|P-value:1.65E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-5.58|P-value:2.71E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.55|P-value:6.98E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.73|P-value:4.89E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:6.67|P-value:1.59E-9 ID:SGTC_1350|Compound:1495-0454|FD-Score:-4.04|P-value:1.37E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:6.35|P-value:8.41E-9 ID:SGTC_1401|Compound:3807-4679|FD-Score:-4.47|P-value:2.94E-5 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.54|P-value:8.69E-4 ID:SGTC_1745|Compound:st081495|FD-Score:8.46|P-value:2.79E-14 ID:SGTC_1875|Compound:nocodazole|FD-Score:-3.60|P-value:5.93E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.55|P-value:8.54E-4 ID:SGTC_2165|Compound:5727197|FD-Score:5.04|P-value:3.93E-6 ID:SGTC_2192|Compound:5915882|FD-Score:9.58|P-value:7.64E-18 ID:SGTC_2248|Compound:tofa|FD-Score:4.28|P-value:7.48E-5 ID:SGTC_2305|Compound:7443522|FD-Score:-4.12|P-value:1.05E-4 ID:SGTC_2313|Compound:7726885|FD-Score:5.36|P-value:9.92E-7 ID:SGTC_2342|Compound:7990637|FD-Score:3.81|P-value:3.72E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.64|P-value:5.16E-4 ID:SGTC_2416|Compound:tofa|FD-Score:5.63|P-value:2.93E-7 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.67|P-value:4.79E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.53|P-value:9.00E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.52|P-value:9.28E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.00|P-value:2.03E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:5.52|P-value:4.80E-7 ID:SGTC_154|Compound:3389-1638|FD-Score:3.75|P-value:4.50E-4 ID:SGTC_207|Compound:4185-0010|FD-Score:4.76|P-value:1.22E-5 ID:SGTC_256|Compound:clotrimazole|FD-Score:3.72|P-value:4.96E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:6.23|P-value:1.55E-8 ID:SGTC_320|Compound:4092-0846|FD-Score:4.95|P-value:5.73E-6 ID:SGTC_321|Compound:4534-3890|FD-Score:4.97|P-value:5.33E-6 ID:SGTC_330|Compound:1488-0319|FD-Score:4.92|P-value:6.52E-6 ID:SGTC_333|Compound:3486-0131|FD-Score:4.53|P-value:3.02E-5 ID:SGTC_336|Compound:4111-0029|FD-Score:4.60|P-value:2.30E-5 ID:SGTC_340|Compound:k038-0059|FD-Score:5.13|P-value:2.66E-6 ID:SGTC_649|Compound:1123-0311|FD-Score:-3.76|P-value:3.57E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:5.23|P-value:1.78E-6 ID:SGTC_847|Compound:0342-0274|FD-Score:-4.05|P-value:1.35E-4 ID:SGTC_926|Compound:2268-1401|FD-Score:3.94|P-value:2.44E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:-5.69|P-value:1.65E-7 ID:SGTC_938|Compound:3126-1755|FD-Score:-5.58|P-value:2.71E-7 ID:SGTC_1251|Compound:0490-5699|FD-Score:-3.55|P-value:6.98E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.73|P-value:4.89E-4 ID:SGTC_1348|Compound:1493-0373|FD-Score:6.67|P-value:1.59E-9 ID:SGTC_1350|Compound:1495-0454|FD-Score:-4.04|P-value:1.37E-4 ID:SGTC_1364|Compound:1653-0028|FD-Score:6.35|P-value:8.41E-9 ID:SGTC_1401|Compound:3807-4679|FD-Score:-4.47|P-value:2.94E-5 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.54|P-value:8.69E-4 ID:SGTC_1745|Compound:st081495|FD-Score:8.46|P-value:2.79E-14 ID:SGTC_1875|Compound:nocodazole|FD-Score:-3.60|P-value:5.93E-4 ID:SGTC_1963|Compound:st076565|FD-Score:3.55|P-value:8.54E-4 ID:SGTC_2165|Compound:5727197|FD-Score:5.04|P-value:3.93E-6 ID:SGTC_2192|Compound:5915882|FD-Score:9.58|P-value:7.64E-18 ID:SGTC_2248|Compound:tofa|FD-Score:4.28|P-value:7.48E-5 ID:SGTC_2305|Compound:7443522|FD-Score:-4.12|P-value:1.05E-4 ID:SGTC_2313|Compound:7726885|FD-Score:5.36|P-value:9.92E-7 ID:SGTC_2342|Compound:7990637|FD-Score:3.81|P-value:3.72E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:-3.64|P-value:5.16E-4 ID:SGTC_2416|Compound:tofa|FD-Score:5.63|P-value:2.93E-7 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.67|P-value:4.79E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:3.53|P-value:9.00E-4 ID:SGTC_2813|Compound:7971799|FD-Score:3.52|P-value:9.28E-4

Top fitness defect scores for YLR014C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2192 5915882 9.58 198.5 µM 7.64E-18 RNA processing & uracil transport 3 4.68E-22
2 SGTC_1745 st081495 8.46 74.0 µM 2.79E-14 RNA processing & uracil transport 10 1.40E-17
3 SGTC_1348 1493-0373 6.67 93.8 µM 1.59E-9 2 1.30E-11
4 SGTC_1364 1653-0028 6.35 37.6 µM 8.41E-9 3 1.05E-10
5 SGTC_312 4092-0653 6.23 6.8 µM 1.55E-8 ergosterol depletion effects on membrane 37 2.26E-10
6 SGTC_2416 tofa 5.63 880.0 nM 2.93E-7 sphingolipid biosynthesis & PDR1 23 9.01E-9
7 SGTC_138 0850-0259 5.52 40.9 µM 4.80E-7 cell wall 125 1.67E-8
8 SGTC_2313 7726885 5.36 200.0 µM 9.92E-7 RPP1 & pyrimidine depletion 11 4.15E-8
9 SGTC_682 0kpi-0099 5.23 17.0 µM 1.78E-6 32 8.63E-8
10 SGTC_340 k038-0059 5.13 454.0 µM 2.66E-6 ubiquinone biosynthesis & proteasome 28 1.43E-7
11 SGTC_2165 5727197 5.04 32.1 µM 3.93E-6 17 2.33E-7
12 SGTC_321 4534-3890 4.97 65.3 µM 5.33E-6 RPP1 & pyrimidine depletion 1 3.41E-7
13 SGTC_320 4092-0846 4.95 5.3 µM 5.73E-6 ergosterol depletion effects on membrane 24 3.74E-7
14 SGTC_330 1488-0319 4.92 80.1 µM 6.52E-6 RPP1 & pyrimidine depletion 4 4.39E-7
15 SGTC_207 4185-0010 4.76 4.5 µM 1.22E-5 endomembrane recycling 60 9.63E-7
16 SGTC_336 4111-0029 4.60 111.0 µM 2.30E-5 5 2.13E-6
17 SGTC_333 3486-0131 4.53 40.1 µM 3.02E-5 amide catabolism 7 2.99E-6
18 SGTC_2248 tofa 4.28 1.1 µM 7.48E-5 sphingolipid biosynthesis & PDR1 34 9.29E-6
19 SGTC_64 000l-0018 4.00 272.1 µM 2.03E-4 ubiquinone biosynthesis & proteasome 147 3.23E-5
20 SGTC_926 2268-1401 3.94 59.4 µM 2.44E-4 TSC3-RPN4 29 4.07E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.281 4.50E-62 YLR420W URA4 hom Dihydroorotase, catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
0.188 4.93E-28 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.166 3.22E-22 YKR028W SAP190 hom Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
0.160 1.40E-20 YDR290W_d YDR290W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
0.142 1.21E-16 YGR037C ACB1 hom Acyl-CoA-binding protein, transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
0.140 4.17E-16 YNL091W NST1 hom Protein of unknown function, mediates sensitivity to salt stress; interacts physically with the splicing factor Msl1p and also displays genetic interaction with MSL1
0.138 1.19E-15 YLR015W BRE2 hom Subunit of COMPASS (Set1C) complex, which methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
0.137 1.35E-15 YIL104C SHQ1 het Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
0.136 2.24E-15 YAL038W CDC19 het Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
0.136 2.90E-15 YNR012W URK1 hom Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
0.134 6.95E-15 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.131 2.40E-14 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.130 5.06E-14 YER180C-A SLO1 hom Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
0.128 1.09E-13 YDR228C PCF11 het mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping
0.126 2.28E-13 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins