YLR021W / IRC25

Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR021W / IRC25

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:6.37|P-value:4.09E-10 ID:SGTC_64|Compound:000l-0018|FD-Score:14.90|P-value:4.47E-47 ID:SGTC_67|Compound:0335-0847|FD-Score:4.57|P-value:5.28E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:3.89|P-value:8.84E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.44|P-value:4.53E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.38|P-value:5.52E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.22|P-value:2.32E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:4.26|P-value:1.98E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:7.35|P-value:6.61E-13 ID:SGTC_168|Compound:3970-0862|FD-Score:3.32|P-value:6.87E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:7.93|P-value:9.88E-15 ID:SGTC_207|Compound:4185-0010|FD-Score:3.75|P-value:1.47E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.20|P-value:9.91E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.89|P-value:8.87E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.15|P-value:3.13E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.44|P-value:4.56E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.36|P-value:1.18E-7 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.55|P-value:3.17E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-4.09|P-value:4.10E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:-3.34|P-value:6.41E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.20|P-value:2.57E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:3.39|P-value:5.35E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.49|P-value:3.76E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:-3.35|P-value:6.24E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.22|P-value:9.43E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.51|P-value:3.56E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:5.44|P-value:7.77E-8 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.75|P-value:1.51E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.75|P-value:1.51E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.69|P-value:1.87E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:3.58|P-value:2.80E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.83|P-value:1.57E-6 ID:SGTC_1735|Compound:st034310|FD-Score:4.69|P-value:3.05E-6 ID:SGTC_1941|Compound:st076221|FD-Score:-4.89|P-value:1.21E-6 ID:SGTC_2118|Compound:5526350|FD-Score:3.30|P-value:7.30E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-3.38|P-value:5.67E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.51|P-value:6.78E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:10.30|P-value:1.63E-23 ID:SGTC_2348|Compound:9022543|FD-Score:5.51|P-value:5.29E-8 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.97|P-value:6.46E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.46|P-value:4.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.07|P-value:4.46E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.43|P-value:4.74E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-4.21|P-value:2.49E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.85|P-value:1.03E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.74|P-value:4.03E-11 ID:SGTC_2858|Compound:9025781|FD-Score:-3.31|P-value:7.11E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.43|P-value:4.60E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.40|P-value:5.13E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.37|P-value:5.69E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.61|P-value:2.51E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-3.36|P-value:6.10E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:6.37|P-value:4.09E-10 ID:SGTC_64|Compound:000l-0018|FD-Score:14.90|P-value:4.47E-47 ID:SGTC_67|Compound:0335-0847|FD-Score:4.57|P-value:5.28E-6 ID:SGTC_86|Compound:0417-1630|FD-Score:3.89|P-value:8.84E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:3.44|P-value:4.53E-4 ID:SGTC_142|Compound:0335-0849|FD-Score:3.38|P-value:5.52E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.22|P-value:2.32E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:4.26|P-value:1.98E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:7.35|P-value:6.61E-13 ID:SGTC_168|Compound:3970-0862|FD-Score:3.32|P-value:6.87E-4 ID:SGTC_201|Compound:4262-0159|FD-Score:7.93|P-value:9.88E-15 ID:SGTC_207|Compound:4185-0010|FD-Score:3.75|P-value:1.47E-4 ID:SGTC_216|Compound:k038-0035|FD-Score:3.20|P-value:9.91E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.89|P-value:8.87E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.15|P-value:3.13E-5 ID:SGTC_312|Compound:4092-0653|FD-Score:3.44|P-value:4.56E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-5.36|P-value:1.18E-7 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.55|P-value:3.17E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-4.09|P-value:4.10E-5 ID:SGTC_572|Compound:0960-0087|FD-Score:-3.34|P-value:6.41E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:4.20|P-value:2.57E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:3.39|P-value:5.35E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:3.49|P-value:3.76E-4 ID:SGTC_1294|Compound:1000-1494|FD-Score:-3.35|P-value:6.24E-4 ID:SGTC_1325|Compound:1431-0015|FD-Score:-3.22|P-value:9.43E-4 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.51|P-value:3.56E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:5.44|P-value:7.77E-8 ID:SGTC_1340|Compound:1486-1359|FD-Score:-3.75|P-value:1.51E-4 ID:SGTC_1553|Compound:gossypol|FD-Score:3.75|P-value:1.51E-4 ID:SGTC_1554|Compound:st069299|FD-Score:3.69|P-value:1.87E-4 ID:SGTC_1555|Compound:citronellol|FD-Score:3.58|P-value:2.80E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:4.83|P-value:1.57E-6 ID:SGTC_1735|Compound:st034310|FD-Score:4.69|P-value:3.05E-6 ID:SGTC_1941|Compound:st076221|FD-Score:-4.89|P-value:1.21E-6 ID:SGTC_2118|Compound:5526350|FD-Score:3.30|P-value:7.30E-4 ID:SGTC_2121|Compound:5257820|FD-Score:-3.38|P-value:5.67E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.51|P-value:6.78E-6 ID:SGTC_2250|Compound:alverine citrate|FD-Score:10.30|P-value:1.63E-23 ID:SGTC_2348|Compound:9022543|FD-Score:5.51|P-value:5.29E-8 ID:SGTC_2392|Compound:N-(3-chlorophenyl)-6-oxo-1H-pyridine-3-carboxamide|FD-Score:3.66|P-value:2.08E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:3.97|P-value:6.46E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.46|P-value:4.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-4.07|P-value:4.46E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:-3.43|P-value:4.74E-4 ID:SGTC_2622|Compound:2-methoxyxanthone|FD-Score:-4.21|P-value:2.49E-5 ID:SGTC_2624|Compound:nerol|FD-Score:-3.85|P-value:1.03E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.74|P-value:4.03E-11 ID:SGTC_2858|Compound:9025781|FD-Score:-3.31|P-value:7.11E-4 ID:SGTC_3067|Compound:9117431|FD-Score:3.43|P-value:4.60E-4 ID:SGTC_3082|Compound:9117008|FD-Score:3.40|P-value:5.13E-4 ID:SGTC_3102|Compound:9121669|FD-Score:3.37|P-value:5.69E-4 ID:SGTC_3147|Compound:9097027|FD-Score:3.61|P-value:2.51E-4 ID:SGTC_3155|Compound:9099265|FD-Score:-3.36|P-value:6.10E-4

Top fitness defect scores for YLR021W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_64 000l-0018 14.90 272.1 µM 4.47E-47 ubiquinone biosynthesis & proteasome 20 1.89E-50
2 SGTC_2250 alverine citrate 10.30 130.0 µM 1.63E-23 8 3.96E-25
3 SGTC_201 4262-0159 7.93 12.8 µM 9.88E-15 ergosterol depletion effects on membrane 10 1.07E-15
4 SGTC_165 k038-0023 7.35 64.4 µM 6.61E-13 ubiquinone biosynthesis & proteasome 12 9.78E-14
5 SGTC_2688 kanamycin b 6.74 96.7 µM 4.03E-11 calcium & mitochondrial duress 118 8.05E-12
6 SGTC_36 0335-0881 6.37 14.2 µM 4.09E-10 ubiquinone biosynthesis & proteasome 56 9.70E-11
7 SGTC_2348 9022543 5.51 33.4 µM 5.29E-8 mitochondrial processes 9 1.79E-8
8 SGTC_1338 1486-1223 5.44 76.0 µM 7.77E-8 2 2.70E-8
9 SGTC_1597 7-benzyloxygramine 4.83 47.5 µM 1.57E-6 calcium & mitochondrial duress 73 6.76E-7
10 SGTC_1735 st034310 4.69 56.8 µM 3.05E-6 Golgi 13 1.38E-6
11 SGTC_67 0335-0847 4.57 266.0 µM 5.28E-6 ubiquinone biosynthesis & proteasome 93 2.49E-6
12 SGTC_2249 haloperidol 4.51 116.4 µM 6.78E-6 ergosterol depletion effects on membrane 80 3.25E-6
13 SGTC_160 3453-0671 4.26 289.7 µM 1.98E-5 30 1.02E-5
14 SGTC_158 k038-0087 4.22 26.4 µM 2.32E-5 ubiquinone biosynthesis & proteasome 125 1.21E-5
15 SGTC_731 3966-0296 4.20 387.0 µM 2.57E-5 12 1.35E-5
16 SGTC_2409 alverine citrate 3.97 64.0 µM 6.46E-5 49 3.63E-5
17 SGTC_86 0417-1630 3.89 471.9 µM 8.84E-5 ubiquinone biosynthesis & proteasome 157 5.09E-5
18 SGTC_207 4185-0010 3.75 4.5 µM 1.47E-4 endomembrane recycling 96 8.75E-5
19 SGTC_1553 gossypol 3.75 38.6 µM 1.51E-4 3 9.01E-5
20 SGTC_1554 st069299 3.69 38.7 µM 1.87E-4 31 1.13E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.394 1.32E-124 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.379 1.02E-114 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.370 4.89E-109 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.359 1.76E-102 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.358 6.18E-102 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.355 2.82E-100 YPL144W POC4 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.351 1.19E-97 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
0.341 6.70E-92 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.340 1.03E-91 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.336 2.34E-89 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.335 1.79E-88 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.325 1.83E-83 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.322 1.96E-81 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.321 7.30E-81 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.314 9.39E-78 YNL111C CYB5 hom Cytochrome b5, involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation