YLR028C / ADE16

Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR028C / ADE16

Click on Significant Values for Screen Details ID:SGTC_158|Compound:k038-0087|FD-Score:3.68|P-value:4.90E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:4.53|P-value:8.31E-7 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.85|P-value:1.18E-7 ID:SGTC_493|Compound:zm226600|FD-Score:-3.65|P-value:4.85E-5 ID:SGTC_647|Compound:0140-0236|FD-Score:2.99|P-value:7.81E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:3.86|P-value:2.24E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:-3.28|P-value:2.26E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.44|P-value:1.36E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:-2.91|P-value:9.19E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:-4.50|P-value:7.94E-7 ID:SGTC_877|Compound:0771-0151|FD-Score:-2.90|P-value:9.55E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.07|P-value:5.20E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.01|P-value:7.46E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.31|P-value:2.35E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.36|P-value:1.68E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.47|P-value:9.18E-7 ID:SGTC_1465|Compound:k213-0056|FD-Score:-4.63|P-value:3.88E-7 ID:SGTC_1628|Compound:st004252|FD-Score:3.31|P-value:2.36E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.71|P-value:4.44E-5 ID:SGTC_1738|Compound:st035555|FD-Score:-3.22|P-value:2.93E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.56|P-value:8.49E-5 ID:SGTC_1916|Compound:st067606|FD-Score:-4.01|P-value:9.35E-6 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.94|P-value:7.03E-8 ID:SGTC_2041|Compound:5107442|FD-Score:-3.15|P-value:3.85E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.17|P-value:3.56E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.84|P-value:2.03E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.07|P-value:5.82E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-4.05|P-value:7.71E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.51|P-value:8.74E-5 ID:SGTC_2769|Compound:toremifene|FD-Score:5.58|P-value:1.71E-9 ID:SGTC_2781|Compound:5567276|FD-Score:3.73|P-value:4.06E-5 ID:SGTC_2930|Compound:9013230|FD-Score:4.05|P-value:9.27E-6 ID:SGTC_2932|Compound:9016711|FD-Score:-4.46|P-value:1.00E-6 ID:SGTC_3134|Compound:9127454|FD-Score:2.93|P-value:9.97E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.16|P-value:5.36E-6 ID:SGTC_3311|Compound:9131146|FD-Score:3.06|P-value:6.12E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:3.68|P-value:4.90E-5 ID:SGTC_165|Compound:k038-0023|FD-Score:4.53|P-value:8.31E-7 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.85|P-value:1.18E-7 ID:SGTC_493|Compound:zm226600|FD-Score:-3.65|P-value:4.85E-5 ID:SGTC_647|Compound:0140-0236|FD-Score:2.99|P-value:7.81E-4 ID:SGTC_650|Compound:1328-0195|FD-Score:3.86|P-value:2.24E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:-3.28|P-value:2.26E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:3.44|P-value:1.36E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:-2.91|P-value:9.19E-4 ID:SGTC_748|Compound:0136-0228|FD-Score:-4.50|P-value:7.94E-7 ID:SGTC_877|Compound:0771-0151|FD-Score:-2.90|P-value:9.55E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:-3.07|P-value:5.20E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.01|P-value:7.46E-4 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.31|P-value:2.35E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.36|P-value:1.68E-6 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.47|P-value:9.18E-7 ID:SGTC_1465|Compound:k213-0056|FD-Score:-4.63|P-value:3.88E-7 ID:SGTC_1628|Compound:st004252|FD-Score:3.31|P-value:2.36E-4 ID:SGTC_1728|Compound:st035614|FD-Score:3.71|P-value:4.44E-5 ID:SGTC_1738|Compound:st035555|FD-Score:-3.22|P-value:2.93E-4 ID:SGTC_1783|Compound:st039459|FD-Score:3.56|P-value:8.49E-5 ID:SGTC_1916|Compound:st067606|FD-Score:-4.01|P-value:9.35E-6 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.94|P-value:7.03E-8 ID:SGTC_2041|Compound:5107442|FD-Score:-3.15|P-value:3.85E-4 ID:SGTC_2167|Compound:5876490|FD-Score:-3.17|P-value:3.56E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.84|P-value:2.03E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.07|P-value:5.82E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-4.05|P-value:7.71E-6 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:-3.51|P-value:8.74E-5 ID:SGTC_2769|Compound:toremifene|FD-Score:5.58|P-value:1.71E-9 ID:SGTC_2781|Compound:5567276|FD-Score:3.73|P-value:4.06E-5 ID:SGTC_2930|Compound:9013230|FD-Score:4.05|P-value:9.27E-6 ID:SGTC_2932|Compound:9016711|FD-Score:-4.46|P-value:1.00E-6 ID:SGTC_3134|Compound:9127454|FD-Score:2.93|P-value:9.97E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.16|P-value:5.36E-6 ID:SGTC_3311|Compound:9131146|FD-Score:3.06|P-value:6.12E-4

Top fitness defect scores for YLR028C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2769 toremifene 5.58 3.7 µM 1.71E-9 5 1.21E-8
2 SGTC_165 k038-0023 4.53 64.4 µM 8.31E-7 ubiquinone biosynthesis & proteasome 58 3.00E-6
3 SGTC_3198 9112019 4.16 49.5 µM 5.36E-6 mitochondrial processes 12 1.59E-5
4 SGTC_2930 9013230 4.05 14.9 µM 9.27E-6 heme biosynthesis & mitochondrial translocase 34 2.59E-5
5 SGTC_650 1328-0195 3.86 38.0 µM 2.24E-5 21 5.70E-5
6 SGTC_2781 5567276 3.73 71.4 µM 4.06E-5 56 9.72E-5
7 SGTC_1728 st035614 3.71 33.8 µM 4.44E-5 64 1.05E-4
8 SGTC_158 k038-0087 3.68 26.4 µM 4.90E-5 ubiquinone biosynthesis & proteasome 160 1.15E-4
9 SGTC_1783 st039459 3.56 87.5 µM 8.49E-5 Golgi 33 1.88E-4
10 SGTC_725 1497-0963 3.44 19.0 µM 1.36E-4 copper-dependent oxidative stress 33 2.86E-4
11 SGTC_1077 5-fluorocytosine 3.31 377.0 nM 2.35E-4 exosome 37 4.69E-4
12 SGTC_1628 st004252 3.31 77.7 µM 2.36E-4 45 4.70E-4
13 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 3.07 13.2 µM 5.82E-4 65 0.00106
14 SGTC_3311 9131146 3.06 2.9 µM 6.12E-4 cell wall 170 0.00111
15 SGTC_1067 clotrimazole 3.01 1.4 µM 7.46E-4 azole & statin 85 0.00132
16 SGTC_647 0140-0236 2.99 81.0 µM 7.81E-4 42 0.00138
17 SGTC_3134 9127454 2.93 49.5 µM 9.97E-4 43 0.00171
18 SGTC_449 xestospongin c 2.88 11.2 µM 0.00116 NEO1-PIK1 136 0.00196
19 SGTC_1214 0358-0031 2.81 63.7 µM 0.00151 75 0.00249
20 SGTC_3140 9094243 2.78 49.5 µM 0.00168 94 0.00273

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 1.56E-31 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.193 1.71E-29 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.156 1.19E-19 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.155 1.41E-19 YOR023C AHC1 hom Subunit of the Ada histone acetyltransferase complex, required for structural integrity of the complex
0.140 4.18E-16 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.139 7.72E-16 YLR316C TAD3 het Subunit of tRNA-specific adenosine-34 deaminase, forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs
0.138 9.32E-16 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.137 1.60E-15 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.133 1.17E-14 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.130 4.53E-14 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.128 9.68E-14 YLR385C SWC7 hom Protein of unknown function, component of the Swr1p complex that incorporates Htz1p into chromatin
0.125 3.10E-13 YLR353W BUD8 hom Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole
0.124 4.80E-13 YJR127C RSF2 hom Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.118 5.76E-12 YCL035C GRX1 hom Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.116 1.38E-11 YBR003W COQ1 hom Hexaprenyl pyrophosphate synthetase, catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis