YLR032W / RAD5

DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YLR032W / RAD5

Click on Significant Values for Screen Details ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.37|P-value:4.56E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:4.39|P-value:1.04E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.37|P-value:5.44E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:10.10|P-value:5.35E-23 ID:SGTC_171|Compound:k064-0041|FD-Score:5.09|P-value:3.84E-7 ID:SGTC_223|Compound:busulfan|FD-Score:4.09|P-value:3.68E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.33|P-value:1.35E-5 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.54|P-value:5.35E-6 ID:SGTC_248|Compound:streptozotocin|FD-Score:8.63|P-value:2.30E-17 ID:SGTC_251|Compound:melphalan|FD-Score:7.58|P-value:8.79E-14 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.00|P-value:1.97E-42 ID:SGTC_313|Compound:3474-0040|FD-Score:-5.59|P-value:2.10E-8 ID:SGTC_318|Compound:1488-0629|FD-Score:3.82|P-value:1.09E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.86|P-value:7.45E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:-4.90|P-value:7.61E-7 ID:SGTC_419|Compound:nsc-638432|FD-Score:-4.36|P-value:9.20E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:8.17|P-value:9.78E-16 ID:SGTC_550|Compound:4123-0385|FD-Score:7.97|P-value:4.49E-15 ID:SGTC_574|Compound:1315-0397|FD-Score:3.50|P-value:3.49E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.24|P-value:8.60E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.31|P-value:6.89E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.52|P-value:3.28E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.35|P-value:1.20E-5 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.06|P-value:4.11E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:7.17|P-value:1.58E-12 ID:SGTC_958|Compound:1222-0007|FD-Score:6.17|P-value:9.91E-10 ID:SGTC_974|Compound:1326-0144|FD-Score:5.27|P-value:1.55E-7 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.69|P-value:2.68E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.56|P-value:2.86E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.27|P-value:7.87E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:6.13|P-value:1.28E-9 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.86|P-value:9.04E-5 ID:SGTC_1425|Compound:4048-4433|FD-Score:5.62|P-value:2.41E-8 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.53|P-value:5.65E-6 ID:SGTC_1741|Compound:st038134|FD-Score:-3.30|P-value:5.97E-4 ID:SGTC_1761|Compound:st044296|FD-Score:3.20|P-value:9.73E-4 ID:SGTC_1840|Compound:st055363|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:7.90|P-value:7.56E-15 ID:SGTC_1921|Compound:5740136|FD-Score:4.74|P-value:2.16E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_1936|Compound:st074407|FD-Score:14.20|P-value:1.52E-43 ID:SGTC_1945|Compound:st075991|FD-Score:3.89|P-value:8.15E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.26|P-value:8.01E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.91|P-value:7.54E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.40|P-value:4.94E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.97|P-value:5.92E-5 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:6.42|P-value:2.15E-10 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-5.49|P-value:3.70E-8 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.22|P-value:2.10E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.40|P-value:5.03E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.73|P-value:1.52E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:7.02|P-value:4.52E-12 ID:SGTC_2817|Compound:7995342|FD-Score:4.15|P-value:2.83E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.69|P-value:1.78E-4 ID:SGTC_2849|Compound:9019321|FD-Score:8.36|P-value:2.14E-16 ID:SGTC_2922|Compound:7999257|FD-Score:4.82|P-value:1.44E-6 ID:SGTC_2929|Compound:9013348|FD-Score:3.91|P-value:7.54E-5 ID:SGTC_2936|Compound:9038235|FD-Score:5.85|P-value:6.48E-9 ID:SGTC_3016|Compound:9080866|FD-Score:4.56|P-value:4.75E-6 ID:SGTC_3131|Compound:9127911|FD-Score:3.23|P-value:8.95E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.19|P-value:8.48E-4 ID:SGTC_3138|Compound:9097216|FD-Score:4.84|P-value:1.30E-6 ID:SGTC_3139|Compound:9095020|FD-Score:-3.29|P-value:6.05E-4 ID:SGTC_3170|Compound:9103054|FD-Score:4.42|P-value:9.23E-6 ID:SGTC_3171|Compound:9103192|FD-Score:3.37|P-value:5.51E-4 ID:SGTC_3172|Compound:9105863|FD-Score:3.61|P-value:2.33E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-9.25|P-value:7.25E-20 ID:SGTC_3254|Compound:9136651|FD-Score:4.87|P-value:1.15E-6 ID:SGTC_28|Compound:pentachloropyridine N-oxide|FD-Score:-3.37|P-value:4.56E-4 ID:SGTC_133|Compound:0724-0033|FD-Score:4.39|P-value:1.04E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.37|P-value:5.44E-4 ID:SGTC_170|Compound:4197-0004|FD-Score:10.10|P-value:5.35E-23 ID:SGTC_171|Compound:k064-0041|FD-Score:5.09|P-value:3.84E-7 ID:SGTC_223|Compound:busulfan|FD-Score:4.09|P-value:3.68E-5 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.33|P-value:1.35E-5 ID:SGTC_247|Compound:mechlorethamine|FD-Score:4.54|P-value:5.35E-6 ID:SGTC_248|Compound:streptozotocin|FD-Score:8.63|P-value:2.30E-17 ID:SGTC_251|Compound:melphalan|FD-Score:7.58|P-value:8.79E-14 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:14.00|P-value:1.97E-42 ID:SGTC_313|Compound:3474-0040|FD-Score:-5.59|P-value:2.10E-8 ID:SGTC_318|Compound:1488-0629|FD-Score:3.82|P-value:1.09E-4 ID:SGTC_364|Compound:0083-0097|FD-Score:-3.86|P-value:7.45E-5 ID:SGTC_370|Compound:0180-0345|FD-Score:-4.90|P-value:7.61E-7 ID:SGTC_419|Compound:nsc-638432|FD-Score:-4.36|P-value:9.20E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:8.17|P-value:9.78E-16 ID:SGTC_550|Compound:4123-0385|FD-Score:7.97|P-value:4.49E-15 ID:SGTC_574|Compound:1315-0397|FD-Score:3.50|P-value:3.49E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.24|P-value:8.60E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:3.31|P-value:6.89E-4 ID:SGTC_794|Compound:k038-0031|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.52|P-value:3.28E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:4.35|P-value:1.20E-5 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:4.06|P-value:4.11E-5 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:7.17|P-value:1.58E-12 ID:SGTC_958|Compound:1222-0007|FD-Score:6.17|P-value:9.91E-10 ID:SGTC_974|Compound:1326-0144|FD-Score:5.27|P-value:1.55E-7 ID:SGTC_1031|Compound:k035-0009|FD-Score:4.69|P-value:2.68E-6 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.56|P-value:2.86E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.27|P-value:7.87E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:6.13|P-value:1.28E-9 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.86|P-value:9.04E-5 ID:SGTC_1425|Compound:4048-4433|FD-Score:5.62|P-value:2.41E-8 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.53|P-value:5.65E-6 ID:SGTC_1741|Compound:st038134|FD-Score:-3.30|P-value:5.97E-4 ID:SGTC_1761|Compound:st044296|FD-Score:3.20|P-value:9.73E-4 ID:SGTC_1840|Compound:st055363|FD-Score:3.55|P-value:2.88E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:7.90|P-value:7.56E-15 ID:SGTC_1921|Compound:5740136|FD-Score:4.74|P-value:2.16E-6 ID:SGTC_1928|Compound:4'-methoxychalcone|FD-Score:3.54|P-value:2.99E-4 ID:SGTC_1936|Compound:st074407|FD-Score:14.20|P-value:1.52E-43 ID:SGTC_1945|Compound:st075991|FD-Score:3.89|P-value:8.15E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.26|P-value:8.01E-4 ID:SGTC_2080|Compound:5212524|FD-Score:-3.60|P-value:2.05E-4 ID:SGTC_2121|Compound:5257820|FD-Score:3.91|P-value:7.54E-5 ID:SGTC_2318|Compound:6029776|FD-Score:3.40|P-value:4.94E-4 ID:SGTC_2326|Compound:6164455|FD-Score:3.97|P-value:5.92E-5 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:6.42|P-value:2.15E-10 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-5.49|P-value:3.70E-8 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.22|P-value:2.10E-5 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.40|P-value:5.03E-4 ID:SGTC_2611|Compound:pyromycin|FD-Score:3.73|P-value:1.52E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:7.02|P-value:4.52E-12 ID:SGTC_2817|Compound:7995342|FD-Score:4.15|P-value:2.83E-5 ID:SGTC_2835|Compound:9006588|FD-Score:3.69|P-value:1.78E-4 ID:SGTC_2849|Compound:9019321|FD-Score:8.36|P-value:2.14E-16 ID:SGTC_2922|Compound:7999257|FD-Score:4.82|P-value:1.44E-6 ID:SGTC_2929|Compound:9013348|FD-Score:3.91|P-value:7.54E-5 ID:SGTC_2936|Compound:9038235|FD-Score:5.85|P-value:6.48E-9 ID:SGTC_3016|Compound:9080866|FD-Score:4.56|P-value:4.75E-6 ID:SGTC_3131|Compound:9127911|FD-Score:3.23|P-value:8.95E-4 ID:SGTC_3136|Compound:9095672|FD-Score:-3.19|P-value:8.48E-4 ID:SGTC_3138|Compound:9097216|FD-Score:4.84|P-value:1.30E-6 ID:SGTC_3139|Compound:9095020|FD-Score:-3.29|P-value:6.05E-4 ID:SGTC_3170|Compound:9103054|FD-Score:4.42|P-value:9.23E-6 ID:SGTC_3171|Compound:9103192|FD-Score:3.37|P-value:5.51E-4 ID:SGTC_3172|Compound:9105863|FD-Score:3.61|P-value:2.33E-4 ID:SGTC_3201|Compound:9113999|FD-Score:3.58|P-value:2.61E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-9.25|P-value:7.25E-20 ID:SGTC_3254|Compound:9136651|FD-Score:4.87|P-value:1.15E-6

Top fitness defect scores for YLR032W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1936 st074407 14.20 74.3 µM 1.52E-43 DNA damage response 1 8.56E-46
2 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 14.00 44.4 nM 1.97E-42 DNA damage response 10 1.27E-44
3 SGTC_170 4197-0004 10.10 226.0 µM 5.35E-23 1 3.56E-24
4 SGTC_248 streptozotocin 8.63 5.2 mM 2.30E-17 DNA damage response 4 3.04E-18
5 SGTC_2849 9019321 8.36 13.0 µM 2.14E-16 5 3.17E-17
6 SGTC_429 1112-0049 8.17 25.5 µM 9.78E-16 DNA damage response 5 1.57E-16
7 SGTC_550 4123-0385 7.97 25.7 µM 4.49E-15 TSC3-RPN4 4 7.83E-16
8 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 7.90 62.6 µM 7.56E-15 TSC3-RPN4 4 1.35E-15
9 SGTC_251 melphalan 7.58 456.5 µM 8.79E-14 DNA damage response 3 1.79E-14
10 SGTC_915 methyl methanesulfonate (MMS) 7.17 110.0 mM 1.58E-12 DNA damage response 5 3.74E-13
11 SGTC_2677 mitomycin C 7.02 100.0 µM 4.52E-12 DNA damage response 2 1.14E-12
12 SGTC_2535 4',4'-dimethoxydalbergione 6.42 51.2 µM 2.15E-10 DNA damage response 4 6.63E-11
13 SGTC_958 1222-0007 6.17 33.0 µM 9.91E-10 7 3.32E-10
14 SGTC_1298 1079-1278 6.13 263.0 µM 1.28E-9 DNA damage response 1 4.33E-10
15 SGTC_2936 9038235 5.85 15.6 µM 6.48E-9 4 2.40E-9
16 SGTC_1425 4048-4433 5.62 7.3 µM 2.41E-8 copper-dependent oxidative stress 12 9.58E-9
17 SGTC_974 1326-0144 5.27 138.0 µM 1.55E-7 ERG2 17 6.79E-8
18 SGTC_171 k064-0041 5.09 9.7 µM 3.84E-7 heme requiring 13 1.77E-7
19 SGTC_3254 9136651 4.87 49.5 µM 1.15E-6 DNA damage response 12 5.60E-7
20 SGTC_3138 9097216 4.84 49.5 µM 1.30E-6 7 6.40E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.410 2.25E-136 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.399 2.28E-128 YDR092W UBC13 hom Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
0.384 1.95E-118 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.295 2.57E-68 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.273 2.88E-58 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.272 4.50E-58 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.263 2.53E-54 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.238 2.26E-44 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.237 3.75E-44 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.234 6.25E-43 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.233 2.28E-42 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.228 7.62E-41 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.203 1.08E-32 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.186 2.08E-27 YLR376C PSY3 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
0.169 5.95E-23 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p